CN209967124U - Culture medium sterility maintaining system - Google Patents
Culture medium sterility maintaining system Download PDFInfo
- Publication number
- CN209967124U CN209967124U CN201920212001.0U CN201920212001U CN209967124U CN 209967124 U CN209967124 U CN 209967124U CN 201920212001 U CN201920212001 U CN 201920212001U CN 209967124 U CN209967124 U CN 209967124U
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- China
- Prior art keywords
- tank body
- inner tank
- air
- culture medium
- culture
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- Expired - Fee Related
Links
- 239000001963 growth medium Substances 0.000 title claims abstract description 30
- 230000036512 infertility Effects 0.000 title claims description 12
- 230000001954 sterilising effect Effects 0.000 claims abstract description 18
- 238000007789 sealing Methods 0.000 claims abstract description 16
- 238000010438 heat treatment Methods 0.000 claims abstract description 13
- 238000011177 media preparation Methods 0.000 claims abstract description 13
- 238000001914 filtration Methods 0.000 claims abstract description 11
- 238000003860 storage Methods 0.000 claims abstract description 11
- 230000005540 biological transmission Effects 0.000 claims abstract description 9
- 238000011049 filling Methods 0.000 claims abstract description 9
- 238000012423 maintenance Methods 0.000 claims abstract description 6
- 238000001514 detection method Methods 0.000 claims abstract description 4
- 238000002955 isolation Methods 0.000 claims description 17
- 238000003756 stirring Methods 0.000 claims description 15
- 238000004891 communication Methods 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 230000002045 lasting effect Effects 0.000 abstract 1
- 239000007789 gas Substances 0.000 description 7
- 238000004659 sterilization and disinfection Methods 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 229910001220 stainless steel Inorganic materials 0.000 description 6
- 239000010935 stainless steel Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- 239000003638 chemical reducing agent Substances 0.000 description 3
- 238000007599 discharging Methods 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000009172 bursting Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000002070 germicidal effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a culture medium aseptic maintenance system, a serial communication port, include: a culture medium preparation tank, a filtering device, an ultrasonic sterilizing device, a filling device and an aseptic storage device for storing culture dishes filled with culture solution, which are sequentially connected through a transmission pipe; the culture medium preparation tank comprises an inner tank body for containing culture solution, an outer tank body arranged on the outer side of the inner tank body, and a sealing cover for sealing the inner tank body; the sealing cover is provided with a discharge channel, a pressure detection device and a high-pressure gas inlet and outlet channel; the heating device comprises an outer tank body and an inner tank body, wherein a heating interval is reserved between the outer tank body and the inner tank body, an air passage through which high-temperature air can pass is arranged in the heating interval, the air passage is attached to the outer side wall of the inner tank body, and an air inlet and an air outlet of the air passage penetrate out of the outer tank body and are communicated with the outside. The utility model discloses an above-mentioned structure is effectual has realized the lasting sterile condition of culture medium, has promoted the high-efficient utilization of production data.
Description
Technical Field
The utility model relates to a culture medium sterility maintaining system.
Background
Culture medium is cultivated to a development direction that current medicinal material was planted, but at the in-process that the medicinal material was planted, because the filling of culture medium and the absolute isolation that can't accomplish with the external world in the save process lead to the culture medium to be contaminated by microorganism, bacterium easily to the production data is by extravagant condition appears, simultaneously at the configuration in-process of culture medium, current culture medium configuration device also often can also because to the uneven problem that the sterilizing effect is poor appears of culture solution heating.
Disclosure of Invention
In order to solve the above technical problems, the present invention provides a culture medium sterility maintaining system, which can greatly reduce the probability of contamination of culture medium during the configuration, filling and storage processes.
A media sterility maintenance system, comprising:
a culture medium preparation tank, a filtering device, an ultrasonic sterilizing device, a filling device and an aseptic storage device for storing culture dishes filled with culture solution, which are sequentially connected through a transmission pipe;
the culture medium preparation tank comprises an inner tank body for containing culture solution, an outer tank body arranged on the outer side of the inner tank body, and a sealing cover for sealing the inner tank body;
the sealing cover is provided with a discharge channel, a pressure detection device and a high-pressure gas inlet and outlet channel;
the heating device comprises an outer tank body and an inner tank body, wherein a heating interval is reserved between the outer tank body and the inner tank body, an air passage through which high-temperature air can pass is arranged in the heating interval, the air passage is attached to the outer side wall of the inner tank body, and an air inlet and an air outlet of the air passage penetrate out of the outer tank body and are communicated with the outside.
Further, the air flue is of a double-spiral structure which bypasses from the upper part of the inner tank body to the lower part of the inner tank body, and the air inlet and the air outlet are both positioned on the upper part.
Further, a stirring device is arranged in the inner tank body.
Further, agitating unit is two stirring rake structures, the paddle of two stirring rakes is opposite with the inclination of horizontal plane.
