CN103760330A - Device for collecting and detecting analytes in fluid sample - Google Patents
Device for collecting and detecting analytes in fluid sample Download PDFInfo
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- CN103760330A CN103760330A CN201310755002.7A CN201310755002A CN103760330A CN 103760330 A CN103760330 A CN 103760330A CN 201310755002 A CN201310755002 A CN 201310755002A CN 103760330 A CN103760330 A CN 103760330A
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- piston
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- plunger shaft
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
Abstract
The invention provides a device for collecting and detecting analytes in a fluid sample, which comprises a detection chamber for containing testing elements, a collection chamber for collecting the fluid sample, a piston chamber positioned between the detection chamber and collection chamber, a piston and an opening, wherein the piston has a first position and a second position in the piston chamber; when the piston is in the first position, the piston chamber is communicated with the collection chamber through the fluid at the opening; and in the process of the piston moving to the second position from the first position, the opening is sealed by the piston, and meanwhile, the fluid in the piston chamber is released into the detection chamber. The device can be used for precisely detecting the fluid sample, and can enhance the detection accuracy and operational comfort.
Description
Technical field
The present invention relates to a kind of pick-up unit, the detection of illegal drug in especially a kind of test fluid sample.
Background technology
At present, it is more and more easier that general public obtains violated medicine, and therefore the use of violated medicine is also more and more.In order to guarantee the safety of working environment and the fair competition of physical culture, employee need to detect in enterprise, unit and sports organization, athletic body fluid is for example urinated, and to understand employee and sportsman, whether has used violated medicine.Therefore, it is more and more that Humoral sample Detection and Collect device is used, and need to by layman, be operated in common occasion.For example, fluid sample takes out from container, then drips on chemistry or immunoreagent bar.In such operating process, sample and test all can expose outside, and operator or environment can be polluted or damage.
The device disclosing in existing conventional art, US5 for example, in 119,830, pick-up unit is arranged on the bowl cover of urine cup.When opening the bowl cover of urine cup, cup body can supply the collection of sample and deposit.When needing to detect, first collected sample, then press the parts of a projection on bowl cover, barrier film between pick-up unit and cup body is poked, then cup body is inverted to certain hour, sample can, along the breach being crushed by projection, enter the water suction position of pick-up unit, thereby detect from cup body.
In US6576193B1, disclose another kind of similar pick-up unit, relied on the rotation that is arranged on the piston between collecting chamber and test chamber to come withdrawn fluid sample to detect.Such device is due to piston and piston wall airtight leakage not, in addition owing to relying on the rotation of piston to carry out extracting part shunting body sample, in practical operation, also can produce more inconvenience and safety problem, especially, in rotation process, the fluid being positioned in piston is not easy to flow in test chamber, because under airtight space, it is smooth not that the transfer of fluid sample seems, thereby cause the non-quantitative of fluid sample, and even the amount of fluid sample can not complete detection very little.
In US7270959, also disclosing another fluid sample collects and pick-up unit, the transverse shifting that discloses dependence piston in this patent is realized the sampling of fluid sample, but in practical operation, need user to expend the movement that larger strength promotes piston, also disclose in addition, the piston hand that is used for promoting piston can be separated with piston, and like this in practical operation, piston pushing hands may be lost and cannot complete the movement of piston due to some factor.
In addition, in US Patent No. 6, in 726,879B2, disclosed a kind of urine cup of horizontal edge-on detection, this urine cup comprises three chambeies, one is collecting chamber, one is fluid transfer chamber, and one is test chamber, and two bowl covers, one of them bowl cover is for the sealing of collecting chamber, and another one bowl cover is for detection of whether containing analyte in sample.In this patent, introduce by detecting the direct contacting with fluid sample in sample region of acceptance of reagent strip upstream, realize testing goal.
This is still necessary to provide a kind of new pick-up unit, and the fluid sample that not only can detect needs carries out quantitative sampling, and handled easily, improves the accuracy detecting simultaneously, especially, improves the efficiency detecting and improves quantitative accuracy.
Summary of the invention
On the one hand, the invention provides the device of analyte in a kind of like this collection and test fluid sample, comprising: for holding the test chamber of testing element; For collecting the collecting chamber of fluid sample; Plunger shaft between test chamber and collecting chamber arranges a piston that can move in plunger shaft.In some preferred modes, by the mobile of piston, a part of fluid sample in collecting chamber is transferred in test chamber.
In some preferred modes, in plunger shaft, comprise Yi Ge piston chamber.In some preferred modes, one end of piston and plunger shaft and the wall of plunger shaft form piston chamber, and this piston chamber can hold the segment fluid flow sample from collecting chamber.In other preferred modes, piston chamber comprises the wall of piston, plunger shaft and one end of plunger shaft, and wherein the wall of piston, plunger shaft and one end of plunger shaft surround described piston chamber.
In a preferred mode, piston has the first and second positions in plunger shaft, and when piston is positioned at primary importance, piston chamber is communicated with in fluid by opening with collecting chamber.Like this, segment fluid flow sample is from collecting chamber flows to piston chamber.When piston moves to from primary importance the process of the second place, this opening is sealed, and wherein, when this opening is sealed, the fluid being positioned in piston chamber is released in test chamber.The fluid sample flowing out from piston chamber flows to test chamber, with the analysis and the detection that are arranged in advance testing element in test chamber and have contacted fluid sample analyte.
In some preferred modes, on piston, comprise that one punctures element, when piston moves to the process of the second place from primary importance, described opening is sealed, wherein, when opening is sealed, the element that punctures being positioned in piston chamber pierces through the sealed material that can be punctured, thereby, the fluid being positioned in piston chamber is discharged in test chamber by the opening puncturing on film.In some preferred modes, the film that sealed material is permeable watertight, like this when piston is mobile in plunger shaft, before film is not also punctured, can get rid of gas (if there is) unnecessary in piston chamber, reduce like this resistance that piston moves, thereby more easily promote piston.Preferably, the element that punctures being positioned on piston is positioned at piston chamber.In some modes, piston and puncture element and be connected as a single entity.
Preferably, in above-described embodiment, puncture element and be not positioned at plunger shaft, but film is positioned at outside plunger shaft relatively, so also can arrive the object that punctures film.
In some preferred modes, the sealing-plug that the other end of plunger shaft can be removed seals, on piston, comprise an ejecting structure, the head that for example punctures element is above the shape of non-point, in the process that piston moves in plunger shaft, described in piston seal, when opening, this ejecting structure also can eject sealing-plug from one end of plunger shaft, thereby the segment fluid flow sample in piston chamber is discharged in test chamber.In some preferred modes, described sealing-plug silica gel, rubber, plastic stopper.
