CN209890602U - Novel cell feedback bag - Google Patents
Novel cell feedback bag Download PDFInfo
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- CN209890602U CN209890602U CN201821597363.8U CN201821597363U CN209890602U CN 209890602 U CN209890602 U CN 209890602U CN 201821597363 U CN201821597363 U CN 201821597363U CN 209890602 U CN209890602 U CN 209890602U
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Abstract
The utility model discloses a novel cell feedback bag, which comprises a main body feedback bag and an auxiliary bag for detection; the main body feedback bag is of a sealed bag body structure; the lower part of the main body feedback bag is communicated with a detection auxiliary bag through a first branch conduit; the detection auxiliary bag is of a sealed bag body structure; the volume of the auxiliary bag for detection is one twentieth to one eighth of the volume of the main body feedback bag; the lower end of the main body feedback bag is provided with a second branch conduit and a third branch conduit in an arrangement way; the first branch conduit, the second branch conduit and the third branch conduit are all plastic hoses; one end of the second branch conduit is connected with the main body feedback bag, and the other end is a convex port; one end of the third branch conduit is connected with the main body feedback bag, and the other end is an output interface. The utility model has the advantages of simple and reasonable structure, convenient to use can avoid the pollution probability that frequent manual operation caused in cell collection, transportation, the testing process, and safety and sanitation, excellent in use effect.
Description
Technical Field
The utility model relates to a biological assay apparatus field, in particular to novel cell feedback bag.
Background
In recent years, on the basis of rapid development of cell biology, particularly stem cell biology, cell therapy has begun to be applied to clinical experimental research on various diseases such as cardiovascular system diseases, nervous system diseases, immune system diseases, diabetes, cancers and the like, and exciting primary research results are obtained, so that a wide clinical application prospect is shown. The cell therapy is that some cells with specific functions of an organism are obtained by adopting a bioengineering method, and after the cells are treated by in vitro amplification, special culture and the like, the cells have the treatment effects of enhancing immunity, killing pathogens and tumor cells, promoting regeneration of tissues and organs, rehabilitating the organism and the like, thereby achieving the purpose of treating diseases. With the development of biological technologies, cell therapy is rapidly developing and increasingly accepted worldwide. The basis of cell therapy is the harvesting and storage of viable cells. A reinfusion bag is a bag that stores cells for reinfusion into the body. In the cell treatment process, cells need to be stored in the same container from collection to final feedback, so that pollution is avoided, the biological sample may need to be detected before feedback, and the existing feedback bags cannot meet the requirements of collection, cell feedback, sterility guarantee, sampling detection and the like.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model provides a novel cell feedback bag, through the structure that changes cell feedback bag, including addding the detection with attaching the bag, solved current feedback bag and had the problem that all can't satisfy demands such as collection, cell feedback, aseptic guarantee, sampling test.
In order to achieve the above purpose, the technical scheme of the utility model is as follows: a novel cell feedback bag comprises a main body feedback bag and an auxiliary bag for detection; the main body feedback bag is of a sealed bag body structure; the lower part of the main body feedback bag is communicated with the detection auxiliary bag through a first branch conduit; the auxiliary detection bag is of a sealed bag body structure; the volume of the auxiliary bag for detection is one twentieth to one eighth of the volume of the main body feedback bag; a second branch conduit and a third branch conduit are arranged at the lower end of the main body feedback bag; the first branch conduit, the second branch conduit and the third branch conduit are all plastic hoses; one end of the second branch conduit is connected with the main body feedback bag, and the other end of the second branch conduit is a convex port; one end of the third branch conduit is connected with the main body feedback bag, and the other end of the third branch conduit is an output interface.
As a preferred scheme of the utility model, a fixed hanging port is arranged in the middle of the top end of the main body feedback bag; the fixed hanging port is positioned on the edge of the bag body of the main body feedback bag.
As a preferred aspect of the present invention, the male port of the second branch conduit is sealed by a screw cap.
As an optimized proposal of the utility model, an aluminum-plastic cover is arranged on the output interface of the third branch conduit.
As a preferable embodiment of the present invention, the volume of the detection accessory bag is one twelfth to one tenth of the volume of the main body feedback bag.
Through the technical scheme, the utility model discloses technical scheme's beneficial effect is: the utility model has the advantages of simple and reasonable structure, convenient to use can avoid the pollution probability that frequent manual operation caused in cell collection, transportation, the testing process, and safety and sanitation, excellent in use effect.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic structural diagram of the present invention.