Further, aseptic storage device includes the conveying platform, conveying platform upper portion is provided with the isolation chamber that keeps apart culture dish on the conveying platform with external environment, the isolation chamber top is provided with new trend device.
Furthermore, an ultraviolet sterilizing lamp is arranged at the top of the isolation chamber and surrounds an air outlet of the fresh air device.
The utility model has the advantages that:
1. the utility model discloses an air flue in the culture medium preparation jar has realized preparing the even heating of jar interior culture solution to the culture medium to high temperature sterilization's application range and efficiency have been promoted.
2. The double helix structure further improves the uniformity of the high-temperature gas for heating the culture solution in the inner tank body, and further improves the high-temperature extinction effect.
3. Agitating unit can fully stir the culture solution in the inner tank body, makes to have abundant energy transmission between the culture solution inside and outside to make the culture solution can be more even by the heating, thereby promoted the sterilization effect of high temperature to the culture solution.
4. The problem that the stirring effect is reduced because the culture solution is changed from the initial turbulent flow state to the laminar flow state after long-time stirring in the common unidirectional stirring can be solved by the double-stirring-paddle reverse paddle inclined surfaces.
5. The fresh air system can enable the isolation room to be in a positive pressure state relative to the external space all the time, so that the problem that external pollutants enter the isolation room through air to pollute a culture medium is avoided.
6. The ultraviolet sterilization lamp can further improve the sterility keeping time of the isolation chamber.
Drawings
Fig. 1 is a schematic structural diagram of the present invention;
fig. 2 is a schematic structural diagram of the air passage of the present invention.
Reference numerals: 100. the culture medium preparation tank 110, the inner tank body 120, the outer tank body 130, the sealing cover 131, the discharge channel 132, the high-pressure air inlet and outlet channel 132a, the first end port 132b, the second end port 132c, the third end port 133, the pressure detection device 134, the third control valve 135, the fourth control valve 136, the fifth control valve 140, the temperature rise interval 150, the air channel 151, the air inlet 152, the air outlet 200, the filter device 300, the ultrasonic sterilization device 400, the filling device 500, the sterile storage device 520, the isolation chamber 530, the fresh air device 540, the ultraviolet sterilization lamp 600, the transmission pipe 610, the first control valve 620, the second control valve 700, the stirring device 710, the motor reducer 720, the stirring paddle
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
A culture medium sterility maintaining system as shown in fig. 1 includes a culture medium preparation tank 100, a filtering device 200, an ultrasonic sterilizing device 300, a filling device 400, and a sterility storage device 500 for storing culture dishes filled with a culture solution.
Wherein, the culture medium preparation tank is communicated with the filtering device through a transmission pipe, and a first control valve 610 for controlling the communication between the culture medium preparation tank and the filtering device is also arranged on the transmission pipe 600 between the culture medium preparation tank and the filtering device, and the control valve is a butterfly valve.
The filtering device 200 and the ultrasonic sterilizing device 300 are also communicated with each other through a transmission pipe 600, and a second control valve 620 which is also a butterfly valve is arranged on the transmission pipe between the filtering device and the ultrasonic sterilizing device and controls whether the filtering device and the ultrasonic sterilizing device are communicated with each other or not.
Similarly, the ultrasonic sterilization device 300 and the filling device 400 are also in tank communication via a transfer tube 600.
And the filling device 400 is mounted in the aseptic storage device 500.
In detail, in this embodiment, the medium preparation tank 100 includes an inner tank 110 made of stainless steel and an outer tank 120 made of stainless steel and disposed outside the inner tank; meanwhile, a sealing cover 130 for isolating the inner tank from the outside is provided at the upper part of the inner tank, the sealing cover is also made of stainless steel, a sealing ring (not shown) for improving the sealing effect is provided in the sealing cover, and a discharging passage 131, a high-pressure gas inlet/outlet passage 132 and a pressure detecting device 133 are provided at the upper part of the sealing cover; in detail, the discharging channel 131 is made of stainless steel and is connected to an external culture solution storage tank, and a third control valve 134, which is a butterfly valve in this embodiment, is further provided on the discharging device. The high-pressure gas inlet/outlet channel 132 is also made of stainless steel, and is a three-way pipe, a first end port 132a of the high-pressure gas inlet/outlet channel is communicated with the inside of the inner tank, a second end port 132b of the high-pressure gas inlet/outlet channel is communicated with the nitrogen tank, and a third end port 132c of the high-pressure gas inlet/outlet channel is communicated with the external environment. In this embodiment, a fourth control valve 135 is provided near the second end port, and a fifth control valve 136 is provided near the third end port.
When the culture medium is needed in the inner tank, the worker opens the third control valve 134 and puts the non-sterilized culture medium into the inner tank. When the culture solution in the inner tank body after high-temperature sterilization needs to be removed, a worker opens the fourth control valve 135, then high-pressure nitrogen in the nitrogen tank enters the inner tank body 110 after being filtered and sterilized by the air filtering membrane, and the pressure in the inner tank body is increased, so that the culture solution in the inner tank body is pressed out of the inner tank body. When the pressure detecting device 133 detects that the pressure in the inner tank is too high due to high temperature heating, the operator opens the fifth control valve 136 to release a part of the pressure in the inner tank, so as to protect the inner pipe from bursting due to too high internal pressure.
As shown in FIG. 1, a warming interval 140 is further provided between the inner vessel 110 and the outer vessel 120, and an air passage 150 for allowing external high temperature air to enter therethrough to heat the culture solution in the inner vessel is formed around the outer wall of the inner vessel. The air channel is a copper pipe or an aluminum pipe. And the air inlet 151 and the air outlet 152 of the air duct 150 communicate with the outside through the outer tank.
On the basis of this embodiment, in order to heat the culture solution in the inner tank more uniformly, as shown in fig. 2, the air passage has a double spiral structure, and specifically, the air passage is folded in half from the upper portion of the inner tank to the lower portion of the inner tank.
On the basis of this embodiment, in order to make the culture solution in the inner tank body be heated more evenly fast, be provided with a agitating unit 700 in inner tank body bottom, this agitating unit includes a motor reducer 710 to and link to each other with motor reducer's output shaft, and stretch into the stirring rake 720 to inner tank body inside. Wherein, in order to promote the stirring effect, the quantity of stirring rake is two, and the inclination with the horizontal plane between the paddle of these two stirring rakes is opposite simultaneously.
As shown in fig. 1, the sterile storage device 500 comprises a transfer platform 510 for transferring the culture dishes filled with the culture medium by a conveyor belt, and an isolation chamber 520 for isolating the culture dishes on the transfer platform from the external environment is arranged above the transfer platform, and a fresh air device is arranged at the top of the isolation chamber. In detail, the isolation chamber is a stainless steel frame and is constructed by a transparent acrylic plate. And the fresh air device is provided with a sterilizing device to ensure that no pollution source exists in the air entering the isolation room.
On the basis of this embodiment, for the aseptic environment in the further promotion isolation chamber, still be provided with the ultraviolet germicidal lamp that the round set up around new trend device edge at the top of isolation chamber.
Claims (4)
1. A media sterility maintenance system, comprising:
a culture medium preparation tank, a filtering device, an ultrasonic sterilizing device, a filling device and an aseptic storage device for storing culture dishes filled with culture solution, which are sequentially connected through a transmission pipe;
the culture medium preparation tank comprises an inner tank body for containing culture solution, an outer tank body arranged on the outer side of the inner tank body, and a sealing cover for sealing the inner tank body;
the sealing cover is provided with a discharge channel, a pressure detection device and a high-pressure gas inlet and outlet channel;
a heating interval is reserved between the outer tank body and the inner tank body, an air passage through which high-temperature air can pass is arranged in the heating interval, the air passage is attached to the outer side wall of the inner tank body, and an air inlet and an air outlet of the air passage penetrate through the outer tank body to be communicated with the outside;
the internal agitating unit that is provided with of inner tank, agitating unit is two stirring rake structures, the paddle of two stirring rakes is opposite with the inclination of horizontal plane.
2. A media sterility maintenance system as in claim 1, wherein: the air flue is a double-spiral structure which bypasses from the upper part of the inner tank body to the lower part of the inner tank body, and the air inlet and the air outlet are both positioned at the upper part.
3. A media sterility maintenance system as in claim 1, wherein: the sterile storage device comprises a conveying platform, an isolation chamber for isolating a culture dish on the conveying platform from the external environment is arranged on the upper portion of the conveying platform, and a fresh air device is arranged at the top of the isolation chamber.
4. A media sterility maintenance system according to claim 3, wherein: the top of the isolation chamber is provided with an ultraviolet sterilizing lamp which is arranged around an air outlet of the fresh air device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920212001.0U CN209967124U (en) | 2019-02-18 | 2019-02-18 | Culture medium sterility maintaining system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920212001.0U CN209967124U (en) | 2019-02-18 | 2019-02-18 | Culture medium sterility maintaining system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209967124U true CN209967124U (en) | 2020-01-21 |
Family
ID=69253334
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920212001.0U Expired - Fee Related CN209967124U (en) | 2019-02-18 | 2019-02-18 | Culture medium sterility maintaining system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209967124U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111500434A (en) * | 2020-04-30 | 2020-08-07 | 樟树市狮王生物科技有限公司 | Polyglutamic acid production and processing equipment |
-
2019
- 2019-02-18 CN CN201920212001.0U patent/CN209967124U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111500434A (en) * | 2020-04-30 | 2020-08-07 | 樟树市狮王生物科技有限公司 | Polyglutamic acid production and processing equipment |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200121 |