CN214097178U

CN214097178U - A micropore board that is used for convenient formation of image of zebra fish juvenile fish live body

Info

Publication number: CN214097178U
Application number: CN202022344704.4U
Authority: CN
Inventors: 刘静; 余伟师; 梁萌萌; 王小冬; 刘乐; 叶鑫
Original assignee: Zhejiang Saiweisi Biotechnology Co Ltd
Current assignee: Zhejiang Saiweisi Biotechnology Co Ltd
Priority date: 2020-10-20
Filing date: 2020-10-20
Publication date: 2021-08-31
Anticipated expiration: 2030-10-20

Abstract

The utility model provides a micropore plate for conveniently imaging the living body of the zebra fish juvenile fish, which comprises a glass slide, is horizontally placed and is used for bearing the zebra fish juvenile fish and a water film; the PDMS film naturally covers the glass slide; the thickness of the PDMS film is 1mm plus or minus 0.1 mm; the PDMS membrane is provided with a plurality of penetrating holes, the hole diameter of each hole is matched with the body length of the zebra fish juvenile fish, the holes and a glass slide below the holes form a sample groove, and the sample groove is used for placing the zebra fish juvenile fish and a water layer. The raw materials that above-mentioned scheme relates to acquire easily, and the equipment is convenient, has realized carrying out the convenient formation of image of live body simultaneously, the possibility of high-speed motion shooing to zebra fish juvenile fish, and experimental back zebra fish juvenile fish can use repeatedly. Meanwhile, the sample grooves are designed to be compatible with a multi-channel pipettor, and are convenient to use for drug testing.

Description

A micropore board that is used for convenient formation of image of zebra fish juvenile fish live body

Technical Field

The utility model relates to a biological assay field, especially a micropore board.

Background

The zebra fish is slightly fusiform in body extension, small and slightly sharp in head, short in kiss, covered with a plurality of deep blue longitudinal lines like zebra, and organized compared with silvery white or golden yellow longitudinal lines arranged alternatively. When the zebra fish swim in the aquarium in groups, the zebra fish are similar to the zebra fish which run on the African grassland, so the zebra fish are attractive.

The zebra fish and the human gene have high similarity of 87 percent and have prominent advantages as model organisms, so the experimental result is also suitable for the human body in most cases.

In observation experiments of the form and the movement behavior of the zebra fish juvenile fish, a clear microscopic image needs to be shot for the juvenile fish living body, and a thin water layer needs to be created to enable the whole fish body to be kept on the same focusing plane. The above scenario suffers from at least the following problems in the current processing:

1. common sample containers, such as 96-well plates, are commonly used because their deep depth does not provide a uniform thin water layer to support the living zebrafish.

2. In the prior art, young fishes are fixed in gel for photographing, but the method is tedious and time-consuming, a sample cannot be used after photographing, and dynamic photographing is not supported.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a micropore board that is used for the convenient formation of image of zebra fish live body for solve the unable fine technological problem of realizing that zebra fish juvenile fish developments were shot of current means.

To achieve the above objective, the present invention provides the following technical solutions:

a micropore board that is used for convenient formation of image of zebra fish juvenile fish live body, include

The glass slide is horizontally placed and used for supporting the zebra fish juvenile fish and the water film;

the PDMS film naturally covers the glass slide; the thickness of the PDMS film is 1mm plus or minus 0.1 mm; the PDMS membrane is provided with a plurality of penetrating holes, the hole diameter of each hole is matched with the body length of the zebra fish juvenile fish, the diameter of each hole is 5mm +/-0.1 mm, the holes and a glass slide below the holes form a sample tank, and the sample tank is used for placing the zebra fish juvenile fish and a water layer.

Further, in the present invention, the width of the PDMS film is the same as the width of the glass slide, and the length of the PDMS film is smaller than the length of the glass slide.

Further, in the utility model discloses in, the sample groove is evenly arranged along length and width direction on the PDMS membrane, and wherein the hole heart interval between the adjacent sample groove is 9 mm.

Further, in the utility model discloses in, the water layer height in the micropore board is 1mm 0.1 mm.

