CN207891325U - A kind of device of extraction purification nucleic acid - Google Patents

Abstract

The utility model provides a kind of device of extraction purification nucleic acid, can realize automatic sampling without other consumptive materials under the cooperation of external equipment, without sample-adding manually；It is compared with the nucleic acid extraction of traditional open, avoid the cross contamination in nucleic acid extraction and amplification procedure, and the utility model can purify be useful for nucleic acid extraction and subsequently various reagents needed for detection of nucleic acids complete quantitative encapsulation in the shell before extraction purification, so reducing the misoperation probability of experiment operator operation simultaneously, nucleic acid can also be automatically imported under amplification condition and expand by the utility model, without operating again so that operating comfort higher.

Description

A kind of device of extraction purification nucleic acid

Technical field

The utility model belongs to medical instruments field, is related to a kind of device of extraction purification nucleic acid.

Background technology

Nucleic acid be by many nucleotide polymerizations at large biological molecule compound, be the carrier of organism hereditary information, For one of the most basic substance of life, therefore the research of nucleic acid sequence and structure is either carried out, or to gene function, expression It is explored, also or carries out genetic engineering, protein engineering, be required for that nucleic acid is detached and purified first.

Currently, having the device for nucleic acid extraction purifying in the market, can be used for extracting nucleic acid and purifying, still Such device is during for extracting nucleic acid, after in Sample introduction to mixing chamber, cracking room or reative cell, sample It just will increase with the volume summation of reactant of the original place in mixing chamber, cracking room or reative cell, so allow for mixing chamber, split The state for occurring overvoltage in solution room or reative cell, is thus easy to cause situations such as sample can not be imported normally, the stabilization of reaction Property is relatively low.

Utility model content

The purpose of this utility model is to provide a kind of device and method of extraction purification nucleic acid, it is intended to solve example reaction The low problem of stability.

In order to solve the above technical problems, the utility model provide a kind of device of extraction purification nucleic acid, including shell with And sealing cover, liquid relief chamber, amplification chamber and several reagent chamber are provided in the shell, each reagent chamber is separate One end of the sealing cover offers reaction channel, and the other end offers venting channels, and by the reaction channel and The venting channels are connected to the both ends of the liquid relief chamber respectively, and the both sides of the amplification chamber offer detection and become a mandarin respectively Channel and detection outlet passageway, and by the detection inflow channel and the detection outlet passageway respectively with the liquid relief chamber Connection, further includes having：

Liquid relief body, the liquid relief body are rotationally connected in the liquid relief chamber, and the liquid relief body includes the first of cylindrical shape The one end in liquid relief portion, first liquid relief portion is opening-like, and the other end is provided with liquid outlet, the liquid outlet and the reaction channel Or the detection inflow channel activity cooperation, it is provided with air inlet on the liquid relief body outside first liquid relief portion, institute Air inlet is stated with the detection outlet channel-active to coordinate；

Pipettor, the pipettor are matched with first liquid relief portion shape, and shifting is movably set in the pipettor Liquid piston, the liquid relief piston is for displacing or being drawn into sample in the pipettor；

Liquid relief lid, the liquid relief, which covers, offers liquid relief hole, the liquid relief lid fit in the sealing cover and with it is described close Capping rotation connection, the pipettor may pass through the liquid relief hole and enter in first liquid relief portion, and the liquid relief covers out Equipped with the first air channel and the second air channel, when the liquid relief lid is connect with the liquid relief body, first air channel can turn Dynamic to be connected to the venting channels, first liquid relief portion passes through first air channel and venting channels selection and one Reagent chamber's connection, one end of second air channel are connected to first liquid relief portion, and the other end is pierced by described first and moves The side wall in liquid portion, first liquid relief portion pass through between second air channel and the liquid relief body and first liquid relief portion Space is connected to；

Rotor, the rotor are rotationally connected with the liquid relief lid, and rotation selection is offered on the rotor Hole, rotatably selection is connected to the rotation selecting hole with first air channel or with second air channel, is passed through simultaneously Pipettor described in the rotation selecting hole may be selected and a reagent chamber or the amplification chamber；

Seal, the seal are sealedly attached to the pipettor close to the open end of the sealing cover, pass through described Seal is movably set with piston rod, and the piston rod connect with the liquid relief piston and is used to drive the liquid relief piston to live It is dynamic.

