CN203530327U - Device for acquiring dry cells from tissues - Google Patents
Device for acquiring dry cells from tissues Download PDFInfo
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- CN203530327U CN203530327U CN201320534151.6U CN201320534151U CN203530327U CN 203530327 U CN203530327 U CN 203530327U CN 201320534151 U CN201320534151 U CN 201320534151U CN 203530327 U CN203530327 U CN 203530327U
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- tissue
- stem cell
- digestion tank
- drain pipe
- reagent bottle
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Abstract
The utility model provides a device for acquiring dry cells from tissues. The device comprises a tissue digestion tank, a reagent bottle, a peristaltic pump and a collection device as well as a three-way valve, wherein the tissue digestion tank is connected with a liquid inlet of the three-way valve through a pipeline; the reagent bottle and the collection device are connected with two liquid inlets of the three-way valve through connecting pipes respectively; a liquid outlet pipe is arranged in the reagent bottle, is clamped on the peristaltic pump and is connected with the tissue digestion tank. The device is reasonable in structure, can be suitable for different tissue sources and different separation methods (through enzymatic digestion or non enzymatic digestion), and can be used for safely, conveniently, quickly and effectively obtaining the dry cells from the different tissues.
Description
Technical field
The utility model relates to a kind of cell extraction device, is specifically related to a kind of device that obtains stem cell from tissue.
Background technology
Stem cell is a class cell that has self-renewal capacity, can be divided under given conditions dissimilar functioning cell.Stem-cell research and application can break through the restriction of traditional medicine development, be expected to solve a significant medical difficult problem for facing mankind, now for transplantation treatment, nervous system disease, gene therapy, research fetal development and disease generation and drug screening and exploitation etc. provide feasible method.Adult stem cell can be taken from patient self, does not have the problem of histocompatibility when application, has avoided graft-rejection.
Investigator's discovery, stem cell can derive from numerous tissues, as marrow, fat, umbilical cord, placenta, decidua, amnion, amniotic fluid, skin, muscle, dental pulp, blood vessel, heart, spleen, perirenal fat, intestinal epithelial tissue etc.And the separation of stem cell mainly adopts the mode of organizing the one or more combination enzymic digestions such as Dispase through collagenase, trypsinase, neutrality, from tissue, isolate stem cell, carry out further cultivating amplification.Yet existing method for separating stem cell is all the mode based on experimental study, still also lack a set of completely, be applicable to various tissue-derived stem cell tripping devices.
Summary of the invention
In view of the deficiencies in the prior art, the purpose of this utility model be to provide a kind of from tissue device and the using method thereof of separate stem cells, this device rational in infrastructure in conjunction with this using method, can obtain stem cell safe, convenient, fast, effectively from different tissues.
Specific embodiments of the present utility model is: a kind of device that obtains stem cell from tissue, comprise tissue digestion tank, reagent bottle, peristaltic pump and collection device, the described device that obtains stem cell also includes T-valve, described tissue digestion tank is connected with T-valve fluid inlet by pipeline, described reagent bottle leads to take over collection device and is not connected with two liquid outlets of T-valve, in described reagent bottle, be also provided with drain pipe, described drain pipe is sandwiched on peristaltic pump and with tissue digestion tank and is connected.
Further, described drain pipe comprises leading portion drain pipe and back segment drain pipe, between described leading portion tubing and back segment tubing, by stainless steel elbow, is connected, and described stainless steel elbow is positioned in temperature-adjustable device (water-bath).
Further, described tissue digestion tank comprises tank body and the spiral cover that is placed on tank body top, described tank body opening part is provided with the first filter screen, described spiral cover is provided with the through hole for connecting tube, at the bottom of described tissue digestion tank tank, be provided with a magnetic force generator, on described magnetic force generator, be placed with several magnetic beads, on described tissue digestion pot sidewall, be also extended with the jack for being connected with back segment drain pipe.