Has the advantages that:

according to the technical scheme provided by the utility model, the technical scheme provides a supply zebra fish juvenile fish to carry out the equipment of developments shooing. This equipment is by slide glass and PDMS film formation sample groove, put into zebra fish juvenile fish and the thin water layer of homogeneity in the sample groove, because the nature that the PDMS material has adsorbs in the performance on smooth glass surface, make sample groove stable in structure, and the sample groove height of 1mm left and right sides that the PDMS film provided makes the water film that can form the film appearance, provide certain activity space of zebra fish on the one hand, on the other hand provides the environment that the zebra fish lives, realized carrying out the convenient formation of image of live body simultaneously to the zebra fish juvenile fish, the possibility of high-speed motion shooing, experimental back zebra fish juvenile fish can be used repeatedly. Meanwhile, the sample grooves are designed to be compatible with a multi-channel pipettor, and are convenient to use for drug testing.

The raw materials that above-mentioned scheme relates to acquire easily, and the equipment is convenient, can shoot and use the sample repeatedly by the developments, has good application prospect.

It should be understood that all combinations of the foregoing concepts and additional concepts described in greater detail below can be considered as part of the inventive subject matter of the present disclosure unless such concepts are mutually inconsistent.

The foregoing and other aspects, embodiments and features of the present teachings can be more fully understood from the following description taken in conjunction with the accompanying drawings. Additional aspects of the present invention, such as features and/or advantages of exemplary embodiments, will be apparent from the description which follows, or may be learned by practice of the specific embodiments in accordance with the teachings of the present invention.

Drawings

The drawings are not intended to be drawn to scale. In the drawings, each identical or nearly identical component that is illustrated in various figures may be represented by a like numeral. For purposes of clarity, not every component may be labeled in every drawing. Embodiments of various aspects of the present invention will now be described, by way of example, with reference to the accompanying drawings, in which:

fig. 1 is a schematic view of the overall structure of a microporous plate according to the present invention;

fig. 2 is a front view of the micro-porous plate of the present invention.

In the figures, the meaning of the reference numerals is as follows:

slide 1, PDMS membrane 2, sample groove 3.

Detailed Description

For a better understanding of the technical content of the present invention, specific embodiments are described below in conjunction with the accompanying drawings.

In this disclosure, aspects of the present invention are described with reference to the accompanying drawings, in which a number of illustrative embodiments are shown. Embodiments of the present disclosure are not necessarily intended to include all aspects of the invention. It should be appreciated that the various concepts and embodiments described above, as well as those described in greater detail below, may be implemented in any of numerous ways, as the disclosed concepts and embodiments are not limited to any implementation. Additionally, some aspects of the present disclosure may be used alone or in any suitable combination with other aspects of the present disclosure.

Summary of the application

The zebra fish juvenile fish needs to be photographed and dynamically observed in a laboratory, and a thin film water layer needs to be provided for the zebra fish juvenile fish, so that the zebra fish juvenile fish can keep alive and can move in a small range. However, in all the existing test equipment, special equipment capable of directly realizing the functions is not available, a common 96-hole microporous plate is large in space depth, zebra fish bodies are difficult to keep on the same horizontal plane and cannot be observed in a focusing mode, and the zebra fish bodies are directly fixed by gel and then observed, so that the zebra fish bodies lose activity and cannot complete a dynamic observation task, and the zebra fish bodies after being used cannot be reused.

In view of the above technical problems, the technical solution provided by the present application has the following general idea:

The glass slide 1 is horizontally placed and used for supporting the zebra fish juvenile fish and the water film;

the PDMS film 2 naturally covers the glass slide 1; the thickness of the PDMS membrane 2 is 1mm plus or minus 0.1 mm; the PDMS membrane 2 is provided with a plurality of penetrating holes, the hole diameter of each hole is matched with the body length of the zebra fish juvenile fish, the diameter of each hole is 5mm +/-0.1 mm, the holes and a glass slide 1 below the holes form a sample groove 3, and the sample groove 3 is used for placing the zebra fish juvenile fish and a water layer.

Having thus described the general principles of the present application, various non-limiting embodiments thereof will now be described in detail with reference to the accompanying drawings.