The utility model is further arranged to, and the outer diameter of the pipettor is less than the aperture in the liquid relief hole, described The size of the outer diameter of rotor is equal in magnitude with the aperture in the liquid relief hole.

The utility model is further arranged to, and the second liquid relief portion, second liquid relief portion are additionally provided in the liquid relief body Diameter be less than the diameter in first liquid relief portion, second liquid relief is movably set with detection piston in portion, passes through the shifting Liquid lid is simultaneously flexibly connected with the liquid relief lid and is useful for driving the movable detection bar of the detection piston, is located at second liquid relief Detection hole, the detection hole and the detection inflow channel or reaction channel activity are offered on the liquid relief body in portion Cooperation, the liquid relief, which covers, is further opened with third air channel and the 4th air channel, the third air channel rotatably selection and one The venting channels connection, may be selected by the second liquid relief portion described in the third air channel and the venting channels and described in one Chamber is expanded, the both ends of the 4th air channel are respectively with second liquid relief portion and positioned at second liquid relief portion and institute State the space connection of the liquid relief body between the first liquid relief portion.

The utility model is further arranged to, and sealing rubber pad is provided between the liquid relief body and the liquid relief chamber, It offers on the sealing rubber pad and is matched with the reaction channel, the detection inflow channel and the detection outlet passageway Sealing channel.

The utility model is further arranged to, and the reagent chamber includes heating chamber, for being heated to sample.

The utility model is further arranged to, and heating tank is offered on the shell, and the heating chamber is located at described add In heat channel.

The utility model is further arranged to, and the amplification chamber is provided with several.

A kind of method of extraction purification nucleic acid, includes the following steps：

S1, liquid relief piston activity in pipettor simultaneously suck the sample into pipettor；

S2, pipettor is entered across liquid relief lid in the first liquid relief portion by liquid relief hole, until rotor is engaged to liquid relief Kong Zhong, and rotation selecting hole is made to be directed at the first air channel, the first air channel is accordingly connected to one of venting channels；

S3 drives piston rod and drives liquid relief piston activity by piston rod, sample is pressed into correspondence by liquid outlet Reaction channel in, while the air in reagent chamber pass sequentially through venting channels, the first air channel and rotation selecting hole enter Into the space in pipettor between liquid relief piston and seal；

S4, after waiting for that sample reacts completion in a reagent chamber, piston rod activity and will be through in a manner of opposite with S3 The sample for having crossed first step reaction is drawn into pipettor；

S5, the rotation of liquid relief body, and liquid outlet is made to be directed at the reaction channel of second reagent chamber, while the first air channel connects Lead to the venting channels of second reagent chamber, repeat S3-S4, completes second step processing；

The step of S6, repetition S5, machines until to sample, will be in the sample that machined driving to amplification chamber；

S7 is detected the sample in amplification chamber, completes once to the detection of sample.

The utility model is further arranged to, and the S6 includes：

S61, the rotation of liquid relief body, and detection hole is connected to the reaction channel of the last one reagent chamber, while third is logical Air drain is connected to the venting channels of the reagent chamber；

The sample that reaction is completed is drawn into the second liquid relief portion, is located at simultaneously by S62, detection bar driving detection piston activity Air in second liquid relief portion between detection piston and sealing cover passes sequentially through third air channel and venting channels enter reagent In room；

S63, the rotation of liquid relief body, and detection hole is connected to one of detection inflow channel；

Sample to be detected is driven into amplification chamber, expands simultaneously by S64, detection bar driving detection piston activity Air in chamber is flowed by detecting outlet passageway in liquid relief body, and enters the second liquid relief portion by the 4th air channel In.

The utility model is further arranged to, and when sample enters in the heating chamber, the heating chamber is to sample Product are heated.

Compared with prior art, the utility model provides a kind of device and method of extraction purification nucleic acid, by sample When being sent in amplification chamber or reagent chamber, the air in amplification chamber passes sequentially through detection outlet passageway, liquid relief Body, the second air channel and rotation selecting hole enter in pipettor, or the air in reagent chamber passes sequentially through ventilation Channel, the first air passage and rotation selecting hole enter in pipettor, even if the sample so in pipettor flows into reagent The air that room either expands in chamber in reagent chamber or amplification chamber can be flow into pipettor simultaneously, in this way The atmospheric pressure for allowing for reagent chamber and expanding in chamber maintains a relatively stable state, without excessive pressure The case where power, thus sample also can normally into and reacted, the stability of reaction is high；Simultaneously because sample and air it Between be relative flow, so after sample enters in amplification chamber or reagent chamber, will not occur in pipettor excessive Negative pressure, also can preferably prevent external air from entering in shell in this way, to prevent sample to be contaminated, detection Quality be improved from side.

