CN220064073U

CN220064073U - Box is hatched in dyeing of cell climbing piece

Info

Publication number: CN220064073U
Application number: CN202321327826.XU
Authority: CN
Inventors: 冯慧敏; 王晨琛
Original assignee: Shanghai Ruijikang Biopharmaceutical Co ltd
Current assignee: Shanghai Ruijikang Biopharmaceutical Co ltd
Priority date: 2023-05-29
Filing date: 2023-05-29
Publication date: 2023-11-21
Anticipated expiration: 2033-05-29

Abstract

The utility model provides a cell slide staining incubation box, which comprises: the tablet placing part is provided with a plurality of tablet placing grooves for placing cell climbing tablets, and the bottom of each tablet placing groove is provided with a liquid outlet; a knob and a baffle are correspondingly arranged near each film placing groove, the knob is connected with the baffle, and the baffle can close or open the liquid outlet by rotating the knob; a liquid storage part positioned below the liquid outlet hole; the box body comprises a box cover, and the sheet placing part and the liquid storage part are arranged in the box body in a drawable manner. The utility model has the advantages that each liquid outlet is independently controlled to be opened and closed by one knob, and an experimenter can simultaneously carry out the same or different experimental steps in a plurality of film placing grooves, thereby improving the experimental efficiency. In addition, the sheet placing part and the liquid storage part are arranged in the box body in a drawable manner, the assembly and the disassembly can be completed through pushing and pulling, and the sheet placing part is not required to be moved when the liquid storage part is moved, so that the cell climbing sheet displacement caused by the movement of the sheet placing part is avoided.

Description

Box is hatched in dyeing of cell climbing piece

Technical Field

The utility model relates to equipment for biological experiments, in particular to a cell slide staining incubation box.

Background

Cell staining is a technique of staining cells by chemical or antigen-antibody binding or the like, by which the growth state, morphology, molecular expression level, localization or the like of cells can be visually observed. Cell staining is typically performed by placing a cell slide in a cell culture dish, allowing cells to grow in the cell slide, and then imaging the cell slide after steps such as cell fixation, permeabilization, incubation with antibodies or staining solutions, washing, and the like. However, the cell climbing sheet made of glass material is easy to be adsorbed with the cell culture dish due to the existence of the culture solution, and the cell climbing sheet is easy to be crushed when the cell climbing sheet is taken out by forceps. When the cell staining is carried out, the cell climbing sheet is required to be moved into different culture dishes to carry out different experimental steps, so that the probability of breaking the cell climbing sheet is greatly increased, and the experimental time is prolonged. In addition, when the cell climbing sheet is incubated in the culture dish, the cell climbing sheet needs to be immersed by using a staining solution or an antibody, and the using amount of the staining solution or the antibody is large.

Disclosure of Invention

In view of the above drawbacks, an object of the present utility model is to provide a cell slide staining incubator that can perform multiple steps of cell staining.

The utility model provides a cell slide staining incubation box, which comprises: the tablet placing part is provided with a plurality of tablet placing grooves for placing cell climbing tablets, and the bottom of each tablet placing groove is provided with a liquid outlet; a knob and a baffle are correspondingly arranged near each film placing groove, the knob is connected with the baffle, and the baffle can close or open the liquid outlet hole by rotating the knob; the liquid storage part is positioned below the liquid outlet hole and is used for containing liquid flowing out of the liquid outlet hole; the box body comprises a box cover, and the sheet placing part and the liquid storage part are arranged in the box body in a drawable manner.

Preferably, the bottom of the box body is provided with water, four corners of the sheet placing part are respectively provided with a vent hole, and the vent holes are communicated with the bottom of the box body.

Preferably, the box body comprises a bottom plate, a front side plate, a left side plate, a rear side plate and a right side plate, wherein the front side plate, the left side plate, the rear side plate and the right side plate are respectively connected with the bottom plate, the rear side plate is connected with the box cover, the height of the front side plate is smaller than that of the left side plate and the right side plate, and a drawing opening is formed by surrounding the front side plate, the left side plate and the right side plate.

