CN214174399U

CN214174399U - Multichannel sliding type fluorescence immunoassay device

Info

Publication number: CN214174399U
Application number: CN202023147995.4U
Authority: CN
Inventors: 陈渝峰; 邓苑佐
Original assignee: Chongqing Kang Ju Quan Hong Biotechnology Co ltd
Current assignee: Chongqing Kang Ju Quan Hong Biotechnology Co ltd
Priority date: 2020-12-23
Filing date: 2020-12-23
Publication date: 2021-09-10
Anticipated expiration: 2030-12-23

Abstract

The utility model relates to the field of immunoassay analyzer, and discloses a multichannel sliding type fluorescence immunoassay device, which comprises a shell, wherein a base is fixed in the shell, a plurality of inserting ports are arranged on the side part of the shell, a plurality of reagent clamping seats which are arranged in sequence and fixed with each other are connected on the base in a sliding way, and the reagent clamping seats are arranged corresponding to the inserting ports; a driving mechanism for driving the reagent clamping seat to slide is arranged in the shell, and a detection mechanism which can be just opposite to the reagent clamping seat is connected to the top of the shell in a sliding mode. The utility model provides a fluorescence immunoassay appearance among the prior art detect the problem that the continuity is poor, inefficiency.

Description

Multichannel sliding type fluorescence immunoassay device

Technical Field

The utility model relates to an immunoassay appearance field, concretely relates to multichannel slidingtype fluorescence immunoassay device.

Background

The biochemical analyzer is one of the common apparatuses for medical detection tests, and can perform analysis and measurement of various reaction types such as a timing method and a continuous monitoring method. The fluorescence immunoassay technology is an important detection and analysis technology in biochemical analysis technology, has the advantages of strong specificity, high sensitivity, good practicability and the like, and is used for measuring bioactive compounds with very low content, such as proteins (enzymes, receptors and antibodies), hormones (steroids, thyroid hormones and phthalein hormones), medicines, microorganisms and the like.

The fluorescence immunoassay analyzer is an apparatus for detecting by utilizing the fluorescence effect of a fluorescent substance, wherein a fluorescence element is arranged on an immunochromatography test strip (or a reagent card), and after the fluorescence element is irradiated by an excitation light source and absorbs incident light with a certain wavelength, the fluorescence with the wavelength slightly longer than that of the incident light can be emitted; the content of the corresponding antigen in the detected object can be obtained by converting the fluorescence intensity into an electric signal and analyzing the electric signal. Most fluorescence immunoassay appearance all is the single channel structure at present, inserts the back with the reagent card from the card slot when using, utilizes fluorescence detection probe to detect the strong and weak back of fluorescence, takes out the back with the reagent card, inserts next reagent card repeated detection again, and the continuity of detection is poor, and detection efficiency is lower, has increased operating personnel's work burden, can't satisfy the great test demand of sample size.

SUMMERY OF THE UTILITY MODEL

The utility model discloses it is anticipated to provide multichannel slidingtype fluorescence immunoassay device to solve the fluorescence immunoassay appearance among the prior art and detect the problem that the continuity is poor, inefficiency.

In order to achieve the above purpose, the utility model adopts the following technical scheme: the multichannel sliding type fluorescence immunoassay device comprises a shell, wherein a base is fixed in the shell, a plurality of inserting ports are formed in the side part of the shell, a plurality of reagent clamping seats which are sequentially arranged and mutually fixed are connected to the base in a sliding mode, and the reagent clamping seats and the inserting ports are arranged correspondingly; a driving mechanism for driving the reagent clamping seat to slide is arranged in the shell, and a detection mechanism which can be just opposite to the reagent clamping seat is connected to the top of the shell in a sliding mode.

The principle and the advantages of the scheme are as follows: during practical application, among this technical scheme, the casing plays the effect that whole was held, guarantees the stable connection between each part of equipment simultaneously, and the bayonet socket is used for inserting the reagent card that treats the measuring in the reagent cassette. The driving mechanism is used for driving the reagent card seat to slide, so that the reagent card seat drives the reagent card to be detected to move towards the detection mechanism and is positioned below the detection mechanism. Because the detection mechanism is connected in the shell in a sliding manner, the detection mechanism can slide along the shell and sequentially complete the fluorescence detection of a row of reagent cards to be detected. The technical scheme can realize the purposes of placing the detection cards in batches and detecting in batches, and improves the continuity of detection and the efficiency of detection.

Preferably, as an improvement, the detection mechanism includes a fluorescence detection assembly slidably attached to the housing and a drive assembly for driving the fluorescence detection assembly to slide intermittently.

