CN211528816U - Glass slide and sample processing device - Google Patents
Glass slide and sample processing device Download PDFInfo
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- CN211528816U CN211528816U CN202020521246.4U CN202020521246U CN211528816U CN 211528816 U CN211528816 U CN 211528816U CN 202020521246 U CN202020521246 U CN 202020521246U CN 211528816 U CN211528816 U CN 211528816U
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Abstract
The utility model provides a glass slide and a sample processing device, wherein the sample area of the glass slide comprises one or more first positions and one or more second positions, and the surface of the first position is provided with a hydrophobic coating; the second positions are provided with hydrophilic surfaces, the edge of each second position is at least partially contacted with the first position, and the hydrophilic surfaces of the second positions are used as bottom surfaces and form concave structures together with the side surfaces of the hydrophobic coating around the hydrophilic surfaces; the thickness of the hydrophobic coating is 10-70 mu m. The utility model discloses a slide glass and sample processing apparatus can be used to the dyeing of cell natural sedimentation method, and it replaces the traditional method that cuts the cover glass then pastes and carry out the immunostaining on the slide glass for cell film-making dyeing integration can ensure simultaneously that the zero cross contamination between the sample just is favorable to forming the cell that the individual layer tiling distributes, prevents nonspecific staining, reduces the demand of consumptive materials such as culture solution.
Description
Technical Field
The utility model relates to a pathology equipment technical field, more specifically relate to a slide glass and sample processing apparatus.
Background
Cytology is the subject of studying the chemical composition, morphology, structure and function of cells at the optical microscope level. In order to clearly observe the target cells, visual verification is usually performed by staining. The cytological staining method can be used for diagnosing the antibody in serum, is an antibody detection technology with the highest international sensitivity at present, and obviously improves the detection rate of the antibody compared with the traditional Elisa detection method.
However, in the current cytological staining method, a cover glass is treated before staining cells, then the cover glass is placed into a tissue culture vessel, the cells grow on the cover glass, then the cover glass full of the cells needs to be cut, and the cover glass is attached to a glass slide after being cut into regular squares for staining. The cutting process of the cover glass is strict, the operation is complex, and the cell activity and the staining result can be influenced if the operation time is too long. Meanwhile, the cells of the slide obtained by the traditional method are crowded and irregular in shape, the adjacent cell membranes are mutually overlapped, and the immunofluorescence staining result is not easy to distinguish the positive cells and is more prone to false positive.
In order to solve the problem, CN202089992U discloses a device for removable cell imaging, which can realize the integration of cell slide and staining, the cavity can be removed freely under the action of external force, and the cells on the bottom plate of the culture device can be fixed and stained after being removed, so as to facilitate the research of high-resolution microscopic imaging on the cells. However, such devices are relatively complex, and are relatively difficult to remove freely due to the use of adhesives, and also have the potential for cross-contamination during chamber removal, and it is difficult to control the formation of a monolayer of cells on the slide.
SUMMERY OF THE UTILITY MODEL
Problem to be solved by utility model
In order to solve the above-mentioned problem that prior art exists, the utility model provides a slide glass can ensure that zero cross contamination between the sample and be favorable to forming the cell that the individual layer tiling distributes. The utility model discloses still provide a sample processing apparatus simultaneously, simple structure is high-efficient, can realize cell film-making dyeing integration, need not to use the adhesive can freely remove, can effectively prevent simultaneously that the sample from spilling over and nonspecific staining, still can reduce the demand of consumptive materials such as culture solution in addition.
Means for solving the problems
The utility model provides a glass slide, the glass slide includes sample district and auxiliary area, wherein, the sample district includes one or more first positions and one or more second positions, the first position surface has hydrophobic coating; the second location has a hydrophilic surface,
the edge of each second position is at least partially contacted with the first position, and the hydrophilic surface of the second position and the side surface of the hydrophobic coating around the hydrophilic surface form a concave structure together as a bottom surface;
the thickness of the hydrophobic coating is 10-70 mu m.
In at least one embodiment, the hydrophobic coating has a thickness of 30 to 55 μm.
In at least one embodiment, the sample area consists of only one first location and a plurality of second locations.
In at least one embodiment, the sample area comprises one first location and one or more second locations, and the shape of each of the second locations is formed by the first locations surrounding it.