Preferably, the left side plate and the right side plate are respectively provided with a first T-shaped groove extending along the front-back direction, the sheet placing part is in sliding connection with the first T-shaped groove through a first T-shaped sliding block, and the sheet placing part can enter and exit the box body from the drawing port.

Preferably, a second T-shaped groove extending along the front-rear direction is arranged at the bottom of the sheet placing part, the liquid storage part is slidably connected with the second T-shaped groove through a second T-shaped sliding block, and the liquid storage part can enter and exit from the drawing port.

Preferably, a third T-shaped groove extending along the front-rear direction is respectively arranged on the left side plate and the right side plate, the third T-shaped groove is positioned below the first T-shaped groove, and the liquid storage part is in sliding connection with the third T-shaped groove through a third T-shaped sliding block.

Preferably, a handle is further arranged on the front side of the liquid storage part.

The utility model has the advantages of providing the cell climbing sheet dyeing incubation box capable of completing a plurality of steps of dyeing the cell climbing sheet in the sheet placing groove, effectively reducing the times of clamping the cell climbing sheet, reducing the probability of breaking the cell climbing sheet and reducing the using amount of dyeing liquid or antibody. Simultaneously, every liquid outlet hole is by a knob individual control switching, and the experimenter can put the piece groove in a plurality of and carry out the same or different experimental procedure simultaneously, promotes experimental efficiency. In addition, the sheet placing part and the liquid storage part are arranged in the box body in a drawable manner, the assembly and the disassembly can be completed through pushing and pulling, and the sheet placing part is not required to be moved when the liquid storage part is moved, so that the cell climbing sheet displacement caused by the movement of the sheet placing part is avoided.

Drawings

FIG. 1 is a schematic view of a box according to a first embodiment of the present utility model;

FIG. 2 is a schematic top and bottom structural view of a middle plate portion and a schematic structure of a liquid storage portion according to an embodiment of the present utility model;

FIG. 3 is an assembled front view of a cell slide staining incubator according to the first embodiment of the present utility model;

FIG. 4 is a schematic structural view of a second embodiment of the present utility model;

FIG. 5 is an assembled front view of a staining incubator for cell slide according to a second embodiment of the present utility model.

Description of element numbers:

1. box body

11. Box cover

12. Rear side plate

13. Left side plate

14. Front side plate

15. Right side plate

16. Bottom plate

2. Sheet placing part

21. Sheet placing groove

22. Liquid outlet hole

23. Vent hole

24. Second T-shaped groove

25. Board body

26. First T-shaped sliding block

27. Support leg

3. Knob

4. Baffle plate

5. Liquid storage part

51. Second T-shaped sliding block

52. Handle grip

53. Third T-shaped sliding block

54. Through hole

6. First T-shaped groove

7. Third T-shaped groove

Detailed Description

The following describes the embodiments of the present utility model in further detail with reference to the accompanying drawings. These embodiments are merely illustrative of the present utility model and are not intended to be limiting.

In the description of the present utility model, it should be noted that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, only for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the apparatus or elements to be referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

Furthermore, in the description of the present utility model, unless otherwise indicated, the meaning of "a plurality" is two or more.

Fig. 1 is a schematic view of a structure of a case, and in the following description, based on the drawing in fig. 1 as a reference of the direction, in fig. 1, the left side plate 13 is left, the right side plate 15 is right, the rear side plate 12 is rear, the front side plate 14 is front, and the direction perpendicular to the left-right, front-rear directions is up-down, wherein the bottom plate 16 is down.

