CN107290515A - High flux immunoassay device - Google Patents
High flux immunoassay device Download PDFInfo
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- CN107290515A CN107290515A CN201610222107.XA CN201610222107A CN107290515A CN 107290515 A CN107290515 A CN 107290515A CN 201610222107 A CN201610222107 A CN 201610222107A CN 107290515 A CN107290515 A CN 107290515A
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- 238000003018 immunoassay Methods 0.000 title claims abstract description 19
- 230000004907 flux Effects 0.000 title claims abstract description 15
- 238000001514 detection method Methods 0.000 claims abstract description 124
- 238000006243 chemical reaction Methods 0.000 claims abstract description 64
- 239000000306 component Substances 0.000 claims abstract description 57
- 239000008358 core component Substances 0.000 claims abstract description 52
- 238000010521 absorption reaction Methods 0.000 claims abstract description 15
- 238000002347 injection Methods 0.000 claims abstract description 15
- 239000007924 injection Substances 0.000 claims abstract description 15
- 239000000126 substance Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 4
- 238000005406 washing Methods 0.000 description 20
- 239000006210 lotion Substances 0.000 description 18
- 238000012360 testing method Methods 0.000 description 13
- 239000000758 substrate Substances 0.000 description 10
- 201000003511 ectopic pregnancy Diseases 0.000 description 8
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 7
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 230000000875 corresponding effect Effects 0.000 description 6
- 239000012224 working solution Substances 0.000 description 5
- 230000035935 pregnancy Effects 0.000 description 4
- 239000011435 rock Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000007983 Tris buffer Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
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- 206010024217 lentigo Diseases 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000011002 quantification Methods 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
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- 238000002791 soaking Methods 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
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- 239000004698 Polyethylene Substances 0.000 description 1
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- 239000006180 TBST buffer Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 229960004407 chorionic gonadotrophin Drugs 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 208000002925 dental caries Diseases 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
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- 229930182833 estradiol Natural products 0.000 description 1
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- 229920003023 plastic Polymers 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 229960003387 progesterone Drugs 0.000 description 1
- 239000000186 progesterone Substances 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 1
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- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention provides a kind of high flux immunoassay device.The immunoassay device includes：Core component is detected, the outer wall close to described detection core component one end is provided with detection reaction zone；And casing component, one end of the casing component is provided with injection port, multipair fill orifice is provided with the side wall of the casing component, the inwall of the casing component, which is provided between multipair containment member, containment member described in each pair, is provided with absorption member.During assembling, the detection core component is inserted into the casing component so that described one end of the detection core component is adjacent to described one end of the casing component.After being completed, multiple independent Cavity structural members are formed between outer wall, the inwall of the casing component and the containment member of the detection core component, each described Cavity structural member is connected with fill orifice described in a pair respectively.
Description
Technical field
It is from a kind of sample while detecting the fast high-flux detection means of a variety of thing content concns to be checked the present invention relates to a kind of immunoassay device of biological immunology detection technique field.
Background technology
With the development of immunoassay technology, high flux, accurate quantification and it is portable easy to operate the features such as to detect product basic demand, it is simple to operate that this product detects that product has relative to traditional ELISA, and, multi objective small to sample requirements is while the advantage such as detection；Comparing for the products such as collaurum has detection flux high, can the advantage such as more accurate quantification, this product can be suitable for artificial and machine simultaneously and distinguish.
The content of the invention
The present invention provide it is a kind of it is simple to operate, the immunoassay device that multi objective is quantitatively detected can be carried out.
The immunoassay device includes：Core component (1) is detected, the outer wall close to described detection core component (1) one end is provided with detection reaction zone (5)；With casing component (2), one end of the casing component (2) is provided with injection port (16), multipair fill orifice (6) is provided with the side wall of the casing component (2), the inwall of the casing component (2) is provided with multipair containment member (9), and absorption member (8) is provided between containment member (9) described in each pair.During assembling, the detection core component (1) is inserted into the casing component (2) so that described one end of the detection core component (1) is adjacent to described one end of the casing component (2).After being completed, multiple independent Cavity structural members are formed between outer wall, the inwall of the casing component (2) and the containment member (9) of the detection core component (1), each described Cavity structural member is connected with fill orifice described in a pair (6) respectively.
Preferably, the detection reaction zone (5) includes multiple reaction subregions, circumferentially-spaced being uniformly distributed of the multiple reaction subregion along the detection core component (1), the multiple reaction subregion can be according to being administered simultaneously identical or different chemical composition the need for immune detection, for being caught to thing to be checked or occurring chemical reaction with the thing to be checked and the signal that can be recorded is presented.
