CN212316137U

CN212316137U - Cell culture plate

Info

Publication number: CN212316137U
Application number: CN202020268204.4U
Authority: CN
Inventors: 沈健; 郭丰; 孙岩; 任佃云; 金鑫; 吴河水
Original assignee: Union Hospital Tongji Medical College Huazhong University of Science and Technology
Current assignee: Union Hospital Tongji Medical College Huazhong University of Science and Technology
Priority date: 2020-03-06
Filing date: 2020-03-06
Publication date: 2021-01-08
Anticipated expiration: 2030-03-06

Abstract

The utility model discloses a cell culture plate, including the culture plate body and the buffer sheet of rectangle, evenly distributed has a plurality of cultivation holes on the face of culture plate body, cultivates the pore wall downwardly extending in hole, and its bottom is sealed through the hole bottom, offers the slot of bar along the length direction of pore wall, and buffer piece accessible slot inserts cultivate the hole, the lower extreme edge of culture plate body still encloses the connecting frame that has closed the rectangle, and the one end of connecting frame is provided with the support, and the support closely cooperates with the connecting frame to can accomodate in the connecting frame, and when the support was opened, the face of culture plate body can form less than or equal to 45 the contained angle with the horizontal plane. An object of the utility model is to provide a can cushion liquid feeding pressure, prevent to cause the cell individual layer to drop at the liquid feeding in-process, reduce experimental error, avoid polluting the rifle head, and the tilting is placed, and the experimenter of being convenient for thoroughly draws the cell culture plate of cultivateing the cell in the hole.

Description

Cell culture plate

Technical Field

The utility model relates to a cell culture's technical field especially relates to a cell culture plate.

Background

Cell culture refers to a technique in which collected in vivo tissues are placed under sterile, nutrient conditions, appropriate temperature and PH to grow, propagate, and maintain their structures and functions. The morphological structure, the life activity and the change of living cells can be directly observed in cell culture, and the method plays an important role in research and application of cytology, genetics, virology and immunology. Cell culture plates are the most common cell culture devices in laboratories, where the number, quality and uniformity of a monolayer of cells is directly related to the results of subsequent experiments.

Chinese utility model patent CN205803500U discloses a cell culture plate, including culture plate body and apron, be equipped with a plurality of culture holes on the culture plate body, be equipped with at least one cell culture support in the culture hole, the cell culture support has the fibrous scaffold layer of a plurality of range upon range of settings, and fibrous scaffold layer has many cellosilks, many cellosilk parallel arrangement, it is adjacent two-layer the crisscross setting of cellosilk on fibrous scaffold layer. The cell culture plate can realize cell culture in a three-dimensional space through the cell culture bracket, and the cell culture surface area is increased under the condition of not increasing the space volume.

Chinese utility model patent CN202440518U discloses a cell culture plate, including culture plate and apron, be equipped with hasp i on two sides that the culture plate periphery is relative, be equipped with on two corresponding medial surfaces of apron and culture plate lock catch on II culture plates of hasp and be equipped with the handle on two sides relative in addition, this cell culture plate can reduce the pollution of cultivateing the cell through apron and hasp.

However, the above cell culture plate has the following disadvantages:

1. when carrying out the liquid feeding to above-mentioned cell culture board, because the adherent cell of growth in the cell culture board is not firm, consequently make the cell monolayer drop because of adding liquid very easily in the experimentation, lead to the experimental result error very big, and in order to avoid this kind of experimental error, the laboratory technician need support the rifle head and hold on the inner wall in cell culture hole, makes liquid slowly add, pollutes the rifle head very easily like this, consequently need frequently change the rifle head when carrying out the liquid feeding to the cell culture board, the operation is very loaded down with trivial details.

2. Above-mentioned cell culture board is dull and stereotyped structure, and when the laboratory technician need draw the cell, in order to guarantee to draw thoroughly, the one end that needs the laboratory technician to lift up the cell culture board in one hand makes the cell culture board slope, and the operation is drawed to the other hand, therefore the operation is extremely inconvenient.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a can cushion liquid feeding pressure, prevent to cause the cell individual layer to drop at the liquid feeding in-process, reduce experimental error, avoid polluting the rifle head, and the tilting is placed, and the laboratory technician of being convenient for thoroughly draws the cell culture plate of cultivateing the cell in the hole, aims at solving the technical problem explained in the background art.

