CN210656926U - Liquid fermentation sterile sampling device - Google Patents
Liquid fermentation sterile sampling device Download PDFInfo
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- CN210656926U CN210656926U CN201921123541.8U CN201921123541U CN210656926U CN 210656926 U CN210656926 U CN 210656926U CN 201921123541 U CN201921123541 U CN 201921123541U CN 210656926 U CN210656926 U CN 210656926U
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Abstract
The utility model discloses a liquid fermentation sterile sampling device, which comprises a sampling mechanism, a conveying mechanism, a sewage draining mechanism and a sample receiving mechanism; the sampling mechanism consists of a fermentation tank sampling port and a quick connector, one end of the fermentation tank sampling port is connected with the fermentation tank, and the other end of the fermentation tank sampling port is connected with one end of the quick connector; the conveying mechanism consists of a fermentation liquid inlet pipe, a three-way valve and a fermentation liquid outlet pipe, wherein one end of the fermentation liquid inlet pipe is connected with the other end of the quick connector, the other end of the fermentation liquid inlet pipe is connected with the three-way valve, and one end of the fermentation liquid outlet pipe is connected with the three-way valve; the blow-down mechanism consists of a blow-down pipe and a blow-down valve, one end of the blow-down pipe is connected with the three-way valve, and the blow-down valve is arranged at the outlet of the blow-down pipe; the sample receiving mechanism consists of a plastic joint and a sampler, one end of the plastic joint is arranged at the outlet of the other end of the fermentation liquid outlet pipe, and the sampler is connected with the other end of the plastic joint. The fermentation liquid can be quickly, simply and safely taken out from the fermentation tank by adopting the design.
Description
Technical Field
The utility model relates to a biological fermentation equipment technical field, in particular to aseptic sampling device of liquid fermentation.
Background
Fermentation refers to the process by which a person produces the microbial cells themselves, or direct or secondary metabolites, by virtue of the life activities of the microorganism under aerobic or anaerobic conditions. Fermentation is sometimes also referred to as fermentation filtration, the definition of which varies depending on the application. Generally, fermentation refers to a process of decomposing organic substances by organisms. Fermentation is a biochemical reaction that humans have come into contact with earlier and is now widely used in the food industry, the biological and chemical industries. It is also a basic process of bioengineering, i.e. fermentation engineering.
The fermentation liquid is required to be carried out in a sterile environment in the process of fermentation in a fermentation tank, so that the fermentation liquid is prevented from being polluted by other mixed bacteria. When the condition of fermentation liquor needs to be detected in the fermentation process, a sterile liquor taking device is needed for sampling; the existing common fermentation liquor sampling methods are generally an up-sampling method and a down-sampling method, and sampling is carried out through a sampling valve; although the existing liquid fermentation sampling valve can ensure that the fermentation liquor can be smoothly taken out from the fermentation tank, and the pollution to the fermentation liquor in the tank can be avoided. However, it cannot be guaranteed that the taken fermentation liquid is not polluted, and the fermentation liquid taken out of the fermentation tank by a common sampling valve is easily influenced by the surrounding environment and is polluted by microorganisms, so that the fermentation liquid measurement has larger errors.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to provide an aseptic sampling device of liquid fermentation can dock with the fermentation cylinder on the market to guarantee that the zymotic fluid of taking out can not receive the pollution of microorganism in the environment.
In order to solve the technical problem, the utility model discloses a technical scheme does:
a liquid fermentation sterile sampling device comprises a sampling mechanism, a conveying mechanism, a pollution discharge mechanism and a sample receiving mechanism; the sampling mechanism consists of a fermentation tank sampling port and a quick connector, one end of the fermentation tank sampling port is hermetically connected with the fermentation tank, and the other end of the fermentation tank sampling port is connected with one end of the quick connector;
the conveying mechanism consists of a fermentation liquid inlet pipe, a three-way valve and a fermentation liquid outlet pipe, one end of the fermentation liquid inlet pipe is connected with the other end of the quick connector, the other end of the fermentation liquid inlet pipe is connected with the three-way valve, and one end of the fermentation liquid outlet pipe is connected with the three-way valve;
the blowdown mechanism consists of a blowdown pipe and a blowdown valve, one end of the blowdown pipe is connected with the three-way valve, and the blowdown valve is arranged at the outlet of the blowdown pipe;
the sample receiving mechanism consists of a plastic joint and a sampler, one end of the plastic joint is arranged at the outlet of the other end of the fermentation liquid outlet pipe, and the sampler is connected to the other end of the plastic joint; threads are arranged at one end of the plastic joint and the opening of the sampler and are matched with the threads at one end of the plastic joint and the opening of the sampler.