Preferably, five independent Cavity structural members are formed between outer wall, the inwall of the casing component (2) and the containment member (9) of the detection core component (1).
Preferably, the other end of the detection core component (1) is provided with handle component (3), and the detection core component (1) is advanced into the casing component (2) or pulled out from the casing component (2) by gripping the handle component (3) during operation.
Preferably, the other end of the casing component (2) is provided with housing handle (10), and the casing component (2) is consolidated by gripping the housing handle (10) during operation.
Preferably, the high flux immunoassay device also includes fill orifice cap (7), and the fill orifice cap (7) is used to close the fill orifice (6).
Preferably, the high flux immunoassay device also includes concentration ratio to chi (18), the concentration ratio is arranged on the outer wall of casing component (2) to chi (18), for being used as reference when detection signal and reading thing concentration value to be checked.
The immunoassay device that the present invention is provided realizes that the integrated high flux of multi objective is detected using immunology principle, the characteristics of with chip；Required detection sample is few, at least only needs to 100 microlitres；It is simple to operate；Sensitivity is high, can accomplish accurate quantification；As a result reading both can also can by visual observation compare progress using instrument and equipment analysis.
Brief description of the drawings
Fig. 1 is the tangent plane schematic diagram for the detection means for showing an embodiment of the invention.
Fig. 2 is the schematic perspective view for showing the detection core component in Fig. 1.
Fig. 3 is the schematic perspective view for showing the casing component in Fig. 1
Embodiment
The specific embodiment of the present invention is described in detail below in conjunction with accompanying drawing.
As shown in figure 1, this detection means includes detection core component 1 and casing component 2.
Detection core component 1 is cylindrical in shape, such as, with the shape similar to syringe piston core, can be made up, be preferably made up of polystyrene of materials such as polystyrene, polyvinyl chloride, polyethylene, is that light color is opaque, preferably milky.
In the outer wall close to detection core component 1 one end provided with detection reaction zone 5, detection reaction zone 5 can include multiple reaction subregions, they are uniformly distributed along detection the circumferentially-spaced of core component 1, each reaction subregion according to detection can need that identical or different chemical composition is administered simultaneously, the chemical composition can be caught or be chemically reacted to thing to be checked and be presented the signal that can be recorded, for example, identical or different antigen or antibody is coated with each reaction subregion, for being reacted with the thing to be checked in measuring samples.
With detection reaction zone 5 close to position instruction line 4 around detection core component 1 is set, for indicating detection reaction zone 5 position in a device.Instruction line 4 can be located on the direction of propulsion of detection core component 1, detect the upstream of reaction zone 5 or the position in downstream.Instruction line 4 shown in Fig. 2 is located at the upstream position of detection reaction zone 5.
The other end of detection core component 1 can be provided with handle component 3, be easy to operator to hold and promoted or pulling motion.
Casing component 2 is cylindrical in shape, and can be made up of normal transparent plastic material.Multipair fill orifice 6 is provided with casing component 2.Each fill orifice 6 is closed by fill orifice cap 7.
The madial wall of casing component 2 sets multipair annular containment member 9, and the length direction of the multipair containment member 9 along casing component 2 is spaced apart.The particular number of containment member 9 depends on the quantity of cavity described hereinafter.Containment member 9 can be rubber ring.All being set respectively between each pair containment member 9 includes absorbent material in an absorption member 8, absorption member 8.The quantity of absorption member 8 is corresponding with the logarithm of containment member 9.
One end of casing component 2 is provided with injection port 16, and injection port 16 is closed by injection port cap 17.
The other end of casing component 2 can be provided with housing handle 10, be promoted or during draw operation to detection core component 1, and operator holds the handle 10 and holds casing component 2 with firm, so as to help will successfully detect that core component 1 be promoted or pulled out.
During assembling, one end that the vicinity for detecting core component 1 is provided with into detection reaction zone 5 is inserted into casing component 2, the other end is exposed at the outside of casing component 2, so detection core component 1 handle component 3 than casing component 2 housing handle 16 more outward so that be user-friendly for detect core component 1 propulsion or draw operation.
After detection core component 1 is completed with casing component 2, the outer wall of detection core component 1 is brought into close contact with containment member 9, is so surrounded by the outer wall of detection core component 1, the inwall of casing component 2 and containment member 9 and is formed multiple independent toroidal cavities.The particular number of toroidal cavity depends on specific immune detection flow.Each toroidal cavity is connected with a pair of fill orifices 6 respectively, is needed to irrigate identical or different solution into each toroidal cavity according to detection by fill orifice 6.
