CN212595178U - A aseptic vacuum stirring filter equipment for preparation of fresh manure fungus liquid - Google Patents

A aseptic vacuum stirring filter equipment for preparation of fresh manure fungus liquid Download PDF

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Publication number
CN212595178U
CN212595178U CN202020535681.2U CN202020535681U CN212595178U CN 212595178 U CN212595178 U CN 212595178U CN 202020535681 U CN202020535681 U CN 202020535681U CN 212595178 U CN212595178 U CN 212595178U
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China
Prior art keywords
filtering
chamber
stirring
pipeline
tank body
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Expired - Fee Related
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CN202020535681.2U
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Chinese (zh)
Inventor
赵晋
柏明
孙世仁
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Air Force Medical University of PLA
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Air Force Medical University of PLA
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Priority to CN202020535681.2U priority Critical patent/CN212595178U/en
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Abstract

The utility model provides a sterile vacuum stirring and filtering device for preparing fresh liquid dung, which comprises a sealed tank body; the interior of the tank body is vertically separated by a partition plate to form a stirring chamber and a filtering chamber respectively; the left side wall of the stirring chamber is respectively provided with a sample input port and a cleaning device, the bottom end of the stirring chamber is provided with a liquid outlet, the right side wall of the stirring chamber is respectively connected with a vacuum pump and a steam autoclave, and a stirring blade in the stirring chamber is connected with a motor at the upper end of the tank body through a rotating shaft; the filtering chamber is connected with a vacuum pump, the lower end of the filtering chamber is provided with a liquid dung outlet, a plurality of filtering layers are arranged in the filtering chamber, and each filtering layer is provided with a discharge port; electromagnetic valves are arranged in the sample input port, the liquid discharge port, the cleaning device, the first pipeline, the second pipeline, the third pipeline, the fecal strain liquid outlet and the discharge port. The utility model discloses combine the step of excrement fungus preparation, whole device adopts intelligent control, and cleaning and disinfecting all is a key operation completion, and labour saving and time saving improves preparation efficiency.

