CN218811657U - 3D cell culture matrigel protection trades liquid device - Google Patents

Abstract

The utility model provides a 3D cell culture matrigel protection trades liquid device, be fixed in including tray and tiling the body that absorbs water of making by the material that absorbs water on the tray, the surface that the body that absorbs water has a plurality of banding flexibility probes that absorb water, the flexibility probe that absorbs water with the body contact that absorbs water. The beneficial effects of the utility model are that can absorb original culture solution totally under the prerequisite of not destroying the cell culture medium, be convenient for change new culture solution, let the cell continue healthy growth, its structural design is reasonable, and convenient to use trades liquid efficiently.

Description

3D cell culture matrigel protection trades liquid device

Technical Field

The utility model relates to a 3D cell culture matrigel protection trades liquid device.

Background

In drug research and various kinds of mechanical research, various kinds of cells separated from human bodies or animal bodies need to be cultured in vitro, and the growth state of the cells in the bodies can be better reduced through three-dimensional 3D cell culture, so that reliable experimental data can be provided. The 3D culture of cells usually takes matrigel or cell scaffold material as a medium, so that the cells are suspended and grown in the medium, and meanwhile, culture solution is required to be replaced periodically, so as to achieve the purpose of continuously supplementing growth nutrients. The existing operation method is to use a machine vacuum pump or an operator to manually suck out the original culture solution through a pipette gun. The defect is that the 3D hydrogel and other media are softer and even have little fluidity compared with solid substances, the operation of sucking out the original culture solution by using a traditional machine or a manual method is adopted, and if the force and the position are improperly controlled, the matrigel or the cell scaffold is easily damaged, so that the whole cell culture sample fails; if some original culture solution remains in order not to destroy the culture system, the calculation of volume and component concentration of the whole experiment culture solution is influenced after adding new culture solution, resulting in experiment errors.

Disclosure of Invention

The to-be-solved problem of the utility model is to provide a 3D cell culture matrigel protection trades liquid device can absorb original culture solution totally under the prerequisite of not destroying the cell culture medium, is convenient for change new culture solution, lets the cell continue healthy growth.

In order to solve the technical problem, the utility model adopts the technical scheme that: the utility model provides a 3D cell culture matrigel protection trades liquid device, includes the tray and the tiling is fixed in the body that absorbs water of making by the material that absorbs water on the tray, the surface that absorbs water the body has a plurality of banding flexible probes that absorb water, flexible probe that absorb water with the body contact that absorbs water.

Optionally, the water-absorbing body is made of water-absorbing sponge.

Optionally, the flexible water absorption probe is water absorption filter paper.

Optionally, the flexible water absorption probes are distributed on the water absorption body in a matrix manner.

Optionally, the length of the flexible water absorption probe is 0.5cm-1.5cm.

Optionally, the length of the flexible water absorption probe is 1cm.

Optionally, the water absorption body further comprises a protection frame, the protection frame is a rectangular frame, the protection frame can be placed on the tray, and the hollow area inside the protection frame at least can cover the area, provided with the flexible water absorption probe, of the water absorption body;

the novel protective frame is characterized in that two connecting rods which are oppositely arranged are arranged in a hollow area in the protective frame, a plurality of shift levers are arranged between the two connecting rods at intervals, two sliding holes are formed in two frames, parallel to the connecting rods, of the protective frame, each connecting rod is provided with a sliding plate corresponding to the sliding hole, the sliding plates are limited in the sliding holes and can drive the connecting rods to slide in the protective frame along the direction of the sliding holes, fastening bolts are arranged on the sliding plates, and the fastening bolts can limit the sliding of the sliding plates.

Optionally, the protective frame is hinged to the tray through a hinge, and a detachable cover plate is arranged on the protective frame.

The utility model has the advantages and positive effects be: by adopting the technical scheme, the original culture solution can be absorbed cleanly on the premise of not damaging a cell culture medium, the new culture solution is convenient to replace, cells continue to grow healthily, and the cell culture medium has the advantages of reasonable structural design, convenience in use and high solution replacement efficiency.