As shown in Fig. 2 foring five cavitys in the device of present embodiment altogether, explanation hereinafter, is referred to as reaction chamber 11, the first washing lotion chamber 12, test chamber 13, the second washing lotion chamber 14 and substrate chamber 15 successively since detection means for convenience one end of injection port 16.It can be needed by respective fill orifice 6 according to detection respectively toward identical or different solution is irrigated in each above-mentioned cavity, fill and covered fill orifice cap 7 afterwards.Accordingly, as shown in Figures 2 and 3, containment member 9 is provided with five pairs in present embodiment.
When being detected with above-mentioned detection device to sample, one hand of operator holds housing handle 16, another hand holds detection core handle 3, injection port 16 is immersed in sample, then detection core component 1 is pulled until instruction line 4 is moved to the position of the containment member 9 of reaction chamber 11, and now measuring samples are inhaled into reaction chamber 11.
After measuring samples absorption is finished, the direction of adjustment detection means causes injection port 16 straight up, measuring samples are contacted with detection reaction zone 5, the identical or different chemical substance being applied on detection reaction zone 5 starts " with reference to " or reacted with thing to be checked, for example, the different antibody that catch being coated on detection reaction zone 5 start " to catch " each self-corresponding object in measuring samples, the identical or different seizure antibody being coated on detection reaction zone 5, which starts " to catch ", can gently rock whole detection means in each self-corresponding object, course of reaction in measuring samples.
After reaction is finished, detection core component 1 is further pulled on so that detection reaction zone 5 is by one of absorption member 8, so that the sample liquids of residual are blotted.Then, pull detection core component 1 detection reaction zone 5 is entered the first washing lotion chamber 12, detection reaction zone 5 is washed in the first washing lotion chamber 12, detection core component 1 is pulled on by one of absorption member 8, so that the washing lotion of residual is blotted.It is then detected that core component 1 enters test chamber 13, whole course of reaction can gently rock whole detection means.In the process, if detecting measuring samples using double sandwich method principles, the target thing to be checked in sample has been captured due to detection reaction zone 5, then each detection antibody of corresponding HRP marks can be combined with thing to be checked respectively in test chamber 13.If detecting thing to be checked using competition law principle, the object in sample is captured due to detection reaction zone 5, then each conjugate to be checked of corresponding HRP marks can be combined with catching the sky " binding site " not occupied on antibody by target thing to be checked in test chamber 13.
After reaction is completed, further pulling on detection core component 1 makes detection reaction zone 5 be washed for second into the progress of the second washing lotion chamber 14, and afterwards again by one of absorption member 8, the detection liquid of residual is blotted.Next, detect that reaction zone 5 enters substrate chamber 15, if there is object in sample, the chemical substance so now applied on detection reaction zone 5 will interact with thing to be checked is presented the signal that can be recorded and quantify, for example, it is coated on object of the antibody capture on detection reaction zone 5 into sample, object is again with detecting antibody binding and being fixed on detection reaction zone 5, the HRP substrate for enzymatic activity that marks forms calm color spot in detection reaction zone 5 on detection antibody, sink the lentiginose depth with target thing to be checked into positive correlation or negative correlation.
In order to more quickly and easily read testing result, on casing component 2 concentration ratio can be set to be used as reference to chi 18, according to substrate color depth, the concentration of object to be measured is read to chi 18 with reference to the concentration ratio.Concentration ratio can be formed in the following manner to chi 18：Using the standard sample of various concentrations, the color state of thing to be checked at various concentrations is recorded, acquisition graded concentration chi figure is drawn for example, by computer, concentration ratio is ultimately formed to chi by debugging, and be printed on the casing component 2 of detection means.
Embodiment
The application of the detection means of the present invention in practice is further described so that ectopic pregnancy is detected as an example below.
According to prior art and the data in our company laboratory, the present embodiment simultaneous selection human chorionic gonadotrophin (β-HCG), progesterone (P), estradiol (E2) and VEGF (VEGF) as ectopic pregnancy joint inspection index.
(1) coating of antibody is caught
Four subregions are set in the detection reaction zone 5 positioned at detection core component 1 one end, coating is directed to β-HCG, P, E2 and VEGF seizure antibody respectively, with 0.05M carbonate buffer solution (specific formulas：0.014M Na2CO3, 0.035M NaHCO3PH 9.6) anti-β-HCG, P, E2 and VEGF monoclonal antibody are diluted to 10ug/ml concentration respectively, it is added dropwise respectively in corresponding subregion, every kind of antibody point 10ul, it is to adsorb 16 hours in the environment of 80% in 4 DEG C, humidity, whole detection reaction zone 5 is immersed into the 0.01M TBS solution containing 2%BSA afterwards, and (specific formula is：0.01M Tris, 0.15M NaCl, pH 7.5) in closing 2 hours, then dry standby.
