CN207992061U - A kind of high-flux medicaments sifting imaging device - Google Patents
A kind of high-flux medicaments sifting imaging device Download PDFInfo
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- CN207992061U CN207992061U CN201820120222.0U CN201820120222U CN207992061U CN 207992061 U CN207992061 U CN 207992061U CN 201820120222 U CN201820120222 U CN 201820120222U CN 207992061 U CN207992061 U CN 207992061U
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- 239000003814 drug Substances 0.000 title claims abstract description 25
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- 238000007877 drug screening Methods 0.000 abstract description 10
- 238000004458 analytical method Methods 0.000 abstract description 5
- 239000000523 sample Substances 0.000 description 23
- 238000000034 method Methods 0.000 description 14
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- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The utility model discloses a kind of high-flux medicaments sifting imaging devices, including cell incubator, it is fixed with holder in the cell incubator, it is fixed with porous plate on the holder, array is equipped with multiple micropores on the porous plate, the light source emitter irradiated to porous plate covering is fixed in the cell incubator right over porous plate, it is located in the cell incubator below holder and is fixed with the camera acted on porous plate, condenser lens is housed on the camera, the condenser lens is equipped with the first colour filter, the high-flux medicaments sifting imaging device further includes computer, the computer respectively with light source emitter, camera connects；It may be implemented to be conducive to the high efficiency and accuracy of raising drug screening without the function of time difference high throughput Image Acquisition and analysis to the micropore of porous plate.
Description
Technical field
The utility model is related to drug screening technology field more particularly to a kind of high-flux medicaments sifting imaging devices.
Background technology
During new drug development, drug screening be extracted from large-scale synthesis or native compound effectively, have
The targetedly key of target molecule.Drug screening refers to that may be used as the various substances used, including chemically synthesizedization
Object, polypeptide, natural products etc. are closed, using screening technique appropriate and screening technique, its pharmacology that may be present is detected and lives
Property, it is the important tie for connecting drug from experimental study to clinical application for the method that developing new drug provides experimental basis, it can be with
Efficiency of research and development is improved, shortens the period, reduces cost, reduces risk, new drug development is enable to continue to carry out.New development in recent years
Drug screening technology includes mainly High Throughput Screening Assay, high content screening, Applications of surface plasmon resonance and miniflow
Control chip drug screening technology.
Wherein, High Throughput Screening Assay is based on optical detection mostly and the molecular level or cellular level of foundation
Analyzing detecting method, including light absorption detection, fluoroscopic examination, chemiluminescence detection etc..Since High Throughput Screening Assay is being innovated
Have the characteristics that in the discovery procedure of primer it is quick, efficient, micro, its appearance decades between in, i.e., answered extensively
For in all parts of the world new drug research mechanism, the original new drug R＆D process of large-scale medical company.
Determination method based on fluorescent staining technique is usually used in the research of medicament high flux screening.Fluorophor is divided into
The fluorophor of inherent fluorophor and exogenous combination can not be examined when the fluorogen of macromolecular to be measured itself is not strong enough
When survey, in combination with some fluorescent dyes to reinforce its spectral signature.Fluorescence signal is not only very sensitive, is easy to detection, suitable for micro-
Quantization, and harmful pollution will not be caused to environment and human body.High-flux medicaments sifting process includes mainly sample preparation, model
Foundation, automatically screening and Correlation method for data processing etc., and it is mainly fluorescent technique to establish the detection technique that model uses at this stage.
Porous plate is widely used in drug screening, and present multi well plate system is gradually commercialized, day screening capacity can reach 100,000
More than secondary.
But lacks the method that porous plate is integrally imaged can hole-specifically can only scan porous plate, cause at present
The imaging existence time in each hole is poor, to carry out high flux screening to the sequential validity of a variety of drugs.
Utility model content
The purpose of this utility model is to provide a kind of high-flux medicaments sifting imaging device, the advantage is that, may be implemented
To the micropore of porous plate without the function of time difference high throughput Image Acquisition and analysis, be conducive to the high efficiency and standard that improve drug screening
True property.