In the technical scheme, under the driving action of the driving assembly, the fluorescence detection assembly intermittently slides along the shell, namely, the fluorescence detection assembly is in a sliding and static intermittent state, and when the fluorescence detection assembly detects the reagent card, the fluorescence detection assembly is in a static state; after the detection of one reagent card is finished, the fluorescence detection assembly slides to the upper part of the next reagent card to be detected under the driving action of the driving assembly to detect the next reagent card, and the structural design is reasonable.

Preferably, as an improvement, the driving assembly comprises a driving shaft rotatably connected to the top of the housing, the driving shaft is coaxially and fixedly connected with a driving disc, and the eccentric position of the driving disc is fixedly connected with a driving column; the top of casing still rotates and is connected with the pivot, and the coaxial rigid coupling of pivot has the carousel, is provided with a plurality of driving holes that can hold the drive post on the carousel, and the driving hole sets up along pivot circumference equipartition, and the casing internal rotation is connected with the auxiliary shaft, is connected with the drive belt between auxiliary shaft and the pivot, and fluorescence detection subassembly is fixed on the drive belt.

Among this technical scheme, the drive shaft rotates the driving-disc that can drive the rigid coupling with it and rotates, the driving-disc can drive its eccentric position's drive post rotation at the pivoted in-process, when the drive post removes in the drive hole, the drive post can drive the carousel and the pivot rotates certain angle at the pivoted in-process, and then make the drive belt remove certain distance between pivot and auxiliary shaft, can drive the fluorescence detection subassembly of fixing on the drive belt and slide one section distance along the casing, make the fluorescence detection subassembly remove the top of the reagent card that next awaits measuring, and convenient for operation.

Preferably, as an improvement, set up to the arc limit between two adjacent drive holes, coaxial rigid coupling has spacing dish on the driving-disc, and the arc limit can be laminated with the outer wall of spacing dish, is provided with curved holding tank on the spacing dish.

Among this technical scheme, after the drive post passes through the drive hole and drives the carousel and rotate certain angle, remove the back of taking one's place at fluorescence detection subassembly, the drive post shifts out the drive hole, and the circular arc limit can laminate with the outer wall of spacing dish this moment, can realize injecing of carousel position, when fluorescence detection subassembly detects, avoids the carousel to rotate and influences the effect that detects the development.

Preferably, as an improvement, the fluorescence detection assembly comprises a detection seat slidably connected to the housing, the detection seat is provided with a fluorescence detection head, and the detection seat is further fixed with a cover case capable of covering the reagent card seat.

Among this technical scheme, detect the seat and be used for supporting fluorescence detection head, through setting up the housing on detecting the seat for fluorescence detection head is when detecting reagent card, and the housing can cover and establish on reagent card, forms relatively independent darker detection space, avoids the fluorescence on external light source or the adjacent reagent card to cause the influence to the testing result, guarantees to develop clearly.

Preferably, as an improvement, the driving mechanism comprises a driving motor rotatably connected to the base, the base is further rotatably connected to a wheel shaft, a driving belt is connected between the wheel shaft and an output shaft of the driving motor, and a fastening member is connected between the driving belt and the reagent clamping seat located at the edge.

In the technical scheme, the driving motor rotates to drive the driving belt to move between the output shaft of the driving motor and the wheel shaft, and the reagent clamping seat is fixed on the driving belt through the fastening piece, so that the reagent clamping seat can be driven to move towards one end of the fluorescence detection head in the moving process of the driving belt; after the detection is finished, the driving motor rotates reversely, and the driving belt can drive the reagent card holder to move towards one end far away from the fluorescence detection head, so that batch card discharging of the reagent cards can be realized, and the card discharging operation efficiency is improved.

Preferably, as an improvement, two driving mechanisms are provided, and the two driving mechanisms are respectively arranged at two sides of the plurality of reagent cartridges.

In the technical scheme, the two driving mechanisms are respectively arranged on the two sides of the reagent clamping seat, so that the two driving mechanisms can cooperatively drive the reagent clamping seat to move, and the moving stability of the reagent clamping seat and the moving synchronism among the plurality of reagent clamping seats can be ensured.

Drawings

Fig. 1 is a top view of a housing according to a first embodiment of the present invention;

fig. 2 is a top view of the detecting mechanism according to the first embodiment of the present invention;

fig. 3 is a front longitudinal sectional view of a first embodiment of the present invention;

fig. 4 is a bottom view of the driving assembly according to the first embodiment of the present invention.

Detailed Description

The following is further detailed by way of specific embodiments:

reference numerals in the drawings of the specification include: the device comprises a shell 1, a base 2, a sliding groove 3, a reagent clamping seat 4, a driving motor 5, a wheel shaft 6, a driving belt 7, a fastening plate 8, a servo motor 9, a driving shaft 10, a driving disc 11, a limiting disc 12, a driving column 13, a rotating shaft 14, a rotating disc 15, a driving hole 16, an auxiliary shaft 17, a driving belt 18, a detection seat 19, a driving cylinder 20 and a housing 21.