Example 1

As shown in fig. 1-3, a first embodiment provides a cell slide staining incubation box, which comprises a slide placement part 2, a liquid storage part 5 and a box body 1. The sheet placing part 2 comprises a plate body 25 and supporting legs 27 respectively arranged at the left side and the right side of the bottom of the plate body 25, a plurality of sheet placing grooves 21 are formed in the plate body 25, openings are formed in the top and the bottom of the sheet placing grooves 21, specifically, the openings are in the shape of a round table with a big top and a small bottom, the diameter of each opening at the top is 12mm, and the openings are used for placing cell climbing sheets; the liquid outlet hole 22 at the bottom has a diameter of 3mm for discharging the liquid in the sheet-placing groove 21. A knob 3 and a baffle 4 are correspondingly arranged near each sheet placing groove 21, the knob 3 is connected with the baffle 4, and the baffle 4 can close or open the liquid outlet 22 by rotating the knob 3. I.e. the opening and closing of one liquid outlet hole 22 is independently controlled by one knob 3. The liquid storage part 5 is located below all the liquid outlet holes 22 and between two support legs 27, and is used for containing the liquid flowing out of the liquid outlet holes 22, and is in a rectangular groove shape in particular. The box body 1 comprises a box cover 11, and the sheet placing part 2 and the liquid storage part 5 are arranged in the box body 1 in a drawable manner. After the box cover 11 is opened, the assembly and the removal of the sheet placing part 2 and the liquid storage part 5 in the box body 1 can be completed through pushing and pulling; after the box cover 11 is covered, the sheet placing part 2 and the liquid storage part 5 are sealed in the box body 1, and the box body 1 is in a dark environment.

When the liquid outlet 22 is in a closed state, the experimenter can add the dyeing liquid, the antibody or the cleaning liquid into the tablet placing groove 21, open the liquid outlet 22 after the corresponding experimental steps are completed, discharge the liquid, and then close the liquid outlet 22 to carry out the next step. Therefore, the cell slide staining incubation box according to the first embodiment not only can utilize less staining solution or antibody to realize incubation of the cell slide, but also can directly clean the cell slide in the slide placement groove 21, so that the frequency of moving the cell slide is reduced, and the probability of breaking and losing cells of the cell slide is reduced. In addition, each liquid outlet hole 22 is independently controlled to be opened and closed by one knob 3, so that an experimenter can simultaneously perform the same or different experimental steps in a plurality of film placing grooves 21, and experimental efficiency is improved.

The box body 1 comprises a rectangular bottom plate 16, a front side plate 14, a left side plate 13, a rear side plate 12 and a right side plate 15 which are respectively connected with the bottom plate 16, the rear side plate 12 is connected with the box cover 11, the height of the front side plate 14 is smaller than that of the left side plate 13 and the right side plate 15, and a drawing opening is formed by surrounding the front side plate 14, the left side plate 13 and the right side plate 15. The left side plate 13 and the right side plate 15 are respectively provided with a first T-shaped groove 6 extending along the front-back direction, and the height of the first T-shaped groove 6 is larger than that of the front side plate 14; a first T-shaped slide 26 is provided on each of the two legs 27, and is slidably connected to the first T-shaped groove 6 via the first T-shaped slide 26. The first T-shaped groove 6 can limit the first T-shaped sliding block 26 up and down and left and right, only allows the sheet placing part 2 to move along the front and back direction and enter and exit the box body 1 from the drawing port, and thus the assembly between the sheet placing part 2 and the box body 1 is simpler and more convenient, and the installation is more stable and firm.

Similarly, two second T-shaped grooves 24 extending in the front-rear direction are provided at the bottom of the plate 25 between the two legs 27, and one second T-shaped slider 51 is provided at each of the left and right sides of the top of the liquid storage portion 5, and slidably connected to the second T-shaped grooves 24 via the second T-shaped slider 51. The second T-shaped groove 24 can limit the second T-shaped slider 51 vertically and horizontally, and only allows the second T-shaped slider 51 to move in the front-rear direction, so that the liquid storage portion 5 is firmly suspended below the sheet placement portion 2 and can be moved into and out of the box 1 through the drawing port. Preferably, the front side of the reservoir 5 is also provided with a handle 52, the handle 52 facilitating the removal and installation of the reservoir 5. In the experimental process, the liquid storage part 5 can be moved and the internal liquid can be cleaned under the condition that the sheet placing part 2 is not moved, so that the displacement of the cell climbing sheet caused by the movement of the sheet placing part 2 is avoided.