The above-mentioned technical purpose of the utility model technical scheme is that:A kind of high-throughput drug sieve
Imaging device, including cell incubator are selected, holder is fixed in the cell incubator, is fixed on the holder porous
Plate, array is equipped with multiple micropores on the porous plate, is located in the cell incubator right over porous plate and is fixed with pair
The light source emitter of porous plate covering irradiation, interior be fixed with below holder of the cell incubator make porous plate
Camera, condenser lens is equipped on the camera, and the condenser lens is equipped with the first colour filter, the high-throughput drug sieve
It further includes computer to select imaging device, and the computer is connect with light source emitter, camera respectively.
By using above-mentioned technical proposal, different amounts of sample solution, light source hair are stored in each micropore of porous plate
Injection device is irradiated excitation sample to sample and sends out fluorescence, and camera, condenser lens and the first colour filter constitute optical imagery
Image is sent in computer by system, and fluorescence signal image is handled and analyzed by computer, parses each sample
The quantity of phosphor dot or the intensity of fluorescence in this, to obtain detection data, these detection datas reflect reagent sample
Function and effect, to realize that high-flux medicaments sifting, light source emitter irradiate the cover type of porous plate, be conducive to porous
Sample solvent in plate is carried out without time difference high throughput Image Acquisition, and the quick processing point to sample is then realized by computer
Analysis, effectively improves the high efficiency and accuracy of drug screening.
The utility model is further arranged to：The light source emitter includes that multiple arrays are fixed at cell culture
LED light in case right over porous plate, and the condenser that is arranged between LED light and porous plate, the LED light with it is micro-
Hole equivalent setting, and each LED light is located at the surface of each micropore on porous plate.
By using above-mentioned technical proposal, the light source Vertical Launch of LED light is in each micropore, to the sample in each micropore
The progress of this solvent is irradiated simultaneously without the time difference, and in the process, condenser may be implemented to polymerize light source, the light sent out so as to LED
Line forms the stronger light beam of directionality, is irradiated to sample solvent.
The utility model is further arranged to：The light source emitter further includes being arranged between LED light and porous plate
The second colour filter.
By using above-mentioned technical proposal, under the action of the second colour filter, it can limit and penetrate the second colour filter light
Wave-length coverage.
The utility model is further arranged to：The quantity of the micropore is 24 holes or 96 holes or 384 holes.
By using above-mentioned technical proposal, the replacement of a variety of porous plates can be carried out as needed.
The utility model is further arranged to：Second colour filter be arranged in LED light by detachable attachment mechanism and
Between porous plate.
By using above-mentioned technical proposal, can be continued at any time more by the second colour filter of detachable attachment mechanism pair at this time
It changes, to obtain the exciting light of different colours, to meet the needs of different sample detections.
In conclusion the utility model has the advantages that：
1, this imaging device may be implemented to be imaged the sample in porous plate in each micropore without the time difference, improve drug sieve
The high efficiency and accuracy of choosing；
2, light source emitter can ensure that the light source being radiated on sample solvent has higher intensity, to ensure sample
This smooth imaging；
3, this imaging device can replace the first colour filter and the second colour filter at any time according to actual state, to obtain
The exciting light of different colours, to meet the needs of different sample detections；
4, this multiwell plate format is various, can be replaced according to actual state.
Description of the drawings
Fig. 1 is the overall schematic of this high-flux medicaments sifting imaging device；
Fig. 2 is cell incubator inner light source emitter and the schematic diagram of detachable attachment mechanism in embodiment；
Fig. 3 is the partial enlarged view of A in Fig. 2.
In figure：1, cell incubator；11, holder；2, porous plate；21, micropore；3, light source emitter；31, LED light；
32, condenser；33, the second colour filter；4, camera；5, condenser lens；6, the first colour filter；7, computer；8, it is detachably connected
Mechanism；81, driving motor；82, terminal pad；83, step slot；84, gap slot.
