CN219279884U

CN219279884U - Bioreactor inoculation system

Info

Publication number: CN219279884U
Application number: CN202320090902.3U
Authority: CN
Inventors: 梁贤; 刘晓倩; 杨建军; 胡晓明; 张喜美
Original assignee: Shandong Delinore Bio Engineering Co ltd
Current assignee: Shandong Delinore Bio Engineering Co ltd
Priority date: 2023-01-31
Filing date: 2023-01-31
Publication date: 2023-06-30
Anticipated expiration: 2033-01-31

Abstract

The utility model relates to the technical field of inoculation equipment, in particular to a bioreactor inoculation system, which comprises: the device comprises a reactor tank body, a pump, a first valve, a second valve, a third valve, a fourth valve, a fifth valve and a seed bottle; the reactor tank body, the pump, the first valve, the second valve, the third valve and the seed bottle are sequentially connected through pipelines; a steam inlet pipeline is arranged on the pipeline between the first valve and the second valve, and a fourth valve is arranged on the steam inlet pipeline; a steam outlet pipeline is arranged on the pipeline between the second valve and the third valve, and a fifth valve is arranged on the steam outlet pipeline; according to the utility model, through the matching arrangement of the valves, the steam sterilization of the inoculation pipeline can be realized, and the steam cleaning can be realized after the inoculation is completed; each valve is a quick-connection valve, is convenient to disassemble and use, and can inoculate a plurality of bioreactors; by using the peristaltic pump, the pump is prevented from polluting inoculation liquid, the tightness is good, and the backflow of the inoculation liquid is avoided.

Description

Bioreactor inoculation system

Technical Field

The utility model relates to the technical field of inoculation equipment, in particular to a bioreactor inoculation system.

Background

The bioreactor is a core device for providing a good reaction environment for microbial fermentation or cell culture to complete biocatalysis reaction and bears the production task of the product. Its structure, operation mode and operation condition have close relation to quality, conversion rate and energy consumption of biological process product. In the processes of cell culture and virus propagation production, the inoculation operation of the culture system is the operation step with the greatest risk of pollution, and the whole culture system is lost due to deviation in the inoculation process.

The traditional liquid inoculation mode is that a sealing cover plate of an inoculation port of a reactor tank body is taken down, and prepared cells or virus liquid is transferred into the reactor under the protection of flame. The operation flow has the problems that a plurality of persons are required to cooperatively operate, potential safety hazards exist when open fire is used, system pollution is easy to cause in an opening process and the like, and therefore, the bioreactor inoculation system is provided.

Disclosure of Invention

The utility model aims to provide a bioreactor inoculation system, which solves the problems that a plurality of persons are required to cooperatively operate, potential safety hazards exist and the opening process is easy to cause system pollution in the background technology.

In order to solve the technical problems, the utility model provides the following technical scheme:

a bioreactor inoculation system comprising: the device comprises a reactor tank body, a pump, a first valve, a second valve, a third valve, a fourth valve, a fifth valve and a seed bottle;

the reactor tank body, the pump, the first valve, the second valve, the third valve and the seed bottle are sequentially connected through pipelines;

a steam inlet pipeline is arranged on a pipeline between the first valve and the second valve, and a fourth valve is arranged on the steam inlet pipeline;

and a steam outlet pipeline is arranged on the pipeline between the second valve and the third valve, and a fifth valve is arranged on the steam outlet pipeline.

Further, the first valve, the second valve, the third valve, the fourth valve and the fifth valve are quick-connection valves.

The first valve, the second valve, the third valve, the fourth valve and the fifth valve can be quickly disassembled and assembled, and the off-line sterilization is performed after the disassembly, so that the sterilization effect is enhanced.

Further, the seed bottle is provided with a liquid pipe and an air pipe, and the liquid pipe and the air pipe are both used for communicating the inside and the outside of the seed bottle; the liquid pipe is connected with the third valve through a pipeline and extends to the bottom of the seed bottle; the air tube extends to an upper portion of the seed bottle.

