CN218098994U - Electrophoresis is with changeing low temperature auxiliary device for membrane - Google Patents
Electrophoresis is with changeing low temperature auxiliary device for membrane Download PDFInfo
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- CN218098994U CN218098994U CN202221926648.8U CN202221926648U CN218098994U CN 218098994 U CN218098994 U CN 218098994U CN 202221926648 U CN202221926648 U CN 202221926648U CN 218098994 U CN218098994 U CN 218098994U
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Abstract
The utility model relates to a low temperature auxiliary device for electrophoresis and film transfer, which comprises a low temperature box body, wherein a placing groove is arranged in the middle of the low temperature box body, a sealing cover is hinged at the notch of the placing groove, lifting components are symmetrically arranged at two sides in the placing groove, an operating platform for placing an electrophoresis groove is jointly and horizontally arranged between the lifting components, a central line is arranged at the center of the upper surface of the operating platform, limiting plates are arranged on the operating platforms at two sides of the central line in a sliding manner, and a perspective inner lattice is arranged at the front side of the low temperature box body corresponding to the position of the central line; and a liquid supplementing auxiliary area is also arranged on the low-temperature box body on one side of the placing groove. The utility model discloses novel structure, simple operation through set up lift operation platform in the low temperature box, have reduced the problem of taking repeatedly and removing of electrophoresis tank, and be provided with the fluid infusion auxiliary area in low temperature box one side, reagent and liquid feeding apparatus that use in can keeping in the experiment, liberation experimental space reduces the rate of utilization of experiment consumptive material.
Description
Technical Field
The utility model belongs to the technical field of the experimental device consumes, relate to Western Blot experimental apparatus, especially an electrophoresis and change low temperature auxiliary device for membrane.
Background
The Western Blot method is suitable for detecting proteins with the medium size of 1-5 ng, has very high sensitivity, is a relatively conventional analysis means in molecular biology, has various experimental steps from sample preparation, electrophoresis, gel cutting, membrane transfer and primary-antibody and secondary-antibody incubation to film development, and has certain influence on experimental results in each experimental step.
Electrophoresis and membrane transfer are two main experimental steps in Western Blot, a protein sample separated by PAGE transfers the protein to a solid phase carrier (such as a nitrocellulose film), and then is combined with a secondary immunochemical reagent to carry out luminescence detection, and for known expressed protein, a corresponding antibody can be used as a primary antibody to carry out detection, and for an expression product of a new gene, the antibody of a fusion part can be used for detecting.
At present, when Western Blot experiments are carried out in a laboratory, a vertical electrophoresis tank and a transfer tank are used, electrophoresis liquid and transfer liquid are placed in the middle, then the tank body is placed on ice for experiments, before sample adding, an electrophoresis internal tank body is placed on an experiment table, the electrophoresis liquid is added, after the sample adding, the internal tank body is placed in the electrophoresis tank, the electrophoresis tank is placed on an ice box for electrophoresis, the electrophoresis tank is repeatedly taken and moved in the operation process, the experiment operation space is occupied, complete stability cannot be guaranteed in the moving process, samples are dispersed in the electrophoresis liquid, and in the electrophoresis process, due to the fact that the low-temperature environment of the electrophoresis tank is uneven, electrophoresis bands are uneven, and the experiment results are influenced; and at the beginning of the experiment, the electrophoresis liquid needs to be added after the sample is added, if the electrophoresis liquid is directly added by a pipette, the sample can be flushed out of the sample hole and enters the electrophoresis liquid, the pipette needs to be replaced after multiple times of adding, the experiment consumables are wasted, and the experiment space is occupied. After the experiment, need retrieve to the liquid that uses, current experimental apparatus is unfavorable for the collection of liquid, can cause most liquid extravagant after using at every turn.
No patent literature relevant to the present application is found by search.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to overcome prior art's weak point, a novel structure is provided, the electrophoresis of simple operation and change low temperature auxiliary device for membrane, the device is through setting up the low temperature box of returning the font structure, realize low temperature, even electrophoresis and commentaries on classics membrane environment, simultaneously through setting up lift operation platform, the problem of the electrophoresis tank repeatedly taking and removing has been reduced, and be provided with the fluid infusion auxiliary area in low temperature box one side, reagent and the liquid feeding apparatus that uses in the experiment can be kept in, liberation experimental space, reduce the rate of utilization of experiment consumptive material.
The utility model provides a its technical problem take following technical scheme to realize:
a low-temperature auxiliary device for electrophoresis and film transfer comprises a low-temperature box body, wherein a placing groove is formed in the middle of the low-temperature box body, a sealing cover is hinged to the notch of the placing groove, lifting assemblies are symmetrically arranged on two sides in the placing groove, an operating platform for placing an electrophoresis groove is horizontally arranged between the lifting assemblies together, a center line is formed in the center of the upper surface of the operating platform, limiting plates are arranged on the operating platform on two sides of the center line in a sliding mode, and a perspective inner grid is formed in the front side of the low-temperature box body corresponding to the position of the center line; and a liquid supplementing auxiliary area is also arranged on the low-temperature box body on one side of the placing groove.
And, the fluid infusion auxiliary zone includes electrophoresis liquid temporary storage tank, waste liquid recovery tank and auxiliary instrument storage tank, electrophoresis liquid temporary storage tank is used for temporary storage electrophoresis liquid, and waste liquid recovery tank is used for carrying out recovery processing with the electrophoresis liquid that the electrophoresis was accomplished, and the auxiliary instrument storage tank is used for placing the pasteur straw.
Moreover, the operation platform comprises a supporting plate, a plurality of ventilation through holes are formed in the supporting plate, and scales are symmetrically formed on the surfaces of the two sides of the center line of the operation platform.
And an electrophoresis position indication line is also formed on the surface of the low-temperature box on one side of the perspective inner grid.
And the sealing cover is also provided with a power line extension port of the electrophoresis tank.
And the lifting assembly comprises a slide rail, an electric cylinder and a bracket, the slide rail is symmetrically arranged on two side walls in the placing groove, the electric cylinder is slidably arranged on the slide rail, the bracket is fixedly arranged on the electric cylinder through a support sheet, and the bracket is provided with an operating platform.
Moreover, the low-temperature box body is a hollow box body, and ice bags can be filled in the hollow box body or low-temperature liquid can be formed by flushing the ice bags into the hollow box body, so that the low-temperature box body shaped like a Chinese character 'hui' is formed.
The utility model has the advantages that:
the utility model has novel structure and convenient operation, provides a low-temperature and uniform electrophoresis and film transferring environment by arranging the low-temperature box body with the square-shaped structure, solves the problem that the electrophoresis strip of the sample is not straight in the electrophoresis process, and avoids the condition of film scorching in the film transferring process;
the utility model reduces the problems of repeated taking and moving of the electrophoresis tank by arranging the lifting operation platform, solves the problems that the sample is dispersed in the electrophoresis liquid because complete stability cannot be ensured in the moving process while the experiment operation space is occupied;
the utility model discloses still be provided with the fluid infusion auxiliary area in low temperature box one side, reagent and liquid feeding apparatus that use in can keeping in the experiment liberate the experimental space, reduce the rate of utilization of experiment consumptive material.
Drawings
FIG. 1 is a schematic view of the present invention;
fig. 2 is an explosion diagram of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the following embodiments, which are illustrative, not restrictive, and the scope of the invention should not be limited thereto.
