CN216445378U - Primary myocardial cell slide culture system - Google Patents
Primary myocardial cell slide culture system Download PDFInfo
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- CN216445378U CN216445378U CN202122463043.1U CN202122463043U CN216445378U CN 216445378 U CN216445378 U CN 216445378U CN 202122463043 U CN202122463043 U CN 202122463043U CN 216445378 U CN216445378 U CN 216445378U
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- slide
- culture dish
- glass slide
- glass
- culture system
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- 230000002107 myocardial effect Effects 0.000 title abstract description 15
- 239000011521 glass Substances 0.000 claims abstract description 49
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 18
- 238000004113 cell culture Methods 0.000 claims description 13
- 210000004413 cardiac myocyte Anatomy 0.000 claims description 9
- 238000005192 partition Methods 0.000 claims description 8
- 239000011247 coating layer Substances 0.000 claims description 6
- 230000002940 repellent Effects 0.000 claims 3
- 239000005871 repellent Substances 0.000 claims 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 3
- 238000004043 dyeing Methods 0.000 abstract description 3
- 241001391944 Commicarpus scandens Species 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 22
- 230000009194 climbing Effects 0.000 description 5
- 239000006285 cell suspension Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000002055 immunohistochemical effect Effects 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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Abstract
The utility model provides a primary myocardial cell glass slide culture system which comprises a glass slide and a square culture dish for accommodating the glass slide, wherein the glass slide comprises a glass slide body, the head of the glass slide body is provided with a handheld part, the upper surface of the glass slide body is provided with a circular hydrophobic frame, a circular hole surrounded by the hydrophobic frame is a limiting hole, and an inclined plane is arranged in the middle of the top end of the handheld part of the glass slide. The primary myocardial cell slide culture system provided by the utility model overcomes the problems that the conventional cell slide is easy to break and lose, the cell distribution is uneven, the experiment content is single, the dyeing, observation and photographing of the slide by an automatic instrument are inconvenient, and the like.
Description
Technical Field
The utility model relates to the field of laboratory consumables, in particular to a glass slide for primary myocardial cell culture and a matched culture dish system thereof.
Background
After the primary myocardial cells are successfully separated and purified, the adherence time is long, the survival period is short, the requirement on the growth environment is high, and the proliferation and the passage cannot be realized.
Traditional cell climbing film is the cell suspension dropwise add after will digesting on circular thin climbing film, and this kind of traditional cell climbing film exists easy breakage, easily loses, be not convenient for batch dyeing, experimental content is single, be not convenient for observe the scheduling problem of shooing. In addition, the conventional myocardial cell slide method easily causes the myocardial cells to gather in the gaps between the slide and the culture holes, so that the number of the cells on the myocardial cell slide is small, and the myocardial cells are not easy to survive.
Traditional slide glass compares with traditional cell slide, though has firmly, is difficult for losing, has instruments such as the automatic staining instrument of being convenient for, automatic immunohistochemical appearance in batches to dye, advantage such as automatic observation is shot, but traditional slide glass can not fix in an within range containing a certain amount of primary cardiomyocyte suspension, makes the adherence at the assigned position, causes the cell to run off, and cell adherence is small in quantity, is difficult for surviving. The area of the hydrophobic coating of the conventional in-situ hybridization glass slide coated with the hydrophobic layer is too large, so that the periphery of the limiting hole is covered, the cell growth condition outside the limiting hole is not convenient to determine, and the cell quantity is not favorably controlled. And secondly, the bottom of the traditional glass slide is smooth, and when the traditional glass slide is immersed in a culture solution, the bottom of the traditional glass slide is easily attached to the bottom of a culture dish and is not easy to take out by using sterile tweezers.
At present, a culture dish special for glass slide climbing is not available, and the existing culture dish for culturing the glass slide climbing has the defects of waste of culture solution, improper shape, inconvenience in grouping treatment and the like. Therefore, a glass slide which is suitable for directly inoculating a certain amount of primary cardiomyocytes and is convenient to clamp is sought, and a matched culture dish which is suitable for group culture is a problem which needs to be solved urgently by workers studying primary cardiomyocytes.