In other preferred modes, the sealed material that one end of plunger shaft can be punctured can be diaphragm seal, this film can be aluminium foil or self-adhesive plastic film or plastic sheeting, these films can allow the piston chamber forming in airtight space (except being communicated with collecting chamber fluid by first passage), but these films can easily be punctured.
In other preferred modes, when piston moves to from primary importance the process of the second place, the packed-piston chamber, outer of piston and the opening between collecting chamber, wherein, piston continues to move in piston chamber, and in the process of motion, the outer of piston seals described first passage always.Preferably, the volume of the piston compression piston chamber that continuation is moved in piston chamber, thereby, force the fluid sample in piston chamber to enter in test chamber.Preferably, one or more grooves are set on the outer of piston, in described groove, elastic seal ring are set, this elastic seal ring by the outer of piston and the inwall of piston in sealing.Preferably, in the process of piston outer in sealing first passage, described elastic seal ring is two, and they lay respectively at the both sides of passage.In other preferred modes, the degree of depth essence of the thickness of the outer of piston or length and piston chamber is identical.When piston is in primary importance time, one end of piston is positioned near first passage, and the other end of piston, in plunger shaft, but is not arranged in piston chamber.When piston is in the second place time, near one end of the piston of first passage, be positioned in piston chamber the described passage of outer sealing of piston.Like this, can allow piston fill whole piston chamber, the fluid sample in piston chamber is almost pressed in test chamber substantially.
In some preferred modes, in some modes, the diaphragm seal that one end of plunger shaft can be punctured, sealing film has certain elasticity.When piston is mobile in plunger shaft, especially, when filling flowing full sample body in piston chamber, in the process that piston moves with respect to one end of plunger shaft, force fluid sample moves in piston chamber, in order to reach, can allow piston continue to move to the second place from primary importance, film has certain elasticity, piston moves in the process of the second place like this, the elastic film of the packed-piston chamber other end bloats, now, the element that punctures being positioned at outside piston chamber punctures the elastic film bloating, thereby the fluid sample in piston chamber is discharged into and detects brute force.Certainly, because have the existence of elastic sealing film, once in the time of piston sealed open, can allow the broken element of side puncture film, be also, allow piston continue mobile, allow film bloat, the element that punctures that is arranged on outside punctures.In the time of the face seal opening of the outer of piston, just in sealing, puncture element and puncture film, if piston continues mobile, mobile piston forces the fluid sample in piston chamber all by the film being punctured, to enter test chamber, in this time, the outer of piston still allows opening sealed.During this time, the volume of piston chamber reduces, or even is reduced to zero.
On the other hand, the invention provides the device of analyte in a kind of test fluid sample, comprising: for holding the test chamber of testing element; For collecting the collecting chamber of fluid sample; Plunger shaft between test chamber and collecting chamber; Piston; One opening; Piston has the first and second positions in plunger shaft, and when piston is positioned at primary importance, piston chamber is communicated with in fluid by described opening with collecting chamber; When piston moves to from primary importance the process of the second place, described opening is by piston seal, and wherein, when this opening is by described piston seal, the fluid being positioned in piston chamber is released in test chamber.
Preferably, described plunger shaft is both ends open, and wherein an end opening of plunger shaft is by piston seal, and the sealed material of the other end opening of plunger shaft seals.Preferably, described sealed material is what can be punctured, on described piston, comprise that one punctures element, when piston moves to from primary importance the process of the second place, the opening arranging between piston chamber and collecting chamber is by piston seal, wherein, when this opening is sealed, puncture element and pierce through the sealed material that can be punctured, thereby, the fluid being positioned in piston chamber is discharged in test chamber by thorn sealed material.The sealed material that preferably, can be punctured is ventilative, fluid-tight material.
Preferably, described sealed material is material flexible, that can be punctured, when piston moves to the process of the second place from primary importance, described elastic material is from the other end outwardly convex of plunger shaft, the resilient seal material of projection is punctured element and punctures, thereby, the fluid sample being positioned in piston chamber is discharged in test chamber by the elastic material puncturing.Preferably, described puncture the outside that element is positioned at plunger shaft, and towards described flexible, the material that can be punctured.
Preferably, described plunger shaft is both ends open, and an end opening is wherein by piston seal, and the sealed plug of other end opening seals, on described piston, comprise that one ejects element, when piston moves to from primary importance the process of the second place, the opening arranging between piston chamber and collecting chamber is sealed, wherein, when this opening is sealed, eject element described sealing-plug is ejected, thereby, the fluid being positioned in piston chamber is discharged in test chamber by the one end of being backed down.Preferably, described sealing-plug is silica gel stopper.
In aforementioned all embodiments, preferred, the opening arranging between piston chamber and collecting chamber is sealed by the outer of piston.
In aforementioned all embodiments, preferred, after the opening arranging between piston chamber and collecting chamber is by the sealing of the outer of piston, piston continues to move in plunger shaft, thus the volume of compression piston chamber.
In aforementioned all embodiments, preferably, the length of piston equates with the degree of depth essence of piston chamber, when piston is positioned at primary importance, one end of piston near described piston chamber and the opening between collecting chamber but and this opening of blow-by, the other end of piston is in plunger shaft and away from the opening between described piston chamber and collecting chamber.
In aforementioned all embodiments, preferred, described piston chamber and the width of the opening between collecting chamber are 3-8 millimeter.
In aforementioned all embodiments, preferred, on described piston, there is groove, elastic seal ring is set in groove, thus the inwall of packed-piston and plunger shaft.
In aforementioned all embodiments, preferred, described piston chamber is surrounded by the opening of the wall of piston, part plunger shaft and the described plunger shaft other end.
In aforementioned all embodiments, preferred, in described test chamber, be provided with testing element, fluid sample is urine.
Preferably, in the embodiment of above institute, described first passage or opening are opened on the piston wall of plunger shaft, and the fluid in collecting chamber circulates by first passage and piston chamber's maintenance fluid like this.
Preferably, in above embodiment, piston and a piston push rod are connected as a single entity.By external force, promote push rod, thereby allow piston move in plunger shaft.External force can be manual promotion, can be also to adopt automation structure automatically to promote piston push rod.
Preferably, in the embodiment of above institute, a limit element is set on piston push rod, this element can the initial primary importance of limited piston in plunger shaft, can avoid like this missing push piston and the extraction and the detection that cause liquid.Preferably, limit element is snap ring, and this snap ring can be separated from push rod.