Further, described collection device comprises a funnel-form bottle, and described bottle bottleneck is provided with the second filter screen, and bottle funnel-form sharp-crested place, the end is provided with opening, and described bottle below is provided with receiving flask.
Further, the pipe end that described drain pipe inserts reagent bottle is connected with and is inserted to minute hand pipe at the bottom of reagent bottle.
Further, described temperature-adjustable device is water-bath.
Further, between described tissue digestion tank and spiral cover, be also provided with the sealing-ring that prevents digester liquid leakage.
Further, described the first aperture of filter screen is 100～200 orders.
Further, described the second aperture of filter screen is 200～300 orders.
Further, described tissue digestion tank diameter 2～30cm.
Compared with prior art, the utlity model has following beneficial effect: should from tissue, the device of separate stem cells be sterilizable sterile device, each integral part is reasonable in design, the magnetic bead of tissue digestion tank and magnetic force generator can fully stir digestion, and the mechanical force separate mode of non-enzyme can be provided for tissue digestion.The stainless steel elbow having can hot digestion enzyme, and enzyme is played a role in optimum temperuture.Whole system can form digestion circulation loop, makes the stem cell digesting not need to rest on completely in digester, improves the activity of the stem cell having digested.And by the same direction of peristaltic pump, wriggling constantly to pressurize filters different filtering nets, reaches filtration residue tissue and isolated mononuclear cell effect.This device goes for different tissue sources, and different separation methods (through enzyme or without enzymic digestion), for clinical use stem cell provides a set of safety, aspect, effective separate stem cells device.
Accompanying drawing explanation
Fig. 1 is a kind of cell extraction apparatus structure of the utility model schematic diagram.
in figure: 1-tissue digestion tank, 2-reagent bottle, 3-peristaltic pump, 4-collection device, 5-T-valve, 6-drain pipe, 7-regulates temperature device, 8-receiving flask, 11-tank body, 12-spiral cover, 13-the first filter screen, 14-magnetic force generator, 15-magnetic bead, 16-jack, 21-minute hand pipe, 41-bottle, 42-the second filter screen, 63-stainless steel elbow, 61-leading portion drain pipe, 62-back segment drain pipe, 121-through hole.
Embodiment
Below in conjunction with the drawings and specific embodiments, the utility model is described in more detail.
As shown in Figure 1, a kind of device that obtains stem cell from tissue, comprise tissue digestion tank 1, reagent bottle 2, peristaltic pump 3 and collection device 4, described in obtain stem cell device also include T-valve 5, described tissue digestion tank 1 is connected with T-valve fluid inlet 51 by pipeline, described reagent bottle 2 leads to take over collection device and is not connected with two liquid outlets of T-valve 5, in described reagent bottle 2, is also provided with drain pipe 6, and described drain pipe 6 is sandwiched on peristaltic pump 3 and with tissue digestion tank 1 and is connected.
Wherein, described drain pipe 6 comprises leading portion drain pipe 61 and back segment drain pipe 62, between described leading portion tubing and back segment tubing, by stainless steel elbow 63, is connected, and described stainless steel curved 63 is positioned in temperature-adjustable device 7.This temperature-adjustable device 7 can be water-bath.
Described tissue digestion tank 1 comprises tank body 11 and the spiral cover 12 that is placed on tank body 11 tops, described tank body 11 opening parts are provided with the first filter screen 13, described spiral cover 12 is provided with the through hole 121 for connecting tube, at the bottom of described tissue digestion tank 1 tank, be provided with a magnetic force generator 14, on described magnetic force generator 14, be placed with several magnetic beads 15, on described tissue digestion tank 1 sidewall, be also extended with the jack 16 for being connected with back segment drain pipe 62.12 of tissue digestion tank 11 and spiral covers are also provided with the sealing-ring 17 that prevents digester liquid leakage.
The above-mentioned device that obtains stem cell from tissue forms and comprises digestion circulation loop and collection channel.
(1) digestion circulation loop mainly consists of tissue digestion tank 1, T-valve 5, reagent bottle 2, peristaltic pump 3.