As a preferred embodiment, the fermentation liquid inlet pipe, the fermentation liquid outlet pipe and the sewage discharge pipe are all made of medical stainless steel.
In a preferred embodiment, the inner diameter of the quick connector is matched with the outer diameter of the sampling port of the fermentation tank.
In a preferred embodiment, one end of the plastic joint and the mouth of the sampler are both provided with threads, and the threads at one end of the plastic joint are matched with the threads at the mouth of the sampler.
As a preferred embodiment, the sampler is a sampling bottle having a volume of not more than 100 ml.
The utility model adopts the technical proposal, the conveying mechanism, the sewage draining mechanism and the sample receiving mechanism are adjusted by the three-way valve to take samples; the sampling device has simple structure and low manufacturing cost, can be butted with most fermentation tanks on the market, and can quickly and safely take out samples which are not polluted by external microorganisms from the fermentation tanks.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive exercise.
FIG. 1 is a schematic structural view of the liquid fermentation sterile sampling device of the present invention;
in the figure, 1-a sampler, 2-a plastic joint, 3-a fermentation liquor outlet pipe, 4-a three-way valve, 5-a fermentation liquor inlet pipe, 6-a quick joint, 7-a blow-off pipe, 8-a blow-off valve and 9-a fermentation tank sampling port.
Detailed Description
The following describes the present invention with reference to the accompanying drawings. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features related to the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
As shown in figure 1, the liquid fermentation sterile sampling device comprises a sampling mechanism, a conveying mechanism, a sewage discharging mechanism and a sample receiving mechanism; the sampling mechanism consists of a fermentation tank sampling port 9 and a quick connector 6, wherein one end of the fermentation tank sampling port 9 is hermetically connected with a fermentation tank, and the other end of the fermentation tank sampling port 9 is connected with one end of the quick connector 6; wherein, the inner diameter of the quick connector 6 is matched with the outer diameter of the sampling port 9 of the fermentation tank. The conveying mechanism is composed of a fermentation liquid inlet pipe 5, a three-way valve 4 and a fermentation liquid outlet pipe 3, one end of the fermentation liquid inlet pipe 5 is connected with the other end of the quick connector 6, the other end of the fermentation liquid inlet pipe 5 is connected with the three-way valve 4, and one end of the fermentation liquid outlet pipe 3 is connected with the three-way valve 4. The blowdown mechanism consists of a blowdown pipe 7 and a blowdown valve 8, one end of the blowdown pipe 7 is connected with the three-way valve 4, and the blowdown valve 8 is arranged at the outlet of the blowdown pipe 7; the sample receiving mechanism consists of a plastic joint 2 and a sampler 1, wherein one end of the plastic joint 2 is arranged at the outlet of the other end of the fermentation liquid outlet pipe 3, and the sampler 1 is connected to the other end of the plastic joint 2.
As a preferred implementation mode, the fermentation liquid inlet pipe 5, the fermentation liquid outlet pipe 3 and the sewage discharge pipe 7 are all made of medical stainless steel; the sampler 1 is a sampling bottle with a volume not exceeding 100 ml.
Specifically, the complete working principle of the device is as follows:
(1) the quick connector 6 is bound by 4 layers of gauze and kraft paper, the blowoff valve 8 is closed, the three-way valve 4 is adjusted to ensure that the fermentation liquor outlet pipe 3 and the fermentation liquor inlet pipe 5 are communicated, the sampler 1 and the plastic connector 2 are tightly connected through matched threads, and then the components 1-8 are integrally placed in a high-pressure steam sterilization cabinet to be sterilized for 30 minutes under the condition of high-pressure steam at 121 ℃. After the sterilization is finished, the fermentation liquid inlet pipe 5 is communicated with the sewage discharge pipe 7 by adjusting the three-way valve 4.
(2) The quick connector 6 is in butt joint with the sampling port 9 of the fermentation tank, steam is introduced through the sampling port 9 of the fermentation tank, the blowoff valve 8 is opened to enable the steam to flow out, and the sampling port 9 of the fermentation tank is disinfected for 30 minutes by utilizing the flowing steam.
(3) Stopping leading steam, closing the blow-down valve 8, making the fermentation liquor flow into the fermentation liquor inlet pipe 5 and the blow-down pipe 7 through the sampling port 9 of the fermentation tank, discharging part of the fermentation liquor through the blow-down valve 8, and discharging redundant steam in the fermentation liquor inlet pipe 5 and the blow-down pipe 7.