In order to realize the above object, the utility model provides a cell culture plate, its key lies in, including the culture plate body and the buffer plate of rectangle, evenly distributed has a plurality of cultivation holes on the face of culture plate body, cultivate the pore wall downwardly extending in hole, and its bottom is sealed through the hole bottom, follows the slot of bar is seted up to the length direction of pore wall, the buffer plate accessible the slot inserts cultivate the hole, the lower extreme edge of culture plate body still encloses the link that closes the rectangle, the one end of link is provided with the support, the support with the link closely cooperates, and can accomodate in the link, and work as when the support was opened, the face of culture plate body can form less than or equal to 45's contained angle with the horizontal plane.

The support is a bent elastic pipe, two ends of the support are respectively connected with the connecting frame in a rotating mode, a limiting column is further arranged along the rotating direction of the support, and an inclined plane adaptive to the included angle is further arranged at the other end of the connecting frame.

Wherein, the connecting frame is surrounded with the water catch bowl rather than integrated into one piece all around.

Wherein, adjacent two are listed culture and be provided with the heating passageway between the hole, two joints of heating passageway pass respectively the water catch bowl outwards extends, and follows the length direction of heating passageway is provided with a plurality of warm braw holes.

Wherein, follow the length direction of water catch bowl is provided with two and slides stupefied relatively, cell culture plate still includes the apron, the both sides of apron seted up relatively with slide two spouts of stupefied complex.

The cover plate is provided with a plurality of through holes in an array mode, and the through holes are covered with filtering membranes.

The cover plate is also provided with a first identification area, a second identification area and a third identification area which are arranged in parallel.

Wherein the culture plate body, the hole wall, the hole bottom and the connecting frame are integrally formed.

Compared with the prior art, the beneficial effects of the utility model are that:

(1) set up on the pore wall with buffer plate complex slot, when the liquid feeding operation, the laboratory technician only need insert the buffer plate part through the slot and cultivate downtheholely, support the rifle head and hold and operate in the upper end of buffer plate, liquid can get into along with the buffer plate to fill to whole vestibule through the clearance of buffer plate and hole bottom. Because the upper end of the buffer sheet is not contacted with the hole wall, the gun head is not polluted, and the gun head is prevented from being frequently replaced while the cells are ensured to be uniform. In addition, cells are not attached to the buffer sheet, so that the cells are not damaged, and the accuracy and the reliability of a subsequent test result are ensured;

(2) set up the support that can accomodate, when the laboratory technician draws the cell, the laboratory technician can open the support, makes the face and the horizontal plane formation less than or equal to 45 contained angles of support culture plate body to make things convenient for the laboratory technician thoroughly to draw the cell in the culture hole, when putting into the incubator with the cell culture board and cultivateing, only need with the support accomodate in the link can. Meanwhile, when the experimenter carries out liquid adding operation, the bracket can be opened to enable the plate surface of the culture plate to be relatively inclined, and the buffer sheet forms a ramp inclined downwards, so that the drainage and buffering effects can be better achieved during liquid adding;

(3) the buffer sheet is completely inserted into the culture hole through the slot, so that the cell culture plate can also perform cell climbing experiments, and therefore, an experimenter does not need to additionally arrange a slide in the culture hole;

(4) cultivate the face that the hole wall extends the culture plate body for each culture hole is relatively independent, puts into the cell culture case with the cell culture board after, and heating installation production's heating installation can be through in the bottom entering connecting frame of connecting frame, and mutually independent pore wall makes the heated area increase of cultivateing the hole, thereby makes the ambient temperature in the culture hole maintain the within range that is favorable to cell growth.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a schematic perspective view of a cell culture plate according to the present invention in a first state;