As shown in fig. 1-2, is a schematic structural diagram of the present invention. The PDMS membrane 2 is naturally covered with the glass slide 1, the PDMS membrane 2 is a hydrophobic organic silicon material, and the material has biocompatibility, is easy to prepare and assemble, can be produced in batches and is low in cost. The sample groove is naturally adsorbed on the surface of the glass slide 1 to form a state which is not easy to move, so that the glass slide 1 and the holes on the PDMS form the sample groove 3 which is stable in structure and not easy to move. Since the height of the zebra fish juvenile fish or the thickness of the zebra fish juvenile fish is less than 1mm, the water layer of about 1mm in the sample tank 3 can provide the zebra fish juvenile fish with a certain movement space, the space can allow the zebra fish to be in a free movement state, and the life of the zebra fish can be maintained.

In order to obtain a water layer of 1 mm. + -. 0.1mm, the PDMS membrane 2 is designed to have a thickness corresponding to the target water layer, and a small amount of culture medium is dropped into the sample well 3 via a dropper to form a uniform thin water layer. When the thickness of the water layer is greatly different from that of the PDMS membrane 2, water is continuously added, when the upper edge of the water layer reaches the upper surface of the PDMS membrane 2, the thickness of the water layer is equal to that of the PDMS membrane 2, and when the edge of the water layer reaches the upper surface of the PDMS membrane 2, the height of the center of the water layer is the sum of the thickness of the PDMS membrane 2 and the height formed under the action of a certain amount of surface tension. Since the PDMS film 2 itself has a very small thickness, the water layer is substantially a uniform thin film. Above-mentioned thin water layer that thickness is fairly restricts a plurality of zebra fishes of shooing simultaneously in same focus plane, and the sound is taken a picture to the state of being convenient for.

Further, for easy handling during the test, the PDMS film 2 is not the same size as the glass slide 1, and a part of the area on the glass slide 1 needs to be left as a place for the experimenter to take. As shown in FIG. 2, the glass slide 1 is 76mm long, 26mm wide and 1mm high, the PDMS film is 60mm long, 26mm wide and 1mm high, the width of the PDMS film 2 is the same as that of the glass slide 1, and the length of the PDMS film 2 is smaller than that of the glass slide 1. The right part of the slide 1 in the figure is 16mm long and 26mm wide, which is the area where the laboratory worker takes the slide 1.

Further, preferably, the above-described device can also be used for drug testing, for which the sample wells 3 are uniformly arranged on the PDMS film 2 in the length and width directions, wherein the hole center-to-center distance between adjacent sample wells 3 is 9 mm. The above-mentioned hole of evenly arranging, the diameter is 5mm for a plurality of samples can be observed simultaneously, and compatible with current pipettor just, conveniently add the medicine with the pipettor in to a plurality of sample groove 3, carry out the drug testing to a plurality of samples simultaneously.

The utility model discloses an above-mentioned concrete embodiment provides a new micropore board that is used for zebra fish juvenile fish formation of image, and it realizes the thin water layer about 1mm effectively, provides certain activity space of zebra fish on the one hand, and on the other hand provides the environment that zebra fish survived, has realized carrying out the convenient formation of image of live body, high-speed motion and has shot and the possibility of using repeatedly to zebra fish juvenile fish. Meanwhile, the sample grooves 3 are designed to be compatible with a multi-channel pipettor, and are convenient to use for drug testing. The raw materials that above-mentioned scheme relates to acquire easy cost of manufacture low, equipment and convenient operation, support dynamic photographing and use the sample repeatedly, have good application prospect.

Although the present invention has been described with reference to the preferred embodiments, it is not intended to limit the present invention. The present invention is intended to cover by those skilled in the art various modifications and adaptations of the invention without departing from the spirit and scope of the invention. Therefore, the protection scope of the present invention is subject to the claims.

Claims

1. The utility model provides a micropore board that is used for convenient formation of image of zebra fish juvenile fish live body which characterized in that: comprises that

2. The microplate for convenient living body imaging of zebra fish juvenile fish of claim 1, wherein: the width of the PDMS film is the same as that of the glass slide, and the length of the PDMS film is smaller than that of the glass slide.

3. The microplate for convenient living body imaging of zebra fish juvenile fish of claim 2, wherein: the sample grooves were uniformly arranged on the PDMS film in the length and width directions, wherein the hole center distance between adjacent sample grooves was 9 mm.

4. The microplate for convenient living body imaging of zebra fish juvenile fish of claim 3, wherein: the height of the water layer in the microporous plate is 1mm plus or minus 0.1 mm.