CN215250844U - Culture apparatus of cosmetics detect reagent box - Google Patents
Culture apparatus of cosmetics detect reagent box Download PDFInfo
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- CN215250844U CN215250844U CN202120563781.0U CN202120563781U CN215250844U CN 215250844 U CN215250844 U CN 215250844U CN 202120563781 U CN202120563781 U CN 202120563781U CN 215250844 U CN215250844 U CN 215250844U
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Abstract
The utility model discloses a culture apparatus of cosmetics detect reagent box, including the upper cover, hatch the cell, cultivate the room, cultivate the support, cultivate and hold in the palm and locate in the cultivation room, cultivate the dish outer edge and cultivate the room lateral wall and be connected, be equipped with a plurality of cultivation holes on the cultivation dish, it is cylindrical to hatch the cell, hatch cell bottom diameter less than or equal to and cultivate the hole aperture, it takes in cultivateing downthehole to hatch the cell middle part, the upper cover lid is on cultivateing the room, cover and cultivate the support, hatch the cell, be filled with the culture medium in the cultivation room, the inlet and the liquid outlet of cultivateing the room both sides are connected through the exit of pipeline with the pump respectively, the cell bottom height is hatched to the liquid outlet height less than or equal to, it is equipped with the liquid feeding hole that is used for the liquid feeding to cultivate the room lateral wall, trade liquid. The utility model can form a gas-liquid interface without adding a bracket, thereby greatly reducing the risk of bacteria contamination; accelerating the speed of material exchange between cells and the outside, accelerating the prophase proliferation of seed cells and the formation of later-stage cell keratinization, and improving the quality of the obtained cosmetic detection kit.
Description
Technical Field
The utility model belongs to the technical field of medical instrument, in particular to cosmetic detection kit's culture apparatus.
Background
The detection of skin corrosiveness and irritation of cosmetics and compounds is an important part of the evaluation of safety of skin toxicity. Traditionally, rabbit skin irritation test is the basis for determining skin irritation. However, the irritation test not only causes pain to animals, but also has difficulty in ensuring the safety of the test results applied to human body because of the great difference in the structure and function of the skin between different species. With the increasing call of animal protection organization, the successive legislation of each country is promoted to protect the welfare of experimental animals, and 3R (reduction, optimization and replacement) movement in the field of animal experiments is widely promoted. Meanwhile, animal experimental alternatives directly influence economic development in international trade in the form of technical barriers. Therefore, a novel tissue engineering skin is produced as a tool for detecting the irritation of chemicals, and lays a foundation for detecting the irritation of industrial chemicals and wastes thereof, medicines, cosmetics, herbicides, insecticides, food additives, daily necessities (such as cleaning agents), phytotoxins and the like to the skin.
In the existing stratum corneum forming stage of the gas-liquid interface culture of the cosmetic detection kit, a bracket needs to be additionally arranged so as to form the gas-liquid interface, and the risk of bacterial contamination is increased in the process. In the prior art, in the culture process, the exchange of substances between cells and the outside is mainly realized by the molecular diffusion movement of a culture medium, the prophase multiplication of seed cells and the formation of later-stage cell keratinization are influenced, and the quality of the obtained cosmetic detection kit is influenced.
Disclosure of Invention
The utility model aims at overcoming the defects in the prior art and providing a culture device of a cosmetic detection kit, which can support the incubation chamber through the culture support, adjust the liquid level of the culture medium through the liquid feeding hole, form a gas-liquid interface without an additional support and greatly reduce the risk of bacteria contamination; the culture medium is made to flow by the pump, so that the speed of material exchange between the cells and the outside is accelerated, the prophase proliferation of the seed cells and the formation of the later-stage cell keratinization are accelerated, and the quality of the obtained cosmetic detection kit is improved.