In at least one embodiment, the shape of the second location is selected from a circle, a square, or a rectangle.
In at least one embodiment, the shape of the second location is selected from a square.
In at least one embodiment, each of the second locations has an area no greater than 49 square millimeters.
In at least one embodiment, each of the second locations has an area of 40 to 46 square millimeters.
In at least one embodiment, the sample area includes 2 to 8 second locations.
In at least one embodiment, the sample area comprises 8 evenly distributed second locations, which are squares; further, the 8 uniformly distributed second positions are divided into two rows and arranged in parallel in the sample area.
In at least one embodiment, the area ratio of the sample region and the auxiliary region of the slide glass is 1.8-2.5: 1
In at least one embodiment, the aspect ratio of the sample area of the slide is 1.5-2.5: 1; further, the aspect ratio of the sample area was 2: 1.
In at least one embodiment, the second position is a square with a side length of 5-7 mm.
In at least one embodiment, the interval between the adjacent second positions is 7-13 mm, and the interval is the distance between the centers of the two adjacent second positions.
In at least one embodiment, the hydrophobic coating is a fluorocarbon coating.
In at least one embodiment, the hydrophobic coating is a teflon coating.
The utility model also provides a sample processing apparatus, sample processing apparatus includes: the slide, the detachable chamber and the chamber outer cover;
the detachable chamber comprises a bottom plate and one or more open chambers which are arranged on the upper surface of the bottom plate and are communicated with the upper part and the lower part of the concave structure of the glass slide, wherein the open chambers can be embedded into the concave structure of the glass slide, and the side wall of each open chamber can be attached to the inner wall of the concave structure.
In at least one embodiment, the height of the open chamber is 0.8-1.5 cm.
In at least one embodiment, the chamber cover is configured to cover the removable chamber.
In at least one embodiment, the open chamber is square in cross-section.
In at least one embodiment, the sample processing device comprises one of the aforementioned slides, a removable chamber, and a chamber cover.
In at least one embodiment, the sample processing device is used for natural sedimentation staining of cells.
Effect of the utility model
The utility model discloses a slide glass and sample processing apparatus can be used for the cell to subside the method dyeing naturally, and it has replaced the traditional method that cuts the cover glass then pastes and carry out the immunostaining on the slide glass, can directly change over to the cell with the transfection and carry out fixed immunostaining on the slide glass, and easy operation saves time and operating procedure, reaches the purpose of simplifying the flow for operations such as cell film-making, dyeing are convenient and fast more, thereby the simplicity of operation, technical reliability and application stability have been improved greatly.
And simultaneously the utility model discloses a slide glass and sample processing apparatus design is more simple and convenient high-efficient, need not to use the adhesive can freely remove, scribbles through the slide glass that adopts through hydrophilic treatment and increase the hydrophobic messenger and still can avoid cross contamination effectively even remove the cavity, in addition the utility model discloses be favorable to forming the cell that the individual layer tiling distributes, prevent nonspecific staining, reduce the demand of consumptive material such as culture solution. Utilize the utility model discloses a slide glass and sample processing apparatus are circular for the suitable class of interval through the cell that the natural sedimentation method obtained, and the background is clean, the cell form is complete clear, the cell is the individual layer and distributes, and positive cell performance is more obvious, also avoids appearing false positive result to improve diagnostic staff's work efficiency and the accuracy of diagnosis result.
Drawings
Fig. 1 is a schematic front view of a slide according to an embodiment of the present invention.
Fig. 2 is a schematic side view of a slide according to an embodiment of the present invention.
Fig. 3 is a schematic front view of a slide according to another embodiment of the present invention.
Fig. 4 is a schematic front view of a slide according to another embodiment of the present invention.
Fig. 5 is a schematic structural view of a sample processing device according to an embodiment of the present invention.
Fig. 6 is a schematic perspective exploded view of a sample processing device according to an embodiment of the present invention.
Figure 7 is a schematic view of a slide and removable chamber combination according to one embodiment of the present invention.
Fig. 8 is a schematic structural diagram of a sample processing device according to an embodiment of the present invention, which is used with a wet cartridge during a dyeing process.
Description of reference numerals:
100 a sample processing device;
1, glass slide; 10a sample area; 11 an auxiliary area; 10a first position; 10b a second position; 12 a hydrophobic coating; 13 concave structure
2 a removable chamber; 20 opening the chamber; 21 a base plate;
3 a chamber outer cover;
4, matching a wet box; 40 a base for a wet box; 41 to fit the lid of the wet box.
Detailed Description
Exemplary embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood that the detailed description is only intended to teach one skilled in the art how to practice the invention, and is not intended to exhaust all possible ways of practicing the invention, nor is it intended to limit the scope of the invention. The figures are not necessarily to scale and certain features of the invention may be exaggerated in scale or in somewhat schematic form in the interest of clarity and conciseness. In this specification, relative terms such as "lengthwise," "widthwise," "up," "down," "top" and "bottom," "front," "back," and derivatives thereof should be construed to refer to the orientation as then described or as shown in the drawing under discussion. These relative terms are for convenience of description and are not generally intended to require a particular orientation.
As used herein, the term "hydrophilic surface" means that the surface will form a contact angle of 60 ° or less with a pure water droplet resting on the surface. The term "hydrophobic coating" generally refers to a coating that imparts a contact angle of greater than or equal to 90 degrees when added to a smooth substrate.
The structure of a slide glass according to an embodiment of the present invention will be described below mainly with reference to fig. 1 to 2. Unless otherwise specified, the present invention will explain the positional relationship of the components in the vertical direction shown in fig. 1.
The slide glass 1 according to the present embodiment includes a sample region 10 and an auxiliary region 11. The sample area 10 is used for placing samples, further, the sample area 10 is used for cell culture, fixing and staining, and the auxiliary area 11 is used for holding and labeling. The size of the slide glass is not particularly limited, but in the present embodiment, the length of the slide glass 1 is 75cm, the width is 25cm, the length of the sample region 10 is 50cm, and the length of the auxiliary region 11 is 25 cm.
The main material of the glass slide 1 is selected from soda-lime glass or Permanox plastic, and the surface of the glass slide is subjected to hydrophilic treatment so as to be beneficial to cell adhesion. Conventional hydrophilic treatment methods such as hydrogen peroxide, concentrated sulfuric acid treatment or chromic acid soaking, plasma treatment and the like can be adopted.
The sample area 10 comprises 1 first location 10a and 8 second locations 10b, as can be seen in fig. 1 and 2, the first location 10a having a hydrophobic coating 12 on its surface (i.e. the diagonal line in fig. 1), and the second location 10b having a hydrophilic surface; the edges of each second site 10b are in contact with the first site 10a and their hydrophilic surfaces as a bottom surface and the sides of the hydrophobic coating 12 around them together form the recessed structures 13, in which the cells are cultured, fixed and stained, the hydrophilic bottom surfaces of the recessed structures 13 enhance the adhesion of the cells, and the hydrophobic coating printed between the recessed structures 13 reduces the possibility of cross-contamination between samples.
The hydrophobic coating may comprise one or more hydrophobic functional groups selected from alkyl, vinyl, phenyl, and fluoroalkyl groups, for example, the hydrophobic functional group may comprise, but is not limited to, an alkylsilane, a vinylsilane, a phenylsilane, or a fluoroalkylsilane. In some specific embodiments of the present invention, the hydrophobic coating is a fluorocarbon coating, and the fluorocarbon coating is mainly made of a film-forming material made of a fluororesin (such as PTFE, PVDF, PEVE, PVF) having chemical bonds of fluorine/carbon, so that the fluorocarbon coating has a very low surface energy, excellent hydrophobicity (maximum water absorption is less than 5%) and good oil repellency and antifouling property, and thus is suitable for the use of the present invention. In one embodiment, the hydrophobic coating is a teflon coating. In the utility model discloses in, the thickness of hydrophobic coating is 10 ~ 70 mu m. Further preferably, the thickness of the hydrophobic coating is 30-55 μm. In the embodiment shown in fig. 1, the thickness of the hydrophobic coating is 45 μm. Increasing the thickness of the coating further reduces the possibility of cross-contamination between samples, while increasing the capacity of the recess 13, allowing more cell culture medium to be added and reducing non-specific staining of peripheral cells, but not too large in view of cost and ease of obtaining a monolayer distribution of cells.
In fig. 1, the sample area 10 comprises 8 uniformly distributed second locations 10b, which are squares with a side length of 6.5mm, arranged in parallel in two rows in the sample area. In other embodiments of the present invention, the second position can be set to 4, 5, 8, 10 according to the actual detection requirement, and the shape of the second position can be selected from circular or rectangular besides square. In contrast, the second position is square, which together with the side of the surrounding hydrophobic coating forms a square hole, which saves space and also makes the area of the cell staining hole larger. In order to obtain a monolayer of cells by using the natural sedimentation method, in at least one embodiment, the area of each second position is not more than 49 square millimeters, further the area of each second position is 40 to 46 square millimeters, and in order to reduce cross contamination between samples, the interval between adjacent second positions 10b is 7 to 13mm, and the interval is the distance between the centers of two adjacent second positions 10 b. In the embodiment shown in fig. 1, the distance between the centers of the second positions 10b adjacent in the length direction is 11.5mm, the distance between the centers of the second positions 10b adjacent in the width direction is 8.5mm, the minimum distance between the edge of the second position 10b and the upper and lower edges of the sample area 10 is 4.0mm, and the minimum distance between the edge of the second position 10b and the left and right edges of the sample area 10 is 4.5 mm. The second position adopts the mode of arranging that the advantage lies in saving space, prevents liquid cross contamination in the square hole when the area is the biggest utilization.
To obtain the slide of the invention, one or more second positions 10b can be reserved on the slide with a hydrophilic surface, after which the deposition of the hydrophobic coating to form is carried out. The hydrophobic coating may be deposited onto the slide surface by any suitable method, including but not limited to vapor deposition, spraying, or printing. The sample area 10 may be deposited with a certain thickness of hydrophobic coating on all but the second locations 10b, or may be deposited locally (in the manner shown in fig. 3 and 4, the diagonal lines are hydrophobic coatings), as long as the edge of each second location 10b is at least partially in contact with the first location 10a, and the hydrophilic surface thereof serves as the bottom surface and forms a concave structure together with the side surface of the hydrophobic coating around the hydrophilic surface.
Next, the structure of a sample processing device according to an embodiment of the present invention will be described mainly with reference to fig. 5 and 6. The sample processing device 100 according to the present embodiment includes: slide 1, removable chamber 2, and chamber cover 3. The slide 1 may be the slide shown in fig. 1 and 2.
The detachable chamber 2 comprises a bottom plate 21 and 8 vertically-through open chambers 20 arranged on the upper surface of the bottom plate and corresponding to the concave structures 13 of the glass slide 1, wherein the height of each open chamber 20 is 1cm, each open chamber 20 can be embedded into the concave structure 13 of the glass slide 1, the side wall of each open chamber 20 can be attached to the inner wall of the concave structure 13, and the attachment means that the open chambers 20 are bonded to the inner wall of the concave structure 13, which is in contact with the side wall of the concave structure 13, with a bonding force of a certain degree, so that liquid cannot seep out during bonding, and the bonding force of a certain degree, which cannot affect the inner wall of the concave structure 13 during peeling, can be easily peeled, is utilized. The open chambers 20 are mainly used for dripping and culturing samples, and 8 open chambers are arranged to simultaneously carry out a plurality of parallel tests, so that the flux and the detection speed are improved. The material of the detachable chamber 2 may be a transparent polymer, such as transparent polystyrene. The detachable cavity 2 is combined with the glass slide 1 without sealing materials, and the sealing is realized by the embedding of the open cavity 20 and the glass slide concave structure 13, so that the sample in the concave structure 13 can be effectively prevented from overflowing to cause cross contamination, meanwhile, the detachable cavity 2 can be freely and easily removed without any tool under the action of external force, and the sealant contamination possibly caused during the removal is reduced.
When the cell culture device is used, the outer cover 3 of the chamber is used for covering the upper part of the detachable chamber 2, a closed culture environment is provided for cells, and nonspecific coloring caused by poor cell growth state due to volatilization of a culture medium is effectively prevented. In this embodiment, the chamber cover 3 has dimensions of 41.7cm in length, 17.5cm in width and 0.5cm in height. In other embodiments of the present invention, the chamber cover 3 may further have a handle.
The utility model discloses a slide glass and sample processing apparatus can be used for cell culture and microscopic observation, cell natural sedimentation method dyeing, long-time living cell imaging observation etc. mainly is fit for adherent cell's cultivation, can realize cell preparation dyeing integration, improves diagnostic efficiency. When the cell culture medium is used specifically, the detachable chamber 2 is firstly embedded into the concave structure of the glass slide 1 (the combination effect is shown in figure 7), then a small amount of culture medium containing transfected cells is added, and the cell culture medium can be placed into an incubator for continuous culture after the outer cover 3 of the chamber is covered. After the culture is finished, the chamber outer cover 3 and the detachable chamber 2 are taken down to be fixed and dyed. In the dyeing process, the glass slides 1 are placed in a matching wet box 4 shown in figure 8 to be protected from light, the matching wet box 4 comprises a base 40 and a cover 41, the size of the base 40 is 30cm long, 10cm wide and 2.5cm high, the size of the cover 41 is 30cm long, 10cm wide and 2.0cm high, 10 glass slides can be placed in the matching wet box 4 side by side, and the distance between the glass slides is 2.8 cm. From which slides can be removed and coverslipped for direct observation under a high resolution microscope. Through the utility model discloses a device and method only need a small amount of cell and reagent just can obtain the circular cell of the type that the interval is suitable, and the background is clean, the cell form is complete clear, the cell is the individual layer and distributes, and positive cell performance is more obvious to improve diagnostic staff's work efficiency and diagnostic result's accuracy.
Of course, the present invention is not limited to the above embodiments, and those skilled in the art can make various modifications to the above embodiments of the present invention without departing from the scope of the present invention.
Claims (9)
1. A slide comprising a sample region and an auxiliary region,
the sample area comprises one or more first positions and one or more second positions, and the surface of each first position is provided with a hydrophobic coating; the second location has a hydrophilic surface,
the edge of each second position is at least partially contacted with the first position, and the hydrophilic surface of the second position and the side surface of the hydrophobic coating around the hydrophilic surface form a concave structure together as a bottom surface;
the thickness of the hydrophobic coating is 10-70 mu m.
2. The slide of claim 1, wherein the sample area includes a first location and one or more second locations, and wherein each of the second locations is shaped to be surrounded by the first location.
3. The slide of claim 1 or 2, wherein the second location has a shape selected from the group consisting of circular, square, and rectangular.
4. The slide of claim 1 or 2, wherein each of the second locations has an area no greater than 49 square millimeters.
5. The slide of claim 1 or 2, wherein the sample area comprises 2-8 second locations.
6. The slide of claim 4, wherein the sample area includes 8 evenly distributed second locations, the second locations being squares.
7. The slide of claim 1 or 2, wherein the hydrophobic coating is a fluorocarbon coating.
8. A sample processing device, characterized in that,
the sample processing device includes: the slide, the removable chamber, and the chamber cover of any one of claims 1 to 7;
the detachable chamber comprises a bottom plate and one or more open chambers which are arranged on the upper surface of the bottom plate and are communicated with the upper part and the lower part of the concave structure of the glass slide, wherein the open chambers can be embedded into the concave structure of the glass slide, and the side wall of each open chamber can be attached to the inner wall of the concave structure.
9. The device of claim 8, wherein the height of the open chamber is 0.8-1.5 cm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202020521246.4U CN211528816U (en) | 2020-04-10 | 2020-04-10 | Glass slide and sample processing device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202020521246.4U CN211528816U (en) | 2020-04-10 | 2020-04-10 | Glass slide and sample processing device |
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| CN211528816U true CN211528816U (en) | 2020-09-18 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112444478A (en) * | 2019-09-02 | 2021-03-05 | 华中师范大学 | Sample pool for blood cell imaging counting and preparation method and application thereof |
| CN113073029A (en) * | 2021-03-17 | 2021-07-06 | 长春长光辰英生物科学仪器有限公司 | Infiltration modified cell sorting chip for laser induced transfer and sorting method |
-
2020
- 2020-04-10 CN CN202020521246.4U patent/CN211528816U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112444478A (en) * | 2019-09-02 | 2021-03-05 | 华中师范大学 | Sample pool for blood cell imaging counting and preparation method and application thereof |
| CN112444478B (en) * | 2019-09-02 | 2023-10-17 | 华中师范大学 | Sample cell for blood cell imaging counting and preparation method and application thereof |
| CN113073029A (en) * | 2021-03-17 | 2021-07-06 | 长春长光辰英生物科学仪器有限公司 | Infiltration modified cell sorting chip for laser induced transfer and sorting method |
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