CN219375530U - Sterilization inoculation device - Google Patents

Sterilization inoculation device Download PDF

Info

Publication number
CN219375530U
CN219375530U CN202320097786.8U CN202320097786U CN219375530U CN 219375530 U CN219375530 U CN 219375530U CN 202320097786 U CN202320097786 U CN 202320097786U CN 219375530 U CN219375530 U CN 219375530U
Authority
CN
China
Prior art keywords
inoculation
steam
bottle
pipeline
air
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202320097786.8U
Other languages
Chinese (zh)
Inventor
俞淑雅
周陈锋
杜明镜
张宇
邱学强
杭玉平
尹丽丽
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangsu Jiuyang Biological Pharmaceutical Co ltd
Original Assignee
Jiangsu Jiuyang Biological Pharmaceutical Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangsu Jiuyang Biological Pharmaceutical Co ltd filed Critical Jiangsu Jiuyang Biological Pharmaceutical Co ltd
Priority to CN202320097786.8U priority Critical patent/CN219375530U/en
Application granted granted Critical
Publication of CN219375530U publication Critical patent/CN219375530U/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The sterilizing and inoculating device relates to the technical field of inoculation bottle pipeline sterilization and comprises an inoculation bottle, wherein an air inlet mechanism and a discharging mechanism are arranged on the inoculation bottle, one end of the air inlet mechanism is connected with the inoculation bottle, and the other end of the air inlet mechanism is connected with an air component and a steam component; the discharging mechanism comprises a discharging pipeline, and one end of the discharging pipeline is also connected with the inoculating bottle. According to the utility model, the steam component communicated with the air component is arranged on the air inlet mechanism of the inoculation bottle, so that the air component can be sterilized through the steam component, and the inoculation bottle has a simple structure and high reliability.

