CN213624190U - Hepatocyte culture and storage device - Google Patents
Hepatocyte culture and storage device Download PDFInfo
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- CN213624190U CN213624190U CN202022333724.1U CN202022333724U CN213624190U CN 213624190 U CN213624190 U CN 213624190U CN 202022333724 U CN202022333724 U CN 202022333724U CN 213624190 U CN213624190 U CN 213624190U
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Abstract
The utility model discloses a hepatocyte culture and storage device, including the storage box, two culture box of fixedly connected with between the inner wall of storage box both sides, two the first fixed plate of upper surface both ends difference fixedly connected with and the second fixed plate of culture box, the bar mouth has all been seted up to the inside of first fixed plate and second fixed plate rack and one side outer wall, two the equal fixedly connected with backup pad in one side of rack, two the one end that the rack was kept away from to the backup pad all runs through the bar mouth and extends to outside and common fixedly connected with storage frame, one side fixedly connected with motor of storage frame is kept away from to the second fixed plate. The utility model discloses in, can cultivate simultaneously and save multiunit cell, unified operation need not to shift, is convenient for carry out the inside maintenance clearance and the replenishment of nutrient solution, and it is comparatively simple and convenient to operate, can effectively disinfect simultaneously and make inside keep sealed, improves the cultivation effect of cell.
Description
Technical Field
The utility model relates to a cell culture technical field especially relates to a liver cell culture and storage device.
Background
In vitro culture of cells and tissues has become an indispensable matter for life research and practice, and plays an important role in regenerative medicine and disease treatment. The cell types in culture are numerous, ranging from viruses to bacteria and fungi, and from human cells to animal and plant cells, and cell culture is an essential process for the entire biotechnology.
Need save it among the hepatocyte culture process, in order to subsequent operation and research, but most culture vessel do not possess the storage function, need regularly carry out the change replenishment of inside culture solution, and save in transferring to dedicated storage container, cultivate multiunit experiment group among the experimental operation and carry out the comparison statistics, need change the cleaning operation to each experimental group of cultivateing, work load is great, cause the hourglass easily, when carrying out the during operation of cultivateing and storage simultaneously, need carry out the transfer of cell, lead to easily that the internal environment receives the bacterial erosion, influence whole experiment effect.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the defects existing in the prior art and providing a hepatocyte culturing and storing device.
In order to achieve the above purpose, the utility model adopts the following technical scheme: a hepatocyte culture and storage device comprises a storage box, wherein two culture boxes are fixedly connected between the inner walls of two sides of the storage box, two ends of the upper surface of each culture box are respectively and fixedly connected with a first fixing plate and a second fixing plate, racks are arranged inside the first fixing plate and the second fixing plate, a strip-shaped opening is formed in the outer wall of one side of each culture box, a support plate is fixedly connected to one side of each rack, one ends of the two support plates, far away from the racks, penetrate through the strip-shaped openings and extend to the outside, a storage rack is fixedly connected with the support plates together, a motor is fixedly connected to one side of the second fixing plate, far away from the storage rack, the driving end of the motor penetrates through the second fixing plate and is fixedly connected with a rotating shaft, a first gear and a second gear are respectively and fixedly connected to two ends of the outer surface of the rotating shaft, a, it is a plurality of the roof downwardly extending who cultivates the box all runs through in the bottom of cultivate the pipe extends inside, the inside of cultivateing the box is provided with the culture tank, bottom wall fixedly connected with ultraviolet ray disinfection lamp in the culture tank, and cultivate and all be provided with the sealing strip between box both sides inner wall and the culture tank.
As a further description of the above technical solution:
the lower surface of the two racks is fixedly connected with connecting plates, and one sides, far away from the storage rack, of the two connecting plates are respectively connected with the side walls of the first fixing plate and the second fixing plate in a sliding mode.
As a further description of the above technical solution:
the one end that the motor was kept away from in the pivot runs through the outer wall extension of first and first fixed plate and inside and first fixed plate inside wall rotation connection.
As a further description of the above technical solution:
the first gear is located inside the second fixing plate, the second gear is located inside the first fixing plate, and the first gear and the second gear are respectively meshed with the two racks.
As a further description of the above technical solution:
two the rack is kept away from the one end of first fixed plate, second fixed plate and is all run through first fixed plate, second fixed plate roof and upwards extend to the outside.
As a further description of the above technical solution:
it is a plurality of the surface of culture tube all is fixed to have cup jointed the spacing ring, and a plurality of spacing rings all are located the upper surface of holding tank, and is a plurality of the bottom of culture tube all runs through and has seted up two inlets, and the position that is close to the inlet between the inner wall of culture tube both sides is provided with the micropore board.
As a further description of the above technical solution:
the inside nutrient solution that is provided with of culture tank, and the equal fixedly connected with slider in culture tank lower surface both sides, and culture tank through two sliders and culture box inner wall sliding connection.