Tissue digestion tank 1 is mainly used in putting into the tissue that extracts stem cell, can be designed to put into magnetic bead 15 and can fully stir digestion under the effect of magnetic force generator 14, for tissue digestion provides the mechanical force separate mode of non-enzyme in tank body 12.The magnetic force generator 14 of tissue digestion tank 11 bottoms can be an integral body with tissue digestion tank 1, can split be also isolated system.Magnetic force generator 14 by circuit control can be divided into soft, medium, strong, superpower several grades.This magnetic force generator can be also other device or the methods that can realize digester 1 concussion, as hand in employing vibrator etc.The diameter of tissue digestion tank 1 is 2～30cm, preferably has 3cm, 5cm, 8cm, 10cm, 15cm or 20cm.The aperture of the first filter screen 13 of described tank body 11 opening parts can be 100～200 orders, reaches filtration residue tissue and isolated mononuclear cell effect.
T-valve 5 is delivered to reagent bottle 2 or collection device for changing flow liquid.When T-valve 5 is threaded to while making state that tissue digestion tank 1 is connected with collection device 4, reagent bottle 2 cannot be communicated with tissue digestion tank 1, otherwise when T-valve 5 is threaded to while making state that tissue digestion tank 1 is connected with reagent bottle 2, collection device cannot be communicated with tissue digestion tank.
Reagent bottle 2 is for adding phosphate buffered saline buffer, physiological saline or as digestive ferments such as collagenase, trypsinase or mixed enzyme.To realize, the cell in tissue is decomposed or cleaned.In addition, reagent bottle 2 can direct replacement be reagent bag or normal saline bag.
The circulation that peristaltic pump 3 is liquid provides driving power.During use, peristaltic pump 3 is arranged between tissue digestion tank 1 and reagent bottle 2, and drain pipe is sandwiched in peristaltic pump 3, utilizes hocket extruding and discharge and to pump out fluid of peristaltic pump 3, realizes Weaving Cycle.
The mode of connection of digestion circulation loop: tissue digestion tank 1 is connected with T-valve fluid inlet by inserting the pipeline of spiral cover top through hole, a liquid outlet of T-valve is connected with reagent bottle 2 by pipe connecting, this pipe connecting can connect needle tubing at the tube end being connected with reagent bottle 2, to facilitate, inserts in reagent bottle 2.In reagent bottle 2, be also inserted with for getting rid of the liquid discharge pipe of reagent bottle 2 liquid, similarly, for convenient, insert in reagent bottle 2, also can connect and be inserted to 2 end of reagent bottle minute hand pipe 21 at tube end, described minute hand pipe diameter can be 22#, material is rigid plastics material or stainless steel (needle tubing needs to be furnished with block before using), and above-mentioned pipe connecting material is medical grade silicone pipe.
For enzyme is played a role in optimum temperuture, in the middle of described drain pipe, be connected with the stainless steel elbow 63 being placed in temperature control equipment 7, make drain pipe 6 be divided into leading portion drain pipe 61 and back segment drain pipe 62, described stainless steel elbow 63 is positioned in temperature-adjustable device 7 so that digestive ferment guarantees in optimum temperuture.
The digestion circulation loop forming, make the stem cell digesting not need to rest on completely in tissue digestion tank 1, improve the activity that has digested stem cell, and by the same direction of peristaltic pump 3, wriggling constantly to pressurize filters different filtering nets, reaches filtration residue tissue and isolated mononuclear cell effect.
(2) collection channel comprise tissue digestion tank 1, T-valve 5, with collection device 4, collection device 4 is connected by pipeline with a liquid outlet of T-valve 5, described collection device is a funnel-form bottle 41, described bottle bottleneck is provided with the second filter screen 42, and described the second filter screen 42 apertures are 200～300 orders.Bottle funnel-form sharp-crested place, the end is provided with opening, and described bottle below is provided with receiving flask 8.Funnel type bottle can facilitate cell harvesting, and described opening can be connected by pipeline with receiving flask, and described receiving flask can also be stem cell collecting bag.