Example one

The embodiment is basically as shown in the attached figures 1 and 2: the multichannel sliding type fluorescence immunoassay device comprises a shell 1, a driving mechanism and a detection mechanism.

The shell 1 plays a role in integral accommodation, a row of sequentially arranged card inserting ports are arranged on the side wall of the shell 1, and the arrow direction in fig. 1 is the direction of inserting the reagent card. The welding of the inside bottom of casing 1 has base 2, is provided with a plurality of horizontal spouts 3 on base 2, and equal sliding connection has reagent cassette 4 in spout 3, and reagent cassette 4 can be just to setting up with the bayonet socket, and is in the same place through the bolt fastening between the reagent cassette 4.

The driving mechanism is used for driving the reagent card holder 4 to slide along the sliding groove 3, and the driving mechanism is provided with two driving mechanisms which are arranged oppositely and respectively positioned at two ends (two sides of the reagent card holder 4) of the shell 1. Actuating mechanism all includes the driving motor 5 of rotation connection at the 2 left ends of base, the right-hand member of base 2 rotates and is connected with shaft 6, shaft 6 is on a parallel with driving motor 5's output shaft, and be connected with parallel driving band 7 between shaft 6 and driving motor 5's the output shaft, there is mounting plate 8 through the bolt fastening on driving band 7, the bolt fastening is passed through on the reagent cassette 4 of one side to the one end of mounting plate 8, two driving bands 7 are connected with the reagent cassette 4 that is located both sides through mounting plate 8 respectively promptly.

The detection mechanism is used for carrying out fluorescence detection on the reagent card, is positioned in the shell 1 and is arranged at one end far away from the card inserting port. The detection mechanism comprises a fluorescence detection assembly and a driving assembly for driving the fluorescence detection assembly to intermittently slide along the top of the shell 1.

Combine shown in fig. 3, drive assembly is including rotating servo motor 9 of connection in casing 1 top, and servo motor 9's output shaft sets up vertically downwards, and servo motor 9's the coaxial rigid coupling of output shaft has drive shaft 10, and drive shaft 10 rotates the top of connecting at casing 1. Referring to fig. 4, a driving disc 11 is coaxially and fixedly connected to the driving shaft 10, a limiting disc 12 concentrically arranged with the driving disc 11 is integrally connected to the driving disc 11, and an arc-shaped accommodating groove is formed in the limiting disc 12. A vertical driving column 13 is welded on the eccentric position of the driving disc 11, and the driving column 13 is positioned on the opposite side of the accommodating groove. The top of casing 1 rotates and is connected with vertical pivot 14, and pivot 14 is located one side of drive shaft 10, and the coaxial rigid coupling in bottom of pivot 14 has carousel 15, is provided with the driving hole 16 that can hold drive column 13 of four bars on the carousel 15, and driving hole 16 is along the radial setting of carousel 15, and the setting of driving hole 16 circumference equipartition on carousel 15. An arc edge is integrally formed between the side walls of the two adjacent driving holes 16 and can be attached to the outer wall of the limiting disc 12. A vertical auxiliary shaft 17 is rotatably connected in the shell 1, a parallel winding transmission belt 18 is connected between the auxiliary shaft 17 and the rotating shaft 14, and the fluorescence detection assembly is fixed on the transmission belt 18.

The fluorescence detection assembly comprises a detection seat 19 connected with the shell 1 in a transverse sliding mode, a driving cylinder 20 is fixed on the detection seat 19 through a bolt, a piston rod of the driving cylinder 20 is arranged downwards, and a fluorescence detection head is fixed at the bottom end of the piston rod. The detection seat 19 is also fixed with a cover 21 which can cover the reagent card seat 4 through bolts, the cover 21 is vertically arranged, the process and the principle of detecting the fluorescence on the reagent card by the fluorescence detection head in the embodiment and the related circuit are all the prior art, and the embodiment does not improve the process and the principle.

The specific implementation process is as follows: after dropwise add the sample that awaits measuring on the reagent card, insert the reagent card that awaits measuring in corresponding reagent cassette 4 from the bayonet socket respectively, then driving motor 5 starts and corotation, makes driving belt 7 slide reagent cassette 4 along spout 3 from the right side left under driving motor 5's drive effect, and when reagent cassette 4 slided the below of fluorescence detection head, driving motor 5 was in the shutdown state.