The utility model discloses following beneficial effect has:
1. this liver cell culture and storage device through culture tube, micropore board, motor, storage box, rack, storage frame, first gear and the second gear that sets up, can cultivate simultaneously and store multiunit cell, need not to carry out the cell and shift, and the unified operation of multiunit cell is convenient for carry out the inside maintenance clearance and the replenishment of nutrient solution, and it is comparatively simple and convenient to operate, need not to operate one by one, effectively promotes work efficiency.
2. This liver cell culture and storage device, through culture box, culture tank, ultraviolet ray disinfection lamp, spacing ring and the sealing strip that sets up, be convenient for carry out the replenishment of nutrient solution and change, can effectively disinfect and make inside keep sealed, effectively maintain the cultivation environment, improve the cultivation effect of cell.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a partial sectional top view of the storage box of the present invention;
FIG. 3 is a sectional view of the culture box of the present invention.
Illustration of the drawings:
1. a storage box; 2. a culture box; 3. a first fixing plate; 4. a motor; 5. a first gear; 6. a second fixing plate; 7. a rotating shaft; 8. a second gear; 9. a rack; 10. a connecting plate; 11. a strip-shaped opening; 12. a support plate; 13. a storage rack; 14. a storage tank; 15. a culture tube; 16. a limiting ring; 17. a culture tank; 18. a liquid inlet; 19. an ultraviolet disinfection lamp; 20. a slider; 21. a sealing strip; 22. a microplate.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Referring to fig. 1-3, the present invention provides an embodiment: a hepatocyte culture and storage device comprises a storage box 1, wherein two culture boxes 2 are fixedly connected between the inner walls of two sides of the storage box 1, two ends of the upper surface of each culture box 2 are respectively and fixedly connected with a first fixing plate 3 and a second fixing plate 6, racks 9 are respectively arranged inside the first fixing plate 3 and the second fixing plate 6, a strip-shaped opening 11 is respectively arranged on the outer wall of one side of each culture box, one side of each rack 9 is respectively and fixedly connected with a supporting plate 12, one ends of the two supporting plates 12, far away from the racks 9, extend to the outside through the strip-shaped openings 11 and are jointly and fixedly connected with a storage rack 13, one side of the second fixing plate 6, far away from the storage rack 13, is fixedly connected with a motor 4, a driving end of the motor 4 penetrates through the second fixing plate 6 and is fixedly connected with a rotating shaft 7, two ends of the outer surface of the rotating shaft 7, inside all being provided with culture tube 15 of a plurality of holding tanks 14, inside the roof downwardly extending who cultivates box 2 all runs through in the bottom of a plurality of culture tubes 15, the inside of cultivateing box 2 is provided with culture tank 17, and bottom wall fixedly connected with ultraviolet ray disinfection lamp 19 in culture tank 17, and all be provided with sealing strip 21 between culture tank 17 and the inner wall of 2 both sides of box and the cultivation tank.
The lower surfaces of the two racks 9 are fixedly connected with connecting plates 10, and one sides, far away from the storage rack 13, of the two connecting plates 10 are respectively in sliding connection with the side walls of the first fixing plate 3 and the second fixing plate 6, so that the racks 9 can be kept stable and can move directionally, and the storage rack 13 is driven to lift.
The one end that motor 4 was kept away from to pivot 7 runs through the outer wall extension of first and first fixed plate 3 and inside and first fixed plate 3 inside wall and rotates and be connected, makes pivot 7 stable connection.
One end of each of the two racks 9, which is far away from the first fixing plate 3 and the second fixing plate 6, extends upwards to the outside through the top wall of the first fixing plate 3 and the top wall of the second fixing plate 6, so that a certain moving space is provided, and the racks 9 can move and rise to a certain height.
The outer surface of a plurality of culture tubes 15 is all fixed has cup jointed spacing ring 16, and a plurality of spacing rings 16 all are located the upper surface of holding tank 14, make culture tube 15 and storage frame 13 remain stable, two inlet 18 have all been seted up to the bottom of a plurality of culture tubes 15 through running through, make nutrient solution can get into inside culture tube 15, carry out the cultivation of hepatocyte, and the position that is close to inlet 18 between the inner wall of culture tube 15 both sides is provided with micropore board 22, can bear the weight of hepatocyte, prevent to fall into inside culture tank 17 when making it dip in nutrient solution.
The inside nutrient solution that is provided with of culture tank 17, and the equal fixedly connected with slider 20 in culture tank 17 lower surface both sides, and culture tank 17 through two sliders 20 with cultivate 2 inner diapire sliding connection of box, can take culture tank 17 out and carry out the replenishment change of nutrient solution, the simple operation.
