CN214431381U - Cell cryopreservation and reinfusion integrated cell therapy bag - Google Patents
Cell cryopreservation and reinfusion integrated cell therapy bag Download PDFInfo
- Publication number
- CN214431381U CN214431381U CN202120437783.5U CN202120437783U CN214431381U CN 214431381 U CN214431381 U CN 214431381U CN 202120437783 U CN202120437783 U CN 202120437783U CN 214431381 U CN214431381 U CN 214431381U
- Authority
- CN
- China
- Prior art keywords
- cell
- bag
- main body
- branch conduit
- space
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The utility model relates to a cell freezing and back-transfusion integrated cell therapy bag, which comprises a main body back-transfusion bag and a cell freezing inner container, wherein the main body back-transfusion bag is of a sealed bag structure; the upper part of the main body feedback space is a cell freezing space, and the volume of the cell freezing space is one third of the main body feedback space of the main body feedback bag; the lower end of the main body feedback bag is provided with a second branch catheter and a third branch catheter in an arrangement manner, and the cell cryopreservation space is connected with the first branch catheter; one end of the first branch conduit is connected with the cell cryopreservation space, and the other end of the first branch conduit is a convex port; one end of the second branch conduit is connected with the main body feedback bag, and the other end of the second branch conduit is a convex port; one end of the third branch conduit is connected with the main body feedback bag, and the other end of the third branch conduit is an output interface. The utility model has the advantages of simple and reasonable structure, convenient to use can avoid the pollution probability that frequent manual operation caused in cell cryopreserving, recovery, the feedback process, safety and sanitation, excellent in use effect.
Description
Technical Field
The utility model belongs to the technical field of biotechnology cell cryopreservation container, specifically relate to a cell cryopreservation and integrative cell therapy bag of reinfusion.
Background
The cell therapy is that some cells with specific functions of an organism are obtained by adopting a bioengineering method, and after the cells are treated by in vitro amplification, special culture and the like, the cells have the treatment effects of enhancing immunity, killing pathogens and tumor cells, promoting regeneration of tissues and organs, rehabilitating the organism and the like, thereby achieving the purpose of treating diseases. With the development of biological technologies, cell therapy is rapidly developing and increasingly accepted worldwide. The basis of cell therapy is the harvesting and storage of viable cells. Cell cryopreservation is one of routine work of cell culture, and is characterized in that a cryopreservation technology is utilized to place cells in a low-temperature environment, so that the cell metabolic rate is reduced, the cells are temporarily separated from a growth state to store the biological characteristics of the cells, and the cells are recovered when needed. The frozen cells can be returned to the human body after being recovered, and a cell return treatment bag is required to be applied. The sterile operation is strictly executed in the processes from freezing, recovery and final feedback of the cells, the existing operation process is complicated, and the feedback bags cannot meet the requirements of freezing, recovery, cell feedback, sterility guarantee and the like.
Disclosure of Invention
The utility model discloses to above-mentioned problem, compensate prior art not enough, provide a novel cell cryopreserved and integrative cell therapy bag of feedback, through the structure that changes the cell therapy bag, including addding the cell cryopreserved space, satisfy the cell cryopreserved, come back to life or life back the demand simultaneously.
In order to achieve the above purpose, the utility model adopts the following technical scheme.
The utility model relates to a cell cryopreserving and integrative cell therapy bag of reinfusion, its characterized in that: the cell cryopreservation device comprises a main body feedback bag and a cell cryopreservation inner container, wherein the main body feedback bag is of a sealed bag structure; the upper part of the main body feedback space is a cell freezing space, and the volume of the cell freezing space is one third of the main body feedback space of the main body feedback bag; the lower end of the main body feedback bag is provided with a second branch catheter and a third branch catheter in an arrangement mode, and the cell freezing space is connected with the first branch catheter.
As a preferred scheme of the utility model, one end of the first branch conduit is connected with the cell freezing space, and the other end is a convex port; one end of the second branch conduit is connected with the main body feedback bag, and the other end of the second branch conduit is a convex port; one end of the third branch conduit is connected with the main body feedback bag, and the other end of the third branch conduit is an output interface.
As another preferred scheme of the utility model be provided with the fixed hole that hangs in the middle of the top of main part feedback bag, the fixed hole that hangs is located the bag body edge of main part feedback bag.
As another preferable embodiment of the present invention, the first branch conduit, the second branch conduit and the third branch conduit are all plastic hoses.
As another preferred embodiment of the present invention, the convex ports of the first branch conduit and the second branch conduit are sealed by screw caps.