The corresponding part names indicated by the numbers and letters in the drawings:
1. main body feedback bag 2, detecting auxiliary bag 3, first branch conduit
4. A second branch conduit 5, a third branch conduit.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
Examples
With reference to fig. 1, the utility model discloses a novel cell feedback bag, which comprises a main body feedback bag 1 and a detection auxiliary bag 2. The main body feedback bag 1 is of a sealed bag structure. The detection bag 2 is connected to the lower part of the main body reinfusion bag 1 through a first branch conduit 3. The detection auxiliary bag 2 is of a sealed bag structure. In order to save the cell fluid and satisfy the requirement of the test, the volume of the test secondary bag 2 is one twentieth to one eighth of the volume of the main body return bag 1. Preferably, the volume of the detection accessory bag 2 is one twelfth to one tenth of the volume of the main body reinfusion bag 1. A second branch conduit 4 and a third branch conduit 5 are arranged at the lower end of the main body feedback bag 1. The first branch conduit 3, the second branch conduit 4 and the third branch conduit 5 are all plastic hoses; one end of the second branch conduit 4 is connected with the main body feedback bag 1, and the other end is a convex port. The male port of the second branch conduit 4 may be sealed by a screw cap. One end of the third branch conduit 5 is connected with the main body feedback bag 1, and the other end is an output interface. An aluminum-plastic cover is arranged on an output interface of the third branch conduit 5.
In order to improve the convenience of use, a fixed hanging opening is arranged in the middle of the top end of the main body feedback bag 1; the fixed hanging port is positioned on the edge of the bag body of the main body feedback bag 1.
Taking the collection of umbilical cord mesenchymal stem cells as an example, the utility model is illustrated in the use process:
1. and (3) sucking out the old culture solution after the fusion rate of the umbilical cord mesenchymal stem cells cultured by adherence is observed under a microscope to reach 85-90%. Sodium chloride injection washes former blake bottle.
2. Digestion: the cells were digested with cell digesting enzymes until the cells were mostly changed from spindle to round and dropped under an inverted microscope, and the digestion was stopped by adding a culture medium.
3. Gently tapping the culture bottle, collecting cells into a 50m l centrifuge tube, adding sodium chloride injection into the original culture bottle for washing, and centrifuging and washing.
4. Removing supernatant, adding sodium chloride injection, suspending cells, and centrifuging. Is repeated once
5. The supernatant was removed, added with sodium chloride injection, suspended cells, filtered through a 100um sieve and centrifuged.
6. And (3) sucking and removing the supernatant, adding cell release liquid, uniformly mixing, opening a screw cap of a second branch catheter 4 of the cell reinfusion bag, connecting an outer tube of the syringe, pouring the cell suspension into the main body reinfusion bag 1 through the outer tube of the syringe, flowing into the attached bag 2 for detection, and sealing the second branch catheter 4 through a hot sealing machine. The main bag and the detection tag bag 2 are labeled with cell-encoding labels.
7. Shaking the cell transfusion bag, heat-sealing the first branch conduit 3 with a heat sealing machine, cutting off the attached bag 2 for detection, storing the sample, and performing cell counting, endotoxin detection, sterility detection, etc.
8. The main body return transfusion bag 1 is arranged in a vaccine box and sent out for clinical research of hospitals, and a blood transfusion leather strap is inserted from an output port of the third branch catheter 5 for return transfusion.
Through the above-mentioned specific embodiment, the beneficial effects of the utility model are that: the utility model has the advantages of simple and reasonable structure, convenient to use can avoid the pollution probability that frequent manual operation caused in cell collection, transportation, the testing process, and safety and sanitation, excellent in use effect.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (1)
1. A novel cell feedback bag is characterized by comprising a main body feedback bag and an auxiliary bag for detection; the main body feedback bag is of a sealed bag body structure; the lower part of the main body feedback bag is communicated with the detection auxiliary bag through a first branch conduit; the auxiliary detection bag is of a sealed bag body structure; a second branch conduit and a third branch conduit are arranged at the lower end of the main body feedback bag; the first branch conduit, the second branch conduit and the third branch conduit are all plastic hoses; one end of the second branch conduit is connected with the main body feedback bag, and the other end of the second branch conduit is a convex port; one end of the third branch conduit is connected with the main body feedback bag, and the other end of the third branch conduit is an output interface; a fixed hanging opening is arranged in the middle of the top end of the main body feedback bag; the fixed hanging port is positioned on the edge of the bag body of the main body feedback bag; the male port of the second branch conduit is sealed by a screw cap; an aluminum-plastic cover is arranged on an output interface of the third branch conduit; the volume of the auxiliary bag for detection is one twelfth to one tenth of that of the main body feedback bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821597363.8U CN209890602U (en) | 2018-09-29 | 2018-09-29 | Novel cell feedback bag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821597363.8U CN209890602U (en) | 2018-09-29 | 2018-09-29 | Novel cell feedback bag |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209890602U true CN209890602U (en) | 2020-01-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821597363.8U Active CN209890602U (en) | 2018-09-29 | 2018-09-29 | Novel cell feedback bag |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209890602U (en) |
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2018
- 2018-09-29 CN CN201821597363.8U patent/CN209890602U/en active Active
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