The working principle is as follows: when the hepatocyte culture and storage device is used, hepatocytes to be cultured are placed on the upper surface of a microporous plate 22, a plurality of culture tubes 15 are arranged and then are placed in a storage tank 14 for fixation, after the placement is finished, a motor 4 is started, a rotating shaft 7 rotates to drive a first gear 5 and a second gear 8 to rotate, two racks 9 are driven to descend to drive the bottoms of the culture tubes 15 to descend into a culture tank 17, the hepatocytes are immersed in a nutrient solution, when observation and recording are needed, the culture tubes 15 can be taken down for operation, when nutrient solution is supplemented, the motor 4 is driven to turn over, the first gear 5 and the second gear 8 rotate to drive the culture tubes 15 to ascend and separate from the culture box 2, the culture tank 17 is pulled to separate one end of the culture tubes from the interior of the culture box 2 for liquid supplementation, an opening and closing door on the side wall of a storage box 1 is closed, the inside is sealed and stored.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.
Claims (7)
1. A hepatocyte culture and storage device, includes storage box (1), its characterized in that: two culture boxes (2) fixedly connected with between the inner walls of two sides of the storage box (1), two culture boxes (2) are respectively fixedly connected with a first fixing plate (3) and a second fixing plate (6) at the two ends of the upper surface of each culture box (2), a rack (9) and a bar-shaped opening (11) are formed in the first fixing plate (3) and the second fixing plate (6) respectively, two bar-shaped openings (11) are formed in the outer wall of one side of the rack (9) respectively, a supporting plate (12) and a supporting plate (12) are fixedly connected with one side of the supporting plate (12) far away from the rack (9), the bar-shaped opening (11) is penetrated through to extend to the outside, the storage frame (13) is fixedly connected with the storage frame (13) together, the motor (4) is far away from the second fixing plate (6) at one side of the second fixing plate (6), a first gear (5) and a second gear (8) are fixedly connected with the two ends of the Storage frame (13) inside runs through and has seted up a plurality of holding tanks (14), and is a plurality of inside culture tube (15) that all is provided with of holding tank (14), and is a plurality of the roof downwardly extending who cultivates box (2) is all run through to the bottom of culture tube (15) inside, the inside of cultivateing box (2) is provided with culture tank (17), bottom wall fixedly connected with ultraviolet ray disinfection lamp (19) in culture tank (17), and all be provided with between culture tank (2) both sides inner wall and the culture tank (17) sealing strip (21).
2. The hepatocyte culture and storage device of claim 1, wherein: the lower surface of the two racks (9) is fixedly connected with a connecting plate (10), and one side, far away from the storage rack (13), of the two connecting plates (10) is respectively connected with the side walls of the first fixing plate (3) and the second fixing plate (6) in a sliding mode.
3. The hepatocyte culture and storage device of claim 1, wherein: one end of the rotating shaft (7) far away from the motor (4) penetrates through the outer wall of the first fixing plate (3) to extend to the inside to be rotatably connected with the inner side wall of the first fixing plate (3).
4. The hepatocyte culture and storage device of claim 1, wherein: the first gear (5) is located inside the second fixing plate (6), the second gear (8) is located inside the first fixing plate (3), and the first gear (5) and the second gear (8) are respectively meshed with the two racks (9).
5. The hepatocyte culture and storage device of claim 1, wherein: two the rack (9) are kept away from the one end of first fixed plate (3), second fixed plate (6) and are all run through first fixed plate (3), second fixed plate (6) roof and upwards extend to the outside.
6. The hepatocyte culture and storage device of claim 1, wherein: it is a plurality of the surface of culture tube (15) all is fixed to have cup jointed spacing ring (16), and a plurality of spacing ring (16) all are located the upper surface of holding tank (14), and is a plurality of two inlet (18) have all been run through to the bottom of culture tube (15), and the position that is close to inlet (18) between culture tube (15) both sides inner wall is provided with micropore board (22).
7. The hepatocyte culture and storage device of claim 1, wherein: the culture tank (17) is internally provided with nutrient solution, the two sides of the lower surface of the culture tank (17) are fixedly connected with sliding blocks (20), and the culture tank (17) is connected with the inner bottom wall of the culture box (2) in a sliding mode through the two sliding blocks (20).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022333724.1U CN213624190U (en) | 2020-10-19 | 2020-10-19 | Hepatocyte culture and storage device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022333724.1U CN213624190U (en) | 2020-10-19 | 2020-10-19 | Hepatocyte culture and storage device |
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| Publication Number | Publication Date |
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| CN213624190U true CN213624190U (en) | 2021-07-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202022333724.1U Active CN213624190U (en) | 2020-10-19 | 2020-10-19 | Hepatocyte culture and storage device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114806832A (en) * | 2022-05-25 | 2022-07-29 | 深圳市华晨阳科技有限公司 | Small-size negative pressure infectivity pathology for experimental study preserves culture apparatus |
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2020
- 2020-10-19 CN CN202022333724.1U patent/CN213624190U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114806832A (en) * | 2022-05-25 | 2022-07-29 | 深圳市华晨阳科技有限公司 | Small-size negative pressure infectivity pathology for experimental study preserves culture apparatus |
| CN114806832B (en) * | 2022-05-25 | 2022-09-02 | 深圳市华晨阳科技有限公司 | Negative pressure infectious pathology preservation incubator for small-size experimental study |
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