Description of the drawings

Fig. 1 is the structural schematic diagram of assembled state in a kind of device of extraction purification nucleic acid of the utility model；

Fig. 2 is the exploded perspective view one in a kind of device of extraction purification nucleic acid of the utility model；

Fig. 3 is the enlarged drawing of part A in Fig. 2；

Fig. 4 is the exploded perspective view two in a kind of device of extraction purification nucleic acid of the utility model；

Fig. 5 is pipettor, liquid relief lid, rotor and seal in a kind of device of extraction purification nucleic acid of the utility model Assembling structure schematic diagram；

Fig. 6 be each reaction channel of enclosure in a kind of device of extraction purification nucleic acid of the utility model, detection become a mandarin The structural schematic diagram in channel and detection outlet passageway；

Fig. 7 is the internal structure chart of shell in a kind of device of extraction purification nucleic acid of the utility model；

Fig. 8 is the structural schematic diagram of pipettor and rotor in a kind of device of extraction purification nucleic acid of the utility model；

Fig. 9 is the structural schematic diagram one of liquid relief body in a kind of device of extraction purification nucleic acid of the utility model；

Figure 10 is the structural schematic diagram two of liquid relief body in a kind of device of extraction purification nucleic acid of the utility model；

Figure 11 is the structural schematic diagram of liquid relief lid in a kind of device of extraction purification nucleic acid of the utility model.

Wherein, 1- shells；2- liquid relief chambers；3- expands chamber；4- sealing covers；5- reagent chamber；6- reaction channels；7- ventilates Channel；8- detects inflow channel；9- detects outlet passageway；10- liquid relief bodies；The first liquid reliefs of 11- portion；12- liquid outlets；13- air inlets Mouthful；14- pipettors；15- liquid relief lids；16- liquid reliefs hole；The first air channels of 17-；The second air channels of 18-；19- rotors；20- turns Dynamic selecting hole；21- seals；The second liquid reliefs of 22- portion；23- detection holes；24- third air channels；The 4th air channels of 25-；26- is close Seal rubber pad；27- seals channel；28- heating chambers；29- heating tanks.

Specific implementation mode

Below in conjunction with the drawings and specific embodiments to the utility model proposes a kind of extraction purification nucleic acid device and side Method is described in further detail.According to following explanation and claims, will be become apparent from feature the advantages of the utility model.It needs Illustrate, attached drawing is all made of very simplified form and uses non-accurate ratio, only to convenient, lucidly auxiliary is said The purpose of bright the utility model embodiment.Same or analogous reference numeral represents same or analogous component in attached drawing.

Embodiment 1

A kind of device of extraction purification nucleic acid, as shown in Figure 1 to 11, including shell 1 and sealing cover 4, the shell 1 In be provided with liquid relief chamber 2, amplification chamber 3 and several reagent chamber 5, the wherein size of reagent chamber 5 can be according to practical feelings Condition selects identical or different size, wherein amplification chamber 3 is used for nucleic acid amplification, and with external equipment cooperation to amplification Nucleic acid be detected, wherein amplification chamber 3 structure be loop configuration, upper and lower ends by film cover so as to Nucleic acid is contained；Wherein the amplification chamber 3 is provided with several, four is taken in the present embodiment, four amplification chambers 3 In in same plane, convenient for being used with extraneous cooperating equipment, the amplification chamber 3 between chamber 3 and shell 1 is each expanded It is provided with deformation hole in structure, shell 1 can be reduced by deformation hole and expands the transmission of heat between chamber 3, in this way in nucleic acid Reaction and detection when, smaller influence can be generated between each other between two processes；Each reagent chamber 5 is separate One end of the sealing cover 4 offers reaction channel 6, and the other end offers venting channels 7, and passes through the reaction channel 6 and the venting channels 7 be connected to respectively with the both ends of the liquid relief chamber 2, it is described amplification chamber 3 both sides offer inspection respectively Survey inflow channel 8 and detection outlet passageway 9, and by the detection inflow channel 8 and the detection outlet passageway 9 respectively with institute The connection of liquid relief chamber 2 is stated, detection inflow channel 8 and detection outlet passageway 9 are communicated in the both ends of the amplification chamber 3, China and foreign countries Shell 1, liquid relief chamber 2, amplification chamber 3, reagent chamber 5, reaction channel 6, venting channels 7, detection inflow channel 8 and detection outlet are logical Road 9 is that integrated injection molding is made, while being molded with several grooves in 1 bottom end of shell, and is covered with film, on film and shell 1 Groove form reaction channel 6, state the detection inflow channel 8 and detection outlet passageway 9, further include having：

Liquid relief body 10, the liquid relief body 10 are rotationally connected in the liquid relief chamber 2, and are in the liquid relief chamber 2 It is full of cooperation, this makes it possible to prevent air from entering in the space between liquid relief body 10 and liquid relief chamber 2, between liquid-to-air Convection effects it is more preferable；The liquid relief body 10 includes the first liquid relief portion 11 of cylindrical shape, and the one end in first liquid relief portion 11 is in Openning shape, the other end are provided with liquid outlet 12, the liquid outlet 12 and 8 activity of the reaction channel 6 or the detection inflow channel Cooperation, is provided with air inlet 13 on the liquid relief body 10 outside first liquid relief portion 11, the air inlet 13 with it is described Detect 9 activity cooperation of outlet passageway；

Pipettor 14, the pipettor 14 are matched with first liquid relief portion, 11 shape, movable in the pipettor 14 It is provided with liquid relief piston, the liquid relief piston is used to that sample to be displaced or is drawn into the pipettor 14, wherein pipettor 14 i.e. for from extraneous pipette samples, while when detection, also function to and shift sample between different chamber Effect；

Liquid relief lid 15, offers liquid relief hole 16 on the liquid relief lid 15, the liquid relief lid 15 fit in the sealing cover 4 and It is rotatablely connected with the sealing cover 4, the connection type of the two is that the diameter of liquid relief lid 15 is less than the opening on sealing cover 4, simultaneously Sealing cover 4 is closely attached on side of the liquid relief lid far from shell 1, and such liquid relief lid is adjacent to sealing cover 4, while the two is not In the same plane, so being still able to keep a preferable sealing state by liquid relief lid 15 and 4 shell of sealing cover；It is described Pipettor 14 may pass through the liquid relief hole 16 and enter in first liquid relief portion 11, and it is logical that first is offered on the liquid relief lid 15 Air drain 17 and the second air channel 18, when the liquid relief lid 15 is connect with the liquid relief body 10, first air channel 17 can turn Dynamic to be connected to the venting channels 7, first liquid relief portion 11 is selected by first air channel 17 and the venting channels 7 It selects and is connected to a reagent chamber 5, one end of second air channel 18 is connected to first liquid relief portion 11, and the other end is pierced by The side wall in first liquid relief portion 11, first liquid relief portion 11 pass through second air channel 18 and the liquid relief body 10 and institute State the space connection between the first liquid relief portion 11；

Rotor 19, the rotor 19 are rotationally connected with the liquid relief lid 15, are offered on the rotor 19 Rotate selecting hole 20, the rotation selecting hole 20 rotatably selection and first air channel 17 or with second air channel 18 connections, while may be selected and a reagent chamber 5 or the amplification chamber by pipettor 14 described in the rotation selecting hole 20 Room 3 is connected to；

Seal 21, the seal 21 are sealedly attached to the pipettor 14 close to the open end of the sealing cover 4, wear It crosses the seal 21 and is movably set with piston rod, the piston rod connect with the liquid relief piston and is used to drive the liquid relief Piston activity.