A low-temperature auxiliary device for electrophoresis and film transfer comprises a low-temperature box body 5, wherein a placing groove 2 is formed in the middle of the low-temperature box body, a sealing cover 3 is hinged to the notch of the placing groove, lifting assemblies 1 are symmetrically arranged on two sides in the placing groove, an operation platform 4 used for placing an electrophoresis groove is jointly and horizontally arranged between the lifting assemblies, a central line 4-3 is formed in the center of the upper surface of the operation platform, limiting plates 4-2 are further arranged on the operation platforms on two sides of the central line in a sliding mode, the limiting plates are used for limiting the electrophoresis groove to be arranged in the middle of the operation platform, a perspective inner grid 8 is further formed in the front side of the box body corresponding to the position of the central line, the position of electrophoresis in the electrophoresis groove can be conveniently and clearly observed by an experimenter, the experimenter can conveniently monitor electrophoresis dynamic at any time, electrophoresis speed and electrophoresis time can be effectively controlled, and a sufficient experiment operation space can be ensured; a liquid supplementing auxiliary area 6 is further arranged on the box body on one side of the placing groove, so that the auxiliary use of experiments is facilitated;
the liquid supplementing auxiliary area comprises an electrophoretic liquid temporary storage tank, a waste liquid recovery tank and an auxiliary instrument storage tank, wherein the electrophoretic liquid temporary storage tank is used for temporarily storing electrophoretic liquid, the waste liquid recovery tank is used for recovering the electrophoretic liquid after electrophoresis, and the auxiliary instrument storage tank is used for placing liquid supplementing suction pipes such as Pasteur suction pipes and the like, so that the electrophoretic liquid can be supplemented at any time, the electrophoretic liquid can be supplemented at any time in the experiment process, the experiment space is liberated, and the experiment consumables are saved;
the operating platform comprises a supporting plate 4-1, a plurality of ventilation through holes 4-4 are formed in the supporting plate, so that cold air can conveniently pass through the supporting plate to be in contact with the bottom surface of the electrophoresis tank, and scales are symmetrically formed on the surfaces of the two sides of the center line of the operating platform and used for limiting the electrophoresis tank symmetrically so as to realize that the center point of the electrophoresis tank is located at the center line of the supporting plate;
the height of the perspective inner grid is higher than that of the maximum electrophoresis tank size, and an electrophoresis position indication line 7 is further arranged on the surface of the box body on one side of the perspective inner grid and can be used for observing the position of electrophoresis of a sample, so that the electrophoresis time can be conveniently estimated; the sealing cover is also provided with a power line extension port 3-1 of the electrophoresis tank;
the lifting assembly comprises sliding rails 1-1, electric cylinders 1-2 and brackets 1-3, the sliding rails are symmetrically arranged on two side walls in the placing groove, the electric cylinders are slidably arranged on the sliding rails, the brackets are fixedly arranged on the electric cylinders through supporting sheets 1-4, and operating platforms are arranged on the brackets; when the operation platform rises to be flush with the upper surface of the low-temperature box body, the electrophoresis tank is directly placed on the operation platform, the center line of the electrophoresis tank is positioned through the limiting plate, and then electrophoresis liquid is flushed in the electrophoresis tank and the like; then after the electrophoresis experiment is ready, the electric cylinder is driven to descend, the electrophoresis tank completely enters the placing groove, the power line of the electrophoresis tank extends out of the extending opening of the sealing cover, then the sealing cover is tightly covered, the power line is electrified, electrophoresis is carried out in a low-temperature environment, repeated taking and moving of the electrophoresis tank are avoided, sample dispersion in the unstable moving process is avoided, experiment operation links are reduced, and experiment efficiency is improved;
the low temperature box is the cavity box, can fill the ice bag in the cavity box or wash into and can form microthermal liquid, form a font low temperature box that returns.
The utility model discloses during specific operation:
when Western Blot electrophoresis is carried out, firstly, an ice bag is placed in the low-temperature box, or low-temperature liquid is introduced, then the electrophoretic liquid is placed in an electrophoretic liquid temporary storage groove of the liquid supplementing auxiliary area, a liquid supplementing pipe is convenient to be well arranged at the later stage, a sealing cover is opened, an operation platform is lifted to be parallel and level with the upper surface of the low-temperature box, the electrophoretic tank is directly placed on the operation platform, the electrophoretic tank is subjected to central line positioning through a limiting plate, and then the operations such as flushing the electrophoretic liquid are carried out; then, after the electrophoresis experiment is ready, driving the electric cylinder to descend to enable the electrophoresis tank to completely enter the placing groove, enabling a power line of the electrophoresis tank to extend out of an extending opening of the sealing cover, then tightly covering the sealing cover, and electrifying the power line to carry out the electrophoresis experiment; the position of electrophoresis is observed at any time through the electrophoresis position indicating line, and in the experimental process, if it is not enough to observe the electrophoresis liquid, the electrophoresis liquid is supplemented at any time by a liquid supplementing pipe, and after the electrophoresis is finished, the waste liquid can be poured into a waste liquid recovery tank, and then the electrophoresis experiment is carried out.