Preferably, in above embodiment, in test chamber, comprise one or more testing elements.Preferably, testing element comprises immunoassay bar and adulterated test-strips.Preferably, the adsorptive pads of testing element is near the bottom of test chamber.
Beneficial effect
By pick-up unit of the present invention, can realize the automatic acquisition of fluid sample, automatically detect and reach the object of sampling accurate quantification, easily operation simultaneously, has reduced the degree of difficulty operating, simultaneously, such device is more easily produced, and in addition, has improved the efficiency detecting.
Accompanying drawing explanation
Fig. 1 is the principle schematic (piston is in primary importance) of one embodiment of the invention;
Fig. 2 is that (piston is in the second place, and sealed open also punctures the other end of plunger shaft for the theory structure schematic diagram of another embodiment of the present invention;
Fig. 3 is the cross-sectional view of another embodiment of the present invention;
Fig. 4 is the principle schematic of another embodiment of the present invention;
Fig. 5 is the principle schematic of one embodiment of the invention;
Fig. 6 is the schematic diagram of piston of the present invention;
Fig. 7 is the cross-sectional view of the pick-up unit in embodiment of the present invention;
Fig. 8 is the cross-sectional view of the pick-up unit in another embodiment of the present invention;
Fig. 9 is the perspective view of the pick-up unit in another embodiment of the present invention;
Figure 10 is the perspective view of the pick-up unit in another embodiment of the present invention.
Description of reference numerals:
Piston 200; Collecting chamber 80; The opening 40 of collecting chamber; Bayonet arrangement 600; Plunger shaft 100; One end 104 of plunger shaft; The other end 101 of plunger shaft; Opening 103 between piston chamber and collecting chamber; The inwall 102 of plunger shaft; Puncture element 300; Piston chamber 70; Piston push rod 400; Push rod lid 500; Piston base 108; Test chamber 10; Testing element 20; Piston outer 201; One end 202 of piston; The other end 205 of piston; Groove 204; 205; The lid 2 of sealing collecting chamber; Sealed material 900.
Describe in detail
Below to relating to structure of the present invention or these technical terms using are described further.
detect
Detect to represent chemical examination or test a kind of material or whether material exists, such as, but be not limited to this, the metabolin of chemical substance, organic compound, mineral compound, metabolism product, medicine or drug metabolite, organic organization or organic organization, nucleic acid, protein or polymkeric substance.In addition, detect the quantity that represents test substances or material.Furtherly, chemical examination also represents immune detection, chemical detection, enzyme detection etc.
sample
The sample of indication of the present invention refers to that those can be used for detecting, chemically examine or diagnose the material that whether has interested analyte.Sample can be, for example, fluid sample, fluid sample can comprise blood, blood plasma, serum, urine, saliva and various juice, can also comprise the fluid solution that solid sample and semi-solid samples form after anticipating.Collect the sample coming and can detect whether there is analyte for methods such as immune detection, chemical detection, enzyme detections.
Sample can be liquid sample, for example liquid sample.Liquid sample comprises body fluid, such as blood, serum, blood plasma, saliva, urine, tears, seminal fluid and marrow; Liquid sample can be also water sample, such as seawater, river, river etc., or from domestic water, municipal water use or service water resource, runoff water or sewage; Sample can be food sample, such as milk and wine.Mucus, semisolid or solid sample can be used for making liquid, eluate, suspending liquid or leachate equal samples.For example, throat or genitals sample may be dipped in and in liquid, make sample.Sample can comprise potpourri or any relevant potpourri of liquid, solid and gas, such as the cell suspending liquid in dilution or solution.Sample comprises biological substance, such as cell, microorganism, organelle and biochemical complexes.Liquid sample can be produced from solid, semisolid or high-viscosity materials such as soil, ight soil, tissue, organ, biological fluid or other occurring in nature non-liquid samples.For example, the solution that these solids or semi-solid samples can be suitable with dilution one class mixes.Sample can be macerated, freezing and thaw, or other extracting method form liquid samples.Remaining particulate material can be used the method that filtration or precipitation etc. are traditional to remove.
analyte
By apparatus and method of the present invention, can analyze any analyte.Analyte can detect in any fluid or liquefied sample, urine for example, saliva, saliva, blood, blood plasma, or serum.
The analyte that the present invention refers to can be " drug abuse " (DOA) or other there is interested material in sample." drug abuse " (DOA) refers to that non-medical destination used medicine (conventionally plaing a part to benumb neural).Abuse these medicines and can cause body & mind to suffer damage, produce dependence, addicted and/or dead.The example of drug abuse comprises cocaine; Amphetamine (for example, black beauty, white amphetamine tablet, dextroamphetamine, dexie, Beans); Crystal methamphetamine (crank, meth, crystal, speed); Barbiturate (as Valium, Roche Pharmaceuticals, Nutley, New Jersey); Sedative (paramedicines of sleeping); Lysergic acid diethylamide (LSD); Inhibitor (downers, goofballs, barbs, blue devils, yellow jackets, methaqualone); The anti-antidepressant of tricyclic antidepressants (TCA, i.e. imipramine, amitriptyline and doxepin); Hog (PCP); Tetrahydrocannabinol (THC, pot, dope, hash, weed, etc.), opiate (being morphine, opium, codeine, heroin, hydroxyl dihydrocodeinone), anxiolytic and hypnotic sedative agent, anxiolytic is that a class is mainly used in anxiety reduction, nervous, frightened, set the mind at rest, have the medicine of hypnosis sedation concurrently, comprise Benzodiazepines (benzodiazepines, BZ), atypia BZ class, merge phenodiazine NB23C class, the tall and erect class of benzene nitrogen, the part class of BZ acceptor, open loop BZ class, diphenylmethane derivatives, piperazine carboxylic acid salt, piperidine carboxylic acid salt, Kui azoles quinoline ketone, thiazine and thiazole, other heterocyclic, imidazole type calmness/anodyne, propanediol derivative-carbamates, fatty compound, anthracene derivative etc.Use this device also can be for detection of belonging to medical usage but the easily detection of overdose, as tricyclic antidepressant (imipramine or analog) and Paracetamol etc.After these medicines are absorbed by the body, can resolve into different small-molecule substances, these small-molecule substances are present in the body fluid such as blood, urine, saliva, sweat or part body fluid exists above-mentioned small-molecule substance.
testing element
Device of the present invention can adopt multiple test-strips, and this is according to the object of being disinclined to analyte to be checked and test.Test-strips can roughly be divided into two classes, detects test-strips and anti-adulterated test-strips.Detect test-strips and comprise the material for detection of composition to be checked in sample.Anti-adulterated test-strips comprises the material for detection of sample material characteristic.The test-strips of two types all comprises sample application zone and detection zone.
detect test-strips: multiple detection test-strips can be used in the present invention.Detect the test that test-strips comprises analyte in immune detection or chemical detection and detection sample.These analytes comprise: the metabolin of drug abuse or hint health status.