(2) perfusion of various buffer solutions
After the completion of the coating for catching antibody, detection core component 1 is inserted in casing component 2, the cavity of annular is formed between sealing ring (containment member 9) on detection core component 1 and casing component 2, reaction chamber 11, the first washing lotion chamber 12, test chamber 13, the second washing lotion chamber 14 and substrate chamber 15 are followed successively by from the detection top of core component 1 to detection core handle 3 direction.The corresponding liquid of each intra-bladder instillation is given by respective fill orifice 6.0.05M TBST is irrigated in first washing lotion chamber 12 and the second washing lotion chamber 14, and (specific formula is：0.05M Tris, 0.15M NaCl, 0.05%Tween-20, pH 7.4) it is used as washing lotion.The liquid irrigated in test chamber 13 is the P coupled complex working solutions of anti-β-HCG detections antibody working solution, the anti-β-HCG detection antibody working solution of HRP marks, the E2 coupled complexes working solution of HRP marks and HRP marks that HRP is marked, and above-mentioned working solution detects antibody by the anti-β-HCG for marking HRP, the anti-β-HCG of HRP marks detect that (specific formula is with 0.05M TBS buffer solutions for the P coupled complexes of antibody, the E2 coupled complexes of HRP marks and HRP marks：0.05M Tris, 0.15M NaCl, pH 7.4) it is adjusted to concentration to distinguish all to be 1mg/ml, while the bovine serum albumin(BSA) (BSA) for adding final concentration of 1% is formulated as protective agent and final concentration of 1 ‰ Proclin300 as preservative.What is irrigated in substrate chamber 15 is sedimentation type one pack system TMB solution (good fortune is produced because of Deco skill (Wuhan) Co., Ltd, and article No. is ELS0010P).Fill orifice cap 7 is covered on fill orifice 6 after having irrigated, injection port cap 17 is covered in injection port 16.So far, ectopic pregnancy immunoassay device assembling is completed, and is placed in 4 DEG C and is saved backup.
(3) preparation of the concentration ratio to chi
Detect β-HCG, the normal concentration sample of P, E2 and VEGF under various concentrations, record the color state under each concentration, survey the numerical value that grate goes out the corresponding different things (β-HCG, P, E2 and VEGF) to be checked of different colours depth, graded concentration chi is drawn with computer, concentration ratio is ultimately formed to chi by debugging, is printed on the casing component 2 of the detection means for detecting ectopic pregnancy.
(4) detection of ectopic pregnancy and normal pregnancy blood sample
Assist to collect normal pregnancy by gynemetrics of central hospital of Xiangfan City and the later stage confirms Ectopic Pregnancy Patients serum, pregnancy time is 40 ± 5 days, respectively collects 40 patients serums.Thing (β-HCG, P, E2 and VEGF) to be checked had all carried out measure by radioimmunology in these serum, and concrete condition is shown in Table 1.
The injection port cap of detection means is removed, injection port is immersed in measuring samples, blood sample 100ul to be checked is drawn from injection port 16, pulls detection core component 1 to its instruction line 4 to be moved at the sealing ring of reaction chamber 11, now measuring samples are inhaled into reaction chamber 11.
After measuring samples absorption is finished, the direction of adjustment detection means causes injection port 16 straight up, the different antibody that catch on detection reaction zone 5 start " to catch " each self-corresponding object in measuring samples liquid, and the reaction time is 5 minutes, and whole course of reaction can gently rock whole detection means.
After reaction is finished, detection core component 1 to detection reaction zone 5 is pulled to enter in the first washing lotion chamber 12, in the process, detection reaction zone 5 streaks one of absorption member 8, the sample liquid of residual is blotted, into after the first washing lotion chamber 12, is stopped in the first washing lotion chamber 12 2 minutes and is carried out washing by soaking.Next, detection reaction zone 5 passes through absorption member 8, the washing lotion of residual is blotted, subsequently into test chamber 13, and reaction is 5 minutes in test chamber 13, and whole course of reaction can gently rock whole detection means.In the process, if detecting thing to be checked using double sandwich method principles, detection zone has captured the thing to be checked in sample, then the detection antibody of each HRP marks in test chamber 13 is just combined with thing to be checked respectively.If detecting thing to be checked using competition law principle, detection zone has captured the thing to be checked in sample, then the conjugate to be checked of each HRP marks in test chamber 13 can only be combined with catching the sky " binding site " not occupied on antibody by target thing to be checked.
