CN106399053B - A kind of PCR reaction unit and its application method - Google Patents
A kind of PCR reaction unit and its application method Download PDFInfo
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- CN106399053B CN106399053B CN201611024477.9A CN201611024477A CN106399053B CN 106399053 B CN106399053 B CN 106399053B CN 201611024477 A CN201611024477 A CN 201611024477A CN 106399053 B CN106399053 B CN 106399053B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/042—Caps; Plugs
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/046—Function or devices integrated in the closure
- B01L2300/048—Function or devices integrated in the closure enabling gas exchange, e.g. vents
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- Devices And Processes Conducted In The Presence Of Fluids And Solid Particles (AREA)
Abstract
The present invention provides a kind of PCR reaction unit, the PCR reaction unit realizes PCR reaction unit and extraneous connection by the way that first through hole and the second through-hole are arranged on semiclosed channel, reaction system can be entered in PCR reaction tube by through-hole, another through-hole that reaction system does not flow through realizes inside and outside air pressure balance of the PCR reaction unit under semi-closed state, realizes PCR reaction unit and the connection of front reaction device and the conveying of reaction system.The present invention also provides the application methods of PCR reaction unit, tubular body enters the disengaging of pressing seal head realization tubular body behind semiclosed channel, to realize closing and opening of the tubular body to through-hole, simplify the closed process of PCR reaction unit, being enclosed under craft and mechanical system for PCR reaction unit can be realized easily.PCR reaction unit structure provided by the invention is simple, easily prepared, can effectively realize the semiclosed of PCR reaction unit, and operating method is easy, is easy to carry out.
Description
Technical field
The present invention relates to PCR reaction unit technical field more particularly to a kind of PCR reaction unit and its application methods.
Background technique
PCR (Polymerase Chain Reaction, polymerase chain reaction) is that a kind of amplify in vitro expands
The technology of specific DNA (deoxyribonucleic acid, DNA) segment.PCR is generally required in DNA amplification
By three high temperature solution chain reaction, annealing reaction and extension steps, the initial DNA molecular quantity after above three step
It doubles, is recycled at this time for one；DNA molecular after multiplication then becomes the template of next circulation, so circulation multiplication
Go down, after 30-40 circulation, DNA molecular number will be amplified to nearly 109 times of initial value.It is directed to analysis and inspection as a result,
DNA molecular as analyte can be carried out the extensive amplification of specificity by the purpose tested, PCR, and therefore, round pcr can
For the early diagnosis of infectious disease, genetic disease and tumour etc., meanwhile, it is obtained in antenatal exaination and forensic identification increasingly wider
General application.
The detection process of the molecular detecting method of based on PCR technology is substantially are as follows: sample process, nucleic acid extraction and PCR are anti-
Answer liquid mixing and etc. rear formation PCR reaction system, PCR reaction system is transferred in dedicated PCR reaction unit, PCR is anti-
Pcr amplification reaction and signal detection etc. are carried out after answering device to close.In above-mentioned detection process, PCR reaction system forming process
Mixing, the transfer of middle solution, the processes such as pcr amplification reaction and signal detection are generally all in EP (Epoxy epoxide, asphalt mixtures modified by epoxy resin
Rouge) it carries out in pipe, since PCR reaction system is in open state before being transferred to PCR reaction unit, this reacts PCR
Device is easy by extraneous pollution, and then is possible to pollution PCR reaction system.Simultaneously as the pipe lid structure of EP pipe makes
The lid of EP pipe, which closes, to be operated using manual mode, this is not only time-consuming and laborious, and be not met by modernization, quickly
Testing requirements.
Currently, the automation process for lid of uncapping/close in PCR detection process is mainly reacted using a kind of cap-rotating type PCR
Pipe, cap-rotating type PCR reaction tube are typically used on large-scale work station.When using cap-rotating type PCR reaction tube, by utilizing step
Lid manipulator crawl lid is grabbed into motor driven and screws lid after lid is navigated to PCR reaction tube position directly above；It uncaps
When the step of it is opposite.Crawl due to grabbing lid manipulator needs very accurate positioning and control system intervention, thus PCR reacts
High-accuracy, high quality equipment is needed during system preparation and transfer, this cost that PCR is detected improves.EP pipe and
Cap-rotating type PCR reaction tube can be only in completely open or completely enclosed state, thus PCR reaction system system in operation
It needs to complete outputting and inputting for liquid by external medium such as pipettor during standby and transfer.