Above-described " drug abuse " (DOA) material for example.For the legal medication of medical object, but incidental dosage is excessive, also can adopt test-strips of the present invention to detect.These medicines comprise: three anti-strongly fragrant dose of rings (imipramine and its analog) and containing the OTC product of acetaminophen (such as being positioned at the medicine of the trade mark acenol by name that the washingtonian McNeil-PPC of Pennsylvania, America company provides).This detection can help patient that emergency rescue personnel judges stupor whether by accident excessive use a kind of in these medicines.
Can imply that the metabolin in the urine of health status includes but not limited to creatinine, cholerythrin, nitrite, protein (non-specific), hormone is (as human chorionic gonadotrophin, corpus luteum hormone, follicular stimulating hormone etc.), blood, leucocyte, sugar, heavy metal or toxin, bacterium composition is (as the specific protein of Special Category bacterium or carbohydrate, such as colon bacillus 0157: H7, staphylococcus, salmonella, fusobacterium, Campylobacter, monocytosis kytoplasm base (L.monocytogenes), vibrio, or cactus bacillus) and physical property, as pH value and proportion.Any other Clinical Urinary liquid chemical analyte that can adopt fast detecting mode can be applied in this device.
Detect test-strips and can have various ways.Generally speaking, test-strips is comprised of absorbent material.This absorbent material comprises sample application zone, raw material district and test results zone.Sample is added to sample application zone and by capillary force wet raw material district.In raw material district, sample dissolves and mixes those and detect the necessary raw material of analysans (if existing in sample).The sample that has mixed raw material continues moistening test result zone.Extra raw material is fixed on test result zone.One of first group of raw material in these raw materials and analysans (if present) or raw material district reacts.If sample exists analyte really, will there is a signal in test section, otherwise there will not be signal.
As an example more specifically, the detection test-strips that the data of following United States Patent (USP) or Patent Application Publication discloses can be used in the present invention: 5, 252, 496, 5, 415, 994, 5, 559, 041, 5, 602, 040, 5, 656, 503, 5, 712, 170, 5, 877, 028, 5, 965, 458, 6, 046, 058, 6, 136, 610, 6, 140, 136, 6, 183, 972, 6, 187, 268, 6, 187, 598, 6, 194, 221, 6, 194, 224, 6, 221, 678, 6, 228, 660, 6, 241, 689, 6, 248, 598, 6, 271, 046, 6, 297, 020, 6, 316, 205, 6, 372, 514, 6, 338, 969, 6, 368, 873, 6, 372, 516, 6, 375, 896, 6, 375, 897, 6, 391, 652, 6, 403, 383, 6, 418, 606, 6, 429, 026, 6, 464, 939, 6, 468, 474, 6, 485, 982, 6, 506, 612, 6, 514, 769, 6, 528, 323, 6, 548, 019, 6, 730, 268, 2001/0004532, 2001/0021536, 2001/0023076, 2002/0001854, 2002/0004019, 2002/0031840, 2002/0031845, 2002/0052050, 2002/0085953, 2002/0137231, 2002/0173047, 2002/0132267, 2003/0129673, 2003/0207466, 2004/0018636 and 2004/0191760.
anti-adulterated test-strips: anti-adulterated test-strips for example, for detection of sample material characteristic or pollution: temperature, proportion, pH value, oxygenant pollution, glutaconaldehyde pollution, nitrite pollution, ascorbic acid usp/bp pollute and creatinine pollutes.Test purpose for this reason, it is desirable adopting following test-strips.This test-strips has merged those and has defeated for detection of extra chemical substance or other intention the reagent that detects and conceal drug taking behavior.These test-strips can be used in any form, for example immune detection or chemical detection.In some embodiments, in this test-strips, can comprise whether the chemical examination of creatinine and protein was diluted to detect urine sample.Anti-adulterated test-strips also can detect Cobastab or other intention is defeated detection and joined the material in urine.These materials comprise: the commodity that the commodity that glutaraldehyde, nitrite, chromate, vinegar, trade mark are Visine (Pfizer by New York city,U.S provides), sodium bicarbonate, trade mark are Drano (the S.C.Johnson company by the Racine city of Wisconsin, USA provides), soft drink, oxygenant (for example bleaching agent, hydrogen peroxide, pyridine, chromium chloride hydrochlorate).
The test-strips of test liquid samples substance characteristics can be used together with the present invention.These test-strips comprise for transmit the absorbent material of liquid sample by test-strips, with the filtering element of absorbent material phase fluid connection, with the raw material pad of filtering element phase fluid connection and one transparency cover optionally.Raw material pad includes the raw material for generation of the symbol that can detect, and transparent plate (if employed) observation relevant to sample properties and by transparency cover and this device of this symbol obtains.Described filtering element can be manufactured by the material can suppression fluid refluxing from raw material pad to absorbent material." suppress reflux " refers to that the Fluid Volume that flows to absorbent material from raw material pad seldom and not can change assay or seldom detect and out or not can not make this testing result become indefinite from the amount that raw material pad is transferred to the raw material of absorbent material or adjacent raw material pad.Test-strips also can comprise and is placed in a part for filtering material and the material that do not get wet between absorbent material.This material that do not get wet is to absorbent material, to reflux from raw material pad in order to suppress sample.Liquid sample transmits through whole test-strips because of capillary action." capillary action " refers to that the inside by liquid and wall or material that the art personnel know interacts and the physical influence of generation, and this physical influence can cause liquid to flow through this material." absorbent material " refers to that the material of easy attraction or absorption fluids and this material are suitable for capillary action fluid is transferred to another place by the somewhere of this material." filtering element " promotes the even diffusion of sample during to raw material pad in sample flow.Thereby promote successively on raw material pad to form the symbol that can detect uniformly.Filtering element also suppresses sample and is refluxed to absorbent material by raw material pad, thereby the chemical substance that also suppresses raw material is transferred to another raw material pad by a raw material pad." material does not get wet " refers to the material that the fluid of the quantity that does not allow meeting change testing result passes.The normally mobile barrier of fluid of material does not get wet.
