JPH053038Y2 - - Google Patents

Description

[Detailed description of the invention] (Field of industrial application) The present invention relates to an observation plate used when observing cells, tissues, etc. with a microscope, and in particular, the invention relates to an observation plate used when observing cells, tissues, etc. with a microscope. This invention relates to a culture observation plate that can be used for culture observation.

(Conventional technology) It has been widely practiced to observe cells using a microscope, but when observing changes in cells over a long period of time, a large amount of cells are placed in a culture vessel, and the cells are exposed to nutrient solution or carbon dioxide gas. The cells were cultured, and the cells were taken out and observed under a microscope at regular intervals (for example, every 2 to 3 hours).

Once the observation was complete, the cells were transferred back to the original culture vessel and cell culture continued. The reason for this is that no cell can survive in an environment other than a culture vessel, and therefore observation can only be performed for a short period of time (within a few minutes to 1 to 2 hours).

(Problems to be solved by the invention) The conventional observation method described above had the following drawbacks. First, cells must be moved from the incubator to the observation plate each time they are observed, which is a time-consuming procedure, and there is a risk that the cells may come into contact with bacteria in the air during movement. Furthermore, cells removed from the culture vessel are not necessarily the same cells as those observed, and it is not always possible to observe the same cells under the same conditions.

In addition, changes such as division of cells being cultured in the incubator,
In other words, accurate observations were not possible because it was not possible to observe the subtle changes in cells over time as they changed from moment to moment.

(Means for solving the problem) The present invention was made in view of the above-mentioned situation, and its purpose is to enable accurate and detailed observation of the same cell with a simple configuration. A culture tank is constructed, and this culture tank has a structure including a supply pipe and a discharge pipe for supplying and discharging a culture solution such as cells, and a supply pipe and a discharge pipe for carbon dioxide gas, etc.

(Function) Cells are placed in a culture tank constructed on the top of the observation plate and filled with a culture solution, etc., and observed with an inverted microscope from the bottom of the observation plate. By constantly replenishing the culture medium and carbon dioxide gas, the culture tank prevents the activity of the cells in the culture tank from decreasing, and changes in the cells can be observed at any time.

(Example) Next, an example of the present invention will be described based on the drawings. FIG. 1 is a sectional front view, FIG. 2 is a plan view, and FIG. 3 is an explanatory diagram of the optical system.

A hole is made in one side of the shear dish 1, and the bottom of a cylindrical culture tank 3 is fitted therein. A bottom plate 2 with a thickness of 0.25 mm or less is attached to the bottom of the shear plate 1.

A hole is bored in the side of the culture tank 3, and a supply pipe 5 for supplying the culture solution is fitted into this hole.
The other end is connected to a pump (not shown), and
A connecting pipe 6 is similarly provided on the opposite side and is connected to a counting tank 4, which will be described later. Further, the lid 31 of the culture tank 3 is provided with a supply pipe 91 and a discharge pipe 92 for carbon dioxide gas.

A counting tank 4 is fixed to one side of the shear dish 1.
The end of the aforementioned connecting pipe 6 is attached to the side of this counting tank 4 to communicate with the culture tank 3.
A culture solution discharge pipe 7 is formed on the opposite side. In addition, a PH meter 15 is attached to the lid 41, and a PH meter 15 is attached to the lid 41.
Measure and control PH concentration, etc. This counting tank 4
does not necessarily need to be provided on the shear plate 1, and if the strength of the bottom plate 2 is sufficient, observation may be performed using only the bottom plate 2 without providing the shear plate 1.

When using the observation plate of this embodiment configured as described above, the culture solution 11 is placed in the culture tank 3, and the cells are placed in the culture tank 3.

Then, the entire observation plate may be set on an inverted microscope as shown in the optical system of FIG. 3 for observation. Even after the observation is completed, the cells 10, which are supplied with the culture solution 11 from the supply pipe 8 and carbon dioxide gas from the pipe 91, continue to move in the culture tank 3 and undergo changes such as division. Therefore, there is no need to transfer the cells 10 from the observation plate to the culture vessel as in the conventional case, and it is therefore possible to accurately observe the same cells at any time under the same conditions.

In addition, it is possible to follow and observe changes over time, and it is also possible to continuously observe the effects of drugs and other factors on proliferation and differentiation of cells or tissues.

The old culture solution in the culture tank 3 is sequentially sent to the counting tank 4 through the connecting pipe 6. here
A PH meter 15 measures acidity, etc. in the culture solution. Since the preferred PH concentration is PH7, if there is a change in this value, the amount of culture solution delivered through the supply pipe 8 is adjusted. The surplus culture solution in the counting tank 4 is discharged to the outside through a pipe 7.

(Effects of the invention) As described above, according to the invention, the same cells, etc. can be easily observed under the same conditions at all times while culturing cells, etc., so that cell biological phenomena can be observed accurately and quickly. This has the advantage that it is possible to

[Brief explanation of the drawing]

The drawings show one embodiment of the present invention; FIG. 1 is a sectional front view, FIG. 2 is a plan view, and FIG. 3 is an explanatory diagram of the optical system. 1... Shear plate, 2... Bottom plate, 3... Culture tank, 4... Counting tank, 10... Cell.

Claims (1)

[Scope of Claim for Utility Model Registration] (1) A culture tank for culturing cells, tissues, etc. is arranged on the top surface, and a culture solution for culturing cells, etc. is supplied and discharged to this culture tank. A reflux type culture observation plate having a supply pipe and a discharge pipe formed therein, as well as a supply pipe and a discharge pipe for supplying carbon dioxide gas, etc., to the culture tank. (2) The scope of the claim for utility model registration, which is characterized in that a counting tank is connected to the culture tank by a connecting pipe and is configured adjacent to the culture tank to measure the PH concentration, etc. of the culture solution sent from the culture tank. The reflux culture observation plate according to item 1.