CN209836152U - Micro-fluidic chip for multi-channel fluorescent quantitative PCR amplification - Google Patents
Micro-fluidic chip for multi-channel fluorescent quantitative PCR amplification Download PDFInfo
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- CN209836152U CN209836152U CN201920430084.0U CN201920430084U CN209836152U CN 209836152 U CN209836152 U CN 209836152U CN 201920430084 U CN201920430084 U CN 201920430084U CN 209836152 U CN209836152 U CN 209836152U
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Abstract
The utility model discloses a micro-fluidic chip for multi-channel fluorescence quantitative PCR amplification, which comprises a sealing film, a reaction layer and a heat conduction layer from top to bottom; the reaction layer is a circular plate type heating block, a template sample adding hole, a plurality of micro channels and a plurality of reaction bins are arranged on the reaction layer, airflow holes are formed in the tops of the reaction bins, the reaction bins are communicated with the template sample adding hole through the micro channels, and the micro channels are capillaries; the plate sample adding hole, the micro flow channel and the reaction bin are embedded into the circular plate type heating block. The utility model discloses in be equipped with board-like circular heating piece, it is fast to go up and down the temperature, simultaneously the utility model discloses can realize the multiple detection of single sample, a application of sample, the multiple reaction hole is arrived in the automatic dispersion, can realize that the many targets of same sample detect simultaneously, and can not produce cross reaction.
Description
Technical Field
The invention belongs to the field of biomedical detection, and particularly relates to a micro-fluidic chip for multi-channel fluorescent quantitative PCR amplification.
Background
A microfluidic chip (microfluidic chip) is a scientific technology mainly characterized by the manipulation of fluids in a microscale space. As an emerging technology, the method is crossed with chemical, biological, engineering, physical and other subjects, and shows wide application prospect. The direct measurement of microorganism detection, particularly toxic microorganisms in food, has been a research hotspot of food microorganism detection, most of the existing microfluidic chips only analyze and detect single characteristics or a few characteristics of microorganisms, and cannot realize comprehensive analysis and detection of multiple characteristics of microorganisms, so that the detection efficiency is low.
SUMMERY OF THE UTILITY MODEL
Utility model purpose: in order to solve the problems that a PCR reaction tube is placed in a thicker heating module in the prior art, the temperature rising and falling speed is slow (2-3 ℃/s), the time consumption is long, and the simultaneous detection of multiple targets of the same sample is difficult to realize, the invention provides the microfluidic chip for the multichannel fluorescence quantitative PCR amplification.
The technical scheme is as follows: a micro-fluidic chip for multi-channel fluorescent quantitative PCR amplification comprises a sealing film, a reaction layer and a heat conduction layer from top to bottom;
the reaction layer is a circular plate type heating block, a template sample adding hole, a plurality of micro channels and a plurality of reaction bins are arranged on the reaction layer, airflow holes are formed in the tops of the reaction bins, the reaction bins are communicated with the template sample adding hole through the micro channels, and the micro channels are capillaries;
the plate sample adding hole, the micro flow channel and the reaction bin are embedded into the circular plate type heating block.
The reaction layer and the heat conduction layer are both made of thermosensitive materials, and rapid heating and rapid cooling can be achieved.
Adding the reaction liquid into the template sample adding hole, washing the reaction liquid into a reaction chamber through the capillary action of a micro-channel, pre-freezing the enzyme preparation in the reaction chamber, and then placing the chip on a PCR reaction instrument. The chip can be matched with a fluorescence quantitative PCR instrument matched with the chip developed by the applicant for use.
Wherein, the heat conduction layer is a heat conduction film.
Wherein, the sealing film is closely and detachably attached on the reaction layer.
The template sample adding holes are formed in the middle of the reaction layer, and the reaction bins are uniformly distributed around the template sample adding holes in a satellite shape.
The micro channels correspond to the reaction bins one by one, and the number of the reaction bins is more than or equal to 1.
Has the advantages that:
the utility model has the advantages as follows:
1. the utility model discloses a board-like circular heating piece (itself does not generate heat), board-like circular heating piece have increased the heated area of reaction liquid, make the temperature rise and fall fast (8 ~ 11 ℃/second).
2. The utility model discloses can realize the multiple detection of single sample, a application of sample, the multiple reaction storehouse of automatic dispersion can realize that the many targets of same sample detect simultaneously, and can not produce cross reaction.
3. The utility model discloses a micro-fluidic chip adopts the reaction liquid in the airtight reaction chip of pad pasting mode, stops the aerosol pollution, improves experiment security.
4. The utility model discloses can customize micro-fluidic detection chip according to the demand, make the type of freeze-drying in advance and detect the chip, can transport and preserve under normal atmospheric temperature.
Drawings
FIG. 1 is a schematic diagram of the structure of the multi-channel fluorescence quantitative PCR amplified microfluidic chip of the present invention.
FIG. 2 is a schematic diagram of the structure of the multi-channel fluorescence quantitative PCR amplified microfluidic chip of the present invention.