Specific implementation mode
The utility model is described in further detail below in conjunction with attached drawing.
Embodiment：A kind of high-flux medicaments sifting imaging device, as illustrated in fig. 1 and 2, including cell incubator 1, cell training
It supports in case 1 and is fixed with holder 11, a porous plate 2 is fixed on holder 11, array is equipped with multiple micropores on porous plate 2
21, in cell incubator 1 the light source emitter to the covering irradiation of porous plate 2 is fixed with positioned at the surface of porous plate 2
3, light source emitter 3 includes that multiple arrays are fixed in cell incubator 1 positioned at the LED light of 2 surface of porous plate
31, quantity and 21 equivalent of micropore in porous plate 2 of LED light 31 are arranged, and each LED light 31 is arranged at each micropore 21
Surface, to ensure to the vertical irradiation of micropore 21；It is fixed with condenser 32 in the underface of LED light 31, so as to LED
The light sent out forms the stronger light beam of directionality, and vertical irradiation is carried out to the sample solvent in micropore 21.
As shown in Figs. 1-3, the second colour filter 33 is additionally provided between LED light 31 and condenser 32 at this time, is worn to limit
The wave-length coverage of saturating second colour filter, 33 light, the second colour filter 33 are realized by detachable attachment mechanism 8 in cell incubator 1
Interior placement, to change iridescent of the control to sample irradiation in micropore 21；Detachable attachment mechanism 8 includes by driving motor
81 controls are rotatably arranged on the terminal pad 82 in cell incubator 1 between LED light 31 and condenser 32, are set in terminal pad 82
There are the step slot 83 set for the second colour filter 33, the side of cell incubator 1 to be equipped with the gap slot produced for terminal pad 82
84, it is equipped with 84 closed lid of gap slot on cell incubator 1（It is not represented in figure）, to ensure cell incubator 1
Internal smooth experimental work.
As illustrated in fig. 1 and 2, at the same time, the lower section positioned at holder 11 in cell incubator 1 is fixed with to porous plate
The cameras 4 of 2 effects, condenser lens 5 is equipped on camera 4, and condenser lens 5 is equipped with the first colour filter 6, camera 4, condenser lens 5
And first colour filter 6 constitute to the imaging device of the sample solution in micropore 21, high-flux medicaments sifting imaging device also wraps
Computer 7 is included, computer 7 is connect with light source emitter 3, camera 4 respectively.
Specific implementation mode：Sample is prepared by micro-fluidic or conventional method, sample solution is added each in porous plate 2
In a micropore 21, changes the dosage of test agent or use a variety of different reagents and combinations thereof, realize in different micropores
Different reaction systems is set in 21；21 plate of micropore is placed on the holder 11 in cell incubator 1, and its position is made to be fixed on
On the position being aligned with light source emitter 3, it is ensured that entire porous plate 2 is hatched in the illumination zone of light source, and rotation connects
Disk 82 is connect, gap slot 84 is made it through and is produced out of cell incubator 1, the second colour filter 33 of selection is placed in step later
In slot 83, terminal pad 82 is gone in cell incubator 1 later.
Computer 7 controls LED light 31 and works later, and controls the power of light source so that light source passes through the second colour filter 33
Vertical irradiation is in each micropore 21 after obtaining required fluorescence and obtaining the spotlight effect of condenser 32, to excite sample to send out
Fluorescence, computer 7 control condenser lens 5 and carry out focusing work automatically, camera 4 to porous plate 2 carry out fluorescence signal image at
The acquisition of picture and image, the first colour filter 6 only allow the fluorescence of specific band to pass through for filtering out exciting light and stray light；
The first colour filter 6 herein can also be replaced, to adapt to the needs of different pattern detections.