The foregoing is only preferred embodiment of the present utility model, all equalizations of doing according to the utility model claim change and modify, and all should belong to covering scope of the present utility model.
Claims (10)
1. one kind is obtained the device of stem cell from tissue, it is characterized in that, comprise tissue digestion tank, reagent bottle, peristaltic pump and collection device, described in obtain stem cell device also include T-valve, described tissue digestion tank is connected with T-valve fluid inlet by pipeline, described reagent bottle leads to take over collection device and is not connected with two liquid outlets of T-valve, is also provided with drain pipe in described reagent bottle, and described drain pipe is sandwiched on peristaltic pump and with tissue digestion tank and is connected.
2. the device of stem cell of obtaining from tissue according to claim 1, it is characterized in that, described drain pipe comprises leading portion drain pipe and back segment drain pipe, between described leading portion tubing and back segment tubing, by stainless steel elbow, be connected, described stainless steel elbow is positioned in temperature-adjustable device.
3. the device of stem cell of obtaining from tissue according to claim 1, it is characterized in that, described tissue digestion tank comprises tank body and the spiral cover that is placed on tank body top, described tank body opening part is provided with the first filter screen, described spiral cover is provided with the through hole for connecting tube, at the bottom of described tissue digestion tank tank, be provided with a magnetic force generator, on described magnetic force generator, be placed with several magnetic beads, on described digester sidewall, be also extended with the jack for being connected with back segment drain pipe.
4. the device of stem cell of obtaining from tissue according to claim 1, it is characterized in that, described collection device comprises a funnel-form bottle, and in described bottle, bottleneck is provided with the second filter screen, bottle funnel-form sharp-crested place, the end is provided with opening, and described bottle below is provided with receiving flask.
5. according to claim 1ly from tissue, obtain the device of stem cell, it is characterized in that, the pipe end that described drain pipe inserts reagent bottle is connected with and is inserted to minute hand pipe at the bottom of reagent bottle.
6. the device that obtains stem cell from tissue according to claim 2, is characterized in that, described temperature-adjustable device is water-bath.
7. the device that obtains stem cell from tissue according to claim 3, is characterized in that, between described tissue digestion tank and spiral cover, is also provided with the sealing-ring that prevents digester liquid leakage.
8. the device that obtains stem cell from tissue according to claim 3, is characterized in that, described the first aperture of filter screen is 100～200 orders.
9. the device that obtains stem cell from tissue according to claim 4, is characterized in that, described the second aperture of filter screen is 200～300 orders.
10. the device that obtains stem cell from tissue according to claim 1, is characterized in that described tissue digestion tank diameter 2～30cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320534151.6U CN203530327U (en) | 2013-08-30 | 2013-08-30 | Device for acquiring dry cells from tissues |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320534151.6U CN203530327U (en) | 2013-08-30 | 2013-08-30 | Device for acquiring dry cells from tissues |
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| Publication Number | Publication Date |
|---|---|
| CN203530327U true CN203530327U (en) | 2014-04-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201320534151.6U Expired - Fee Related CN203530327U (en) | 2013-08-30 | 2013-08-30 | Device for acquiring dry cells from tissues |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103484365A (en) * | 2013-08-30 | 2014-01-01 | 中国人民解放军南京军区福州总医院 | Device and method for obtaining stem cells from tissues |
-
2013
- 2013-08-30 CN CN201320534151.6U patent/CN203530327U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103484365A (en) * | 2013-08-30 | 2014-01-01 | 中国人民解放军南京军区福州总医院 | Device and method for obtaining stem cells from tissues |
| CN103484365B (en) * | 2013-08-30 | 2015-09-16 | 中国人民解放军南京军区福州总医院 | A kind of devices and methods therefor obtaining stem cell from tissue |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140409 Termination date: 20200830 |
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| CF01 | Termination of patent right due to non-payment of annual fee |