CN211847902U

CN211847902U - Sterile sampling device

Info

Publication number: CN211847902U
Application number: CN202020186371.4U
Authority: CN
Inventors: 章文劼
Original assignee: Jiangsu Haifei Biotechnology Co ltd
Current assignee: Jiangsu Haifei Biotechnology Co ltd
Priority date: 2020-02-19
Filing date: 2020-02-19
Publication date: 2020-11-03
Anticipated expiration: 2030-02-19

Abstract

The utility model discloses an aseptic sampling device, wherein a sampling port comprises a base flange and a gland, and a sampling valve comprises a mounting plate and a sampling valve body; the base flange is provided with a counter bore from the outer end face of the base flange, a gradually expanded horn-shaped through hole is formed from the bottom of the counter bore to the inner end face of the base flange, and the base flange is fixedly installed with the fermentation tank opening from the inner side of the base flange; the sampling valve body is provided with a circulation hole, a steam pipeline and a sample outlet pipeline which are communicated with the inner cavity, a movable plunger is arranged in the inner cavity, an annular gap for communicating the steam pipeline and the sample outlet pipeline is arranged between the movable plunger and the inner cavity, and the sampling valve body is fixedly arranged on the mounting plate in a penetrating way from the inner side of the sampling valve body; the mounting plate is arranged in the counter bore, the circulation hole faces the horn-shaped through hole, and the gland is pressed on the mounting plate from the end face of the outer side of the base flange and is fixed. The sampling valve and the sampling port are detachable, so that the maintenance is convenient; the sampling is the fermentation liquid in the fermentation tank, and the sterilization is thorough and has no dead angle.

Description

Sterile sampling device

Technical Field

The utility model relates to a sampling device of fermentation cylinder, especially an aseptic sampling device.

Background

The microbial fermentation tank generally needs to sample fermentation liquor on time in the culture process, and a laboratory detects some key parameters, such as viable count, glucose concentration and the like, of the sample, so as to analyze the real-time fermentation condition of the microbes. The sampling port of the fermentation tank must be sterilized by steam before sampling, so that the fermentation liquor is prevented from being polluted because the sampling port contains living mixed bacteria in the sampling process. The traditional sampling device generally comprises three small valves, wherein one valve is arranged on a sampling port of the fermentation tank to separate the sampling port from the outside, one valve is connected with a steam pipeline, and the other valve controls the sample to flow out. Install the valve on the fermentation cylinder sample connection because there is a segment pipeline between valve and the fermentation cylinder, can remain fermentation broth before this segment pipeline, can not reflect the true condition of sample fermentation broth, consequently need discharge earlier a part of fermentation broth during the sample and then draw the sample, can cause the waste of fermentation broth like this, especially the flourishing fermentation broth total amount of small-size fermentation cylinder is less, the fermentation broth loss volume is bigger when the final harvest after taking a sample many times in a fermentation period, and sampling device has certain dead angle owing to connecting between the three valve, steam is difficult to flow through the dead angle part and consequently can not thoroughly disinfect, can bring very big microbiological contamination risk for the fermentation culture. In addition, the sampling device is not detachable and is difficult to maintain if damaged.

SUMMERY OF THE UTILITY MODEL

Utility model purpose: to the above problem, the utility model aims at providing an aseptic sampling device, the remaining zymotic fluid does not have, and the loading and unloading of being convenient for is not had the disinfection dead angle.

The technical scheme is as follows: an aseptic sampling device comprises a sampling port and a sampling valve, wherein the sampling port comprises a base flange and a gland, and the sampling valve comprises a mounting plate and a sampling valve body; the base flange is provided with a counter bore from the outer end face of the base flange, a gradually expanded horn-shaped through hole is formed from the bottom surface of the counter bore to the inner end face of the base flange, and the base flange is fixedly installed with the fermentation tank opening from the inner side of the base flange; the inner side end face of the sampling valve body is provided with a circulation hole communicated with the inner cavity, the peripheral wall face of the sampling valve body is provided with two pipelines communicated with the inner cavity, one pipeline is a steam pipeline, the other pipeline is a sample outlet pipeline, valves are respectively arranged on the steam pipeline and the sample outlet pipeline, a movable plunger is arranged in the inner cavity, an annular gap communicated with the steam pipeline and the sample outlet pipeline is arranged between the movable plunger and the inner cavity, and the sampling valve body penetrates through the mounting plate from the inner side of the sampling valve body to be fixed; the mounting plate is arranged in the counter bore, the circulation hole faces the horn-shaped through hole, and the gland is pressed on the mounting plate from the outer end face of the base flange and is fixed.