Disclosure of Invention
The utility model aims to solve the defects in the prior art and provide a cell slide culture system which is simple in structure and suitable for the growth of primary myocardial cells.
The technical scheme adopted by the utility model is as follows: the utility model provides a primary myocardial cell slide glass culture system, includes slide glass and the square culture dish that holds the slide glass, the slide glass includes the slide glass body, the head of slide glass body is equipped with handheld portion, the upper surface of slide glass body is equipped with the hydrophobic frame of circle ring shape, the circular port that hydrophobic frame encloses is spacing hole, the handheld portion top intermediate position of slide glass is equipped with the inclined plane, the highest point on inclined plane is 1mm with square culture dish bottom distance.
Further, the square culture dish comprises a cell culture dish body and a culture dish cover, a partition plate is arranged at the bottom of the cell culture dish body, and the cell culture dish body is divided into cells matched with the glass slide in shape by the partition plate.
Furthermore, the hydrophobic frame is a hydrophobic coating layer, the thickness of the coating layer is 5-25 μm, the width of the hydrophobic frame is 3mm, the inner diameter of the limiting hole is 12mm, and the volume of the limiting hole is 500 μ L.
Further, the width of the inclined plane is 5 mm.
Furthermore, scales are arranged on the side wall of the partition plate.
The beneficial effects obtained by the utility model are as follows: the primary myocardial cell slide culture system provided by the utility model overcomes the problems that the conventional cell slide is easy to break and lose, the cell distribution is uneven, the experiment content is single, the dyeing, observation and photographing of the slide by an automatic instrument are inconvenient, and the like.
Drawings
FIG. 1 is a schematic front view of a slide according to the present invention;
FIG. 2 is a right side view of a slide of the present invention;
FIG. 3 is a bottom view of a slide according to the present invention;
FIG. 4 is a cross-sectional view of a slide of the present invention;
FIG. 5 is a schematic view of the structure of a culture dish according to the present invention;
FIG. 6 is a side view of a culture dish according to the utility model;
FIG. 7 is a schematic view of a culture dish lid of the present invention;
wherein, 1 represents a limiting hole, 2 represents a hydrophobic frame, 3 represents a glass slide body, 4 represents a handheld part, 5 represents an inclined plane, 6 represents a cell culture dish body, 7 represents a culture dish cover, 8 represents a grid and 9 represents a clapboard.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
As shown in fig. 1-7, a primary cardiomyocyte slide culture system comprises a slide and a square culture dish for accommodating the slide, wherein the slide comprises a slide body 3, a handheld portion 4 is arranged at the head of the slide body 3, a circular ring-shaped hydrophobic frame 2 is arranged on the upper surface of the slide body 3, a circular hole surrounded by the hydrophobic frame 2 is a limiting hole 1, an inclined plane 5 with a width of 5mm is arranged at the middle position of the top end of the handheld portion 4 of the slide, and the distance between the highest position of the inclined plane 5 and the bottom of the square culture dish is 1 mm. The square culture dish comprises a cell culture dish body 6 and a culture dish cover 7, wherein a partition plate 9 is arranged at the bottom of the cell culture dish body 6, and the cell culture dish body 6 is divided into cells 8 matched with the shapes of the glass slides by the partition plate 9. The hydrophobic frame 2 is a hydrophobic coating layer, the thickness of the coating layer is 5-25 μm, the width of the hydrophobic frame 2 is 3mm, the inner diameter of the limiting hole 1 is 12mm, and the volume of the limiting hole 1 is 500 μ L. Scales are arranged on the side wall of the partition board 9.