Drawings

FIG. 1 is a schematic diagram of the overall structure of an embodiment of the present invention;

FIG. 2 is a schematic structural diagram of another embodiment of the present invention;

FIG. 3 is a schematic view of the protective frame of FIG. 2 after being covered;

FIG. 4 is an enlarged partial schematic view at A of FIG. 3;

in the figure: 1-a tray; 2-water-absorbing sponge; 3-absorbent filter paper; 4-a protective frame; 5-a hinge; 6-a deflector rod; 7-a connecting rod; 8-a slide hole; 9-a sliding plate; 10-fastening bolts.

Detailed Description

The present invention will be described in further detail with reference to specific examples. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, are not to be construed as limiting the present invention. In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; the two components can be directly connected with each other or indirectly connected with each other through an intermediate medium, and the two components can be communicated with each other. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.

As shown in figure 1, the utility model provides a 3D cell culture matrigel protection trades liquid device, including tray 1 and the sponge 2 that absorbs water that the peaceful shop is fixed in on tray 1, the sponge 2 that absorbs water's surface has many of matrix distribution to absorb water filter paper 3. Wherein the water-absorbing sponge 2 can be fixed on the tray 1 by adopting an adhesive way. The using mode of this structure is, take tray 1 in the surface of cell culture board, the sponge 2 that absorbs water this moment has the one side of absorbing water filter paper 3 downwards, the lower extreme of absorbing water filter paper 3 is perpendicular downwards under the action of gravity, it stretches into each downthehole on the cell culture board respectively to absorb water filter paper 3, with this culture solution suction that needs the replacement in the hole, the liquid that absorbs gets into in the sponge 2 that absorbs water through absorbing water filter paper 3, because absorbing water filter paper 3 is comparatively soft, even touch downthehole matrigel can not lead to the fact destruction to it yet, static 1 minute, take the device off from the cell culture board after waiting to absorb downthehole nutrient solution, it can to abandon the sponge 2 that absorbs full of liquid.

The sizes of the tray 1 and the water-absorbing sponge 2 can be set according to the specification of a corresponding used bacterial culture plate, taking a 96-hole bacterial culture plate as an example, the specification of the plate is 125mm in length, 84mm in width, 15mm in height and 7mm in hole diameter, the water-absorbing sponge 2 on the tray 1 can be set to be the same as a 96-hole cell culture plate (125mm, 84mm) in plane size, the thickness of the water-absorbing sponge 2 is 1mm, the water-absorbing capacity of the water-absorbing sponge is not lower than the capacity (1 ml) in one 96-hole plate, the length of the water-absorbing filter paper 3 is 1cm, and the matrix distribution position of the water-absorbing filter paper 3 corresponds to the hole position (8 rows and 12 rows) on the 96-hole cell culture plate. The sizes of the water-absorbing sponges 2 and the lengths and the distribution positions of the water-absorbing filter papers 3 with different sizes are set for cell culture plates with different specifications.

Wherein, the water-absorbing sponge 2 and the water-absorbing filter paper 3 can be replaced by other materials which can achieve the asepsis and have the water-absorbing capacity.

The device replaces original mechanical negative pressure suction method with the physical siphon method, reduces the external force action of the outside on the 3D cell culture system to the minimum, fully absorbs the culture solution to be replaced to the maximum extent on the premise of not damaging matrigel or a cell scaffold system, simultaneously avoids experimental errors caused by the difference of the volumes of the culture solution among different holes, and ensures the success rate and the data quality of the experiment.