In order to realize the purpose, the utility model discloses a technical scheme is:
the utility model provides a culture apparatus of cosmetics detect reagent box, which comprises an upper cover, hatch the cell, cultivate the room, cultivate and hold in the palm and locate in the cultivation room, cultivate the dish outer edge and cultivate the room lateral wall and be connected, be equipped with a plurality of cultivation holes on the cultivation dish, it is cylindrical to hatch the cell, hatch cell bottom diameter less than or equal to cultivation hole aperture, it takes in cultivation hole to hatch the cell middle part, the upper cover lid is on cultivateing the room, cover and cultivate the support, hatch the cell, it is filled with the culture medium in the cultivation room, the inlet and the liquid outlet of cultivation room both sides are connected through the exit of pipeline with the pump respectively, the cell bottom height is hatched to liquid outlet height less than or equal to, cultivation room lateral wall is equipped with and is used for the liquid feeding, the liquid feeding hole of liquid changing.
The upper cover is provided with a plurality of steam guide bulges, and each steam guide bulge corresponds to the position of the incubation chamber.
The steam guide protrusion is a smooth arc-shaped protrusion.
The incubation cell comprises a cylindrical shell and a semipermeable membrane, the bottom of the shell is fixedly installed on the semipermeable membrane, a plurality of vertical positioning strips are arranged on the side face of the incubation cell shell, and the outer size of each positioning strip and the outer size of each incubation cell are larger than or equal to the diameter of each culture hole.
The cultivation dish top is equipped with the positioning disk, is equipped with on the positioning disk with cultivate the locating hole that the hole corresponds position and quantity, the external dimensions that the aperture of locating hole more than or equal to location strip and incubation cell formed.
The positioning plate is connected with the outer edge of the culture tray or the upper part of the culture chamber.
The incubation chamber comprises a truncated cone-shaped shell and a semipermeable membrane, the bottom of the shell is fixedly installed with the semipermeable membrane, and the aperture of the upper part is larger than that of the lower part.
The incubation chamber has a gap with the underside of the culture chamber.
The utility model has the advantages that:
1) the incubation small chamber is supported by the culture support, the liquid level height of the culture medium is adjusted by the liquid feeding hole, a gas-liquid interface can be formed without adding a support, and the risk of bacterial contamination is greatly reduced; the culture medium is made to flow by the pump, so that the speed of material exchange between the cells and the outside is accelerated, the prophase proliferation of the seed cells and the formation of the later-stage cell keratinization are accelerated, and the quality of the obtained cosmetic detection kit is improved.
2) Steam guide protrusion can condense the steam that every incubation cell evaporated alone and fall to every incubation cell in, prevents that steam from mixing, because during steam mixes, when one incubation cell is contaminated other incubation cells also can be contaminated, and this point can be prevented in condensation alone, and guide protrusion can accelerate steam to condense the low-falling, maintains that the culture medium total amount is stable.
3) Because the incubation cell is cylindrical, the positioning strip is required to play the roles of clamping and fixing, and the incubation cell is prevented from falling into the culture hole.
4) The positioning disc can prevent the incubation chamber from shaking, and a stable cultivation environment is provided.
5) The gaps between the bottom of the incubation small chamber and the culture support and the lower side surface of the culture chamber are convenient for the flowing of a culture medium, the molecular movement rate is improved, the speed of the exchange of the cells and the external substances is accelerated, the early-stage proliferation and the later-stage keratinization of the seed cells are accelerated, and the quality of the obtained cosmetic detection kit is improved.
Drawings
FIG. 1 is a schematic perspective view of a culture apparatus of the cosmetic detection kit of the present invention.
FIG. 2 is a schematic top view of a culture apparatus of the cosmetic detection kit of the present invention.
FIG. 3 is a schematic sectional view of the culture apparatus A of the cosmetic detection kit of the present invention.