Description

Sterilization inoculation device
Technical Field
The utility model relates to the technical field of sterilization of inoculation bottle pipelines, in particular to a sterilization inoculation device.
Background
Along with the development of the times, fermentation-extraction integrated workshops are becoming more and more popular, namely, one product needs to be built in the same production workshop so as to improve management and production efficiency. When such workshops are constructed, the explosion-proof problem related to the flame sterilization mode adopted by the old inoculation cannot be solved because the distance between the fermentation section and the extraction section is too short.
For example, chinese patent CN201621227296.1 discloses a seed tank inoculation device, which is provided with a film for sealing the inoculation port channel in the inoculation port, and adopts flame inoculation mode to inoculate, and the inoculation port space is sterilized by flame at high temperature to reduce the survival rate of viable bacteria in the space, thereby effectively avoiding the contamination of mixed bacteria. It still involves safety explosion protection issues.
There is a need to develop a sterilization inoculating apparatus to solve the problems of the prior art.
Disclosure of Invention
The utility model aims to provide a sterilization inoculation device without using flame in a workshop, so as to solve the problem that the safety hidden trouble exists in the workshop in a flame inoculation mode because the fermentation workshop section is too close to the extraction workshop section in the background technology.
In order to achieve the above purpose, the present utility model provides the following technical solutions:
the sterilization inoculation device comprises an inoculation bottle, wherein an air inlet mechanism and a discharging mechanism are arranged on the inoculation bottle, one end of the air inlet mechanism is connected with the inoculation bottle, and the other end of the air inlet mechanism is connected with an air component and a steam component; the discharging mechanism comprises a discharging pipeline, and one end of the discharging pipeline is also connected with the inoculating bottle.
Further, the air inlet mechanism comprises an air inlet pipeline, the air inlet pipeline is connected with the inoculation bottle, and a control valve is arranged on the air inlet pipeline; the discharging mechanism comprises a discharging pipeline, the discharging pipeline is connected with the inoculating bottle, and a control valve is also arranged on the discharging pipeline.
Further, the air assembly comprises an air pipeline, and a coarse filter, a fine filter and a control valve are sequentially arranged on the air pipeline.
Further, the steam component comprises a steam pipeline, and a steam filter and a control valve are sequentially arranged on the steam pipeline.
Further, the air pipe is connected with the air inlet pipe through a quick connection pipe, and the steam pipe is connected with the air inlet pipe through a quick connection pipe.
Further, the quick connection pipeline is a metal hose, and the control valve is provided with a discharge hole.
Further, a support frame is arranged on the inoculation bottle, and the support frame is detachably connected with the inoculation bottle.
Further, a supporting frame is also arranged on the steam component or the discharging mechanism.
Further, the sterilization inoculation device further comprises a seed tank, and an inoculation mechanism is arranged on the seed tank.
Further, a steam mechanism is further arranged on the seed tank and comprises a steam pipeline, and a control valve is arranged on the steam pipeline.
Compared with the prior art, the utility model has the beneficial effects that: through connect air subassembly and steam subassembly on the other end of mechanism that admits air, and air subassembly with steam subassembly is linked together, makes steam subassembly can be right pipeline in the air subassembly disinfects and sterilizes, is convenient for be connected with the inoculation bottle after accomplishing the disinfection inoculation in other workshops, avoids air subassembly when letting in the air for the inoculation bottle, because there is bacterium in the air inlet pipeline in it and pollute inoculation bottle seed liquid.
And the air inlet mechanism is communicated with the air inlet pipeline and the steam pipeline through quick connection pipelines respectively, so that the connection is quick and convenient, and the disassembly and assembly of the inoculation bottle are convenient. And this application uses the control valve who is provided with the discharge port, the steam disinfection of being convenient for.
Other features and advantages of the present utility model will be disclosed in the following detailed description of the utility model and the accompanying drawings.
Drawings
FIG. 1 is a schematic view of the overall structure of the present utility model;
FIG. 2 is a schematic diagram of the structure of an air module and a steam module;
fig. 3 is a schematic structural view of the seed tank of the present utility model.
The figure indicates: 1. inoculating a bottle; 11. an air inlet mechanism; 12. a discharging mechanism; 2. an air assembly; 21. a coarse filter; 22. fine filter; 3. a steam assembly; 31. a steam filter; 4. a control valve; 5. a quick connection pipeline; 6. and (5) supporting frames.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
In the present utility model, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.
The sterilization inoculation device, as shown in figures 1 to 3, comprises an inoculation bottle 1, wherein an air inlet mechanism 11 and a discharging mechanism 12 are arranged on the inoculation bottle 1,