After reaction in test chamber 13 terminates, pulling on detection core component 1 makes detection reaction zone 5 enter the second washing lotion chamber 14, in the process, and detection reaction zone 5 passes through absorption member 8, residue detection liquid is blotted, and the washing by soaking of 2 minutes is passed through in the second washing lotion chamber 14.Absorption member 8 is moved through again afterwards, the washing lotion of residual is blotted.Subsequently into the 5th chamber 15, reacted 5 minutes in substrate chamber 15.If having object in sample and object being adapted to be detected with double antibody sandwich method, target antibody so on detection reaction zone catches the object in sample, object is fixed on detection zone with detection antibody binding again, mark has enzyme on detection antibody, HRP substrate for enzymatic activity forms calm color spot on detection reaction zone 5, sinks concentration of the lentiginose depth with object to be checked into positive correlation or negative correlation；If having object in sample and object being adapted to be detected with competition law, the object in antibody capture sample so on detection reaction zone, the site that the object conjugate of HRP marks is not combined with catching on antibody by free object is combined, HRP substrate for enzymatic activity forms calm color spot in detection reaction zone 5, and the lentiginose depth of sinking is negatively correlated with the concentration of object to be checked.The concentration that color depth reads object to be measured with concentration ratio to chi according to substrate.
The ectopic pregnancy detection means testing result of table 1
(remarks：Critical Standard is formulated according to prior art and the detection data in this laboratory, and the sample that this laboratory is chosen is ectopic pregnancy and the blood of normal pregnancy 40 days or so (40d ± 5d).)
Technical scheme is described in detail above in association with embodiment and embodiment, but the present invention is not limited to this.On the premise of the object of the invention is realized, those skilled in the art can make various changes and deformation to the present invention.
Claims (7)
1. a kind of high flux immunoassay device, it includes：
Core component (1) is detected, the outer wall close to described detection core component (1) one end is provided with
Detect reaction zone (5)；With
Casing component (2), injection port (16) is provided with one end of the casing component (2),
Multipair fill orifice (6), the shell structure are provided with the side wall of the casing component (2)
The inwall of part (2) is provided with multipair containment member (9), containment member (9) described in each pair it
Between be provided with absorption member (8),
During assembling, the detection core component (1) is inserted into the casing component (2), is made
Described one end of the detection core component (1) is obtained adjacent to described the one of the casing component (2)
End,
After being completed, in outer wall, the casing component (2) of the detection core component (1)
Inwall and the containment member (9) between form multiple independent Cavity structural members, each
The Cavity structural member is connected with fill orifice described in a pair (6) respectively.
2. high flux immunoassay device according to claim 1, it is characterised in that
The detection reaction zone (5) includes multiple reaction subregions, and the multiple reaction subregion is along described
Circumferentially-spaced being uniformly distributed of core component (1) is detected, the multiple reaction subregion can be according to immune
Identical or different chemical composition is administered simultaneously the need for detection, for being caught to thing to be checked
Or occur to chemically react and present the signal that can be recorded with the thing to be checked.
3. high flux immunoassay device according to claim 1, it is characterised in that
In outer wall, the inwall of the casing component (2) and described of the detection core component (1)
Five independent Cavity structural members are formed between containment member (9).
4. high flux immunoassay device according to claim 1, it is characterised in that
The other end of the detection core component (1) is provided with handle component (3), by holding during operation
Hold the handle component (3) and the detection core component (1) is advanced into the casing component
(2) pulled out in or from the casing component (2).
5. high flux immunoassay device according to claim 1, it is characterised in that
The other end of the casing component (2) is provided with housing handle (10), by gripping during operation
The housing handle (10) consolidates the casing component (2).
6. high flux immunoassay device according to claim 1, it is characterised in that
Also include fill orifice cap (7), the fill orifice cap (7) is used to close the fill orifice (6).
7. high flux immunoassay device according to claim 1, it is characterised in that
Also include concentration ratio to chi (18), the concentration ratio is arranged on casing component (2) to chi (18)
Outer wall, for be used as detection signal and read thing concentration value to be checked when reference.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610222107.XA CN107290515A (en) | 2016-04-11 | 2016-04-11 | High flux immunoassay device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610222107.XA CN107290515A (en) | 2016-04-11 | 2016-04-11 | High flux immunoassay device |
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| Publication Number | Publication Date |
|---|---|
| CN107290515A true CN107290515A (en) | 2017-10-24 |
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| CN201610222107.XA Pending CN107290515A (en) | 2016-04-11 | 2016-04-11 | High flux immunoassay device |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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