The outer diameter of the pipettor 14 is less than the aperture in the liquid relief hole 16, the size of the outer diameter of the rotor 19 It is equal in magnitude with the aperture in the liquid relief hole 16.The second liquid relief portion 22 is additionally provided in the liquid relief body 10, described second moves The diameter in liquid portion 22 is less than the diameter in first liquid relief portion 11, wherein the volume in the first liquid relief portion 11 is 500-1500ml sheets Embodiment takes 1000ml, and the volume in the second liquid relief portion 22 is 20-500ml, and the present embodiment is preferably 100ml, second liquid relief It is movably set with detection piston in portion 22, is flexibly connected across the liquid relief lid 15 and with the liquid relief lid 15 and is useful for driving institute The detection movable detection bar of piston is stated, detection hole 23 is offered on the liquid relief body 10 in second liquid relief portion 22, The detection hole 23 coordinates with 8 or described reaction channel of the detection inflow channel, 6 activity, is further opened on the liquid relief lid 15 Third air channel 24 and the 4th air channel 25, rotatably selection is connected to the third air channel 24 with the venting channels 7, is led to The second liquid relief portion 22 described in the third air channel 24 and the venting channels 7 is crossed to may be selected to be connected to an amplification chamber 3, The both ends of 4th air channel 25 are respectively with second liquid relief portion 22 and positioned at second liquid relief portion 22 and described first The space of the liquid relief body 10 between liquid relief portion 11 is connected to.

Sealing rubber pad 26 is provided between the liquid relief body 10 and the liquid relief chamber 2, on the sealing rubber pad 26 Offer with 9 matched sealing channel 27 of the reaction channel 6, the detection inflow channel 8 and the detection outlet passageway, The reagent chamber 5 includes heating chamber 28, and for being heated to sample, heating tank 29 is offered on the shell 1, described Heating chamber 28 is located in the heating tank 29, and it is hemispherical dome structure that wherein heating chamber 28, which is located at the part in heating tank 29, So when being heated, when being heated to the liquid of same volume, hemispherical dome structure enables to the heating surface area of liquid Maximum, heating effect are more preferable.

A kind of method of extraction purification nucleic acid, includes the following steps：

S1, liquid relief piston activity in pipettor 14 simultaneously suck the sample into pipettor 14；

S2, pipettor 14 is entered across liquid relief lid 15 in the first liquid relief portion 11 by liquid relief hole 16, until rotor 19 It is engaged in liquid relief hole 16, and rotation selecting hole 20 is made to be directed at the first air channel 17, the first air channel 17 is accordingly connected to wherein One venting channels 7；

S3, driving piston rod simultaneously drive the activity of liquid relief piston by piston rod, and it is right that sample is pressed by liquid outlet 12 In the reaction channel 6 answered, while the air in reagent chamber 5 passes sequentially through venting channels 7, the first air channel 17 and rotation selection Hole 20 enters in the space in pipettor 14 between liquid relief piston and seal 21；

S4, after waiting for that sample reacts completion in a reagent chamber 5, piston rod activity and will be through in a manner of opposite with S3 The sample for having crossed first step reaction is drawn into pipettor 14；

S5, liquid relief body 10 rotate, and liquid outlet 12 is made to be directed at the reaction channel 6 of second reagent chamber 5, while first is logical Air drain 17 is connected to the venting channels 7 of second reagent chamber 5, repeats S3-S4, completes second step processing；

The step of S6, repetition S5, machines until to sample, by the sample machined driving to amplification chamber 3 In；

S7 is detected the sample in amplification chamber 3, completes once to the detection of sample.

Wherein, the S6 includes：

S61, liquid relief body 10 rotate, and detection hole 23 is connected to the reaction channel 6 of the last one reagent chamber 5, simultaneously Third air channel 24 is connected to the venting channels 7 of the reagent chamber 5；

S62, the sample that reaction is completed is drawn into the second liquid relief portion 22 by detection bar driving detection piston activity, while position Detected in the second liquid relief portion 22 air between piston and sealing cover 4 pass sequentially through third air channel 24 and venting channels 7 into Enter into reagent chamber 5；

S63, liquid relief body 10 rotate, and detection hole 23 is connected to one of detection inflow channel 8；

Sample to be detected is driven into amplification chamber 3, expands simultaneously by S64, detection bar driving detection piston activity Increase the air in chamber 3 to be flowed into liquid relief body 10 by detecting outlet passageway 9, and second is entered by the 4th air channel 25 In liquid relief portion 22.

Preferably, when sample enters in the heating chamber 28, the heating chamber 28 heats sample.