And pumping the inoculation liquid in the seed bottle into the reactor tank body through the pumping action of the pump, wherein the air pipe is used for balancing the internal and external pressure of the seed bottle.

Further, one end of the air pipe, which is far away from the seed bottle, is provided with an air filter.

The air filter is used for filtering the mixed bacteria in the air, so as to avoid pollution to inoculation liquid.

Further, the pipeline between the reactor tank body and the first valve is a silica gel hose.

Further, the pump is a peristaltic pump.

The peristaltic pump is used, and the inoculating liquid only contacts the pump pipe and does not contact the pump body; the peristaltic pump has good tightness and self-absorption capacity, and can prevent the backflow of inoculation liquid.

The beneficial effects of the utility model are as follows:

according to the utility model, through the matching arrangement of the valves, the steam sterilization of the inoculation pipeline can be realized, and the steam cleaning can be realized after the inoculation is completed; each valve is a quick-connection valve, is convenient to disassemble and use, and can inoculate a plurality of bioreactors; by using the peristaltic pump, the pump is prevented from polluting inoculation liquid, the tightness is good, and the backflow of the inoculation liquid is avoided.

Drawings

The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the utility model and do not constitute a limitation on the utility model. In the drawings:

FIG. 1 is a schematic diagram of a bioreactor inoculation system according to the present utility model;

in the figure: 1. a reactor tank; 2. a peristaltic pump; 3. a first valve; 4. a second valve; 5. a third valve; 6. a fourth valve; 7. a fifth valve; 8. seed bottles; 9. a steam inlet pipeline; 10. a steam outlet pipeline; 11. a silicone hose; 12. an air filter.

Detailed Description

In order to more clearly illustrate the general concepts of the present application, a detailed description is provided below by way of example in connection with the accompanying drawings.

In one embodiment, as shown in fig. 1, a bioreactor inoculation system, comprising: the reactor comprises a reactor tank 1, a pump, a first valve 3, a second valve 4, a third valve 5, a fourth valve 6, a fifth valve 7 and a seed bottle 8;

the reactor tank 1, the pump, the first valve 3, the second valve 4, the third valve 5 and the seed bottle 8 are connected in sequence through pipelines;

a steam inlet pipeline 9 is arranged on a pipeline between the first valve 3 and the second valve 4, and a fourth valve 6 is arranged on the steam inlet pipeline 9;

a steam outlet pipeline 10 is arranged on a pipeline between the second valve 4 and the third valve 5, and a fifth valve 7 is arranged on the steam outlet pipeline 10.

It can be understood that the first valve 3, the second valve 4, the third valve 5, the fourth valve 6 and the fifth valve 7 are quick-connection valves.

The first valve 3, the second valve 4, the third valve 5, the fourth valve 6 and the fifth valve 7 can be quickly disassembled and assembled, and the off-line sterilization is performed after the disassembly, so that the sterilization effect is enhanced.

It will be appreciated that the first valve 3, the second valve 4, the third valve 5, the fourth valve 6, the fifth valve 7 and the seed bottle 8 are disassembled for off-line sterilization before use. Before the reactor tank 1 is empty, the pipelines where the first valve 3, the second valve 4 and the fourth valve 6 are positioned are arranged to the reactor tank 1, the sterilization treatment is carried out while the reactor tank 1 is empty, and the valves are closed after the sterilization is finished, so that pollution is prevented.

Before inoculation, a third valve 5, a fifth valve 7 and a seed bottle 8 are connected, pure steam is introduced into a steam inlet pipeline 9, a fourth valve 6, a second valve 4 and a fifth valve 7 are opened, steam is discharged from a steam outlet pipeline 10, and a pipeline installed at the back is sterilized, so that the system is ensured to be in a sterile state. After the pipeline is thoroughly sterilized, the fourth valve 6 and the fifth valve 7 are closed, and the first valve 3, the second valve 4 and the third valve 5 are opened.

During inoculation, the pump is started to transfer the inoculation liquid into the reactor tank 1, and the inoculation operation is completed.