the air inlet mechanism 11 comprises an air inlet pipeline, one end of the air inlet pipeline is connected with the inoculation bottle 1 and penetrates into the inoculation bottle 1 to be communicated with the inside of the inoculation bottle 1. The other end of the air inlet pipeline is provided with a control valve 5.
The discharging mechanism 12 comprises a discharging pipeline, one end of the discharging pipeline is also connected with the inoculating bottle 1, does not penetrate into the inoculating bottle 1, and is communicated with the bottle mouth of the inoculating bottle 1. The other end of the discharging pipeline is also provided with a control valve 5. Optionally, the air inlet mechanism 11 is connected with the air inlet of the inoculation bottle 1, and the discharging mechanism 12 is connected with the discharging hole of the inoculation bottle 1.
When the inoculation bottle 1 is inverted, the top surface of one end of the air inlet pipeline is higher than the top surface of one end of the discharging pipeline.
The other end of the air inlet mechanism 11 is connected with an air component 2 and a steam component 3, the other end of the air inlet pipeline can be provided with a three-way pipe fitting, and the air component 2 and the steam component 3 are respectively connected with two joints of the three-way pipe fitting.
The air assembly 2 comprises an air pipeline, and a coarse filter 21, a fine filter 22 and a control valve 5 are sequentially arranged on the air pipeline. One end of the air pipeline is connected with the air inlet pipeline, and the other end of the air pipeline is used for introducing air. The end of the air pipeline, which is connected with the air inlet pipeline, is the end provided with the control valve 5. The control valve 5 is preferably a diaphragm valve.
The steam component 3 comprises a steam pipeline, a steam filter 31 and a control valve 5 are sequentially arranged on the steam pipeline, one end of the steam pipeline is connected with the air inlet pipeline, and the other end of the steam pipeline is used for introducing steam. The end of the steam pipeline, which is connected with the air inlet pipeline, is the end provided with the control valve 5.
Optionally, the air pipe is connected to the air intake pipe through a quick connection pipe 6, and the steam pipe is connected to the air intake pipe through another quick connection pipe 6.
The sterilization inoculation device further comprises a seed tank 4, an inoculation mechanism 41 and a steam mechanism 42 are arranged on the seed tank 4, the inoculation mechanism 41 comprises an inoculation pipeline, and the inoculation pipeline is communicated with a discharging pipeline through a quick connection pipeline 6.
The steam mechanism 42 comprises a steam pipeline, one end of the steam pipeline is communicated with the seed tank 4, and the other end of the steam pipeline is provided with a control valve 5.
The quick-connect pipe 6 is preferably a metal hose. The quick connect pipe 6 can facilitate connection and replacement between pipes.
The inoculation bottle 1 is connected with a support frame 7, and the support frame 7 is detachably connected with the inoculation bottle 1. The steam component 3, the discharging mechanism 12 and the like can also be provided with a supporting frame 7. The supporting frame 7 can support the pipeline, the inoculating bottle 1 and the like.
When in use, the method comprises the following steps:
1. the inoculation bottle 1 and the two ends of the control valve 5 are pricked with gauze, and the gauze is assembled and put into a sterilizing cabinet for sterilizing the device. After sterilization, the seeds enter a clean area of the strains to finish the seed liquid operation on an ultra-clean workbench. And after the completion, the bottle is placed on a bottle rack of a new sterilization device and is transported to the site.
2. On site, the air inlet mechanism 11 of the inoculating bottle 1 is connected with the air component 2 (6 kg air source) and the steam component 3 through the quick connection pipe 6, the discharging mechanism 12 of the inoculating bottle 1 is connected with the inoculating mechanism 41 of the seed tank 4 through a hose (the valve on the inoculating mechanism 41 is operated to be in a closed state).
3. The lower discharge holes of the control valves 5 at both ends of the inoculation bottle 1 and the lower discharge holes of the control valves 5 in the air module 2 are opened. The control valve 5 in the steam assembly 3 and the control valve 5 in the steam mechanism 42 are opened, and steam is introduced to sterilize the air pipeline and the discharging pipeline of the inoculation bottle 1 for 15 minutes. After sterilization, the lower discharge hole of the control valve 5 of the air assembly 2 and the lower discharge hole of the control valves 5 at the two ends of the inoculation bottle 1 are closed, and then the control valve 5 of the total steam assembly 3 and the control valve 5 of the steam mechanism 42 are closed.
4. Firstly, the control valves 5 at the two ends of the inoculation bottle 1 are opened, then the air component 2 is opened to control the valves 5 (using air pressure) so as to quickly press the seed liquid in the inoculation bottle 1 into the seed tank 4 (estimated operation time is 15 seconds). After completion, the vapor mechanism 42 of the seeding tank 4 is closed to control the valve 5, and the air component 2 is closed to control the valve 5. And (5) detaching the connecting pipelines everywhere and leaving the site.
Optionally, the discharge port of the inoculating bottle 1 is provided with a sight glass to observe the seed liquid level.
The utility model provides a sterilization inoculation device which has the advantages of simple structure, convenient use and high reliability.
It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present utility model may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.