As another preferred scheme of the utility model, install the plastic-aluminum lid on the output interface of third branch pipe.
The utility model has the advantages of.
1. The utility model provides a cell cryopreserving and integrative cell therapy bag of feedback, simple structure is reasonable, and convenient to use can avoid the pollution probability that frequent manual operation caused at cell cryopreserving, recovery, feedback in-process, safety and sanitation, excellent in use effect.
2. The utility model discloses an integrative cell therapy bag is applicable to the live cell feedback system that medical staff operated and used under the ordinary environment and includes cell preparation at least, still is applicable to cell preparation's cell cryopreserving and feedback.
Drawings
In order to make the technical problem, technical scheme and beneficial effect that the utility model solved more clearly understand, it is right to combine the attached drawing and detailed description mode below the utility model discloses further detailed description. It should be understood that the detailed description and specific examples, while indicating the invention, are given by way of illustration only.
Fig. 1 is a schematic structural diagram of the cell cryopreservation and reinfusion integrated cell therapy bag of the present invention.
The labels in the figure are: 1 is a main body feedback space; 2 is a cell freezing space; 3 is a fixed hanging hole; 4 is a first branch conduit; 5 is a second branch conduit; and 6 is a third branch conduit.
Detailed Description
With reference to fig. 1, the cell cryopreservation and reinfusion integrated cell therapy bag of the present invention comprises a main body reinfusion space 1 and a cell cryopreservation space 2, which are integrally in a sealed bag structure; the lower part of the main body feedback space 1 is provided with a second branch conduit 5 and a third branch conduit 6, and the side wall of the cell freezing space 2 is provided with a first branch conduit 4; the volume of the cell freezing space 2 is one third of the volume of the main body feedback space 1, and the first branch conduit 4, the second branch conduit 5 and the third branch conduit 6 are plastic hoses; the first branch conduit 4 is connected with the cell freezing space 2, one end of the second branch conduit 5 is connected with the main body feedback space 1, and the other end is a convex port; the convex port of the second branch conduit 5 can be sealed by a screw cap, one end of the third branch conduit 6 is connected with the main body feedback space 1, the other end is an output interface, and an aluminum plastic cover is arranged on the output interface of the third branch conduit 6.
In order to improve the convenience of use, a fixed hanging hole 3 for fixing is arranged in the middle of the top end of the cell therapy bag; the fixed hanging hole 3 is positioned at the edge of the main body treatment bag.
Taking collection of amniotic mesenchymal stem cells as an example, the utility model is described in the following application:
1. and (3) sucking out the old culture solution after the fusion rate of the amnion mesenchymal stem cells cultured by adherence is observed under a microscope to reach 85-90%. Sodium chloride injection washes former blake bottle.
2. Digestion: the cells were digested with cell digesting enzymes until the cells were mostly changed from spindle to round and dropped under an inverted microscope, and the digestion was stopped by adding a culture medium.
3. Gently patting the culture bottle, collecting cells into a 50ml centrifuge tube, adding sodium chloride injection into the original culture bottle for washing, and centrifuging and washing.
4. Removing supernatant, adding sodium chloride injection, suspending cells, and centrifuging. And repeating the steps once.
5. The supernatant was removed, added with sodium chloride injection, suspended cells, filtered through a 100um sieve and centrifuged.
6. And (3) sucking and removing the supernatant, adding the cell cryopreservation liquid, uniformly mixing, opening the screw cap of the first branch catheter 4 of the cell therapy bag, connecting an outer tube of the syringe, pouring the cell suspension into the cell cryopreservation space 2 through the outer tube of the syringe, and sealing the first branch catheter 4 through a hot sealing machine. And storing the whole treatment bag in a liquid nitrogen tank for freezing storage.
7. When the clinical need to resuscitate the cells for the back transfusion therapy. The whole treatment bag is put into warm water for rapid resuscitation.
8. The screw cap of the second branch catheter 5 of the cell transfusion bag is opened, the outer tube of the injector is connected, the normal saline is poured into the main body transfusion space 1 through the outer tube of the injector and flows into the cell freezing space 2, the normal saline and the cell freezing space are fully mixed, and the second branch catheter 4 is sealed through a hot sealing machine. The cell therapy bag is attached with a cell coding label.
9. The cell therapy bag is filled into a vaccine box and sent out for clinical research in hospitals, and a blood transfusion leather strap is inserted from the output port of the third branch catheter 6 for back transfusion.
