CN113214993A

CN113214993A - Constant temperature and humidity incubator

Info

Publication number: CN113214993A
Application number: CN202110654698.9A
Authority: CN
Inventors: 胡明雨
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-06-11
Filing date: 2021-06-11
Publication date: 2021-08-06

Abstract

The invention belongs to the technical field of incubators, in particular to a constant-temperature and constant-humidity incubator, and aims at solving the problem of slow digestion and dispersion of cells on a single plane in the prior art, the incubator comprises an incubator with a cuboid box structure, partition plates are clamped in the bottom and the middle of the incubator, an inlet is formed in the front of the incubator, a box door is hinged to the edge of the inlet, a glass frame with a rectangular frame-shaped structure is connected to the upper surface of each partition plate in a sliding mode, an inclined supporting plate is fixed to the inner wall of each glass frame close to the bottom end of each glass frame, a glass bottom plate is clamped in the middle of each inclined supporting plate, and inclined planes which are consistent with the inclined angle of the inclined supporting plate and are mutually attached to the inclined planes are formed in the four edges of the lower surface of the glass floor. According to the invention, when cells are internally divided, the habit of adherence is generated, the arrangement can increase the extending direction of the cells during division, so that the traditional in-plane division is changed into spatial division, and the spatial enlargement enables the cells to be dispersed, thereby being easier to improve the survival rate.

Description

Constant temperature and humidity incubator

Technical Field

The invention relates to the technical field of cell culture boxes, in particular to a constant-temperature constant-humidity culture box.

Background

The bacteria incubator is a device for culturing, preserving and testing bacteria, mold, microorganisms and the like, is widely applied to the technical fields of food, biology and the like, and has higher and higher requirements along with the current continuous deep research on bacteria, for example, in the process of culturing cells, temperature and humidity have great influence on the culture result, and if the control is not good, the efficiency of cell culture is reduced and even fails.

The function of current bacteria incubator is fairly simple, and most of them only have the constant temperature regulating function, although can realize the temperature of control bacteria culture in-process, but can't control other condition, and the digestion and the dispersed cell process of microorganism on single plane of current incubator are carried on comparatively slowly, and even digestion and dispersed cell can't reach anticipated effect, make the result of the microorganism bacterium of current incubator culture when experimental detection is inaccurate, consequently just need a novel constant temperature and humidity incubator that can improve cell digestion dispersion.

Disclosure of Invention

The invention provides a constant temperature and humidity incubator, aiming at overcoming the problem that cells are decomposed and dispersed slowly on a single plane in the prior art.

In order to achieve the purpose, the invention adopts the following technical scheme: a constant temperature and humidity incubator comprises an incubator with a cuboid box structure, wherein partition plates are clamped in the bottom and the middle of the incubator, an inlet is formed in the front of the incubator, a box door is hinged to the edge of the inlet, a glass frame with a rectangular frame-shaped structure is connected to the upper surface of each partition plate in a sliding mode, an inclined supporting plate is fixed to the inner wall of each glass frame close to the bottom end, a glass bottom plate is clamped in the middle of the inclined supporting plate, inclined planes which are consistent with the inclined angle of the inclined supporting plate and are mutually attached to the inclined planes are formed in the four edges of the lower surface of the glass floor, so that when the incubator is used, the glass bottom plate can be tightly attracted to the upper surface of the inclined supporting plate under the action of gravity, the infiltration of a culture medium on the glass bottom plate is avoided, and when the culture medium on the glass bottom plate needs to be replaced, the waste liquid of the culture medium can be discharged only by lifting the glass bottom plate upwards, it is very convenient, place the direction glass ring that is the concentric circles distribution in the middle of the upper surface of glass bottom plate, and the middle vertical gusset that has radially dispersed that inserts of direction glass ring to can be when using, have adherent habit when the cell divides inside, the orientation that extends when so setting up can increase cell division, make traditional in-plane division become the schizolysis in the space, the grow in space makes the easier improvement survival rate of cell dispersion.