The adulterated test-strips of capillary flow of other type also can be integrated in device of the present invention.For example, the adulterated test-strips disclosing in following listed reference can be applied in the present invention: 2002/0001845,2002/0098512,2002/0155028,2003/0045003,5,922,283,6,248,598,6,514,768,6,537,823 and 6,548,019.
piston and plunger shaft
In some concrete embodiments, plunger shaft 100 can be a cylindrical shape, in the middle of right cylinder, is the structure of hollow, the cavity that the part of hollow is plunger shaft.And the two ends of plunger shaft can be the form of opening.Piston 200 is inserted into plunger shaft from the opening 104 of one end of plunger shaft, and the outer 201 of piston contacts and the one end 104 in packed-piston chamber with the inwall 102 of plunger shaft, allows the fluid sample that enters plunger shaft not be leaked to outside plunger shaft.In another mode, on the outer 201 of piston, have annular groove 204,203, elastic seal ring 501 is set on groove, this elastic seal ring contacts with the inwall 102 of plunger shaft and reaches the effect of sealing, the number of elastic seal ring and the number of groove can be 1, and 2 or a plurality of, they can divide and be arranged on piston, when piston is enough thick, groove can be arranged on the two ends 202,205 of piston outer, and an O-ring seal 501 is installed respectively in groove.Such sealing allows the fluid sample entering in plunger shaft at least can between the inwall 102 of piston outer 201 and plunger shaft, not leak.Except such sealing means, between piston 200 and plunger shaft 100, also can adopt its her forms such as latch, buckle and seal.
In some modes, in order to allow piston chamber 70 naturally obtain swimmingly the segment fluid flow sample in collecting chamber, on the wall of plunger shaft, have opening 103 or passage, allow collecting chamber 80Yu piston chamber 70 be communicated with in fluid.Like this, when having in collecting chamber or collecting fluid sample, fluid sample enters into piston chamber by opening or passage naturally, or fills full whole piston chamber.For quickly and accurately transferring to 10 li of test chamber from the fluid sample of 80 li of collecting chambers, piston 200 is in original state, piston be generally positioned at opening 103 near, or semitight opening or passage are collected when having fluid sample, like this because opening 103He piston chamber 70 phase fluids are communicated with in collecting chamber, fluid sample in collecting chamber flows in piston chamber naturally, in this time, piston is in primary importance (Fig. 1, Fig. 4).Due to the diaphragm seal that can be punctured, double faced adhesive tape or other diaphragm seals piston chamber 70 other end 108(during this time, a part of space that plunger shaft is 100 li is just restricted to the space of piston chamber 70, and the other end 101 of plunger shaft just becomes the bottom 108 of piston chamber 70 naturally).On piston 200, arrange one and puncture element 300, this punctures the encapsulant 900 that element can puncture the packed-piston chamber other end.In some modes, this punctures element 300 and is positioned at 70 li of piston chamber and is positioned on piston 200, and, allow one end 301(puncturing that punctures element puncture in other words sharp keen tartar) and the other end 101 of seal material 900 or plunger shaft between distance be less than or equal to the length of the opening on plunger shaft wall, when piston 200 100 li of plunger shaft in the other end 101 motions of plunger shaft, when once outer 201 essence of piston seal this opening 103 completely, puncture the encapsulant 900 that element 300 also punctures packed-piston chamber 100 other ends 101 simultaneously, like this, the liquid sample that is positioned at 70 li of piston chamber enters in test chamber by the sealed material puncturing immediately, the fluid sample that enters 10 li of test chamber can contact with testing element 20.Like this, opening 103 sealed and puncture sealed material 900 almost essence at synchronization, complete.Here said " essence " sealed open 103 refers to that the outer of piston seals opening, avoids the fluid sample of 80 li of collecting chambers to enter into 70 li of piston chamber again.Generally, in promoting the process of piston, because velocity ratio is very fast, almost can being completed at synchronization by step and the step that punctures sealed material of opening, this has just improved efficiency, particularly by hand when operation, has increased the comfortableness of operation.
In another preferred mode, the sealed material 900 of one end, packed-piston chamber is ventilative, waterproof material.In other modes, this is ventilative, waterproof material is also the material being easily punctured.
In some other examples, the sealed material 900 of the one end 101 in packed-piston chamber is not the sealing film easily puncturing, but a stopper is for example silica gel stopper.In the time of piston 200 piston chamber 70 or 100 li of motions of plunger shaft, at the extension 201 sealed open 103(piston chamber of piston 100 and opening or the passage between collecting chamber) in, be arranged on piston 100 and be positioned at push rod (puncturing slope element does not during this time have sharp keen tip and become non-sharp keen top) in piston chamber also along with travelling forward, this push rod ejects the stopper of 70 bottoms 108, packed-piston chamber on (stopper that is also one end, packed-piston chamber), thereby the fluid sample of making way in piston chamber enters in test chamber.Adopt the effect of stopper to be better than the process that punctures sealed material, because after stopper is ejected, the bottom of whole piston chamber is almost opened, fluid sample more easily flows in test chamber by the opening of making.And puncture element, be generally punctured before this osculum, and then tear larger openning (with respect to the situation of plug seal), fluid is not easy to flow out from piston chamber.
In another preferred mode, when piston 200 is in 100 li of motions of plunger shaft time, the volume of piston chamber 70 can be compressed.(Fig. 8 when the sealed material of the other end in packed-piston chamber is resilient material, wherein dotted line position is the state that there is no resilient material compressed time), the motion of piston can allow these resilient materials 90 to outer process, for example the volume in compression piston chamber (in concrete mode as above).In these examples of implementation, puncturing element 300 not necessarily needs to be positioned in piston chamber, but be positioned at (Fig. 8) outside piston chamber, when piston is positioned at initial position, the broken tip 301 of the side of the broken element of side is near seal element, but can not puncture seal element, and this in time, flexible member can overcome the pressure of liquid and produce the projection under micro-, but such projection not essence be punctured.In the time of 70 li of motions of piston 200 piston chamber, once in the time of the 201 packed-piston chambers 70, outer of piston and the opening between collecting chamber 80 103, the moment that this opening is sealed, whole piston chamber is in airtight situation, piston is moving forward a little a little, 1-2 millimeter for example, also under the inertia effect of piston movement, volume due to the compressing piston chamber 70 of piston 200, resilient material is owing to being subject to the exterior protrusions (Fig. 8) of the compressing Er Xiang piston chamber of external force, once the part of projection is encountered the broken summit of side (the sharp tip also puncturing in time) that punctures element, this resilient material is punctured, thereby discharge fluid sample in piston chamber in test chamber.This resilient material is also the material being easily punctured, such as very thin rubber film etc.