FIG. 2 is a schematic bottom view of a cell culture plate according to the present invention in a first state;

FIG. 3 is a schematic perspective view of a cell culture plate according to the present invention in a second state;

FIG. 4 is a schematic bottom view of a cell culture plate according to the present invention in a third state;

1-culture plate body, 2-buffer sheet, 3-culture hole, 4-hole wall, 5-hole bottom, 6-slot, 7-connecting frame, 8-bracket, 9-limiting column, 10-inclined plane, 11-water collecting tank, 12-heating channel, 13-joint, 14-heating hole, 15-sliding edge, 16-cover plate, 17-sliding groove, 18-through hole, 19-first identification area, 20-second identification area and 21-third identification area.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.

In the description of the present invention, it is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are merely for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. In addition, in the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

Referring to fig. 1 to 4, the present invention provides a technical solution: the utility model provides a cell culture plate, its characterized in that, includes the culture plate body 1 and the buffering piece 2 of rectangle, evenly distributed has a plurality of cultivation holes 3 on the face of culture plate body 1, cultivate 4 downwardly extending of pore wall of hole 3, and its bottom is sealed through hole bottom 5, follows the slot 6 of bar is seted up to the length direction of pore wall 4, buffer piece 2 accessible slot 6 inserts cultivate hole 3, the lower extreme edge of culture plate body 1 still encloses the connecting frame 7 that closes the rectangle, the one end of connecting frame 7 is provided with support 8, support 8 with connecting frame 7 closely cooperates, and can accomodate in the connecting frame 7, and work as when support 8 is opened, the face of culture plate body 1 can form the contained angle that is less than or equal to 45 with the horizontal plane.

In this embodiment, compared with the prior art, the cell culture plate has the following beneficial effects:

set up slot 6 with buffer plate 2 complex on pore wall 4, when the liquid feeding operation, the laboratory technician only need insert culture hole 3 with buffer plate 2 part through slot 6 in, support the rifle head and hold and operate in the upper end of buffer plate 2, liquid can get into along with buffer plate 2 to fill to whole vestibule through the clearance of buffer plate 2 with hole bottom 5. Because the upper end of the buffer sheet 2 does not contact the hole wall 4, the gun head is not polluted, and the gun head is prevented from being frequently replaced while the cells are ensured to be uniform. In addition, cells are not attached to the buffer sheet 2, so that the cells are not damaged, and the accuracy and the reliability of a subsequent test result are ensured; set up the support 8 that can accomodate, when the laboratory technician draws the cell, the laboratory technician can open support 8, makes support 8 support the face of culture plate body 1 and horizontal plane formation less than or equal to 45 contained angles to make things convenient for the laboratory technician to thoroughly draw the cell in cultivateing hole 3, when putting into the incubator with the cell culture board and cultivateing, only need with support 8 accomodate in the splice frame 7 can. Meanwhile, when the experimenter carries out liquid adding operation, the bracket 8 can be opened to enable the surface of the culture plate to be relatively inclined, and the buffer sheet 2 forms a ramp inclined downwards, so that the drainage and buffer effects can be better achieved during liquid adding; the buffer sheet 2 is completely inserted into the culture hole 3 through the slot 6, so that the cell culture plate can also carry out cell climbing experiments, and therefore, an experimenter does not need to additionally arrange a slide in the culture hole 3; cultivate 3 pore walls 4 in hole and extend the face of cultivateing board body 1 for each cultivates 3 relatively independent in hole, after putting into the cell culture case with the cell culture board, the heating installation that heating device produced can get into in the connecting frame 7 through the bottom of connecting frame 7, and mutually independent pore wall 4 makes the heated area increase of cultivateing hole 3, thereby makes the ambient temperature who cultivates in the hole 3 maintain the within range that is favorable to cell growth.

Specifically, the support 8 is a bent elastic pipe, two ends of the support are respectively connected with the connecting frame 7 in a rotating manner, a limiting column 9 is further arranged in the rotating direction of the support 8, and an inclined plane 10 adapted to the included angle is further arranged at the other end of the connecting frame 7.