In a preferred embodiment of the present invention, a temperature regulator is fixed to a side surface of the incubator, and an air outlet communicating with the temperature regulator is provided on a back surface of the incubator near a top end thereof.

As a preferable scheme in the invention, a humidifier is fixed at the top end of the incubator, an outlet of the humidifier is communicated with a conveying pipe, atomizing nozzles are arranged on the conveying pipe right above the two partition plates, a sensor is further arranged on the inner wall of the incubator and comprises a temperature and humidity sensor, a carbon dioxide concentration sensor and a photosensitive sensor, the sensor is connected with a processing module through a signal line, and the processing module is connected with each control unit and a display screen through a signal line, so that the specific internal situation can be further known through the sensor, the temperature rise of the temperature rise can be automatically humidified by the humidification, and related links can be manually adjusted through data displayed by the display screen, so that the internal culture environment can reach an expected value.

As the preferable scheme in the invention, the clamping ring is clamped on the outer wall of the top end of the guide glass ring, and the cross-shaped connecting clamping block is clamped on the top end of the junction of the guide glass ring of the outer ring and the vertical rib plate, so that all vertically distributed adherence targets on the upper surface of the glass bottom plate can be connected together when the device is used, the position fixing, taking and placing become more convenient, and the loss of cells is reduced when the culture medium on the glass bottom plate is replaced.

As a preferable scheme in the invention, the middle part of the upper surface of the clapboard is provided with a liquid accumulation groove, the middle part of the bottom of the liquid accumulation groove is provided with a leakage hole, and the bottom end of the leakage hole is inserted with a discharge pipe.

According to the preferable scheme, grooves are formed in the upper surface of the partition plate close to the two sides, groove-shaped slide rails with upward openings are clamped in the grooves, limiting slide grooves are formed in the groove bottoms of the groove-shaped slide rails far away from the inlet ends, inner slide rods are connected in the groove-shaped slide rails in a sliding mode, protruding blocks matched with the limiting slide grooves are fixed at the rear ends of the lower surfaces of the inner slide rods, and magnet clamping grooves are formed in the upper surface of the inner slide rods close to the front ends and the rear ends.

As a preferable scheme in the invention, magnets are fixed on the four corners of the lower surface of the glass frame, and the shape and the position of each magnet are clamped with the distance between two adjacent magnet clamping grooves, so that the glass frame can be tightly adsorbed on the surface of the inner sliding rod when in use, and can only be pulled out by half of the width when the glass frame needs to be taken out, and the glass frame is prevented from being turned over due to the reduction of the bearing surface.

As the preferable scheme in the invention, a handle is fixed on the outer wall of the glass frame close to the top end, and a silica gel sleeve is sleeved on the outer wall of the glass frame.

As a preferable scheme in the invention, operation windows are arranged on the right side of the incubator and above the two clapboards, vertical sliding strips are connected to the middles of the operation windows in a sliding mode, foldable heat insulation materials are fixed on the side surfaces of the sliding strips and the vertical edges of the operation windows, and a shifting block is fixed on one side of the sliding strips, which is far away from the interior of the incubator.

To sum up, the beneficial effect in this scheme is:

1. according to the constant-temperature and constant-humidity incubator, the guide glass ring and the vertical rib plate are arranged on the upper surface of the glass bottom plate, so that adherence habits can be generated when cells are internally split when the incubator is used, the extending direction of the cells during splitting can be increased, the traditional in-plane splitting is changed into spatial splitting, and the cells are dispersed more easily to improve the survival rate due to the fact that the space is enlarged;

2. according to the constant-temperature and constant-humidity incubator, by arranging the clamping ring and the cross connecting clamping block which are clamped above the guide glass ring, all vertically distributed adherence targets on the upper surface of the glass bottom plate can be connected together when the incubator is used, so that the position is more convenient to fix, take and place, and the loss of cells is reduced when a culture medium on the glass bottom plate is replaced;