In above embodiment, preferred, in order to allow fluid sample all flow into 10 li of test chambers from piston chamber 70, contact with the testing element 20 in test chamber, guarantee that fluid sample in each final device that becomes to produce enters the volume of test chamber the same, allow the thickness h of piston 200
*(length on outer 201 edges of piston in other words) essence is consistent with length or the degree of depth h of piston chamber 70, like this, when piston 200 is positioned at initial primary importance, between one end 205 piston chamber 70 of piston outer and collecting chamber 80 opening 103 neighbouring (Fig. 4), or piston outer semitight opening, fluid sample in collecting chamber can arrive in piston chamber smoothly like this, for example, if having fluid sample, urine in collecting chamber.When piston is positioned at primary importance, whole piston 200 is positioned at a side of opening and is positioned at 100 li of plunger shaft; When being pushed along with piston, piston outer 201 is once sealed open 103, be positioned at the sealed material 900 that element 300 has also punctured packed-piston chamber 100 other ends 101 simultaneously that punctures in piston chamber this time, or be positioned at the sealing lid (silica gel cover) that push rod in piston chamber is pushed the packed-piston chamber other end 101 open, this time, the fluid sample that is positioned at 70 li of piston chamber enters in test chamber, also just at the same time, the fluid sample that collecting chamber is 80 li can not enter into 70 li of piston chamber again.Along with piston continues to move in piston chamber, in fact, the volume of piston chamber is reduced gradually, piston 200 can all be pressed in test chamber all liq of 70 li of piston chamber, in compression piston chamber 70, the outer 201 of piston is the opening 103 that sealing communicates with piston chamber 70 always, and in this time, the liquid sample in collecting chamber just can not flow into 70 li of piston chamber (Fig. 5) again.For allow piston 200 can be in motion process sealed open 103 all the time, can on the outer 201 of piston, open one or two groove 204,205, elastic seal ring 501 is set in groove, 502, when piston 200 moves to the bottom of piston chamber 70 (the namely other end 101 of plunger shaft), the sealed elastic element 501 on being separately positioned on piston and being, 502 are positioned at the both sides of opening 103, have increased like this effect of piston seal opening.
The such design of the present invention is compared with traditional similar designs, can reach the more object of accurate quantification.For example, volume in the piston chamber 216 of 7270958 li of descriptions of United States Patent (USP) is fixed, when piston chamber and collecting chamber 208 openings sealed after, piston chamber's integral body moves to next position, although when arriving next position, partially liq sample in piston chamber can enter test chamber 300, but the amount entering is inaccurate, because test chamber is also sometimes the state (preventing from entering outside the leak of liquid auto levelizer of test chamber) of almost sealing, so the liquid being positioned in piston chamber need to overcome the pressure in test chamber and can naturally all not enter into test chamber.But the present invention, because the volume of piston compressing piston chamber almost can be pressed into the fluid sample in whole piston chamber in test chamber, enters so the present invention has carried out this to traditional design, makes the amount of sampling more accurate.And the present invention not only can accurate quantification, and, allow piston can move fast in plunger shaft, reduced the friction force of piston and plunger shaft, make operation more laborsaving.In addition, traditional piston chamber is mass motion, need the inwall of packed-piston and plunger shaft more, guarantee liquid is not leaked out, in order to guarantee that liquid is not leaked, must take to increase the acting force of piston and plunger shaft inwall, this has just increased the acting force that promotes piston virtually, has increased the difficulty of operation.In actual product is used, when operating personnel need a plurality of device of continued operation, each only need to apply less strength seems the most important.The present invention from the defect of traditional design, adopts different mentalities of designing just completely, has reduced the acting force being applied on piston, has also increased the efficiency of operation and the accuracy of sample size simultaneously.
In other preferred modes, in opening 103 or passage between design collecting chamber and piston chamber, the size of opening is also a very important factor, if opening design is too little, due to capillary effect, the fluid entering in collecting chamber is not easy naturally to flow to smoothly piston chamber, sometimes occurs not being full of the situation of piston chamber, or is appeared in piston chamber by drum.If too large, the outer of piston is not easy sealing.Through our experiment, find that the width of the opening of piston is that 5-8 millimeter is best.
For allow piston in stable in primary importance, and not easily in 70 li of motions of piston chamber or mobile, a push rod 400 is set on one end 202 of piston, this push rod and piston are connected as a single entity, a limit fastener 600 is set on push rod, by limit fastener 600 can allow piston stable in initial primary importance, allow the stable state in opening of opening 103.Preferably, on piston push rod, arrange and push away cap 500, the width that pushes away cap is greater than the diameter of piston.When operation time, remove buckle 600, by promotion, push away cap 500 and allow piston move in plunger shaft, wait and move to after suitable distance, owing to pushing away the diameter of cap and be greater than the diameter of piston, push away on the opening of one end that cap can only be pushed over plunger shaft.Like this, the distance of piston in plunger shaft is by piston rod and pushed away the concrete restriction of cap (Fig. 1-3).
In some preferred modes, piston, piston push rod, push away cap and puncture element (or push rod) and can pass through the design disposal molding of mould.
collecting chamber, test chamber and plunger shaft
Collecting chamber 80 is for collecting the cavity body structure of fluid sample, collecting chamber can with the public wall of plunger shaft, or plunger shaft be positioned at collecting chamber bottom below, by opening 103, be communicated with collecting chamber maintenance fluid.Collecting chamber 80 has opening, can have lid and seal collecting chamber on opening.The volume of collecting chamber can be set arbitrarily, for example, be 100-500 milliliter.Test chamber 10 is positioned at the outside of collecting chamber, in test chamber, testing element 20 can be set.The position of collecting chamber of the present invention and plunger shaft and test chamber arrange can with traditional arrange the same, the setting position of for example describing in United States Patent (USP) 6576193 and 7270959 can be used as embodiments more of the present invention to carry out, and the material that also comprises these component processing is also the plastic material preparation that this area is known.The setting of test chamber and collecting chamber and testing element also can be with reference to the specific descriptions of 7270959 li of United States Patent (USP)s, as a part of the present invention.In the time of in testing element is arranged on test chamber, test chamber can be for transparent, like this can be by the test result on transparent test chamber read test bar.