Picture after imaging is presented in 7 system of computer, and carries out image processing and analysis by computer 7, is obtained
Information such as the distribution of fluorescence and intensity in each micropore 21, these message reflections function and effect of sample solvent, to realize
It is stored with different amounts of sample solution in each micropore 21 of high-flux medicaments sifting porous plate 2, is parsed glimmering in each sample
The quantity of luminous point or the intensity of fluorescence, to obtain detection data, these detection datas reflect the effect effect of reagent sample
Fruit, to realize high-flux medicaments sifting；Light source emitter 3 irradiates the cover type of porous plate 2, is conducive to porous plate 2
Interior sample solvent is carried out without time difference high throughput Image Acquisition, then realizes the fast processing and analysis to sample by computer 7,
Effectively improve the high efficiency and accuracy of drug screening.
In the present embodiment, the quantity of micropore 21 can be 24 holes or 96 holes or 384 holes in porous plate 2, to meet reality
It needs.
This specific embodiment is only the explanation to the utility model, is not limitations of the present invention, ability
Field technique personnel can as needed make the present embodiment the modification of not creative contribution after reading this specification, but
As long as all being protected by Patent Law in the right of the utility model.
Claims (5)
1. a kind of high-flux medicaments sifting imaging device, including cell incubator (1), it is characterised in that：The cell incubator
(1) it is fixed with holder (11) in, porous plate (2) is fixed on the holder (11), array is equipped on the porous plate (2)
Multiple micropores (21), interior be fixed with right over porous plate (2) of the cell incubator (1) cover porous plate (2)
The light source emitter (3) of irradiation, the cell incubator (1) is interior to be fixed with below holder (11) to porous plate
(2) camera (4) acted on, condenser lens (5) is equipped on the camera (4), and the condenser lens (5) is equipped with the first colour filter
(6), the high-flux medicaments sifting imaging device further includes computer (7), the computer (7) respectively with light source emitter
(3), camera (4) connects.
2. a kind of high-flux medicaments sifting imaging device according to claim 1, it is characterised in that：The light source transmitting dress
It sets (3) and includes that multiple arrays are fixed at LED light (31) in cell incubator (1) right over porous plate (2), and
The condenser (32) being arranged between LED light (31) and porous plate (2), the LED light (31) are arranged with micropore (21) equivalent, and
And each LED light (31) is located at the surface of each micropore (21) on porous plate (2).
3. a kind of high-flux medicaments sifting imaging device according to claim 2, it is characterised in that：The light source transmitting dress
It further includes the second colour filter (33) being arranged between LED light (31) and condenser (32) to set (3).
4. a kind of high-flux medicaments sifting imaging device according to claim 1, it is characterised in that：The micropore (21)
Quantity is 24 holes or 96 holes or 384 holes.
5. a kind of high-flux medicaments sifting imaging device according to claim 3, it is characterised in that：Second colour filter
(33) it is arranged between LED light (31) and condenser (32) by detachable attachment mechanism (8).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201820120222.0U CN207992061U (en) | 2018-01-24 | 2018-01-24 | A kind of high-flux medicaments sifting imaging device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201820120222.0U CN207992061U (en) | 2018-01-24 | 2018-01-24 | A kind of high-flux medicaments sifting imaging device |
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| CN207992061U true CN207992061U (en) | 2018-10-19 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111135088A (en) * | 2020-01-10 | 2020-05-12 | 广州中医药大学(广州中医药研究院) | High-flux medicine decocting and collecting device and method |
-
2018
- 2018-01-24 CN CN201820120222.0U patent/CN207992061U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111135088A (en) * | 2020-01-10 | 2020-05-12 | 广州中医药大学(广州中医药研究院) | High-flux medicine decocting and collecting device and method |
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Effective date of registration: 20240510 Address after: Room 726, Building A, Phase I, Zhongdan Ecological Life Science Industrial Park, No. 3-1 Xinjinhu Road, Nanjing, Jiangsu Province, 210000 Patentee after: Cambrian Zhiyuan (Nanjing) Biomedical Technology Co.,Ltd. Country or region after: China Address before: Building f7, Jiangsu Life Science and Technology Innovation Park, No. 9 Weidi Road, Qixia District, Nanjing City, Jiangsu Province, 210019 Patentee before: Zhao Xiangwei Country or region before: China |
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