Description

A aseptic vacuum stirring filter equipment for preparation of fresh manure fungus liquid
Technical Field
The utility model relates to the technical field of medical supplies, concretely relates to aseptic vacuum stirring filter equipment for preparation of fresh excrement fungus liquid.
Background
The coprophila transplantation technology is already mature and applied to the treatment of recurrent clostridium difficile infection and inflammatory bowel disease, and is gradually applied to the treatment of other systemic diseases (such as autism, alzheimer disease, hepatic encephalopathy, tumors and the like) except intestinal tracts. The demands of laboratories and clinics with different grades on fresh fecal bacteria liquid are increasing.
At present, fresh liquid dung is mainly prepared manually in a laboratory by laboratory personnel. However, 99% of the intestinal flora is anaerobic flora, and the intestinal flora is exposed to the air by manual stirring and filtering, so that the diversity and abundance of the flora are reduced, and the safety and effectiveness of the bacterial liquid are affected. Meanwhile, the manual stirring and the filtering of the excrement specimen greatly influence the physical and mental pleasure of experimenters, and cause pollution to the experimental environment to a certain extent. In addition, the instrument needs to be washed and disinfected before and after manual stirring and filtering, time and labor are wasted, and the supply efficiency is affected when the required amount of bacteria liquid is large.
In view of the foregoing, there is a need to design a sterile vacuum agitation filtration device for the preparation of fresh liquid manure that overcomes the above-mentioned problems.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a need not artifical stirring and filterable, wash and disinfect convenient aseptic vacuum stirring filter equipment who is used for fresh excrement fungus liquid preparation.
In order to realize the purpose, the utility model discloses a technical scheme is: an aseptic vacuum stirring and filtering device for preparing fresh fecal bacteria liquid comprises a sealed tank body;
the interior of the tank body is vertically separated by a partition plate to form a stirring chamber and a filtering chamber respectively;
a sample input port and a cleaning device are respectively arranged on the left side wall of the stirring chamber, a liquid discharge port communicated with the filtering chamber is arranged at the bottom end of the stirring chamber, a vacuum pump and a steam autoclave are respectively connected to the right side wall of the stirring chamber through a first pipeline and a second pipeline, and stirring blades in the stirring chamber are connected with a motor at the upper end of the tank body through a rotating shaft;
the filtering chamber is connected with a vacuum pump through a pipeline III, the lower end of the filtering chamber is provided with a fecal strain liquid outlet, a plurality of filtering layers are arranged inside the filtering chamber, and the side wall of the filtering chamber at the upper end of each filtering layer is respectively provided with a discharge port;
and electromagnetic valves are arranged in the sample input port, the liquid discharge port, the cleaning device, the first pipeline, the second pipeline, the third pipeline, the fecal strain liquid outlet and the discharge port.
The purpose of the utility model and the technical problem thereof can be further realized by adopting the following technical measures.
Furthermore, the cleaning device is an annular water spraying ring welded at the joint of the upper surface and the side wall of the tank body, a water injection port is arranged on the outer side of the annular water spraying ring, and the water injection port is communicated with an annular diversion trench arranged in the annular water spraying ring;
one side of the annular water spraying ring, which is far away from the water filling port, is uniformly distributed with a plurality of water spraying ports along the radial direction, one end of each water spraying port is communicated with the annular diversion trench, and the other end of each water spraying port is communicated with the inside of the tank body.
Furthermore, a port of the water spraying opening is provided with a filter screen protruding outwards.
Furthermore, the filtering holes of the filtering layers are sequentially reduced from top to bottom.
Furthermore, a first pressure sensor and a second pressure sensor are respectively arranged on the side walls of the stirring chamber and the filtering chamber.
Furthermore, a control panel is arranged on the upper surface of the tank body, and a microprocessor is arranged in the control panel;
and the microprocessor is respectively connected with the vacuum pump, the steam autoclave, the motor, the electromagnetic valve, the first pressure sensor and the second pressure sensor.
Compared with the prior art, the beneficial effects of the utility model are that:
1. the utility model discloses a sterile vacuum stirring and filtering device for preparing fresh fecal strain liquid, which comprises a sealed tank body, wherein the upper end in the tank body is a stirring chamber, the lower end is a filtering chamber, and stirring blades in the stirring chamber are driven to rotate by a motor, so that manual stirring is avoided, and physical and mental joyfulness of experimenters is influenced; and the vacuum pump is all connected to teeter chamber and filter chamber, and firstly guarantee jar internal vacuum or anaerobic state, improve intestinal canal flora's variety and richness, secondly can make the relative teeter chamber of filter chamber be in the negative pressure state, guarantee that the excrement fungus mixed liquid of teeter chamber falls the filter chamber fast to do not need artifical supplementary filtration, avoided the pollution to the environment to a certain extent.