(4) And closing the blowoff valve 8, adjusting the three-way valve 4 to ensure that the fermentation liquid outlet pipe 3 is communicated with the fermentation liquid inlet pipe 5, so that the fermentation liquid enters the fermentation liquid inlet pipe 5 from the fermentation tank through the sampling port 9 and the quick connector 6, enters the fermentation liquid outlet pipe 3 through the limitation of the three-way valve 4, and finally flows into the sampler 1.
(5) Closing the fermentation sampling valve, adjusting the three-way valve 4 to communicate the fermentation liquid inlet pipe 5 with the drain pipe 7, closing the communication between the fermentation liquid inlet pipe 5 and the drain pipe 7 with the fermentation liquid outlet pipe 3, introducing steam for 2 minutes, blowing away residual fermentation liquid in the fermentation liquid inlet pipe 5 and the drain pipe 7 by utilizing flowing steam, and opening the drain valve 8 to discharge the residual fermentation liquid.
(6) The quick connector 6 is detached, and the whole liquid fermentation sterile sampling device is transferred to a biological safety cabinet for operation.
The utility model discloses an above-mentioned design, can dock with the fermentation cylinder on the market to the zymotic fluid of guaranteeing to take out can not receive the pollution of microorganism in the environment. Through the above-mentioned operation process, the extracted fermentation liquid is usually used for subsequent cultivation, such as plating a plate, or inoculating a liquid culture medium, so as to accurately analyze whether the batch of fermentation is contaminated with infectious microbes.
The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in the embodiments without departing from the principles and spirit of the invention, and the scope of the invention is to be accorded the full scope of the claims.
Claims (5)
1. The utility model provides a sterile sampling device of liquid fermentation which characterized in that: comprises a sampling mechanism, a conveying mechanism, a pollution discharge mechanism and a sample receiving mechanism; the sampling mechanism consists of a fermentation tank sampling port (9) and a quick connector (6), one end of the fermentation tank sampling port (9) is hermetically connected with the fermentation tank, and the other end of the fermentation tank sampling port (9) is connected with one end of the quick connector (6);
the conveying mechanism consists of a fermentation liquid inlet pipe (5), a three-way valve (4) and a fermentation liquid outlet pipe (3), one end of the fermentation liquid inlet pipe (5) is connected with the other end of the quick connector (6), the other end of the fermentation liquid inlet pipe (5) is connected with the three-way valve (4), and one end of the fermentation liquid outlet pipe (3) is connected with the three-way valve (4);
the sewage draining mechanism consists of a sewage draining pipe (7) and a sewage draining valve (8), one end of the sewage draining pipe (7) is connected with the three-way valve (4), and the sewage draining valve (8) is arranged at the outlet of the sewage draining pipe (7);
connect appearance mechanism to constitute by plastic joint (2) and sampler (1), the one end of plastic joint (2) is installed the other end exit of zymotic fluid drain pipe (3), sampler (1) is connected the other end of plastic joint (2).
2. The liquid fermentation sterile sampling device of claim 1, wherein: the fermentation liquid inlet pipe (5), the fermentation liquid outlet pipe (3) and the blow-off pipe (7) are all made of medical stainless steel.
3. The liquid fermentation sterile sampling device of claim 1, wherein: the inner diameter of the quick connector (6) is matched with the outer diameter of the sampling port (9) of the fermentation tank.
4. The liquid fermentation sterile sampling device of claim 1, wherein: threads are arranged at one end of the plastic joint (2) and the opening of the sampler (1) and are matched with the threads at one end of the plastic joint (2) and the opening of the sampler (1).
5. The liquid fermentation sterile sampling device of claim 1, wherein: the sampler (1) is a sampling bottle with the volume not exceeding 100 ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921123541.8U CN210656926U (en) | 2019-07-17 | 2019-07-17 | Liquid fermentation sterile sampling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921123541.8U CN210656926U (en) | 2019-07-17 | 2019-07-17 | Liquid fermentation sterile sampling device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210656926U true CN210656926U (en) | 2020-06-02 |
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ID=70838735
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921123541.8U Active CN210656926U (en) | 2019-07-17 | 2019-07-17 | Liquid fermentation sterile sampling device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210656926U (en) |
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2019
- 2019-07-17 CN CN201921123541.8U patent/CN210656926U/en active Active
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