Summary of the invention
The present invention provides a kind of PCR reaction unit and its application method, to solve existing PCR reaction unit in operating process
It is middle to need to complete the problem of outputting and inputting of liquid by external medium.
The present invention provides a kind of PCR reaction unit, the PCR reaction unit include the internal PCR reaction tube being connected and
First through hole and the second through-hole is respectively set on opposite two sidewalls in semiclosed channel, the semiclosed channel；The PCR reaction
Device further includes piston, and the piston includes tubular body and seal head, the inner cavity phase of the tubular body and the semiclosed channel
Matching, the end face of the seal head are inclined-plane；The length of the tubular body is greater than the first through hole and the semiclosed channel
The distance between opening, the length of the tubular body be greater than between second through-hole and the semiclosed access portal away from
From.
Preferably, the opposite first through hole in position and institute is respectively set on the opposite two sidewalls in the semiclosed channel
State the second through-hole.
Preferably, the inner cavity in the semiclosed channel is in the axial direction from the semiclosed access portal to the PCR
Reaction tube direction is gradually reduced.
Preferably, the inner cavity in the semiclosed channel is round, oval, diamond shape or rectangular in radial directions.
Preferably, shape is identical in radial directions with the tubular body for the seal head；The radial ruler of the seal head
The very little radial dimension greater than the tubular body.
Preferably, the PCR reaction tube is tubulose or flat reaction tube.
Preferably, the PCR reaction unit further include closing wedge, the closing wedge include be sequentially connected the connector connect,
Pressing piece and extrusion, the semiclosed channel, which is not provided on the side wall of the first through hole and second through-hole, is arranged institute
State connector；The end face of the extrusion is inclined-plane.
Preferably, the bevel inclination angle of the extrusion is identical as the bevel inclination angle of the seal head.
The present invention provides a kind of application method of PCR reaction unit, and the application method includes:
The tubular body of piston is put into semiclosed channel, and the tubular body does not close first through hole and the second through-hole；
The first through hole or second through-hole are connect by pipeline with the device for being contained with PCR reaction system, with
The PCR reaction system is set to enter PCR reaction tube from the first through hole or second through-hole；
The PCR reaction system enters pressing closing wedge after the PCR reaction tube, so that tubular body closing described the
One through-hole and second through-hole.
The technical solution that the embodiment of the present invention provides can include the following benefits:
The present invention provides a kind of PCR reaction unit, the PCR reaction unit include the internal PCR reaction tube being connected and
First through hole and the second through-hole is respectively set on opposite two sidewalls in semiclosed channel, the semiclosed channel；The PCR reaction
Device further includes piston, and the piston includes tubular body and seal head, the inner cavity phase of the tubular body and the semiclosed channel
Matching, the end face of the seal head are inclined-plane；The length of the tubular body is greater than the first through hole and the semiclosed channel
The distance between opening, the length of the tubular body be greater than between second through-hole and the semiclosed access portal away from
From.PCR reaction unit provided by the invention is led to by the way that first through hole and second is arranged on the opposite two sidewalls in semiclosed channel
Hole can be realized PCR reaction unit and extraneous connection, and PCR reaction system after mixing can pass through first through hole or the
Two through-holes enter in PCR reaction tube, and another through-hole that PCR reaction system does not flow through then realizes PCR reaction unit in semiclosed shape
The inside and outside air pressure balance kept under state, and then realize the connection and PCR reaction system of PCR reaction unit and front reaction device
Conveying.Meanwhile the present invention also provides the application method of PCR reaction unit, the tubular body of piston enters semiclosed channel, passes through
Pressing seal head realizes that the disengaging of tubular body makes to realize closing and opening of the tubular body to first through hole and the second through-hole
The closed process for obtaining PCR reaction unit simplifies, and then being enclosed under manual mode and mechanical system for PCR reaction unit can be light
Easily realize.PCR reaction unit structure provided by the invention is simple, easily prepared, can effectively realize half envelope of PCR reaction unit
It closes, and operating method is easy, is easy to carry out.
It should be understood that above general description and following detailed description be only it is exemplary and explanatory, not
It can the limitation present invention.