In this embodiment, because the support 8 has elasticity, therefore can realize with the close fit of linking frame 7, the support 8 also is difficult for deviating from when accomodating, when opening the support 8, the support 8 rotates certain position and can be blockked by spacing post 9 to make support 8 can be stable support culture plate body 1.

Specifically, the periphery of the connecting frame 7 is surrounded by a water collecting tank 11 which is integrally formed with the connecting frame.

In this embodiment, the humidity requirement of the cell culture can be ensured by adding a proper amount of distilled water to the water collecting tank 11, and thus the cell culture effect is better.

Furthermore, a heating channel 12 is arranged between two adjacent rows of the culture holes 3, two joints 13 of the heating channel 12 respectively penetrate through the water collecting tank 11 to extend outwards, and a plurality of heating holes 14 are arranged along the length direction of the heating channel 12.

In this embodiment, when the cell culture is performed, the outlet of the heating device of the cell culture box can be communicated with any interface of the heating channel 12, so that each culture hole 3 can be uniformly heated, and the temperature requirement of the cell culture can be further met.

Specifically, along the length direction of water catch bowl 11 is provided with two and slides stupefied 15 relatively, the cell culture plate still includes apron 16, the both sides of apron 16 relative seted up with slide stupefied 15 complex two spout 17.

Further, a plurality of through holes 18 are arrayed on the cover plate 16, and the through holes 18 are covered with a filter membrane.

In the present embodiment, the through-holes 18 covered with the filtration membrane can prevent contamination of cells while ensuring the oxygen concentration and the carbon dioxide concentration.

Specifically, the cover plate 16 is further provided with a first identification region 19, a second identification region 20 and a third identification region 21 which are arranged in parallel.

In this embodiment, the first label region 19 is used to label the type of cells being cultured, the second label region 20 is used to label the date of culturing the cells, and the third label region 21 is used to label the name of the experimenter.

Specifically, the culture plate body 1, the hole wall 4, the hole bottom 5 and the connecting frame 7 are integrally formed.

While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention.

Claims

1. The utility model provides a cell culture board, its characterized in that, includes the culture board body and the buffer plate of rectangle, evenly distributed has a plurality of cultivation holes on the face of culture board body, cultivate the pore wall downwardly extending in hole, and it is sealed that the bottom is passed through the hole bottom, follows the slot of bar is seted up to the length direction of pore wall, the buffer plate accessible the slot inserts cultivate the hole, the lower extreme edge of culture board body still encloses the connecting frame that has closed the rectangle, the one end of connecting frame is provided with the support, the support with the connecting frame closely cooperates, and can accomodate in the connecting frame, and work as when the support is opened, the face of culture board body can form less than or equal to 45's contained angle with the horizontal plane.

2. The cell culture plate according to claim 1, wherein the support is a bent elastic tube, two ends of the support are respectively rotatably connected with the connecting frame, a limiting column is further disposed along the rotation direction of the support, and an inclined plane adapted to the included angle is further disposed at the other end of the connecting frame.

3. A cell culture plate according to claim 1, wherein the connecting frame is surrounded on all sides by water collecting grooves formed integrally therewith.

4. The cell culture plate according to claim 3, wherein a heating channel is provided between two adjacent rows of the culture holes, two joints of the heating channel extend outward through the water collection grooves, and a plurality of heating holes are provided along a length direction of the heating channel.

5. The cell culture plate of claim 4, wherein two sliding ribs are oppositely arranged along the length direction of the water collecting groove, the cell culture plate further comprises a cover plate, and two sliding grooves matched with the sliding ribs are oppositely arranged on two sides of the cover plate.

6. A cell culture plate according to claim 5, wherein the cover plate has a plurality of through holes formed therein, and the through holes are covered with a filtration membrane.

7. A cell culture plate according to claim 6, wherein the cover plate further comprises a first marker region, a second marker region and a third marker region arranged in parallel.

8. A cell culture plate according to any of claims 1 to 7, wherein the plate body, the walls of the wells, the bottom of the wells and the connecting frame are integrally formed.