2. The utility model discloses a sterile vacuum stirring and filtering device for preparing fresh fecal strain liquid, a cleaning device of the device is welded at the joint of the upper surface and the side wall of a tank body, a water filling port is arranged at the outer side of the cleaning device, and tap water can be communicated to directly wash the inside of the tank body; a plurality of water jet on the belt cleaning device can make jar internal portion wash evenly, avoids being infected with the filth on the stirring leaf, influences the secondary and uses.
3. The utility model discloses a sterile vacuum stirring filter equipment for preparation of fresh excrement fungus liquid, its jar body coupling steam autoclave can carry out the autoclaving disinfection to this device before wasing the back and using, and easy operation is convenient, and labour saving and time saving has improved work efficiency.
4. The utility model discloses a sterile vacuum stirring and filtering device for preparing fresh liquid dung, the upper surface of a tank body of the device is provided with a control panel, and a microprocessor is arranged in the control panel; the microprocessor is respectively connected with the vacuum pump, the steam autoclave, the motor, the electromagnetic valve and the pressure sensor, so that the whole intelligent control is realized, the operation is simple and fast, and the supply efficiency can be improved when the demand of the bacterial liquid is large.
Drawings
FIG. 1 is a schematic view of the overall structure of the sterile vacuum stirring and filtering device for preparing fresh fecal bacteria liquid provided by the present invention;
fig. 2 is a schematic view of a cut-away structure of a cleaning device in an embodiment of the present invention;
FIG. 3 is a schematic block diagram of the aseptic vacuum stirring and filtering device for preparing fresh fecal bacteria liquid provided by the present invention;
description of reference numerals:
1. a tank body; 11. a partition plate;
2. a stirring chamber; 21. a sample input port; 211. a first electromagnetic valve; 22. a liquid discharge port; 221. a second electromagnetic valve; 23. a cleaning device; 231. a water injection port; 232. an annular diversion trench; 233. a water jet; 234. filtering with a screen; 235. a third electromagnetic valve; 24. stirring blades; 25. a rotating shaft;
3. a filtering chamber; 31. a filter layer; 32. a liquid manure outlet; 321. a fourth electromagnetic valve; 33. a discharge outlet; 331. a fifth electromagnetic valve;
4. a vacuum pump; 41. a first pipeline; 411. a sixth electromagnetic valve; 42. a third pipeline; 421. a seventh electromagnetic valve;
5. a steam autoclave; 51. a second pipeline; 511. an eighth electromagnetic valve;
6. a motor;
7. a control panel;
8. a microprocessor;
9. a first pressure sensor;
10. and a second pressure sensor.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Referring to fig. 1-3, an aseptic vacuum stirring and filtering apparatus for preparing fresh fecal bacteria liquid includes a sealed tank 1;
the interior of the tank body 1 is vertically divided by a partition plate 11 to form a stirring chamber 2 and a filtering chamber 3 respectively;
a sample input port 21 and a cleaning device 23 are respectively arranged on the left side wall of the stirring chamber 2, a liquid discharge port 22 communicated with the filtering chamber 3 is arranged at the bottom end of the stirring chamber 2, a vacuum pump 4 and a steam autoclave 5 are respectively connected on the right side wall of the stirring chamber 2 through a first pipeline 41 and a second pipeline 51, and a stirring blade 24 in the stirring chamber 2 is connected with a motor 6 at the upper end of the tank body 1 through a rotating shaft 25;
the filter chamber 3 is connected with a vacuum pump 4 through a third pipeline 42, the lower end of the filter chamber is provided with a fecal bacteria liquid outlet 32, a plurality of filter layers 31 are arranged inside the filter chamber 3, and the side wall of the filter chamber 3 at the upper end of each filter layer 31 is respectively provided with a discharge port 33;
electromagnetic valves are arranged in the sample inlet 21, the liquid outlet 22, the cleaning device 23, the first pipeline 41, the second pipeline 51, the third pipeline 42, the liquid dung outlet 32 and the discharge outlet 33.
In the utility model, the inner part of the tank body 1 is respectively provided with the stirring chamber 2 and the filtering chamber 3 through the partition board 11, the structure is simple, and the volume of the whole device is reduced; the sample input port 21 is used for inputting the excrement sample into the stirring chamber 2 and injecting normal saline, and the motor 6 drives the rotating shaft 25 to drive the stirring blade 24 to rotate rapidly to form excrement mixed liquid; because 99% of the intestinal flora is anaerobic flora, the vacuum pump 4 is used for enabling the stirring chamber 2 to be in a vacuum or anaerobic state, so that the diversity and the richness of the intestinal flora are improved; the steam autoclave 5 is used for carrying out autoclave sterilization on the device after cleaning or before use, and has simple operation, time saving and labor saving; the fecal bacteria mixed liquid formed by stirring in the stirring chamber 2 is discharged into the filtering chamber 3 through the liquid outlet 22, in order to realize quick automatic filtering, the filtering chamber 3 is connected with the vacuum pump 4 through the third pipeline 42, so that the pressure in the filtering chamber 3 can be smaller than the pressure in the stirring chamber 2, the rapid filtering of the fecal bacteria mixed liquid is realized without manual assistance, and finally, the prepared fecal bacteria liquid is collected through the fecal bacteria liquid outlet 32; finally, the entire apparatus interior can be rinsed by the cleaning device 23 and autoclaved using the steam autoclave 5 for the next use. In order to control the inlet and outlet, electromagnetic valves are disposed inside the sample inlet 21, the liquid outlet 22, the cleaning device 23, the first pipe 41, the second pipe 51, the third pipe 42, the liquid dung outlet 32 and the material outlet 33. It should be noted that a first electromagnetic valve 211 is arranged in the sample inlet 21; a second electromagnetic valve 221 is arranged in the liquid outlet 22; a third electromagnetic valve 235 is arranged in the cleaning device 23; a six electromagnetic valve 411 is arranged in the first pipeline 41; an electromagnetic valve eight 511 is arranged in the second pipeline 51; a solenoid valve seven 421 is arranged in the pipeline three 42; a fourth electromagnetic valve 321 is arranged in the fecal bacteria liquid outlet 32; a fifth electromagnetic valve 331 is arranged in the discharge opening 33.