Detailed description of the invention
The drawings herein are incorporated into the specification and forms part of this specification, and shows and meets implementation of the invention
Example, and be used to explain the principle of the present invention together with specification.
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, for those of ordinary skill in the art
Speech, without any creative labor, is also possible to obtain other drawings based on these drawings.
Fig. 1 is the schematic perspective view of PCR reaction unit provided in an embodiment of the present invention；
Fig. 2 is the schematic perspective view in semiclosed channel in PCR reaction unit provided in an embodiment of the present invention；
Fig. 3 is the sectional view in the schematic perspective view of PCR reaction unit provided in an embodiment of the present invention at A-A；
Fig. 4 is the schematic perspective view of piston in PCR reaction unit provided in an embodiment of the present invention；
Symbol indicates:
1-PCR reaction tube, the semiclosed channel 2-, 3- first through hole, the second through-hole of 4-, 5- piston, 51- tubular body, 52- are close
End socket, 6- close wedge, 61- connector, 62- pressing piece, 63- extrusion.
Specific embodiment
Example embodiments are described in detail here, and the example is illustrated in the accompanying drawings.Following description is related to
When attached drawing, unless otherwise indicated, the same numbers in different drawings indicate the same or similar elements.Following exemplary embodiment
Described in embodiment do not represent all embodiments consistented with the present invention.On the contrary, they be only with it is such as appended
The example of device and method being described in detail in claims, some aspects of the invention are consistent.
Attached drawing 1 is please referred to, attached drawing 1 shows the schematic perspective view of PCR reaction unit provided in an embodiment of the present invention.
PCR reaction unit provided in an embodiment of the present invention includes PCR reaction tube 1, semiclosed channel 2, piston 5 and closing wedge
6, it is specific to describe are as follows:
PCR reaction tube 1 it is closed at one end, the other end is connected with semiclosed channel 2, is connected with semiclosed channel 2
One end is for receiving PCR reaction system.The inside of PCR reaction tube 1 has certain cavity, and the cavity is for holding PCR reaction
System.The shape of PCR reaction tube 1 can preferably adopt in embodiments of the present invention for tubulose, flat, round, rectangular etc.
With tubulose or flat PCR reaction tube 1, so that the PCR reaction system of PCR reaction tube 1 can be heated in PCR amplification
It is even, to improve the amplification rate of DNA.
Semiclosed channel 2 is the component led in inside, and semiclosed channel 2 enters PCR reaction tube 1 for PCR reaction system and mentions
For channel.The one end in semiclosed channel 2 is connected with PCR reaction tube 1, and the other end opens wide, and the open one end in semiclosed channel 2
For placing movable piston 5.It is respectively set first through hole 3 and the second through-hole 4 on the opposite two sidewalls in semiclosed channel 2, first
Through-hole 3 and the second through-hole 4 can be realized PCR reaction system and enter in PCR reaction tube 1, and then realize PCR reaction unit and front end
The connection of reaction unit and the conveying of PCR reaction system；Meanwhile first through hole 3 and the second through-hole 4 can also realize that PCR reacts
Device inside and extraneous connection, and then realize inside and outside air pressure balance of the PCR reaction unit under semi-closed state.In general, the
The aperture of one through-hole 3 and the second through-hole 4 is set as 1-2mm.
Piston 5 includes 52 two components of tubular body 51 and seal head, wherein tubular body 51 is for closing 3 He of first through hole
Second through-hole 4, and then PCR reaction unit is made to be in closed state；The setting of seal head 52 can be convenient for tubular body 51 to first
The closing of through-hole 3 and the second through-hole 4.To be closed convenient for tubular body 51 to first through hole 3 and the second through-hole 4, tubular body 51
Length be greater than first through hole 3 and semiclosed channel 2 opening the distance between, meanwhile, the length of tubular body 51 is also greater than
The distance between second through-hole 4 and semiclosed channel 2 opening.The outer shape and size of tubular body 51 are required to lead to semiclosed
The inner cavity in road 2 matches, so as to enter PCR reaction tube 1 in the case where tubular body 51 closes first through hole 3 and the second through-hole 4
Interior PCR reaction system will not between tubular body 51 and semiclosed channel 2 gap outflow.For convenient for smoothly by tubular body 51
Be squeezed into semiclosed channel 2, the end face of seal head 52 is set as inclined-plane so that the thrust of Level Promoting piston 5 be converted into it is perpendicular
Histogram to.The tilt angle on 52 inclined-plane of seal head is according to the mobile horizontal distance of piston 5,5 height of piston and the thickness for closing wedge 6
It is calculated, thus, for various sizes of PCR reaction unit, the tilt angle on 52 inclined-plane of seal head is also just different.