FIG. 3 is a schematic structural view of the reaction chamber.
In FIGS. 1 to 3, 1 is a reaction chamber, 2 is a microchannel, 3 is a template well, and 4 is an air flow hole.
Detailed Description
The invention will be better understood from the following examples. However, those skilled in the art will readily appreciate that the description of the embodiments is only for illustrating the present invention and should not be taken as limiting the invention as detailed in the claims.
A micro-fluidic chip for multi-channel fluorescent quantitative PCR amplification comprises a sealing film, a reaction layer and a heat conduction layer from top to bottom; the reaction layer is a circular plate type heating block, a template sample adding hole 3, a plurality of micro channels 4 and a plurality of reaction chambers 1 are arranged on the reaction layer, airflow holes 4 are arranged at the tops of the reaction chambers 1, the reaction chambers 1 are communicated with the template sample adding hole 3 through micro channels 2, and the micro channels 2 are capillaries; the plate sample adding hole 3, the micro flow channel 2 and the reaction chamber 1 are embedded into the circular plate type heating block.
Wherein, the heat conduction layer is a heat conduction film.
Wherein, the sealing film is closely and detachably attached on the reaction layer.
The template sample adding holes 3 are formed in the middle of the reaction layer, and the reaction chambers 1 are uniformly distributed around the template sample adding holes 3 in a satellite shape.
The micro channels 2 correspond to the reaction bins 1 one by one, and the number of the reaction bins 1 is more than or equal to 1.
The reaction layer and the heat conduction layer are both made of thermosensitive materials, and rapid heating and rapid cooling can be achieved.
Adding the reaction liquid into the template sample adding hole, washing the reaction liquid into a reaction chamber through the capillary action of a micro-channel, pre-freezing the enzyme preparation in the reaction chamber, and then placing the chip on a PCR reaction instrument. The chip can be matched with a fluorescence quantitative PCR instrument matched with the chip developed by the applicant for use.
Opening the sealing film, adding a proper amount of mixed sample and water into the template sample adding hole, enabling the sample and the water in the template sample adding hole to flow into each reaction bin by utilizing the capillary action, enabling a freeze-drying reagent (enzyme Mix) to be arranged in each reaction bin, attaching a film to a chip, carrying out on-machine detection, and analyzing results.
Claims (6)
1. A micro-fluidic chip for multi-channel fluorescent quantitative PCR amplification is characterized in that the chip comprises a sealing film, a reaction layer and a heat conduction layer from top to bottom;
the reaction layer is circular plate-type heating block, be equipped with template application of sample hole, a plurality of miniflow way, a plurality of reaction storehouse on the reaction layer, the reaction storehouse top is equipped with the air current hole, the reaction storehouse passes through miniflow way and template application of sample hole UNICOM, the miniflow way is the capillary.
2. The microfluidic chip for multi-channel fluorescence quantitative PCR amplification of claim 1, wherein the plate loading wells, the micro-channels, and the reaction chamber are embedded in a circular plate-type heating block.
3. The microfluidic chip for multi-channel fluorescent quantitative PCR amplification of claim 1, wherein the heat conducting layer is a heat conducting film.
4. The microfluidic chip for multi-channel fluorescence quantitative PCR amplification according to claim 1, wherein the sealing film is hermetically and detachably attached to the reaction layer.
5. The microfluidic chip for multi-channel fluorescence quantitative PCR amplification of claim 1, wherein the template sample adding hole is arranged in the middle of the reaction layer, and the reaction chambers are uniformly distributed around the template sample adding hole in a satellite shape.
6. The microfluidic chip for multi-channel fluorescence quantitative PCR amplification according to claim 1, wherein the micro-channels correspond to the reaction chambers one by one, and the number of the reaction chambers is greater than or equal to 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920430084.0U CN209836152U (en) | 2019-04-01 | 2019-04-01 | Micro-fluidic chip for multi-channel fluorescent quantitative PCR amplification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201920430084.0U CN209836152U (en) | 2019-04-01 | 2019-04-01 | Micro-fluidic chip for multi-channel fluorescent quantitative PCR amplification |
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| CN209836152U true CN209836152U (en) | 2019-12-24 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112782395A (en) * | 2020-12-30 | 2021-05-11 | 北京中科生仪科技有限公司 | PCR detector and method thereof |
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2019
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112782395A (en) * | 2020-12-30 | 2021-05-11 | 北京中科生仪科技有限公司 | PCR detector and method thereof |
| CN112782395B (en) * | 2020-12-30 | 2021-11-09 | 北京中科生仪科技有限公司 | PCR detector and method thereof |
| WO2022141674A1 (en) * | 2020-12-30 | 2022-07-07 | 北京中科生仪科技有限公司 | Pcr detector and method therefor |
| JP7438580B2 (en) | 2020-12-30 | 2024-02-27 | 北京中科生儀科技有限公司 | PCR detector and its method |
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