In conclusion a kind of device and method of extraction purification nucleic acid provided by the utility model, carry to sample Before taking purifying, sealing cover 4 is located on shell 1, is located at the both ends difference of the amplification chamber 3 and heating chamber 28 in shell 1 For the structure of 1 ontology of shell and sealing cover 4；Liquid relief body 10 is located in cylindric liquid relief chamber 2 simultaneously, and can be in liquid relief 360 degree of rotations are carried out in chamber 2, there is circular opening on sealing cover 4, liquid relief lid 15 is located in the opening, and liquid relief lid 15 It can 360 degree of rotations of the progress of opposing seal lid 4；Liquid relief hole 16 on liquid relief lid 15 is located at the surface of pipettor 14, and liquid relief hole 16 aperture is equal with the cylindrical diameter of pipettor 14, the two coaxial arrangement, while rotating liquid relief lid 15 and making first Air channel 17 is connected to the venting channels 7 of one of reagent chamber 5, which is the reagent that sample carries out first step reaction Room 5, when the first air channel 17 is connected to the venting channels 7 of the reagent chamber 5, the reaction channel 6 of the reagent chamber 5 also simultaneously with shifting The liquid outlet 12 of liquid 10 communicates.

Wherein, reagent chamber 5 includes cracking room, reagent one encapsulates room, reagent two encapsulates room, reagent three encapsulates room, reagent Four encapsulation rooms, Nucleic Acid Elution room and nucleic acid amplification room etc., be respectively used to crack sample, add several reaction reagents, Nucleic acid is eluted and is expanded etc., and is detected being passed into amplification chamber 3 after amplification is completed.

When shifting sample, in pipettor 14 piston rod activity and drive liquid relief piston is formed in pipettor 14 bear Then pressure the transfer of pipettor 14 and enters the so that sample to be detected to be drawn into pipettor 14 by liquid relief hole 16 In one liquid relief portion 11, at this time since pipettor 14 enters in the first liquid relief portion 11 so that the air pressure in the first liquid relief portion 11 increases, But since the outer diameter of pipettor 14 is less than the diameter in liquid relief hole 16, so the air of increased air pressure can pass through Outside is flowed out between pipettor 14 and liquid relief hole 16, without causing the air pressure between shell 1 and sealing cover 4 to increase, is continued Mobile pipettor 14 is engaged to until the rotor 19 on pipettor 14 in liquid relief hole 16, completes the assembly of pipettor 14, at this time Since rotor 19 is mutually matched with liquid relief hole 16, it so can preferably prevent external air from entering shell 1 In, detection quality is guaranteed.

After the assembly of pipettor 14 is completed, rotates rotor 19 and rotation selecting hole 20 and the first air channel 17 are connected It is logical, liquid relief piston is then pressed downwards by piston rod, sample is forced into outside pipettor 14, sample passes through on liquid relief body 10 Liquid outlet 12 flow into reaction channel 6, continue to push sample, sample flow by reaction channel 6 in reagent chamber 5, and So that the air pressure in reagent chamber 5 increases, the effect of being under pressure of the air in reagent chamber 5 at this time is flowed away towards venting channels 7, and according to The secondary air for flowing into pipettor 14 by venting channels 7, the first air channel 17 and rotation selecting hole 20, and flowing into, which is located at, to be moved Between liquid piston and seal 21, since liquid relief piston is downwards mobile, between such liquid relief piston and seal 21 Space air pressure is reduced by, and lucky ability allows the air in reagent chamber 5 to flow at this time, so since the air pressure in reagent chamber 5 can As soon as keeping a normal number, such sample can normally be flow into reagent chamber 5, and the stability of detection is high, moreover, It is excessive this makes it possible to preferably prevent from being formed in pipettor 14 due to flowing into the air in reagent chamber 5 in pipettor 14 Negative pressure prevents external air to flow into shell 1 and pollute sample, the safety of reaction and steady is improved from side It is qualitative.