Closing wedge 6 includes being sequentially connected the connector 61, pressing piece 62 and extrusion 63 connect, wherein semiclosed channel 2 is not
It is provided with setting connector 61 on the side wall of first through hole 3 and the second through-hole 4, i.e. 2 side of semiclosed channel where connector 61
Wall is adjacent with the side wall where through-hole.The end face of extrusion 63 is inclined-plane, which is in contact with the inclined-plane of piston 5, and two is oblique
The tilt angle in face is identical.Wedge 6 is closed when in use, by pushing pressing piece 62 to make the inclined-plane of extrusion 63 and piston 5
Inclined-plane is in contact, so that piston 5 enters in semiclosed channel 2 under the motive force of closing wedge 6, completes piston 5 and seals to half
The closing of closed walk 2.Since the structure of closing wedge 6 and piston 5 is simple, is easy to close, thus it can be used manual mode real easily
The closing of existing PCR reaction unit, while the closing of PCR reaction unit can also be realized using simple mechanical device.
PCR reaction unit provided in an embodiment of the present invention is logical by the way that first is arranged on the opposite two sidewalls in semiclosed channel
Hole and the second through-hole can be realized PCR reaction unit and extraneous connection, and PCR reaction system after mixing can be by the
One through-hole or the second through-hole enter in PCR reaction tube, and another through-hole that PCR reaction system does not flow through then realizes PCR reaction unit
The inside and outside air pressure balance kept under semi-closed state, and then realize connection and the PCR of PCR reaction unit and front reaction device
The conveying of reaction system.PCR reaction unit structure provided in an embodiment of the present invention is simple, easily prepared, can effectively realize PCR
Reaction unit it is semiclosed, and operating method is easy, is easy to carry out.
Please refer to attached drawing 2, attached drawing 2 shows the vertical of semiclosed channel in PCR reaction unit provided in an embodiment of the present invention
Body structural schematic diagram.
First through hole 3 and second is provided on two sides opposite from attached drawing 2, it can be seen that semiclosed channel 2 to lead to
Hole 4, to realize conveying, the output of PCR reaction system, and the air pressure inside and outside balance PCR reaction unit.In the embodiment of the present invention
In the PCR reaction unit of offer, for realize PCR reaction unit inside and outside gas 51 length of circulation and tubular body setting, first
Through-hole 3 and the second through-hole 4 are arranged in the relative position of semiclosed 2 opposing sidewalls of channel.Since connector 61 is arranged semiclosed
Channel 2 is not provided on the side wall of first through hole 3 and the second through-hole 4, and therefore, the position of connector 61 may be set at two.Together
When, since first through hole 3 and the second through-hole 4 are symmetrical on semiclosed channel 2, and the end face of seal head 52 and extrusion 63
Inclined-plane be it is opposite, therefore, PCR reaction system from PCR reaction pretreating device enter the through-hole of PCR reaction unit according to connection
The position of part 61 sets and sets.As shown in Fig. 1, when the upper surface in semiclosed channel 2 is arranged in connector 61, PCR is anti-
System is answered to enter PCR reaction unit by first through hole 3；When the lower surface in semiclosed channel 2 is arranged in connector 61, PCR
Reaction system enters PCR reaction unit by the second through-hole 4.
First through hole 3 and 4 position of the second through-hole are oppositely arranged so that 51 length of tubular body is logical more than or equal to any one
The position that hole is open to semiclosed channel 2.Further, by first through hole 3 and the setting of the second through-hole 4 apart from semiclosed logical
Road 2 is open closer position, to provide more spaces to PCR reaction system, while can also shorten the length of tubular body 51,
Save material cost.
Attached drawing 3 is please referred to, attached drawing 3 shows the sectional view in attached drawing 1 at A-A.