After inoculation is completed, pure steam is introduced into the steam inlet pipeline 9 again, the first valve 3 is closed, the fourth valve 6, the second valve 4, the third valve 5 and the fifth valve 7 are opened to sterilize the used pipeline and the seed bottle 8, and related components are removed to complete production operation.

In another embodiment, as shown in fig. 1, the seed bottle 8 is provided with a liquid pipe and an air pipe, and the liquid pipe and the air pipe are used for communicating the inside and the outside of the seed bottle 8; the liquid pipe is connected with the third valve 5 through a pipeline and extends to the bottom of the seed bottle 8; the air tube extends to the upper part of the seed bottle 8.

The inoculating liquid in the seed bottle 8 is pumped into the reactor tank 1 by the pumping action of the pump, and the air pipe is used for balancing the internal and external pressure of the seed bottle 8.

It will be appreciated that the end of the air tube remote from the seed bottle 8 is provided with an air filter 12.

The air filter 12 is used to filter the air of the bacteria and to avoid contamination of the inoculation liquid.

It will be appreciated that the conduit between the reactor tank 1 and the first valve 3 is a silicone hose 11.

It will be appreciated that the pump is a peristaltic pump 2.

Using peristaltic pump 2, inoculating liquid only contacts pump tube, not contact pump body; the peristaltic pump 2 has good tightness and self-priming capability, and can prevent the backflow of inoculation liquid.

It can be understood that in the use process, the reactor tank 1 is connected with the first valve 3 through the silica gel hose 11, and the peristaltic pump 2 is matched for use, so that the inoculation liquid is conveyed into the reactor tank 1, and the peristaltic pump 2 has high repetition precision and high stability precision, and can not make the inoculation liquid contact with the external air; by using the air filter 12, contamination of the inoculation liquid with foreign bacteria in the air is also avoided.

In this specification, each embodiment is described in a progressive manner, and identical and similar parts of each embodiment are all referred to each other, and each embodiment mainly describes differences from other embodiments. In particular, for system embodiments, since they are substantially similar to method embodiments, the description is relatively simple, as relevant to see a section of the description of method embodiments.

The foregoing is merely exemplary of the present utility model and is not intended to limit the present utility model. Various modifications and variations of the present utility model will be apparent to those skilled in the art. Any modification, equivalent replacement, improvement, etc. which come within the spirit and principles of the utility model are to be included in the scope of the claims of the present utility model.

Claims

1. A bioreactor inoculation system, comprising: the reactor comprises a reactor tank body (1), a pump, a first valve (3), a second valve (4), a third valve (5), a fourth valve (6), a fifth valve (7) and a seed bottle (8);

the reactor tank body (1), the pump, the first valve (3), the second valve (4), the third valve (5) and the seed bottle (8) are sequentially connected through pipelines;

a steam inlet pipeline (9) is arranged on a pipeline between the first valve (3) and the second valve (4), and a fourth valve (6) is arranged on the steam inlet pipeline (9);

a steam outlet pipeline (10) is arranged on a pipeline between the second valve (4) and the third valve (5), and a fifth valve (7) is arranged on the steam outlet pipeline (10).

2. Bioreactor inoculation system according to claim 1, characterized in that the first valve (3), the second valve (4), the third valve (5), the fourth valve (6), the fifth valve (7) are quick-connect valves.

3. Bioreactor inoculation system according to claim 1, characterized in that said seed bottle (8) is provided with a liquid tube and an air tube, both for communicating the inside and the outside of said seed bottle (8); the liquid pipe is connected with the third valve (5) through a pipeline and extends to the bottom of the seed bottle (8); the air tube extends to the upper part of the seed bottle (8).

4. A bioreactor inoculation system as claimed in claim 3, characterized in that the end of the air tube remote from the seed bottle (8) is provided with an air filter (12).

5. Bioreactor inoculation system according to claim 1, characterized in that the conduit between the reactor tank (1) and the first valve (3) is a silicone hose (11).

6. Bioreactor inoculation system according to claim 5, characterized in that said pump is a peristaltic pump (2).