After reaction is completed in a reagent chamber 5, piston rod drives liquid relief piston upwards, while sample is flow back into In pipettor 14, air is flow back into first reagent chamber 5；Liquid relief body 10 rotates, and makes the first air channel 17 alignment second A reagent chamber 5, while liquid outlet 12 is also targeted by the reaction channel 6 of second reagent chamber 5, piston rod activity and drive sample into Enter into second reagent chamber 5 and carry out the reaction of second step, so reacts the sample after completing until example reaction completion in the past Grade is in the last one reagent chamber 5；Liquid relief body 10 rotates, and the detection hole 23 of 22 bottom end of the second liquid relief portion is aligned finally The reaction channel 6 of one reagent chamber 5, detection bar drive up detection piston and the sample that reaction is completed are drawn into the second liquid relief portion In 22, while the air in the second liquid relief portion 22 passes sequentially through third air channel 24, rotation selecting hole 20 and venting channels 7 and enters Into a last reagent chamber 5；After sample enters in the second liquid relief portion 22, liquid relief body 10 rotates and makes detection hole again Sample is simultaneously passed sequentially through detection hole 23 and detection becomes a mandarin by 23 alignment detection inflow channels 8, detection bar driving detection piston activity In the driving of channel 8 to amplification chamber 3, while expanding the air in chamber 3 and being flow into liquid relief body 10 by detecting outlet passageway 9 In space outside pipettor 14 and the second liquid relief portion 22, simultaneously because being by between this space and the second liquid relief portion 22 What four air channels 25 communicated, expanding the air in chamber 3 in this way can normally be flowed into amplification chamber 3, so not only Sample is normally flow into amplification chamber 3 to be detected, while also preventing occurring at the second liquid relief portion 22 Big negative pressure, reaction stability is high, and sample can be detected normally after entering in reagent chamber 5.

Wherein since amplification chamber 3 has several, several groups detection so can be also carried out, inspection is submitted to reach The purpose of mass metering；Simultaneously because heating chamber 28 can heat sample, the speed of reaction can be so improved, is examined It surveys efficient, while can also improve the reaction effect between sample and detection reagent；Heating tank 29 is so that heating chamber 28 is right The heating effect of sample is more preferable.

Wherein, there is sealing rubber pad 26 between liquid relief body 10 and liquid relief chamber 2, due to close on sealing rubber pad 26 Envelope channel 27 is matched with reaction channel 6, detection inflow channel 8, detection outlet passageway 9, so can normally be made Sample and air are obtained by sealing rubber pad 26, while sealing rubber pad 26 is so that between liquid relief body 10 and liquid relief chamber 2 Sealing effect more preferably, this makes it possible to preferably prevent sample from flowing between liquid relief body 10 and liquid relief chamber 2, improves reaction Stability.

Embodiment 2

A kind of device of extraction purification nucleic acid, difference from example 1 is that, the sample transfer completed will reacted Under entering to the method in amplification chamber 3：After example reaction is completed in the last one reagent chamber 5, piston rod drives liquid relief piston Activity simultaneously suck the sample into pipettor 14, pipettor 14 rotate, and make liquid outlet 12 be right against one of them detect into At circulation road 8, rotation rotor 19 is simultaneously so that rotation selecting hole 20 is directed at the second air channel 18, piston rod activity downwards and by sample Product are driven into amplification chamber 3, while being expanded the air in chamber 3 and being entered liquid relief body 10 by detecting outlet passageway 9 In space outside middle pipettor 14 and the second liquid relief portion 22, simultaneously because being by the second ventilation between the space and pipettor 14 What slot 18 and rotation selecting hole 20 communicated, the air so expanded in chamber 3 can be flow into pipettor 14, to both make Sample can stablize it is normal flow into amplification chamber 3, while can also prevent from forming excessive negative pressure in pipettor 14 The occurrence of to cause extraneous air to flow into shell 1, improves the stability of reaction from side；Wherein due to second The diameter in liquid relief portion 22 is smaller, and pipettor 14 is relatively large in diameter, so when the sample size that reaction is completed is more, just needs It to be shifted by pipettor 14, just need to carry out by pipettor 14 when the sample that reaction is completed is less on the contrary, The transfer part of different size can be selected according to actual conditions, it is highly practical.

It should be noted that each embodiment is described by the way of progressive in this specification, each embodiment emphasis is said Bright is all difference from other examples, and just to refer each other for identical similar portion between each embodiment.

Foregoing description is only the description to the utility model preferred embodiment, not to any limit of the scope of the utility model Calmly, any change, the modification that the those of ordinary skill in the utility model field does according to the disclosure above content, belong to right and want Seek the protection domain of book.