3. according to the constant-temperature and constant-humidity incubator, the magnets arranged at the four corners of the lower surface of the glass frame are matched with the magnet clamping grooves on the inner sliding rods, so that the glass frame can be tightly adsorbed on the surface of the inner sliding rods when in use, and can only be pulled out by half of the width when the glass frame needs to be taken out, and the glass frame is prevented from turning over due to the reduction of the bearing surface;

4. this kind of constant temperature and humidity incubator through the operation window on incubator right side that sets up, can be in the cultivation in-process, when need carry out interim sample, only need open an operation window and can stretch into and take a sample fast, avoids opening the chamber door and leads to the internal environment to acutely change.

Drawings

FIG. 1 is a schematic sectional view of a constant temperature and humidity incubator according to the present invention;

FIG. 2 is a schematic view of an assembly structure of a partition plate of a constant temperature and humidity incubator according to the present invention;

FIG. 3 is a schematic perspective view of a glass clamping mechanism of a constant temperature and humidity incubator according to the present invention;

FIG. 4 is a schematic structural view of a glass bottom plate of a constant temperature and humidity incubator according to the present invention;

FIG. 5 is a schematic perspective view of a partition plate of a constant temperature and humidity incubator according to the present invention.

In the figure: 1 incubator, 101 chamber door, 102 entry, 2 direction glass ring, 201 vertical gusset, 3 conveyer pipes, 4 atomizer, 5 glass bottom plates, 501 inclined plane, 6 magnet, 7 glass frame, 8 silica gel cover, 9 temperature regulator, 10 humidifier, 11 air outlet, 12 operation window, 13 insulation material, 14 stirring block, 15 draw runner, 16 slope layer board, 17 interior slide bar, 1701 magnet draw-in groove, 1702 lug, 18 sensor, 19 flute profile slide rail, 1901 spacing spout, 20 liquid accumulation groove, 21 discharge pipe, 22 baffle, 2201 leak hole, 23 clamp ring, 24 cross connection clamp splice.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.

In this example

Referring to fig. 1-5, a constant temperature and humidity incubator comprises an incubator 1 in a cuboid box structure, wherein partitions 22 are clamped in the bottom and the middle of the incubator 1, an inlet 102 is formed in the front of the incubator 1, a chamber door 101 is hinged to the edge of the inlet 102, a display screen and a control device are arranged on the front of the chamber door 101 and mainly used for displaying the temperature and humidity, the carbon dioxide concentration and a plurality of adjusting knobs in the incubator, the bottom end of the inlet 102 is equal to the height of the lower surface of the bottom partition 22, a glass frame 7 in a rectangular frame structure is connected to the upper surface of the partition 22 in a sliding manner, an inclined supporting plate 16 is fixed on the inner wall of the glass frame 7 close to the bottom end, a glass bottom plate 5 is clamped in the middle of the inclined supporting plate 16, inclined planes 501 which are consistent with the inclined angle of the inclined supporting plate 16 and are mutually attached to the inclined planes are formed at four edges of the lower surface of the glass floor plate 5, thereby can be when using, can tightly attract with the upper surface of slope layer board 16 under the effect of gravity as glass bottom plate 5, avoid the culture medium on the glass bottom plate 5 to ooze down, when needs are changed to the culture medium on the glass bottom plate, only need lift glass bottom plate 5 slightly and can be with culture medium waste liquid discharge, it is very convenient, place the direction glass ring 2 that is concentric circles distribution in the middle of the upper surface of glass bottom plate 5, and the middle vertical gusset 201 that radially spreads that has inserted of direction glass ring 2 to can be when using, have adherent habit when the cell is inside the division, the direction that extends when so setting up can increase the cell division, make traditional in-plane division become the schizolysis in space, the grow in space makes the easy improvement survival rate of cell dispersion more.

In the invention, a temperature regulator 9 is fixed on the side surface of the incubator 1, and an air outlet 11 communicated with the temperature regulator 9 is arranged on the back surface of the incubator 1 close to the top end.