Further, the gland and the outer end face of the base flange are fastened and fixed through screws.

Furthermore, the gland is of a split structure with two semicircular sheets, so that the structure of the sampling valve can be conveniently adapted to be pressed with the mounting plate and fixed with the base flange.

Furthermore, a sealing ring is arranged between the mounting plate and the bottom surface of the counter bore, so that leakage caused by mounting of the sampling valve and the sampling port is avoided.

Furthermore, the end face of the inner side of the sampling valve body is flush with the end face of the inner side of the mounting plate, and when the fermentation tank is disinfected, the part, communicated with the sampling port, of the sampling valve can be completely disinfected, so that a disinfection dead angle is eliminated.

Further, the diameter of the counter bore is larger than that of the beam opening end of the horn-shaped through hole.

Furthermore, the diameter of the wide end of the horn-shaped through hole is the same as that of the end face of the inner side of the base flange, the base flange is easier to weld with the opening on the wall of the fermentation tank, and the welding forming is better.

Further, the steam pipeline is arranged above the sample outlet pipeline.

Has the advantages that: compared with the prior art, the utility model has the advantages that:

1. the sampling valve can be installed with the sampling port when sampling is needed, so that the sampling valve is convenient to disassemble and assemble and maintain;

2. the port of the sampling valve is basically flush with the wall of the fermentation tank, an additional small section of pipeline is not needed to be connected between the sampling valve and the fermentation tank, a part of fermentation liquor does not need to be discharged in advance during sampling, the extracted sample is fermentation liquor which is uniform and consistent with the fermentation tank, and the waste of the fermentation liquor during sampling is greatly reduced;

3. during sampling and disinfection, the part of the sampling valve, which is in contact with the fermentation liquor on the whole, can be disinfected, so that no disinfection dead angle exists, the disinfection is thorough and complete, and the risk of bacterial contamination caused by sampling can be avoided to the greatest extent;

4. the included angle formed by the wide opening end of the horn-shaped through hole on the sampling port and the wall of the fermentation tank enables the fermentation liquor to automatically flow back into the fermentation equipment and not to remain in the sampling port and the sampling valve.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a cross-sectional view of a base flange;

figure 3 is an end view of the gland.

Detailed Description

The invention will be further elucidated with reference to the drawings and the specific embodiments. These examples are provided only for illustrating the present invention and are not intended to limit the scope of the present invention.

An aseptic sampling device, as shown in figure 1, comprises a sampling port and a sampling valve which are matched and assembled, wherein the sampling port comprises a base flange 1, a gland 2 and a sealing ring 5, and the sampling valve comprises a mounting plate 3 and a sampling valve body 4.

Referring to fig. 2, a counter bore 11 is axially formed in the base flange 1 from the outer end surface thereof, a flared through hole 12 is axially formed in the counter bore 11 from the bottom surface thereof to the inner end surface of the base flange 1, the diameter D1 of the counter bore 11 is greater than the diameter D2 of the end of the flared through hole 12, and the diameter D3 of the wide end of the flared through hole 12 is the same as the diameter D4 of the inner end surface of the base flange 1.

The base flange 1 is welded and fixed with the opening on the wall of the fermentation tank 6 from the outer wall surface of the inner side of the base flange 1, so that the base flange 1 and the fermentation tank 6 are integrated, and the sealing ring 5 is placed on the bottom surface of the counter bore 11.

The sampling valve body 4 is columnar and is provided with an inner cavity 41, the inner side end surface of the columnar body is provided with a circulation hole 42 communicated with the inner cavity 41, the peripheral wall surface of the columnar body is provided with two pipelines communicated with the inner cavity 41, one pipeline is a steam pipeline 43, the other pipeline is a sample outlet pipeline 44, the steam pipeline 43 is arranged above the sample outlet pipeline 44, the steam pipeline 43 and the sample outlet pipeline 44 are respectively provided with a valve 47, a movable plunger 45 is arranged in the inner cavity 41 and can penetrate through the outer side end surface of the columnar body, the movable plunger 45 is sealed with the penetrating part of the columnar body, the movable plunger 45 moves in the inner cavity 41 and can block the circulation hole 42 or ensure that the circulation hole 42 is communicated with the inner cavity 41, the movable plunger 45 is arranged at a section of the steam pipeline 43 and the sample outlet pipeline 44 communicated with the inner cavity 41, the diameter of the section is smaller than that of the inner cavity 41, an annular gap 46 is, the steam pipeline 43 and the sample outlet pipeline 44 are communicated in the inner cavity 41 through an annular gap 46, the columnar body penetrates through a through hole on the mounting plate 3 from the inner side of the columnar body, the outer wall surface of the columnar body is welded and fixed with the through hole, and the end surface of the inner side of the columnar body is flush with the end surface of the inner side of the mounting plate 3.