Claims (7)

1. a kind of device of extraction purification nucleic acid, which is characterized in that including shell and sealing cover, shifting is provided in the shell Sap cavity room, amplification chamber and several reagent chamber, the one end of each reagent chamber far from the sealing cover offer instead Answer channel, the other end offers venting channels, and by the reaction channel and the venting channels respectively with the liquid relief The both ends of chamber are connected to, and the both sides of the amplification chamber offer detection inflow channel and detection outlet passageway respectively, and pass through The detection inflow channel and the detection outlet passageway further include having respectively with the liquid relief chamber：

Liquid relief body, the liquid relief body are rotationally connected in the liquid relief chamber, and the liquid relief body includes the first liquid relief of cylindrical shape The one end in portion, first liquid relief portion is opening-like, and the other end is provided with liquid outlet, the liquid outlet and the reaction channel or institute State detection inflow channel activity cooperation, be provided with air inlet on the liquid relief body outside first liquid relief portion, it is described into Gas port coordinates with the detection outlet channel-active；

Pipettor, the pipettor are matched with first liquid relief portion shape, and liquid relief work is movably set in the pipettor Plug, the liquid relief piston is for displacing or being drawn into sample in the pipettor；

Liquid relief lid, the liquid relief, which covers, offers liquid relief hole, the liquid relief lid fit in the sealing cover and with the sealing cover Rotation connection, the pipettor may pass through the liquid relief hole and enter in first liquid relief portion, and the liquid relief, which covers, to be offered First air channel and the second air channel, when the liquid relief lid is connect with the liquid relief body, first air channel rotatably with The one venting channels connection, first liquid relief portion pass through described in first air channel and venting channels selection and one Reagent chamber is connected to, and one end of second air channel is connected to first liquid relief portion, and the other end is pierced by first liquid relief portion Side wall, first liquid relief portion passes through the space between second air channel and the liquid relief body and first liquid relief portion Connection；

Rotor, the rotor are rotationally connected with the liquid relief lid, and rotation selecting hole, institute are offered on the rotor Stating rotation selecting hole, rotatably selection is connected to first air channel or with second air channel, while being turned by described Pipettor may be selected and a reagent chamber or the amplification chamber described in dynamic selecting hole；

Seal, the seal are sealedly attached to the pipettor close to the open end of the sealing cover, pass through the sealing Body is movably set with piston rod, and the piston rod connect with the liquid relief piston and is used to drive the liquid relief piston activity.

2. a kind of device of extraction purification nucleic acid according to claim 1, which is characterized in that the outer wall of the pipettor is straight Diameter is less than the aperture in the liquid relief hole, and the size of the outer diameter of the rotor is equal in magnitude with the aperture in the liquid relief hole.

3. a kind of device of extraction purification nucleic acid according to claim 1 or 2, which is characterized in that in the liquid relief body also It is provided with the second liquid relief portion, the diameter in second liquid relief portion is less than the diameter in first liquid relief portion, second liquid relief portion In be movably set with detection piston, be flexibly connected across the liquid relief lid and with the liquid relief lid and be useful for driving the detection to live Movable detection bar is filled in, detection hole, the detection hole and institute are offered on the liquid relief body in second liquid relief portion Detection inflow channel or reaction channel activity cooperation are stated, the liquid relief, which covers, is further opened with third air channel and the 4th ventilation Slot, rotatably selection is connected to the third air channel with the venting channels, passes through the third air channel and the ventilation Second liquid relief portion described in channel may be selected with an amplification chamber, the both ends of the 4th air channel are respectively with described the Two liquid relief portions and the connection of the space of the liquid relief body between second liquid relief portion and first liquid relief portion.

4. a kind of device of extraction purification nucleic acid according to claim 1, which is characterized in that the liquid relief body and the shifting It is provided with sealing rubber pad between sap cavity room, offers on the sealing rubber pad and becomes a mandarin with the reaction channel, the detection Channel and the matched sealing channel of detection outlet passageway.

5. a kind of device of extraction purification nucleic acid according to claim 1, which is characterized in that the reagent chamber includes to add Hot chamber, for being heated to sample.

6. a kind of device of extraction purification nucleic acid according to claim 5, which is characterized in that offer and add on the shell Heat channel, the heating chamber are located in the heating tank.

7. a kind of device of extraction purification nucleic acid according to claim 1, which is characterized in that the amplification chamber is provided with Several.