Claims (10)

1. The sterilization inoculation device is characterized by comprising an inoculation bottle, wherein an air inlet mechanism and a discharging mechanism are arranged on the inoculation bottle, one end of the air inlet mechanism is connected with the inoculation bottle, and the other end of the air inlet mechanism is connected with an air component and a steam component; the discharging mechanism comprises a discharging pipeline, and one end of the discharging pipeline is also connected with the inoculating bottle.
2. The sterilization and inoculation device according to claim 1, wherein the air inlet mechanism comprises an air inlet pipeline, the air inlet pipeline is connected with the inoculation bottle, and a control valve is arranged on the air inlet pipeline; the discharging mechanism comprises a discharging pipeline, the discharging pipeline is connected with the inoculating bottle, and a control valve is also arranged on the discharging pipeline.
3. The sterilization and inoculation device according to claim 2, wherein the air assembly comprises an air line on which a coarse filter, a fine filter and a control valve are sequentially provided.
4. A sterilization and inoculation device according to claim 3, wherein the steam assembly comprises a steam line on which a steam filter and a control valve are arranged in sequence.
5. The sterilization and inoculation device according to claim 4, wherein the air line is connected to the air intake line via a quick connect line, and the steam line is connected to the air intake line via a quick connect line.
6. The sterilization and inoculation device according to claim 5, wherein the quick-connect pipe is a metal hose, and the control valve is provided with a discharge hole.
7. The sterilization and inoculation apparatus according to claim 4, wherein the inoculation bottle is provided with a support frame, and the support frame is detachably connected with the inoculation bottle.
8. The sterilization and inoculation apparatus according to claim 7, wherein a support frame is also mounted to the steam assembly or the discharge mechanism.
9. The sterilization and inoculation device according to any one of claims 1 to 8, further comprising a seed tank, wherein the seed tank is provided with an inoculation mechanism.
10. The sterilization and inoculation device according to claim 9, wherein the seed tank is further provided with a steam mechanism comprising a steam line, and wherein the steam line is provided with a control valve.
CN202320097786.8U 2023-02-01 2023-02-01 Sterilization inoculation device Active CN219375530U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202320097786.8U CN219375530U (en) 2023-02-01 2023-02-01 Sterilization inoculation device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202320097786.8U CN219375530U (en) 2023-02-01 2023-02-01 Sterilization inoculation device

Publications (1)

Publication Number Publication Date
CN219375530U true CN219375530U (en) 2023-07-21

Family

ID=87190977

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202320097786.8U Active CN219375530U (en) 2023-02-01 2023-02-01 Sterilization inoculation device

Country Status (1)

Country Link
CN (1) CN219375530U (en)

Similar Documents

Publication Publication Date Title
CN219375530U (en) Sterilization inoculation device
CN214415283U (en) Steam-water mixing sterilizer of fermentation continuous sterilization system
CN101180936B (en) Inoculator of edible fungus
CN209122940U (en) A kind of health-caring capsule bactericidal unit
CN212894732U (en) Controllable inoculation equipment for microbial fermentation
CN202672242U (en) Water supply device with disinfection and sterilization function
CN212713507U (en) Filtering and sterilizing device for air inlet pipeline of fermentation tank
CN101153265B (en) Safe and portable liquid bacterial vaccinating device
CN215799512U (en) Algae oil DHA fermentation inoculation feed supplement device
CN201542986U (en) Sterilization apparatus for inoculation port of pilot fermentation tank
CN219279884U (en) Bioreactor inoculation system
CN1277456C (en) Method and apparatus for producing and inoculating liquid bacterial spawn in oxygenating mode
CN207605686U (en) A kind of improved liquid medicine dosing system for setting T-type liquid filter
CN201648371U (en) Needle tube feeding device for small-sized bioreactor
CN220300722U (en) Nitrile hydratase engineering strain fermentation air inlet sterilization device
CN213060846U (en) Promote convenient clear fast-assembling inoculation jar of sterilization effect
CN220284069U (en) Fermentation seed tank inoculation device
CN216136375U (en) Water needle sterilization cabinet
CN220494212U (en) Disinfection equipment for pre-packaged food
JPH10210965A (en) Aseptic starter inoculator
CN111001245A (en) Air treatment system of medical cleaning workstation and working method thereof
CN211652181U (en) Fermentation automatic sampling tube plate with air source valve island embedded in back plate
CN203235472U (en) Raw material sterile-feeding device in production of synthetic peptide vaccine for pig foot-and-mouth disease
CN220646127U (en) Vacuumizing device used in autoclave
CN220957184U (en) Sterile fluid is carried with wearing wall structure

Legal Events

Date Code Title Description
GR01 Patent grant
GR01 Patent grant