The sampling valve body 4 is arranged in the counter bore 11 through the mounting plate 3 and is pressed on the sealing ring 5, the circulation hole 42 faces the horn-shaped through hole 12, the gland 2 is pressed on the mounting plate 3 from the outer side end face of the base flange 1, the gland 2 and the outer side end face of the base flange 1 are fastened and fixed through screws, and the mounting of the sampling valve and the sampling port is completed. Because the steam pipeline 43, the sample outlet pipeline 44 and the valve 47 extend out of the cylindrical main body of the sampling valve body 4, as shown in fig. 3, the gland 2 is designed into a split structure of two semicircular plates, which is convenient for final assembly of the gland 2.

After the installation of sample valve and sample connection, during the fermentation cylinder disinfection, the sample valve body is in the closed condition, and the activity plunger removes to the shutoff of convection current through-hole promptly, and all faces that the sample valve was in the horn shape through-hole beam mouth end at this moment disinfect with the fermentation cylinder together. Before the sample, the sample valve body is disinfected, and the sample valve body still is in the closure state, opens the valve on steam line and the play appearance pipeline simultaneously, and steam flows into the hoop clearance between activity plunger and the inner chamber from steam line, flows out from a appearance pipeline again, maintains this operation 20 ~ 30 minutes and thoroughly disinfects the sample valve body. After the sterilization is finished, the valves on the steam pipeline and the sample outlet pipeline are closed, the movable plunger is properly moved out to open the sampling valve body, even if the circulation hole is communicated with the inner cavity, the valve on the sample outlet pipeline is slowly opened, after a proper amount of fermentation liquor sample is taken by using a pre-sterilized reagent bottle, the valves on the sampling valve body and the sample outlet pipeline are closed, and then the sterilization step is repeated to sterilize the sampling valve again.

When the sampling valve is not needed to be installed at the sampling port, the horn-shaped through hole 12 can be plugged by a blind plate matched with the counter bore 11, so that the interior of the fermentation tank is isolated from the outside.

The utility model has the main advantages that:

Claims

1. An aseptic sampling device, characterized by: the sampling valve comprises a sampling port and a sampling valve, wherein the sampling port comprises a base flange (1) and a gland (2), and the sampling valve comprises a mounting plate (3) and a sampling valve body (4); the base flange (1) is provided with a counter bore (11) from the outer end face thereof, a gradually expanding trumpet-shaped through hole (12) is formed from the bottom face of the counter bore (11) to the inner end face of the base flange (1), and the base flange (1) is fixedly installed with the fermentation tank opening from the inner side thereof; the inner side end face of the sampling valve body (4) is provided with a circulation hole (42) communicated with an inner cavity (41), the peripheral wall face of the sampling valve body (4) is provided with two pipelines communicated with the inner cavity (41), one pipeline is a steam pipeline (43), the other pipeline is a sample outlet pipeline (44), the steam pipeline (43) and the sample outlet pipeline (44) are respectively provided with a valve, a movable plunger (45) is arranged in the inner cavity (41), an annular gap (46) communicated with the steam pipeline (43) and the sample outlet pipeline (44) is arranged between the movable plunger (45) and the inner cavity (41), and the sampling valve body (4) is fixedly arranged on the mounting plate (3) in a penetrating mode from the inner side of the sampling valve body (4); the mounting plate (3) is arranged in the counter bore (11), the circulation hole (42) faces the horn-shaped through hole (12), and the gland (2) is pressed on the mounting plate (3) from the outer end face of the base flange (1) and is fixed.

2. The sterile sampling device of claim 1, wherein: the gland (2) and the outer side end face of the base flange (1) are fastened and fixed through screws.

3. The sterile sampling device of claim 1, wherein: the gland (2) is of a split structure with two semicircular plates.

4. The sterile sampling device of claim 1, wherein: and a sealing ring (5) is also arranged between the mounting plate (3) and the bottom surface of the counter bore (11).

5. The sterile sampling device of claim 1, wherein: the end surface of the inner side of the sampling valve body (4) is flush with the end surface of the inner side of the mounting plate (3).

6. The sterile sampling device of claim 1, wherein: the diameter of the counter bore (11) is larger than that of the beam opening end of the horn-shaped through hole (12).

7. The sterile sampling device of claim 1, wherein: the diameter of the wide end of the trumpet-shaped through hole (12) is the same as that of the end surface of the inner side of the base flange (1).

8. The sterile sampling device of claim 1, wherein: the steam pipeline (43) is arranged above the sample outlet pipeline (44).