Preferably, the cleaning device 23 is an annular water spraying ring welded at the joint of the upper surface and the side wall of the tank body 1, a water injection port 231 is arranged outside the annular water spraying ring, and the water injection port 231 is communicated with an annular guide groove 232 arranged inside the annular water spraying ring; and a third electromagnetic valve 235 is arranged in the water injection port 231.
One side of the annular water spraying ring, which is far away from the water injection port 231, is uniformly distributed with a plurality of water injection ports 233 along the radial direction, one end of each water injection port 233 is communicated with the annular guide groove 232, and the other end of each water injection port 233 is communicated with the inside of the tank body 1.
In this embodiment, a water injection port 231 is disposed outside the annular water spraying ring, and can be communicated with a tap water pipe, so as to facilitate flushing and have sufficient flushing pressure; the annular guide groove 232 can lead tap water at the water injection ports 231 to be divided, so as to ensure that tap water is sprayed out from the plurality of water injection ports 233; because the cleaning device 23 is welded at the joint of the upper surface and the side wall of the tank body 1 and is an annular water spraying ring, the plurality of water spraying ports 233 can flush the stirring chamber 2 at different angles, and especially can flush away the feces stained on the stirring blades 24 quickly.
In order to ensure that the stirring chamber 2 is flushed more thoroughly, the end of the water jet 233 is provided with a filter 234 protruding outwards.
Preferably, the filtering holes of the filtering layers 31 are sequentially reduced from top to bottom, and the pore sizes of the filtering holes are sequentially 1.5mm, 1mm, 0.5mm and 0.2mm, so that sufficient filtering can be realized. The side walls of the filtering chambers 3 at the upper end of each filtering layer 31 are respectively provided with a discharge opening 33, solid dirt possibly remained on each filtering layer 3, and the discharge openings 33 are directly communicated with a sewer and are used for directly discharging the solid dirt into the sewer.
In order to control the pressure in the stirrer 2 and the filter chamber 3, a first pressure sensor 9 and a second pressure sensor 10 are respectively arranged on the side walls of the stirrer chamber 2 and the filter chamber 3. For example, when the stirring chamber 2 is close to a vacuum state, a pressure range can be set, and a pressure sensor 9 smaller or larger than the pressure range can transmit the acquired signal to the control unit; the filter chamber 3 is relatively negative in pressure, which should be smaller than the pressure range set by the stirring chamber 2, and if it is larger than the minimum value in this pressure range, the second pressure sensor 10 will also transmit the collected signal to the control unit.
Preferably, the upper surface of the tank body 1 is provided with a control panel 7, and a microprocessor 8 is arranged in the control panel 7;
the microprocessor 8 is respectively connected with the vacuum pump 4, the steam autoclave 5, the motor 6, the electromagnetic valve, the first pressure sensor 9 and the second pressure sensor 10.
In this embodiment, firstly, the microprocessor 8 controls to open the first electromagnetic valve 211 in the sample inlet 21, the experimenter injects the fecal sample and the physiological saline into the stirring chamber 2, and the microprocessor 8 controls to close the first electromagnetic valve 211; the first pressure sensor 9 transmits the acquired signals to the microprocessor 8, the microprocessor 8 compares the acquired signals with a set pressure range, if the acquired signals are smaller than or larger than the set pressure range, the vacuum pump 4 and the electromagnetic valve six 411 are controlled to be opened, and the pressure in the stirring chamber 2 is adjusted, so that the vacuum state in the stirring chamber 2 is ensured to be close to, and the bacterial colony is prevented from apoptosis; the microprocessor 8 controls the starting of the motor 6 to mix the excrement and the normal saline to form an excrement-bacterium mixed solution; next, the second pressure sensor 10 transmits the acquired signal to the microprocessor 8, the microprocessor 8 compares the acquired signal and controls the vacuum pump 4 to open to adjust the pressure, after the pressure is adjusted, the microprocessor 8 controls to open the second electromagnetic valve 221 in the liquid discharge port 22, so that the fecal bacteria mixed liquid is discharged into the filter chamber 3 for filtering, and after the pressure is adjusted, the microprocessor 8 controls to open the fourth electromagnetic valve 321 in the fecal bacteria liquid outlet 32, and the fecal bacteria stock solution is discharged for later use; the opening and closing of the steam autoclave 5 and the other solenoid valves are the same as the above-described principle and will not be described again. It should be noted that the microprocessor model is ARM9TDMI and the pressure sensor model is MIK-P300.
The present invention has been further described with reference to the embodiments, but the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.

Claims (6)

1. The utility model provides an aseptic vacuum stirring filter equipment for preparation of fresh manure fungus liquid, includes a sealed tank body (1), its characterized in that:
the interior of the tank body (1) is vertically separated by a partition plate (11) to form a stirring chamber (2) and a filtering chamber (3) respectively;
a sample inlet (21) and a cleaning device (23) are respectively arranged on the left side wall of the stirring chamber (2), a liquid outlet (22) communicated with the filtering chamber (3) is arranged at the bottom end of the stirring chamber, the right side wall of the stirring chamber (2) is respectively connected with a vacuum pump (4) and a steam autoclave (5) through a first pipeline (41) and a second pipeline (51), and a stirring blade (24) in the stirring chamber (2) is connected with a motor (6) at the upper end of the tank body (1) through a rotating shaft (25);
the filtering chamber (3) is connected with a vacuum pump (4) through a third pipeline (42), the lower end of the filtering chamber is provided with a fecal strain liquid outlet (32), a plurality of filtering layers (31) are arranged inside the filtering chamber (3), and the side wall of the filtering chamber (3) at the upper end of each filtering layer (31) is respectively provided with a discharge port (33);
the sample inlet (21), the liquid outlet (22), the cleaning device (23), the first pipeline (41), the second pipeline (51), the third pipeline (42), the fecal strain liquid outlet (32) and the discharge port (33) are internally provided with electromagnetic valves.
2. The sterile vacuum stirring and filtering device for preparing fresh fecal bacteria liquid as claimed in claim 1, wherein:
the cleaning device (23) is an annular water spraying ring welded at the joint of the upper surface and the side wall of the tank body (1), a water injection port (231) is arranged on the outer side of the annular water spraying ring, and the water injection port (231) is communicated with an annular flow guide groove (232) arranged in the annular water spraying ring;
one side of the annular water spraying ring, which is far away from the water injection port (231), is uniformly provided with a plurality of water spraying ports (233) along the radial direction, one end of each water spraying port (233) is communicated with the annular diversion trench (232), and the other end of each water spraying port (233) is communicated with the inside of the tank body (1).
3. The sterile vacuum stirring filter device for preparing fresh fecal bacteria liquid as claimed in claim 2, wherein the port of the water spraying port (233) is provided with a filter screen (234) protruding outwards.
4. The sterile vacuum stirring filter device for preparing fresh fecal bacteria liquid as claimed in claim 1, wherein the filter holes of the plurality of filter layers (31) decrease sequentially from top to bottom.
5. The sterile vacuum stirring and filtering device for preparing fresh fecal bacteria liquid as claimed in claim 1, wherein the side walls of the stirring chamber (2) and the filtering chamber (3) are respectively provided with a first pressure sensor (9) and a second pressure sensor (10).
6. The sterile vacuum stirring and filtering device for preparing fresh fecal bacteria liquid as claimed in claim 1, wherein:
a control panel (7) is arranged on the upper surface of the tank body (1), and a microprocessor (8) is arranged in the control panel (7);
and the microprocessor (8) is respectively connected with the vacuum pump (4), the steam autoclave (5), the motor (6), the electromagnetic valve, the first pressure sensor (9) and the second pressure sensor (10).
CN202020535681.2U 2020-04-13 2020-04-13 A aseptic vacuum stirring filter equipment for preparation of fresh manure fungus liquid Expired - Fee Related CN212595178U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202020535681.2U CN212595178U (en) 2020-04-13 2020-04-13 A aseptic vacuum stirring filter equipment for preparation of fresh manure fungus liquid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202020535681.2U CN212595178U (en) 2020-04-13 2020-04-13 A aseptic vacuum stirring filter equipment for preparation of fresh manure fungus liquid

Publications (1)

Publication Number Publication Date
CN212595178U true CN212595178U (en) 2021-02-26

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114028867A (en) * 2021-11-08 2022-02-11 武汉市农业科学院 Quick extraction element of animal manure fungus crowd based on anaerobic condition
CN114832406A (en) * 2022-04-19 2022-08-02 李博 Extraction and concentration device of frankincense extract for cosmetics
CN114836316A (en) * 2022-05-10 2022-08-02 奇辉生物科技(扬州)有限公司 Intestinal content draws supervision device

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114028867A (en) * 2021-11-08 2022-02-11 武汉市农业科学院 Quick extraction element of animal manure fungus crowd based on anaerobic condition
CN114832406A (en) * 2022-04-19 2022-08-02 李博 Extraction and concentration device of frankincense extract for cosmetics
CN114836316A (en) * 2022-05-10 2022-08-02 奇辉生物科技(扬州)有限公司 Intestinal content draws supervision device

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Granted publication date: 20210226