Through the above-mentioned specific embodiment, the beneficial effects of the utility model are that: the utility model has the advantages of simple and reasonable structure, convenient to use can avoid the pollution probability that frequent manual operation caused in cell collection, transportation, the testing process, and safety and sanitation, excellent in use effect.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (6)
1. A cell cryopreservation and reinfusion integrated cell therapy bag is characterized in that: the cell cryopreservation device comprises a main body feedback bag and a cell cryopreservation inner container, wherein the main body feedback bag is of a sealed bag structure; the upper part of the main body feedback space is a cell freezing space, and the volume of the cell freezing space is one third of the main body feedback space of the main body feedback bag; the lower end of the main body feedback bag is provided with a second branch catheter and a third branch catheter in an arrangement mode, and the cell freezing space is connected with the first branch catheter.
2. The integrated cell therapy bag for cell freezing and transfusion according to claim 1, wherein: one end of the first branch conduit is connected with the cell cryopreservation space, and the other end of the first branch conduit is a convex port; one end of the second branch conduit is connected with the main body feedback bag, and the other end of the second branch conduit is a convex port; one end of the third branch conduit is connected with the main body feedback bag, and the other end of the third branch conduit is an output interface.
3. The integrated cell therapy bag for cell freezing and transfusion according to claim 1, wherein: and a fixed hanging hole is formed in the middle of the top end of the main body feedback bag and is positioned on the edge of the bag body edge of the main body feedback bag.
4. The integrated cell therapy bag for cell freezing and transfusion according to claim 1, wherein: the first branch conduit, the second branch conduit and the third branch conduit are all plastic hoses.
5. The integrated cell therapy bag for cell freezing and transfusion according to claim 2, wherein: the convex ports of the first branch conduit and the second branch conduit are sealed by screw caps.
6. The integrated cell therapy bag for cell freezing and transfusion according to claim 2, wherein: and an aluminum-plastic cover is arranged on an output interface of the third branch conduit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120437783.5U CN214431381U (en) | 2021-03-01 | 2021-03-01 | Cell cryopreservation and reinfusion integrated cell therapy bag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120437783.5U CN214431381U (en) | 2021-03-01 | 2021-03-01 | Cell cryopreservation and reinfusion integrated cell therapy bag |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214431381U true CN214431381U (en) | 2021-10-22 |
Family
ID=78146882
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120437783.5U Active CN214431381U (en) | 2021-03-01 | 2021-03-01 | Cell cryopreservation and reinfusion integrated cell therapy bag |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214431381U (en) |
-
2021
- 2021-03-01 CN CN202120437783.5U patent/CN214431381U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9988599B2 (en) | Modular single-use kits and methods for preparation of biological material | |
| CN106479869A (en) | A kind of cell filter | |
| CN115226706A (en) | Cell cryopreservation liquid and cell cryopreservation and/or reinfusion method | |
| US20150328388A1 (en) | Device for separating/housing blood components and method for preparing platelet-rich plasma | |
| CN103484365B (en) | A kind of devices and methods therefor obtaining stem cell from tissue | |
| CN214431381U (en) | Cell cryopreservation and reinfusion integrated cell therapy bag | |
| CN105438655A (en) | Low-temperature granular fat collecting device for fat transplantation | |
| CN207193305U (en) | Doughnut exchanger and doughnut switch type culture systems | |
| AU2012229889A1 (en) | Cell processing method and device | |
| CN209890602U (en) | Novel cell feedback bag | |
| CN207452106U (en) | For the fat-derived primary stem cell collection piece-rate system of binary channels of human body liposuction | |
| CN212466737U (en) | Stem cell feedback device | |
| CN205071974U (en) | Portable liver normal temperature preservation device | |
| CN206328381U (en) | A kind of cell filter | |
| CN2647259Y (en) | Blood recovery device | |
| CN205441413U (en) | A granule fat low temperature collection device for fat transplantation | |
| CN108085254A (en) | Tissue engineering product culture apparatus | |
| CN106076442B (en) | Centrifugation bottle for PRP multistage parallel extraction, and use method thereof | |
| CN216018777U (en) | Special aseptic collection transportation processing bag of navel cord | |
| CN209630181U (en) | A kind of cell feedback bag and cell feedback system suitable for cell preparation | |
| CN214967595U (en) | A fill silicone tube for cataract surgery | |
| CN203530327U (en) | Device for acquiring dry cells from tissues | |
| CN214881585U (en) | Cell induction culture bag and NK cell induction culture bag | |
| CN217673947U (en) | Amnion tissue preservation and transportation bottle for human amnion stem cell culture | |
| CN201208415Y (en) | Self-body blood recovery device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |