CN211528016U - Glass slide convenient to liquid nitrogen is frozen - Google Patents
Glass slide convenient to liquid nitrogen is frozen Download PDFInfo
- Publication number
- CN211528016U CN211528016U CN201922278999.7U CN201922278999U CN211528016U CN 211528016 U CN211528016 U CN 211528016U CN 201922278999 U CN201922278999 U CN 201922278999U CN 211528016 U CN211528016 U CN 211528016U
- Authority
- CN
- China
- Prior art keywords
- liquid nitrogen
- slide
- dull polish
- glass
- line board
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 72
- 239000011521 glass Substances 0.000 title claims abstract description 49
- 239000007788 liquid Substances 0.000 title claims abstract description 36
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 36
- 230000008014 freezing Effects 0.000 claims abstract description 24
- 238000007710 freezing Methods 0.000 claims abstract description 24
- 239000002245 particle Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract description 5
- 239000008187 granular material Substances 0.000 abstract description 2
- 239000006059 cover glass Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 244000050510 Cunninghamia lanceolata Species 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 210000000349 chromosome Anatomy 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- IAKHMKGGTNLKSZ-INIZCTEOSA-N (S)-colchicine Chemical compound C1([C@@H](NC(C)=O)CC2)=CC(=O)C(OC)=CC=C1C1=C2C=C(OC)C(OC)=C1OC IAKHMKGGTNLKSZ-INIZCTEOSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 2
- 238000010009 beating Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229960001338 colchicine Drugs 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 230000000442 meristematic effect Effects 0.000 description 1
- 230000031864 metaphase Effects 0.000 description 1
- 230000011278 mitosis Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model discloses a slide glass convenient to liquid nitrogen is frozen, including transparent bottom plate, dull polish line board and hole, transparent bottom plate is located this slide glass's lower extreme, transparent bottom plate's left side is provided with dull polish line board, and dull polish line board is for erecting dull polish line board, the upper left corner of dull polish line board is provided with the hole, and this slide glass convenient to liquid nitrogen is the glass material, and the upper surface of dull polish line board is provided with dull polish granule. This slide glass convenient to liquid nitrogen is frozen is provided with transparent bottom plate, dull polish line board and hole, and when doing permanent section and putting into liquid nitrogen with the slide glass and freezing, the hole of available hook catching slide glass is convenient for take out, can not freeze the hand. Meanwhile, holes are reserved on the glass slide, so that the slide surface can be conveniently distinguished for observation.
Description
Technical Field
The utility model belongs to the technical field of the slide glass, specifically be a slide glass convenient to liquid nitrogen is frozen.
Background
Slides are common devices used to slice or carry biological specimens and place test materials during testing, and to place cell or tissue slices on slides during specimen preparation. At present, in experiments, when a tabletting method is used for preparing a permanent specimen for plant mitosis, tweezers are often used for clamping a glass slide, the glass slide is put into liquid nitrogen for freezing (about 30s-60s), and then the glass slide is clamped out by the tweezers, so that the method is inconvenient. Therefore, there is a need to develop a slide that facilitates liquid nitrogen freezing operations.
Disclosure of Invention
The above-mentioned problem to prior art exists, the utility model aims to solve the technical problem that a slide glass convenient to liquid nitrogen is frozen is provided to solve present slide glass at present, put into the liquid nitrogen and freeze reuse tweezers clamp and get out, inconvenient problem.
In order to solve the technical problem, the utility model discloses a technical scheme be:
the utility model provides a slide glass convenient to liquid nitrogen is frozen, includes transparent bottom plate, dull polish line board and hole, transparent bottom plate is located this slide glass's lower extreme, the left side of transparent bottom plate is provided with dull polish line board, and dull polish line board is for erecting dull polish line board, the upper left corner of dull polish line board is provided with the hole.
Furthermore, the glass slide convenient for liquid nitrogen freezing is made of glass.
Further, the slide glass convenient for liquid nitrogen freezing is 7.5cm in length, 1.2mm in height and 2.5cm in width.
Furthermore, the upper surface of the frosted pattern plate is provided with frosted particles.
Further, the length of the frosted pattern plate is 2cm, and the width of the frosted pattern plate is 2.5 cm.
Further, the diameter of the hole is 3 mm.
Further, the length of the transparent bottom plate is 5.5cm, and the width of the transparent bottom plate is 2.5 cm.
Has the advantages that: compared with the prior art, the method has the following advantages:
this slide glass convenient to liquid nitrogen is frozen is provided with transparent bottom plate, dull polish line board and hole, and when doing permanent section and putting into liquid nitrogen with the slide glass and freezing, available hook catches on the slide glass hole, is convenient for take out, can not freeze the hand. Meanwhile, holes are reserved on the glass slide, so that the slide surface can be conveniently distinguished and observed.
Drawings
FIG. 1 is a top view of the slide facilitating freezing by liquid nitrogen;
FIG. 2 is a front view of the slide facilitating freezing by liquid nitrogen;
FIG. 3 is a left side view of the slide facilitating freezing by liquid nitrogen.
Detailed Description
The present invention will be further described with reference to the accompanying drawings.
As shown in fig. 1, fig. 2 and fig. 3, the glass slide convenient to liquid nitrogen is frozen of this application, including transparent bottom plate 1, dull polish board 2 and hole 3, transparent bottom plate 1 is located the lower extreme of this glass slide, the left side of transparent bottom plate 1 is provided with dull polish board 2, transparent bottom plate 1 and dull polish board 2 design as an organic whole, stability is improved, do not influence the observation, and dull polish board 2 is for erecting the dull polish board, improve frictional force, the upper left corner of dull polish board 2 is provided with hole 3, when making permanent section and putting into liquid nitrogen with the glass slide and freezing, available hook catches on the glass slide hole, be convenient for take out, can not freeze the hand. Meanwhile, holes are reserved on the glass slide, so that the slide surface can be conveniently distinguished and observed.
The glass slide convenient for liquid nitrogen freezing is made of glass, glass is transparent, pollution-free, fashionable and strong, the modeling is rich and the application is wide, the cost is low, the molding size of the mold is accurate, and the glass slide convenient for liquid nitrogen freezing is made of glass and has the highest cost performance.
The slide convenient for liquid nitrogen freezing has a length of 7.5cm, a height of 1.2mm and a width of 2.5 cm.
The upper surface of dull polish line board 2 is provided with dull polish granule, improves frictional force, conveniently takes.
The length of the frosted grain plate 2 is 2cm, and the width is 2.5 cm.
The diameter of the hole 3 is 3 mm.
The transparent substrate 1 was 5.5cm long and 2.5cm wide.
The specific working steps of the glass slide convenient for freezing by liquid nitrogen are as follows:
material taking: firstly, soaking Chinese fir seeds for a plurality of hours, then transferring the Chinese fir seeds into a culture dish padded with wet filter paper, placing the Chinese fir seeds into a constant-temperature incubator at 25 ℃ for germination, and taking materials when the radicles grow to 1-2 cm.
Pretreatment: and (3) placing the taken root tips into a penicillin bottle filled with 0.1% colchicine aqueous solution, and soaking for 3-4 h.
Fixing: the pretreated root tips are washed with water and fixed for 6-24 hours by using an ethanol-glacial acetic acid (v: v ═ 3:1) fixing solution. The fixing material can be transferred into 70% alcohol, and stored in a refrigerator at 4 deg.C for use.
Dissociation: pouring out the fixing solution, rinsing with distilled water for 2-3 times, putting into preheated 1mol/L hydrochloric acid, dissociating in a water bath at 60 ℃ for 10-15 min, and rinsing with distilled water for 2-3 times.
Dyeing: and (3) placing the root tip on a glass slide, cutting off the top end of the glass slide to be 1-2 mm, and dripping the Kabaohuang dye liquor for dyeing for about 15 min.
Tabletting: covering a cover glass, and lightly beating the cover glass by using a forceps handle to spread meristematic cells into a thin layer; then, the excess dye solution was aspirated off with filter paper, and the cells and chromosomes were dispersed by beating with a rubber tip of a pencil.
Microscopic observation: carefully observing under a microscope, and searching metaphase with clear chromosome contour, moderate staining, dispersion and no overlap.
Sealing: and (3) putting the pressed chromosome specimen in liquid nitrogen for quick freezing for 30-60 s, then quickly opening the cover glass by using a blade, and simultaneously drying the cover glass and the glass slide in an oven at about 37 ℃. After the glass slide and the cover glass are transparent by dimethylbenzene, neutral gum is dripped to seal the slide, and then the permanent slide is prepared. The glass slide and the cover glass are respectively sealed by a new cover glass and a new glass slide.
The utility model provides a thought and implementation method of slide glass convenient to liquid nitrogen is frozen, the concrete application way is many, above only the utility model discloses a preferred embodiment should point out, to the ordinary skilled person in this technical field, not deviating from the utility model discloses under the prerequisite of principle, can also make a plurality of improvements, these improvements should also be regarded as the utility model discloses a protection scope.
Claims (7)
1. A glass slide convenient for freezing of liquid nitrogen is characterized in that: including transparent bottom plate (1), dull polish line board (2) and hole (3), transparent bottom plate (1) is located the lower extreme of this slide glass, the left side of transparent bottom plate (1) is provided with dull polish line board (2), and dull polish line board (2) are perpendicular dull polish line board, the upper left corner of dull polish line board (2) is provided with hole (3).
2. The slide facilitating freezing of liquid nitrogen of claim 1, wherein: the glass slide convenient for liquid nitrogen freezing is made of glass.
3. The slide facilitating freezing of liquid nitrogen of claim 2, wherein: the glass slide convenient for liquid nitrogen freezing has the length of 7.5cm, the height of 1.2mm and the width of 2.5 cm.
4. The slide facilitating freezing of liquid nitrogen of claim 1, wherein: the upper surface of the frosted pattern plate (2) is provided with frosted particles.
5. The slide facilitating freezing of liquid nitrogen of claim 4, wherein: the length of the frosted pattern plate (2) is 2cm, and the width of the frosted pattern plate is 2.5 cm.
6. The slide facilitating freezing of liquid nitrogen of claim 1, wherein: the diameter of the hole (3) is 3 mm.
7. The slide facilitating freezing of liquid nitrogen of claim 1, wherein: the length of the transparent bottom plate (1) is 5.5cm, and the width of the transparent bottom plate is 2.5 cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922278999.7U CN211528016U (en) | 2019-12-17 | 2019-12-17 | Glass slide convenient to liquid nitrogen is frozen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922278999.7U CN211528016U (en) | 2019-12-17 | 2019-12-17 | Glass slide convenient to liquid nitrogen is frozen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211528016U true CN211528016U (en) | 2020-09-18 |
Family
ID=72445894
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922278999.7U Expired - Fee Related CN211528016U (en) | 2019-12-17 | 2019-12-17 | Glass slide convenient to liquid nitrogen is frozen |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211528016U (en) |
-
2019
- 2019-12-17 CN CN201922278999.7U patent/CN211528016U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20201202 Address after: 605 Zhushan Road, Jiangning District, Nanjing City, Jiangsu Province 210000 Patentee after: Nanjing houbai Biotechnology Co.,Ltd. Address before: Longpan road Xuanwu District of Nanjing city of Jiangsu Province, No. 159 210037 Patentee before: NANJING FORESTRY University |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200918 |