In the structure of the above embodiment, the water-absorbing filter paper 3 is relatively soft and is easily broken or bent during packaging and transportation, which affects subsequent use, and in order to overcome this defect, the above structure is further improved, specifically as follows:

as shown in fig. 2-4, the water absorption sponge further comprises a protective frame 4, the protective frame 4 is a rectangular frame, the protective frame 4 can be placed on the tray, and the hollow area inside the protective frame 4 at least can cover the area, with the water absorption filter paper 3, on the water absorption sponge 2;

two connecting rods 7 which are oppositely arranged are arranged in a hollow-out area in the protective frame 4, a plurality of shift levers 6 are arranged between the two connecting rods 7 at intervals, two sliding holes 8 are formed in two frames, parallel to the connecting rods 7, of the protective frame 4, a sliding plate 9 corresponding to each sliding hole 8 is arranged on each connecting rod 7, each sliding plate 9 is limited in each sliding hole 8 and can drive the connecting rods 7 to slide in the protective frame 4 along the direction of the corresponding sliding holes 8, fastening bolts 10 are arranged on the sliding plates 9, and the sliding of the sliding plates 9 can be limited by screwing the fastening bolts 10.

The protective frame 4 is hinged with the tray 1 through a hinge 5.

Before the encapsulation, with before 4 lids of protective frame fit on tray 1, at first sliding connecting rod 7, adjust its position, make every driving lever 6 all be located between two adjacent lines of absorbent filter paper 3 in the matrix after the lid closes, then promote driving lever 6 towards one side, make driving lever 6 and absorbent sponge 3 contact, absorbent sponge 3 is towards one side buckling under the oppression of driving lever 6 this moment, the tip that makes absorbent sponge 3 is located inside protective frame 4, because protective frame 4's protection, the encapsulation membrane can not oppress absorbent filter paper 3 during the encapsulation, filter paper 3 has formed better protection to absorbing, even if jolting slightly on the way like this can not make absorbent filter paper 3 buckle, also can set up the detachable apron on protective frame 4, further play the guard action.

When in use, the deflector rod 6 is pushed towards the opposite direction, and the water absorption filter paper 3 stands up under the push of the deflector rod 6, thereby being convenient for use.

The embodiments of the present invention have been described in detail, but the description is only for the preferred embodiments of the present invention and should not be construed as limiting the scope of the present invention. All the equivalent changes and improvements made according to the application scope of the present invention should still fall within the patent coverage of the present invention.

Claims (8)

1. The utility model provides a 3D cell culture matrigel protection trades liquid device which characterized in that: the water absorption device comprises a tray and a water absorption body which is tiled and fixed on the tray and is made of water absorption materials, wherein the surface of the water absorption body is provided with a plurality of strip-shaped flexible water absorption probes, and the flexible water absorption probes are in contact with the water absorption body.

2. The 3D cell culture matrigel protection fluid exchange device of claim 1, characterized in that: the water absorption body is made of water absorption sponge.

3. The 3D cell culture matrigel protection fluid exchange device of claim 1, characterized in that: the flexible water absorption probe is water absorption filter paper.

4. The 3D cell culture matrigel protection and fluid replacement device of any one of claims 1-3, wherein: the flexible water absorption probes are distributed on the water absorption body in a matrix manner.

5. The 3D cell culture matrigel protection and fluid exchange device of claim 4, characterized in that: the length of the flexible water absorption probe is 0.5cm-1.5cm.

6. The 3D cell culture matrigel protection and fluid exchange device of claim 5, characterized in that: the length of the flexible water absorption probe is 1cm.

7. The 3D cell culture matrigel protection fluid change device of claim 6, characterized in that: the water absorption body is characterized by further comprising a protection frame, wherein the protection frame is a rectangular frame and can be placed on the tray, and the hollow area inside the protection frame at least can cover the area, provided with the flexible water absorption probe, of the water absorption body;

the novel protective frame is characterized in that two connecting rods which are oppositely arranged are arranged in a hollow area in the protective frame, a plurality of shift levers are arranged between the two connecting rods at intervals, two sliding holes are formed in two frames, parallel to the connecting rods, of the protective frame, each connecting rod is provided with a sliding plate corresponding to the sliding hole, the sliding plates are limited in the sliding holes and can drive the connecting rods to slide in the protective frame along the direction of the sliding holes, fastening bolts are arranged on the sliding plates, and the fastening bolts can limit the sliding of the sliding plates.

8. The 3D cell culture matrigel protection fluid exchange device of claim 7, characterized in that: the protective frame is hinged to the tray through a hinge, and a detachable cover plate is arranged on the protective frame.

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