In the specific implementation: the size of the glass slide is the size of a conventional glass slide sold in the market, the size is 25.4 multiplied by 76.2mm, the thickness is 1.2-1.7 mm, 500 mu L of primary myocardial cell suspension can be contained in the limiting hole 1, and the primary myocardial cells can grow in a wall-pasting mode in the limiting hole 1. Except the head parts of the circular hydrophobic frame 2 and the glass slide body 3, other parts are transparent, so that the observation of an inverted microscope is facilitated. The front surface of the handheld part 4 of the glass slide is a frosted surface, so that marks can be written conveniently; the middle position of the top end of the glass slide holding part 4 is provided with an inclined plane 5 with the width of 5mm, the distance between the highest position of the inclined plane 5 and the bottom of the culture dish is 1mm, the tweezers can conveniently go deep into the glass slide to clamp, and the adsorption force between liquid at the bottom of the glass slide and the bottom of the culture dish is overcome.
The square culture dish for accommodating the glass slide comprises a cell culture dish body 6 and a culture dish cover 7, wherein 3 lattices 8 matched with the shape of the glass slide are arranged on the bottom surface of the cell culture dish body 6. The matching means that the size of the grid can ensure that the glass slide does not shake in the shallow groove, and can also ensure that a gap is reserved between the glass slide and the inner wall of the shallow groove, so that the glass slide can be conveniently taken out by a tool such as tweezers. The side wall of the clapboard 9 is provided with liquid volume scales, which is convenient for quantitatively adding the culture solution.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (5)
1. A primary cardiomyocyte slide culture system is characterized in that: including slide glass and the square culture dish that holds slide glass, slide glass includes slide glass body (3), the head of slide glass body (3) is equipped with handheld portion (4), the upper surface of slide glass body (3) is equipped with ring shape hydrophobic frame (2), the circular port that hydrophobic frame (2) enclosed is spacing hole (1), handheld portion (4) top intermediate position of slide glass is equipped with inclined plane (5), the highest point and the square culture dish bottom distance of inclined plane (5) are 1 mm.
2. A primary cardiomyocyte slide culture system according to claim 1, wherein: the square culture dish comprises a cell culture dish body (6) and a culture dish cover (7), a partition plate (9) is arranged at the bottom of the cell culture dish body (6), and the cell culture dish body (6) is divided into grids (8) matched with the shape of the glass slide by the partition plate (9).
3. A primary cardiomyocyte slide culture system according to claim 1, wherein: the water repellent frame (2) is a water repellent coating layer, the thickness of the coating layer is 5-25 mu m, the width of the water repellent frame (2) is 3mm, the inner diameter of the limiting hole (1) is 12mm, and the volume of the limiting hole (1) is 500 mu L.
4. A primary cardiomyocyte slide culture system according to claim 1, wherein: the width of the inclined plane (5) is 5 mm.
5. A primary cardiomyocyte slide culture system according to claim 2, wherein: scales are arranged on the side wall of the clapboard (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122463043.1U CN216445378U (en) | 2021-10-13 | 2021-10-13 | Primary myocardial cell slide culture system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122463043.1U CN216445378U (en) | 2021-10-13 | 2021-10-13 | Primary myocardial cell slide culture system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216445378U true CN216445378U (en) | 2022-05-06 |
Family
ID=81350519
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122463043.1U Active CN216445378U (en) | 2021-10-13 | 2021-10-13 | Primary myocardial cell slide culture system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216445378U (en) |
-
2021
- 2021-10-13 CN CN202122463043.1U patent/CN216445378U/en active Active
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231101 Address after: 050000 North Road 1, Wumashan Industrial Park, Zanhuang County, Shijiazhuang City, Hebei Province Patentee after: HEBEI ZB-GAMAY BIOLOGICAL TECHNOLOGY Co.,Ltd. Address before: No.19 Taiji Road, economic and Technological Development Zone, Handan City, Hebei Province Patentee before: HEBEI University OF ENGINEERING Patentee before: HEBEI LESHOU DUCK INDUSTRY Co.,Ltd. |