The utility model has novel structure and convenient operation, provides a low-temperature and uniform electrophoresis and film transferring environment by arranging the low-temperature box body with the square-shaped structure, solves the problem that the electrophoresis strip of the sample is not straight in the electrophoresis process, and avoids the condition of film scorching in the film transferring process;
the utility model reduces the problems of repeated taking and moving of the electrophoresis tank by arranging the lifting operation platform, solves the problem that the sample is dispersed in the electrophoresis liquid because the complete stability cannot be ensured in the moving process when the experiment operation space is occupied;
the utility model discloses still be provided with the fluid infusion auxiliary area in low temperature box one side, reagent and the liquid feeding apparatus that use in can keeping in the experiment, liberation experimental space reduces the rate of utilization of experiment consumptive material.
Although the embodiments of the present invention and the accompanying drawings are disclosed for illustrative purposes, those skilled in the art will appreciate that: various substitutions, changes and modifications are possible without departing from the spirit and scope of the invention and appended claims, and therefore, the scope of the invention is not limited to the disclosure of the embodiments and drawings.
Claims (7)
1. The utility model provides an electrophoresis and change low temperature auxiliary device for membrane which characterized in that: the electrophoresis tank comprises a low-temperature tank body, wherein a placing groove is formed in the middle of the low-temperature tank body, a sealing cover is hinged at the notch of the placing groove, lifting assemblies are symmetrically arranged on two sides in the placing groove, an operation platform for placing an electrophoresis tank is jointly and horizontally arranged between the lifting assemblies, a central line is formed in the center of the upper surface of the operation platform, limiting plates are further arranged on the operation platforms on two sides of the central line in a sliding mode, and a perspective inner grid is further formed in the front side of the low-temperature tank body corresponding to the position of the central line; and a liquid supplementing auxiliary area is also arranged on the low-temperature box body on one side of the placing groove.
2. The cryo-assisted apparatus for electrophoresis and membrane transfer of claim 1, wherein: the fluid infusion auxiliary area comprises an electrophoretic fluid temporary storage tank, a waste fluid recovery tank and an auxiliary appliance storage tank, wherein the electrophoretic fluid temporary storage tank is used for temporarily storing electrophoretic fluid, the waste fluid recovery tank is used for recovering the electrophoretic fluid after electrophoresis, and the auxiliary appliance storage tank is used for placing a Pasteur straw.
3. The cryo-assisted apparatus for electrophoresis and membrane transfer of claim 1, wherein: the operation platform comprises a support plate, wherein a plurality of ventilation through holes are formed in the support plate, and scales are symmetrically formed on the surfaces of two sides of the center line of the operation platform.
4. The low temperature auxiliary device for electrophoresis and transfer film according to claim 1, wherein: and an electrophoresis position indicating line is also arranged on the surface of the low-temperature box at one side of the perspective inner grid.
5. The low temperature auxiliary device for electrophoresis and transfer film according to claim 1, wherein: and the sealing cover is also provided with a power line extension port of the electrophoresis tank.
6. The low temperature auxiliary device for electrophoresis and transfer film according to claim 1, wherein: the lifting assembly comprises slide rails, an electric cylinder and a bracket, the slide rails are symmetrically arranged on two inner side walls of the placing groove, the electric cylinder is slidably arranged on the slide rails, the bracket is fixedly arranged on the electric cylinder through a supporting sheet, and an operating platform is arranged on the bracket.
7. The cryo-assisted apparatus for electrophoresis and membrane transfer of claim 1, wherein: the low temperature box body is a hollow box body, ice bags can be filled in the hollow box body or low-temperature liquid can be formed by flushing the ice bags into the hollow box body, and a low temperature box body shaped like a Chinese character 'hui' is formed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221926648.8U CN218098994U (en) | 2022-07-25 | 2022-07-25 | Electrophoresis is with changeing low temperature auxiliary device for membrane |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221926648.8U CN218098994U (en) | 2022-07-25 | 2022-07-25 | Electrophoresis is with changeing low temperature auxiliary device for membrane |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218098994U true CN218098994U (en) | 2022-12-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221926648.8U Active CN218098994U (en) | 2022-07-25 | 2022-07-25 | Electrophoresis is with changeing low temperature auxiliary device for membrane |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218098994U (en) |
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2022
- 2022-07-25 CN CN202221926648.8U patent/CN218098994U/en active Active
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