, it can be seen that PCR reaction tube 1, semiclosed channel 2, tubular body 51 and part seal head 52 are interior from attached drawing 3
Portion's hollow structure, the hollow structure can provide sufficient placement space for PCR reaction system.Provided in an embodiment of the present invention
In PCR reaction unit, the inner cavity in semiclosed channel 2 is open from semiclosed channel 2 to 1 direction of PCR reaction tube in the axial direction
It is gradually reduced.Simultaneously because the shape of tubular body 51 and the inner cavity in semiclosed channel 2 match, thus, semiclosed channel 2 is in axis
Shape on direction is arranged so that tubular body 51 more easily enters in semiclosed channel 2.Further, semiclosed channel 2
Inner cavity is round, oval, diamond shape or rectangular in radial directions, to adapt to different test or detection demand.Further, exist
In the embodiment of the present invention, the inner cavity in semiclosed channel 2 is circle or rectangular in radial directions, and round or rectangular is semiclosed logical
2 inner cavity of road can be convenient for the setting of first through hole 3 and the second through-hole 4.Since the outer shape and size of tubular body 51 are sealed with half
The inner cavity of closed walk 2 matches, thus when the inner cavity in semiclosed channel 2 is round, oval, diamond shape or rectangular in radial directions
When, the shape of tubular body 51 equally mutually should be round, oval, diamond shape or rectangular, with prevent PCR reaction system from tubular body 51 with
Gap outflow or exudation between semiclosed channel 2.
Attached drawing 4 is please referred to, attached drawing 4 shows the stereochemical structure of piston in PCR reaction unit provided in an embodiment of the present invention
Schematic diagram.
In PCR reaction unit provided in an embodiment of the present invention, seal head 52 and tubular body 51 shape in radial directions
It is identical, and the radial dimension of seal head 52 is greater than the radial dimension of tubular body 51.As shown in Fig. 4, tubular body 51 is in radial side
When upward shape is rectangular, the shape of seal head 52 in radial directions is also rectangular.To prevent the work in closing 6 thrust of wedge
Seal head 52 is pushed into semiclosed channel 2 with lower, thus the radial dimension of seal head 52 is greater than the radial dimension of tubular body 51.
The embodiment of the invention also provides the application method of PCR reaction unit, which includes:
PCR reaction unit is before use, the tubular body 51 of piston 5 is put into semiclosed channel 2, and tubular body 51 does not seal
First through hole 3 and the second through-hole 4 are closed, at this point, PCR reaction unit is in semi-closed state.By first through hole 3 or the second through-hole 4
Be connected by pipeline with the pretreating device for being contained with PCR reaction system, be not connected with pipeline another through-hole then with extraneous phase
It is logical.PCR reaction system enters PCR reaction tube 1 from first through hole 3 or the second through-hole 4, and the air in PCR reaction unit is from another
The discharge of one through-hole realizes PCR reaction system in the liquid conveying of this individual section and PCR reaction unit inside and outside air pressure balance.PCR
Reaction system pushes piston 5 into semiclosed channel 2, and then make tubulose completely into pressing closing wedge 6 after PCR reaction tube 1
Body 51 closes first through hole 3 and the second through-hole 4, realizes the closing of PCR reaction unit.
PCR reaction unit provided in an embodiment of the present invention by the way that the tubular body 51 of piston 5 is sent into semiclosed channel 2,
And then the disengaging of tubular body 51 is realized by pressing seal head 52, to realize tubular body 51 to first through hole 3 and the second through-hole 4
Closing and opening so that PCR reaction unit closed process simplify, and then PCR reaction unit be enclosed in manual mode and
It can be realized easily under mechanical system.
It should be noted that, in this document, the relational terms of such as " first " and " second " or the like are used merely to one
A entity or operation with another entity or operate distinguish, without necessarily requiring or implying these entities or operation it
Between there are any actual relationship or orders.Moreover, the terms "include", "comprise" or its any other variant are intended to
Cover non-exclusive inclusion, so that the process, method, article or equipment for including a series of elements not only includes those
Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or setting
Standby intrinsic element.In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that
There is also other identical elements in the process, method, article or apparatus that includes the element.