In the figure, 1, a pump; 2. an upper cover; 3. incubating the chamber; 4. a positioning bar; 5. positioning a plate; 6. culturing trays; 7. a culture chamber; 8. a liquid adding hole; 9. a steam guide projection;
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are merely for convenience of description of the present invention, and do not indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
Example 1
As shown in fig. 1, 2, 3, a culture apparatus of cosmetics detect reagent box, including upper cover 2, hatch cell 3, cultivate room 7, cultivate and hold in the palm 6 and locate in cultivating room 7, cultivate the outer edge of dish and be connected with cultivating room 7 lateral wall, be equipped with a plurality of cultivation holes on the dish, it is cylindrical to hatch cell 3, it is less than or equal to cultivates the hole aperture to incubate cell 3 bottom diameter, it is downthehole to hatch cell 3 middle part, upper cover 2 covers on cultivateing room 7, cover and cultivate and hold in the palm 6, incubate cell 3, be filled with the culture medium in the cultivation room 7, the inlet and the liquid outlet of cultivateing room 7 both sides are connected through the exit of pipeline with pump 1 respectively, the height of cell 3 bottom is incubated to the liquid outlet height less than or, be equipped with the liquid feeding hole 8 that is used for the liquid feeding, trade the liquid on cultivateing room 7 lateral wall or the dish.
The upper cover 2 is provided with a plurality of steam guide protrusions 9, and each steam guide protrusion 9 corresponds to the position of the incubation chamber 3.
The steam guide protrusion is a smooth arc-shaped protrusion.
Cultivate the dish top and be equipped with positioning disk 5, be equipped with on positioning disk 5 with cultivate the locating hole that the hole corresponds position and quantity, the external dimension that the aperture of locating hole more than or equal to location strip 4 and incubation cell 3 formed is equipped with the liquid feeding hole that corresponds with position on cultivating the dish on the positioning disk.
The positioning plate 5 is connected with the outer edge of the culture tray 6 or the upper part of the culture chamber 7.
In other embodiments of the present invention, the incubation chamber 3 comprises a truncated cone-shaped housing and a semi-permeable membrane, the bottom of the housing is fixed to the semi-permeable membrane, and the upper aperture is larger than the lower aperture.
The lower side surfaces of the incubation chamber 3 and the culture chamber 7 have a gap.
The incubation small chamber 3 is supported by the culture support 6, the liquid level height of the culture medium is adjusted by the liquid adding hole 8, a gas-liquid interface can be formed without adding a bracket, and the risk of bacterial contamination is greatly reduced; the pump 1 is used for flowing the culture medium, so that the speed of exchanging substances between the cells and the outside is accelerated, the prophase proliferation of the seed cells and the formation of the later-stage cell keratinization are accelerated, and the quality of the obtained cosmetic detection kit is improved.
The steam guide protrusions 9 are able to condense the evaporated steam of each incubation cell 3 down into each incubation cell 3 individually, preventing steam mixing, as condensation alone may prevent one incubation cell 3 from being contaminated while the other incubation cells 3 are also contaminated when the steam mixes.
Because the incubation cell 3 is cylindrical, the positioning strip 4 is required to play a role in clamping and fixing, and the incubation cell is prevented from falling into the culture hole.
The positioning plate 5 can prevent the incubation chamber 3 from shaking, providing a stable cultivation environment.
Gaps are formed between the bottom of the incubation small chamber 3 and the bottom of the culture support 6 and the lower side surface of the culture chamber 7, so that the flowing of a culture medium is facilitated, the molecular movement rate is improved, the speed of material exchange between cells and the outside is accelerated, the early-stage proliferation and later-stage cell keratinization of seed cells are accelerated, and the quality of the obtained cosmetic detection kit is improved.