In a preferred mode, Fig. 7 for example, this device comprises collection chamber 80, and collecting chamber has opening 40, and this opening can be sealed by lid 2.The form of sealing of lid opening can make helicitic texture, also the form of latch.In the bottom of collecting chamber, have opening 13, opening is communicated with in fluid with plunger shaft, at opening of the other end 103 opening of plunger shaft 100, and sealed material 900 sealings of this opening, the material of sealing material for being punctured, such as thinking film, aluminium foil, double faced adhesive tape etc.On piston 200, have be connected as a single entity puncture element 300.Outside at the plunger shaft other end 103 is test chamber, is provided with testing element in test chamber.On piston, be provided with groove, in groove, be provided with elastic seal ring 501,502.Piston 200 is arranged on one end of plunger shaft 104.When piston is at initial position time, with the one end that punctures element, be positioned at 70 li of piston chamber, but sealed open 103 not, now, if there is fluid sample to be collected in 80 li of collecting chambers, fluid sample, due to Action of Gravity Field, flows in piston chamber naturally.This time, when needs detect, take off the buckle 600 setting in advance on piston rod, promote piston 200 to the other end 101 motions of plunger shaft, in sealed open 103, puncture element and puncture sealed material 900, this time, because opening is sealed, the liquid sample being positioned in collecting chamber is not flowing into piston chamber, meanwhile, owing to puncturing element, has punctured sealed material 900, be positioned in piston chamber fluid sample and flow into test chamber and contact with testing element, complete the detection of analyte in fluid sample.In order better to allow the fluid sample in piston chamber enter test chamber, moving in the follow-up reforwarding of piston seal opening 103, the volume of compression piston chamber, forces the whole fluid samples that are arranged in piston chamber to enter into test chamber completely, completes the accurate quantification of fluid sample.
In another mode, Fig. 8 for example, this device comprises collection chamber 80, and collecting chamber has opening 40, and this opening can be sealed by lid 2.The form of sealing of lid opening can make helicitic texture, also the form of latch.In the bottom of collecting chamber, have opening 13, opening is communicated with in fluid with plunger shaft, at opening of the other end 103 opening of plunger shaft 100, and sealed material 900 sealings of this opening, sealing material has elasticity and can be punctured, for example rubber film, Emulsoid-film, plastic sheeting.In the outside of the plunger shaft other end 103, over against the opposite of sealed material, arrange and puncture element, now puncture element and be not positioned in plunger shaft, but be positioned at plunger shaft outside.The outside of the plunger shaft other end 103 is test chamber, is provided with testing element in test chamber.On piston, be provided with groove, in groove, be provided with elastic seal ring 501,502.Piston 200 is arranged on one end of plunger shaft 104.When piston is at initial position time, puncture element near sealed material, but do not contact sealed material, now, but piston is positioned at a side of opening 103 sealed open 103 not, now, if there is fluid sample to be collected in 80 li of collecting chambers, fluid sample, due to Action of Gravity Field, flows in piston chamber naturally.This time, when needs detect, take off the buckle 600 setting in advance on piston rod, promote piston 200 to the other end 101 motions of plunger shaft, in sealed open 103, puncture element and puncture sealed material 900, this time, because opening is sealed, the liquid sample being positioned in collecting chamber no longer flow into piston chamber, simultaneously, owing to puncturing element, punctured sealed material 900, being positioned in piston chamber fluid sample flow into test chamber and contacts with testing element, complete the detection of analyte in fluid sample, because in the time of piston seal opening, because piston continues motion in piston chamber, in Shi Rang piston chamber, air pressure increases in fact, the air pressure increasing forces to be had flexible sealing film and bulges, once the contact of the surface of the elastic film bloating punctures element, just being punctured element punctures, discharge fluid sample in test chamber.In order better to allow the fluid sample in piston chamber enter test chamber, moving in the follow-up reforwarding of piston seal opening 103, the volume of compression piston chamber, forces the whole fluid samples that are arranged in piston chamber to enter into test chamber completely, completes the accurate quantification of fluid sample.
In another embodiment, as shown in Figure 9, other the embodiment with shown in Fig. 7 is identical, difference is: the piston push rod 400 being connected as a single entity is set on piston, on piston push rod, arrange and push away cap 500, plunger shaft 100 is positioned at the bottom of collecting chamber, and on piston wall, open an opening 103, the width of opening is 3-8 mm wide, simultaneously, buckle structure 600 is set on piston push rod, and buckle structure limited piston is stably in initial primary importance in plunger shaft, that is: blow-by opening 103.When needs operate, remove buckle structure 600, push away cap 500, thereby allow piston move in plunger shaft, in piston seal opening, piston forces the fluid sample in plunger shaft to enter in test chamber.Enter the mode of test chamber a kind of the carrying out in can the concrete mode shown in Fig. 1-8, for example puncture element and puncture sealed material or eject element and eject sealing cap or allow sealed material there is elasticity, allow be arranged on piston chamber outside puncture element side slope flexible member etc.Certainly, also can make is in order better to allow the fluid sample in piston chamber enter test chamber, moving in the follow-up reforwarding of piston seal opening 103, the volume of compression piston chamber, force the whole fluid samples that are arranged in piston chamber to enter into test chamber completely, complete the accurate quantification of fluid sample.
In another mode, Figure 10 for example, other the embodiment with shown in Fig. 7 is identical, difference is: one end 101 openings of plunger shaft, a sealing plug (being for example silica gel stopper) is set on opening and seals up this opening, a bar is set on piston, motion along with piston, in 201 sealed opens 103 of piston outer, push rod ejects stopper in test chamber, the fluid sample being positioned in piston chamber is discharged in test chamber simultaneously, has contacted the detection of analyte with testing element.In order better to allow the fluid sample in piston chamber enter test chamber, moving in the follow-up reforwarding of piston seal opening 103, the volume of compression piston chamber, forces the whole fluid samples that are arranged in piston chamber to enter into test chamber completely, completes the accurate quantification of fluid sample.
Embodiment
By reference to the accompanying drawings the specific embodiment of the present invention is described in detail now, this explanation is not that the claims in the present invention are carried out to any restriction, but is used for illustrating how to realize the present invention, belongs to a concrete mode of the present invention.
Specific embodiment
According to the disclosed method of the patent of US7270959, manufacture the product of 1000, manufacture 1000 products of device of the present invention simultaneously.The collecting chamber volume (450 milliliters) of product that US7270959 patent (contrast) is described is identical with the product of apparatus of the present invention with the volume (25 milliliters) of containing fluid sample in plunger shaft, in addition, collecting chamber is the same with size and the position of the opening of plunger shaft, and the volume of plunger shaft too.In each product, fill water or the urine specimen of 300 milliliters, method according to each self-described operates, contrast United States Patent (USP) (US7270959) allows piston have three kinds of states, and the design of piston of the present invention is different from the design of the piston of contrast US Patent No. 7270959, mode of operation also occurs that essence is different.Meanwhile, relatively transfer to the difference of the volume of the sample in test chamber, the time difference of compare operation simultaneously.