Wherein, the top of incubator 1 is fixed with humidifier 10, and humidifier 10's exit intercommunication has conveyer pipe 3, conveyer pipe 3 all is provided with atomizer 4 directly over two baffles 22, incubator 1's inner wall still is provided with sensor 18, sensor 8 includes temperature and humidity sensor, carbon dioxide concentration sensor and photosensitive sensor, the sensor is connected with processing module through the signal line, processing module passes through signal line and connects each the control unit and display screen, thereby can be through the further understanding of sensor 18 to inside particular case, this intensification of automatic humidification should intensification of this humidification, also can be through the relevant link of the manual regulation of data that the display screen shows, so that inside cultivation environment reaches the expectation.

Wherein, the top outer wall joint of direction glass ring 2 has grip ring 23, and the juncture top joint of the direction glass ring 2 of outer lane and vertical gusset 201 has cross connection clamp splice 24, can be in the same place the adherence target connection of all vertical distributions of glass bottom plate 5 upper surface when using for the fixed of position is got and is put and become more convenient, is favorable to changing the loss that reduces the cell on glass bottom plate 5 during the culture medium.

Wherein, the middle part of the upper surface of the partition plate 22 is provided with a liquid accumulation groove 20, the middle of the bottom of the liquid accumulation groove 20 is provided with a leakage hole 2201, and the bottom end of the leakage hole 2201 is inserted with a discharge pipe 21.

The upper surface of the partition plate 22 is provided with grooves close to two sides, groove-shaped slide rails 19 with upward openings are clamped in the grooves, the groove bottoms of the groove-shaped slide rails 19 are far away from the inlet ends and are provided with limiting slide grooves 1901, the groove-shaped slide rails 19 are connected with inner slide bars 17 in a sliding mode, the rear ends of the lower surfaces of the inner slide bars 17 are fixed with protruding blocks 1702 matched with the limiting slide grooves 1901, and the upper surface of the inner slide bars 17 is provided with magnet clamping grooves 1701 close to the front end and the rear end.

The magnets 6 are fixed on the lower surface of the glass frame 7 at the four corners, and the shape and the position of each magnet 6 are clamped with the distance between the two adjacent magnet clamping grooves 1701, so that the glass frame 7 can be tightly adsorbed on the surface of the inner slide bar 17 when in use, and the glass frame 7 can be pulled out by half of the width when needing to be taken out, and the glass frame 7 is prevented from turning over due to the reduction of the bearing surface.

Wherein, the outer wall of glass frame 7 is close to the top and is fixed with the handle, and the outer wall of glass frame 7 cup joints silica gel cover 8.

Wherein, operation window 12 has all been opened to the top that the right side of incubator 1 is close to two baffles 22, and the equal sliding connection in centre of operation window 12 has vertical draw runner 15, the side of draw runner 15 all is fixed with folding insulation material 13 with the vertical edge department of operation window 12, one side that incubator 1 inside was kept away from to draw runner 15 is fixed with stirring piece 14, thereby can be at the cultivation in-process, when need carrying out interim sample, only need open an operation window 12 can stretch into and take a sample fast, avoid opening chamber door 101 and lead to the violent change of internal environment.

The working principle is as follows: before use, the inclined plane 501 at the edge of the bottom end of the glass bottom plate 5 and the upper surface of the inclined supporting plate 19 are wiped clean, and then the glass bottom plate 5 can be tightly attracted with the upper surface of the inclined supporting plate 16 under the action of gravity, so that the culture medium on the glass bottom plate 5 is prevented from infiltrating downwards; then, clamping the radial guide glass ring 2 and the vertical rib plate 201 on the upper surface of the glass bottom plate 5 by using the clamping ring 23 and the cross connecting clamping block 24, finally pouring calf serum, namely a culture medium and prokaryotic cells, and adjusting the temperature and humidity to culture; when the culture medium on the glass bottom plate 5 needs to be replaced, the culture medium waste liquid can be discharged only by slightly lifting the glass bottom plate 5; meanwhile, when the cell has the habit of adhering to the wall when inside division, the direction glass ring 2 that so sets up, the direction that extends when can increase cell division for division becomes the schizolysis in the space in traditional plane, and the grow in space makes the cell dispersed improve the survival rate more easily.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims

1. The utility model provides a constant temperature and humidity incubator, is including incubator (1) that is cuboid box structure, inside and the middle equal joint in bottom of incubator (1) have baffle (22), and openly of incubator (1) open there is entry (102), and the edge of entry (102) articulates there is chamber door (101), its characterized in that, the upper surface sliding connection of baffle (22) has glass frame (7) that is rectangle frame shape structure, the inner wall of glass frame (7) is close to the bottom mounting has slope layer board (16), and the middle joint of slope layer board (16) has glass bottom plate (5), four edges of the lower surface of glass floor (5) all open inclined plane (501) unanimous and the laminating of inclined plane each other with slope layer board (16) angle of inclination, place direction glass ring (2) that are concentric circles and distribute in the middle of the upper surface of glass bottom plate (5), and vertical rib plates (201) which are scattered in a radial manner are inserted into the middle of the guide glass ring (2).

2. The incubator according to claim 1, wherein a temperature regulator (9) is fixed on the side of the incubator (1), and an air outlet (11) communicated with the temperature regulator (9) is arranged on the back of the incubator (1) near the top.

3. The incubator according to claim 2, wherein the humidifier (10) is fixed to the top end of the incubator (1), the outlet of the humidifier (10) is communicated with the delivery pipe (3), the delivery pipe (3) is provided with the atomizing nozzles (4) directly above the two partition plates (22), the inner wall of the incubator (1) is further provided with the sensor (18), the sensor (8) comprises a temperature and humidity sensor, a carbon dioxide concentration sensor and a photosensitive sensor, the sensor is connected with the processing module through a signal line, and the processing module is connected with the control units and the display screen through signal lines.

4. The incubator according to claim 1, wherein the outer wall of the top end of the guide glass ring (2) is clamped with a clamping ring (23), and the top end of the junction of the guide glass ring (2) and the vertical rib plate (201) of the outer ring is clamped with a cross connecting clamping block (24).

5. The incubator according to claim 1, wherein the middle of the upper surface of the partition (22) is provided with a liquid collecting tank (20), the middle of the bottom of the liquid collecting tank (20) is provided with a leakage hole (2201), and the bottom end of the leakage hole (2201) is inserted with a discharge pipe (21).

6. The incubator according to claim 5, wherein the upper surface of the partition (22) is provided with grooves near both sides, and the grooves are clamped with groove-shaped slide rails (19) with upward openings, and the groove bottom of the groove-shaped slide rails (19) is provided with a limit slide groove (1901) far from the inlet end, the groove-shaped slide rails (19) are connected with inner slide bars (17) in a sliding manner, the rear ends of the lower surfaces of the inner slide bars (17) are fixed with bumps (1702) matched with the limit slide grooves (1901), and the upper surface of the inner slide bars (17) is provided with magnet clamping grooves (1701) near both the front and rear ends.

7. The incubator according to claim 6, wherein the magnets (6) are fixed on the lower surface of the glass frame (7) at four corners, and the magnets (6) are in shape and position to be engaged with the distance between two adjacent magnet slots (1701).

8. The incubator according to claim 1, wherein a handle is fixed on the outer wall of the glass frame (7) near the top end, and a silica gel sleeve (8) is sleeved on the outer wall of the glass frame (7).

9. The incubator according to claim 1, characterized in that the right side of the incubator (1) is provided with an operation window (12) above the two partition boards (22), the middle of the operation window (12) is slidably connected with a vertical slide bar (15), the side of the slide bar (15) and the vertical edge of the operation window (12) are fixed with foldable heat insulation materials (13), and one side of the slide bar (15) far away from the inside of the incubator (1) is fixed with a shifting block (14).