After the assembly is completed, the front side surfaces of the two support legs 27 and the front side surface of the liquid storage part 5 can jointly block foreign objects from falling into the bottom of the box body 1 from the drawing port, so that the internal environment of the box body 1 is polluted.

Further, water is contained in the bottom of the case 1, that is, in the space defined by the bottom plate 16, the front side plate 14, the left side plate 13, the rear side plate 12 and the right side plate 15, four corners of the sheet placing portion 2 are respectively provided with a vent hole 23 penetrating up and down, and the vent holes 23 penetrate through the plate body 25 and the legs 27 to communicate with the bottom of the case 1. The setting can make the cell climbing piece keep moist in the experimental process like this, avoids the cell dehydration phenomenon that the humidity caused excessively low, ensures good dyeing and incubates the effect.

Example two

As shown in fig. 4 and 5, the second embodiment is different from the first embodiment in that: the left side plate 13 and the right side plate 15 of the box body 1 are also provided with a third T-shaped groove 7 which extends along the front-back direction and is positioned below the first T-shaped groove 6; the sheet placing part 2 only comprises a plate body 25, and first T-shaped sliding blocks 26 are arranged on two sides of the plate body 25 and are in sliding connection with the first T-shaped grooves 6; the left and right side walls of the liquid storage part 5 are respectively provided with a third T-shaped slide block 53 to replace the second T-shaped slide block, and the third T-shaped slide block 53 is in sliding connection with the third T-shaped groove 7. Meanwhile, each of four corners of the liquid storage part 5 is provided with a through hole 54 communicated with the vent hole 23. After the assembly is completed, the front side surface of the liquid storage part 5 can prevent foreign objects from falling into the bottom of the box body 1 from the drawing port.

The foregoing is merely a preferred embodiment of the present utility model, and it should be noted that modifications and substitutions can be made by those skilled in the art without departing from the technical principles of the present utility model, and these modifications and substitutions should also be considered as being within the scope of the present utility model.

Claims

1. A cell slide staining incubation cassette comprising:

the tablet placing part is provided with a plurality of tablet placing grooves for placing cell climbing tablets, and the bottom of each tablet placing groove is provided with a liquid outlet; a knob and a baffle are correspondingly arranged near each film placing groove, the knob is connected with the baffle, and the baffle can close or open the liquid outlet hole by rotating the knob;

the liquid storage part is positioned below the liquid outlet hole and is used for containing liquid flowing out of the liquid outlet hole;

the box body comprises a box cover, and the sheet placing part and the liquid storage part are arranged in the box body in a drawable manner.

2. The cell slide staining incubation box of claim 1, wherein the bottom of the box body is filled with water, and four corners of the slide placement part are respectively provided with a vent hole, and the vent holes are communicated with the bottom of the box body.

3. The cell slide staining incubation box of claim 1, wherein the box body comprises a bottom plate, a front side plate, a left side plate, a rear side plate and a right side plate which are respectively connected with the bottom plate, the rear side plate is connected with the box cover, the height of the front side plate is smaller than that of the left side plate and the right side plate, and a drawing opening is formed between the front side plate, the left side plate and the right side plate in a surrounding mode.

4. The cell slide staining incubation box according to claim 3, wherein the left side plate and the right side plate are respectively provided with a first T-shaped groove extending along the front-back direction, the slide-placing part is slidably connected with the first T-shaped groove through a first T-shaped slide block, and the slide-placing part can enter and exit the box body from the drawing port.

5. The cell slide staining incubation box of claim 4, wherein a second T-shaped groove extending along the front-back direction is arranged at the bottom of the slide placement part, the liquid storage part is slidably connected with the second T-shaped groove through a second T-shaped slide block, and the liquid storage part can enter and exit the box body from the drawing port.

6. The cell slide staining incubator of claim 4, wherein the left side plate and the right side plate are each provided with a third T-shaped groove extending in the front-rear direction, the third T-shaped groove is located below the first T-shaped groove, and the liquid storage part is slidably connected with the third T-shaped groove through a third T-shaped slider.

7. The cell slide staining incubator of claim 1 wherein the front side of the reservoir is further provided with a handle.