In an initial state, the fluorescence detection head is opposite to the leftmost reagent card holder 4, the driving cylinder 20 is started, the piston rod moves downwards, the fluorescence detection head and the cover 21 move downwards, the cover 21 covers the reagent card holder 4, and a dark environment beneficial to fluorescence detection is formed. The fluorescence detection head emits exciting light and performs fluorescence detection on the sample on the reagent card. After the detection is finished, the driving cylinder 20 drives the housing 21 and the fluorescence detection head to move upwards through the piston rod. At this moment, servo motor 9 starts, servo motor 9 drives drive shaft 10 and rotates, and then drive the driving-disc 11 of coaxial rigid coupling with it rotates, driving-disc 11 drives spacing dish 12 and drive post 13 and rotates, when drive post 13 rotated to its hold in drive hole 16, drive post 13 continued to rotate can drive carousel 15 and rotate certain angle, and then drive the pivot 14 rotation with carousel 15 fixed connection, make the drive belt 18 of connection between pivot 14 and auxiliary shaft 17 remove one section distance (the same with the distance between two reagent cassette 4). Drive belt 18 can drive and detect seat 19 and slide right along casing 1, and then drive fluorescence detection head and housing 21 and move right, it is just right to make fluorescence detection head and the next reagent card that waits to detect, then, drive actuating cylinder 20 restart, make fluorescence detection head and housing 21 move down once more, fluorescence detection is carried out to next reagent card, at this moment, drive post 13 does not hold in drive hole 16, drive-disc 11 rotates and can not drive carousel 15 and rotate, and the circular arc limit on the carousel 15 laminates with spacing dish 12's outer wall mutually, be as shown in fig. 4 state, spacing dish 12 can play spacing effect to carousel 15, guarantee that the stability of testing process goes on. The sequential detection of a row of reagent cards can be realized by repeating the steps.

After the whole row of reagent cards are detected completely, the driving motor 5 rotates reversely, and the driving motor 5 can drive the reagent card seat 4 to slide rightwards through the driving belt 7 when rotating reversely, so that automatic batch card discharging of the detected reagent cards can be realized, and an operator takes out the reagent cards and then puts in the next batch of reagent cards to be detected, so that the operation is convenient, and the detection continuity is high.

The above description is only an example of the present invention, and the detailed technical solutions and/or characteristics known in the solutions are not described too much here. It should be noted that, for those skilled in the art, without departing from the technical solution of the present invention, several modifications and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.

Claims

1. Multichannel slidingtype fluorescence immunoassay device, including the casing, the casing internal fixation has base, its characterized in that: a plurality of card insertion ports are formed in the side portion of the shell, a plurality of reagent card seats which are sequentially arranged and fixed with each other are connected to the base in a sliding mode, and the reagent card seats and the card insertion ports are arranged correspondingly; a driving mechanism for driving the reagent clamping seat to slide is arranged in the shell, and a detection mechanism which can be just opposite to the reagent clamping seat is connected to the top of the shell in a sliding mode.

2. The multi-channel sliding-type fluorescence immunoassay device according to claim 1, wherein: the detection mechanism comprises a fluorescence detection assembly and a driving assembly, wherein the fluorescence detection assembly is connected to the shell in a sliding mode, and the driving assembly is used for driving the fluorescence detection assembly to slide intermittently.

3. The multi-channel sliding-type fluorescence immunoassay device according to claim 2, wherein: the driving assembly comprises a driving shaft which is rotatably connected to the top of the shell, the driving shaft is coaxially and fixedly connected with a driving disc, and a driving column is fixedly connected to the eccentric position of the driving disc; the top of casing still rotates and is connected with the pivot, and the coaxial rigid coupling of pivot has the carousel, is provided with a plurality of driving holes that can hold the drive post on the carousel, and the driving hole sets up along pivot circumference equipartition, and the casing internal rotation is connected with the auxiliary shaft, is connected with the drive belt between auxiliary shaft and the pivot, fluorescence detection subassembly is fixed on the drive belt.

4. The multi-channel sliding-type fluorescence immunoassay device according to claim 3, wherein: set up to the arc limit between two adjacent drive holes, coaxial rigid coupling has spacing dish on the driving-disc, and the arc limit can be laminated with the outer wall of spacing dish, is provided with curved holding tank on the spacing dish.

5. The multi-channel sliding-type fluorescence immunoassay device according to claim 4, wherein: the fluorescence detection assembly comprises a detection seat which is connected with the shell in a sliding manner, a fluorescence detection head is arranged on the detection seat, and a cover shell which can cover the reagent clamping seat is fixed on the detection seat.

6. The multi-channel sliding-type fluorescence immunoassay device according to claim 5, wherein: the driving mechanism comprises a driving motor which is rotatably connected to the base, the base is further rotatably connected with a wheel shaft, a driving belt is connected between the wheel shaft and an output shaft of the driving motor, and a fastening piece is connected between the driving belt and the reagent clamping seat positioned on the edge.

7. The multi-channel sliding-type fluorescence immunoassay device according to claim 6, wherein: the two driving mechanisms are arranged on two sides of the reagent clamping seats respectively.