Those skilled in the art will readily occur to of the invention its after considering specification and the disclosure invented here of practice
Its embodiment.This application is intended to cover any variations, uses, or adaptations of the invention, these modifications, purposes or
Person's adaptive change follows general principle of the invention and including the undocumented common knowledge in the art of the present invention
Or conventional techniques.The description and examples are only to be considered as illustrative, and true scope and spirit of the invention are by following
Claim is pointed out.
It should be understood that the present invention is not limited to the precise structure already described above and shown in the accompanying drawings, and
And various modifications and changes may be made without departing from the scope thereof.The scope of the present invention is limited only by the attached claims.
Claims (8)
1. a kind of PCR reaction unit, which is characterized in that the PCR reaction unit includes the internal PCR reaction tube (1) being connected
With semiclosed channel (2), first through hole (3) and the second through-hole is respectively set on opposite two sidewalls in the semiclosed channel (2)
(4), the inner cavity of the semiclosed channel (2) is open from the semiclosed channel (2) to the PCR reaction tube in the axial direction
(1) direction is gradually reduced；The PCR reaction unit further includes piston (5), and the piston (5) includes tubular body (51) and sealing
Head (52), the inner cavity of the tubular body (51) and the semiclosed channel (2) match, and the end face of the seal head (52) is oblique
Face；The length of the tubular body (51) is greater than the distance between the first through hole (3) and semiclosed channel (2) opening,
The length of the tubular body (51) is greater than the distance between second through-hole (4) and semiclosed channel (2) opening.
2. PCR reaction unit according to claim 1, which is characterized in that the opposite two sidewalls of the semiclosed channel (2)
On the opposite first through hole (3) in position and second through-hole (4) is respectively set.
3. PCR reaction unit according to claim 2, which is characterized in that the inner cavity of the semiclosed channel (2) is in radial direction
It is round, oval, diamond shape or rectangular on direction.
4. PCR reaction unit according to claim 1, which is characterized in that the seal head (52) and the tubular body
(51) shape is identical in radial directions；The radial dimension of the seal head (52) is greater than the radial ruler of the tubular body (51)
It is very little.
5. PCR reaction unit according to claim 1, which is characterized in that the PCR reaction tube (1) is tubulose or flat
Shape reaction tube.
6. PCR reaction unit described in any one of -5 according to claim 1, which is characterized in that the PCR reaction unit is also
Including closing wedge (6), closing wedge (6) includes being sequentially connected the connector (61), pressing piece (62) and extrusion (63) connect,
The semiclosed channel (2), which is not provided on the side wall of the first through hole (3) and second through-hole (4), is arranged the connection
Part (61)；The end face of the extrusion (63) is inclined-plane.
7. PCR reaction unit according to claim 6, which is characterized in that the bevel inclination angle of the extrusion (63)
It is identical as the bevel inclination angle of the seal head (52).
8. a kind of application method of PCR reaction unit, which is characterized in that based on PCR reaction unit described in claim 6 or 7,
The application method includes:
The tubular body of piston is put into semiclosed channel, and the tubular body does not close first through hole and the second through-hole；
The first through hole or second through-hole are connect by pipeline with the device for being contained with PCR reaction system, so that institute
It states PCR reaction system and enters PCR reaction tube from the first through hole or second through-hole；
The PCR reaction system enters pressing closing wedge after the PCR reaction tube, so that tubular body closing described first is logical
Hole and second through-hole.
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| CN201611024477.9A CN106399053B (en) | 2016-11-14 | 2016-11-14 | A kind of PCR reaction unit and its application method |
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| CN108913811B (en) * | 2018-07-05 | 2020-06-16 | 圣湘生物科技股份有限公司 | Rapid amplification method of hepatitis B virus nucleic acid |
| CN113150956A (en) * | 2020-10-30 | 2021-07-23 | 重庆中元汇吉生物技术有限公司 | One-stop reactor and nucleic acid detection analyzer |
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| CN203960207U (en) * | 2014-07-04 | 2014-11-26 | 湖南工业大学 | A kind of container for biological detection |
| CN105695303A (en) * | 2016-03-21 | 2016-06-22 | 北京金诺美生物技术有限公司 | Sample reaction device and reaction method |
| CN106119099A (en) * | 2016-06-14 | 2016-11-16 | 西安交通大学 | A kind of totally-enclosed many targeting nucleic acid isothermal amplification detection integrated apparatus |
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