The comparison of the device comparative parameter of table 1 device of the present invention and contrast 1.
| ? | Leakage ratio | Arrive the urine liquid volume of test chamber | Arrive the water volume of test chamber | Arrive the liquid volume of piston chamber | |
| Contrast | |||||
| 20/1000 | 15.2 milliliters (on average) | 14.3 milliliters (on average) | 23.2 milliliters (on average) | 2.3 second | |
| The present invention | 12/1000 | 24.3 milliliters (on average) | 23.8 milliliters (on average) | 24.8 milliliters (on average) | 1.5 seconds. |
Table 2 device of the present invention compares with exerting oneself with the device comfort level of contrast 1.
| ? | Exerting oneself of push piston | Comfort level |
| Contrast | 200.4 newton | 200(10 divides); 300(8 divides); 400(6 divides); 100(3 divides) |
| The present invention | 120.45 newton | 400(10 divides); 500(8 divides); 80(6 divides); 20(3 divides) |
Passed through more than 1000 common operator and operated, comfort level is graded, grading is that standard is as follows: 10 are divided into the most comfortable, 8 be divided into comfortable; 6 are divided into generally; 3 be divided into uncomfortable.
Claims (13)
1. a device for analyte in collection and test fluid sample, comprising: for holding the test chamber of testing element; For collecting the collecting chamber of fluid sample; Plunger shaft between test chamber and collecting chamber; Piston; Be arranged on the opening arranging between piston chamber and collecting chamber; Piston has the first and second positions in plunger shaft, and when piston is positioned at primary importance, piston chamber is communicated with in fluid by described opening with collecting chamber; When piston moves to from primary importance the process of the second place, described opening is by piston seal, and wherein, when this opening is by described piston seal, the fluid being positioned in piston chamber is released in test chamber.
2. device according to claim 1, described plunger shaft comprises the two ends of opening, wherein, an end opening of plunger shaft is by piston seal, the sealed material sealing of the other end opening of plunger shaft.
3. device according to claim 2, described sealed material is what can be punctured, on described piston, comprise that one punctures element, when piston moves to from described primary importance the process of the described second place, the opening arranging between piston chamber and collecting chamber is by piston seal, wherein, when this opening is sealed, puncture element and pierce through the sealed material that can be punctured, thereby, the fluid being positioned in piston chamber is discharged in test chamber by the sealed material puncturing.
4. device according to claim 3, the sealed material that can be punctured is ventilative, fluid-tight material.
5. device according to claim 2, described sealed material is material flexible, that can be punctured, when piston moves to the process of the second place from primary importance, described elastic material is from the other end outwardly convex of plunger shaft, the resilient seal material of projection is punctured element and is punctured simultaneously, thereby, the fluid sample being positioned in piston chamber is discharged in test chamber by the elastic material puncturing.
6. device according to claim 5, wherein, described punctures the outside that element is positioned at plunger shaft, and towards described flexible, the material that can be punctured, only has in the time of elastic protrusion, punctures element and just punctures described resilient seal material.
7. device according to claim 1, described plunger shaft is both ends open, an end opening is wherein by piston seal, the sealed plug sealing of other end opening, on described piston, comprise that one ejects element, when piston moves to the process of the second place from primary importance, the opening arranging between piston chamber and collecting chamber is sealed, wherein, when this opening is sealed, eject element described sealing-plug is ejected, thereby, the fluid being positioned in piston chamber is discharged in test chamber by the one end of being backed down.
8. device according to claim 7, wherein, described sealing-plug is silica gel stopper.
9. according to the device one of claim 1-8 Suo Shu, wherein, the opening arranging between piston chamber and collecting chamber is sealed by the outer of piston.
10. according to the device one of claim 1-8 Suo Shu, wherein, after the opening arranging between piston chamber and collecting chamber is by the sealing of the outer of piston, piston continues to move in plunger shaft, thus the volume of compression piston chamber.
11. according to the device one of claim 1-8 Suo Shu, wherein, the length of piston equates with the degree of depth essence of piston chamber, when piston is positioned at primary importance, one end of piston near described piston chamber and the opening between collecting chamber but and this opening of blow-by, the other end of piston is in plunger shaft and away from the opening between described piston chamber and collecting chamber.
12. according to the device one of claim 1-8 Suo Shu, and wherein, described piston chamber and the width of the opening between collecting chamber are 3-8 millimeter.
13. according to the device one of claim 1-8 Suo Shu, and wherein, described piston chamber is surrounded by the opening of the wall of one end of piston, part plunger shaft and the described plunger shaft other end.
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| CN201310755002.7A CN103760330B (en) | 2013-12-31 | 2013-12-31 | A kind ofly to collect and the device of analyte in test fluid sample |
| US14/318,489 US9352313B2 (en) | 2013-12-31 | 2014-06-27 | Device for collecting and testing analyte in a liquid sample |
| EP14200592.5A EP2889086B1 (en) | 2013-12-31 | 2014-12-30 | A device for collecting and testing analyte in a liquid sample |
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| CN106399053B (en) * | 2016-11-14 | 2019-07-05 | 湖南圣湘生物科技有限公司 | A kind of PCR reaction unit and its application method |
| CN106841586A (en) * | 2017-01-22 | 2017-06-13 | 英科新创(厦门)科技有限公司 | A kind of integration chromatography detection means |
| CN106841587A (en) * | 2017-01-22 | 2017-06-13 | 英科新创(厦门)科技有限公司 | A kind of integration chromatography detection means |
| CN107576809A (en) * | 2017-09-30 | 2018-01-12 | 江苏宝卫士生物科技有限公司 | A kind of real-time test device |
| CN107817358A (en) * | 2017-12-14 | 2018-03-20 | 江苏宝卫士生物科技有限公司 | A kind of diagnostic reagent detection means and its method |
| CN107817358B (en) * | 2017-12-14 | 2023-11-07 | 江苏宝卫士生物科技有限公司 | Diagnostic reagent detection device and method thereof |
| CN110567748A (en) * | 2019-07-25 | 2019-12-13 | 杭州迪图科技有限公司 | Device for sampling |
| CN110567748B (en) * | 2019-07-25 | 2022-07-01 | 杭州迪图科技有限公司 | Device for sampling |
| CN110907371A (en) * | 2019-11-26 | 2020-03-24 | 中国地质调查局水文地质环境地质调查中心 | On-site detection reagent tube for ferric ions in water and detection method |
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