The method comprises the following operation steps: inoculating seed cells into the incubation chamber 3, placing the incubation chamber 7, adding an under-liquid culture medium into the liquid adding hole 8 to enable the under-liquid culture medium to be level with the liquid level in the chamber, opening the pump 1 to enable the culture medium in the incubation chamber 7 to flow, being beneficial to exchanging nutrient substances inside and outside the chamber, placing the whole device into a carbon dioxide incubator to carry out under-liquid culture, and replacing the liquid on time. After the completion of the submerged culture, gas-liquid interfacial culture was performed. Gas-liquid culture medium is added from the liquid adding hole 8, the liquid level of the gas-liquid culture medium is flush with the bottom of the small chamber so as to carry out gas-liquid interface culture, meanwhile, the pump 1 is started to enable the culture medium in the device to flow, so that the nutrient substance exchange between the inside and the outside of the small chamber is facilitated, the whole device is placed into a carbon dioxide culture box, and the liquid is changed on time until the culture is finished.
The foregoing is merely exemplary and illustrative of the structure of the invention, and various modifications, additions and substitutions as described in the detailed description may be made by those skilled in the art without departing from the structure or exceeding the scope of the invention as defined in the claims.
Claims (8)
1. The utility model provides a culture apparatus of cosmetics detect reagent box, which comprises an upper cover, hatch the cell, cultivate the room, cultivate and hold in the palm and locate in the cultivation room, cultivate the dish outer edge and be connected with cultivation room lateral wall, be equipped with a plurality of cultivation holes on the cultivation dish, it is cylindrical to hatch the cell, hatch cell bottom diameter less than or equal to cultivation hole aperture, a serial communication port, it takes at the cultivation downthehole to hatch the cell middle part, the upper cover lid is on cultivateing the room, cover and cultivate the support, hatch the cell, it is filled with the culture medium in the cultivation room, the inlet and the liquid outlet of cultivation room both sides are connected with the exit of pump through the pipeline respectively, the cell bottom height is hatched to liquid outlet height less than or equal to, be equipped with the liquid feeding hole that is used for the liquid feeding on cultivation room lateral wall or the cultivation dish, trade liquid.
2. The culture device of a cosmetic detection kit according to claim 1, wherein the upper lid is provided with a plurality of steam guide projections, each steam guide projection corresponding to the position of the incubation chamber.
3. The culture apparatus of a cosmetic detection kit as set forth in claim 2, wherein the steam guide protrusion is a smooth arc-shaped protrusion.
4. The culture device of a cosmetic detection kit according to claim 1, wherein the incubation chamber comprises a cylindrical housing and a semi-permeable membrane, the bottom of the housing is fixedly mounted with the semi-permeable membrane, the side of the housing of the incubation chamber is provided with a plurality of vertical positioning strips, and the outer dimension formed by the positioning strips and the incubation chamber is larger than or equal to the diameter of the culture hole.
5. The culture apparatus of the cosmetic detection kit according to claim 4, wherein a positioning plate is provided above the culture plate, positioning holes are provided in the positioning plate in positions and in numbers corresponding to the culture holes, the diameter of the positioning holes is not less than the outer dimension formed by the positioning strips and the incubation chamber, and liquid feeding holes are provided in the positioning plate in positions corresponding to the culture plate.
6. The culture apparatus for a cosmetic detection kit according to claim 5, wherein the positioning plate is connected to an outer edge of the culture tray or an upper portion of the culture chamber.
7. The culture device of a cosmetic detection kit according to claim 1, wherein the incubation chamber comprises a truncated cone-shaped housing, a semi-permeable membrane, the bottom of the housing being fixedly attached to the semi-permeable membrane, and the upper aperture being larger than the lower aperture.
8. The culture device of claim 1, wherein the incubation chamber has a gap from the lower side of the culture chamber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120563781.0U CN215250844U (en) | 2021-03-18 | 2021-03-18 | Culture apparatus of cosmetics detect reagent box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120563781.0U CN215250844U (en) | 2021-03-18 | 2021-03-18 | Culture apparatus of cosmetics detect reagent box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215250844U true CN215250844U (en) | 2021-12-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120563781.0U Active CN215250844U (en) | 2021-03-18 | 2021-03-18 | Culture apparatus of cosmetics detect reagent box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215250844U (en) |
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2021
- 2021-03-18 CN CN202120563781.0U patent/CN215250844U/en active Active
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