CN210560379U

CN210560379U - Inoculation device

Info

Publication number: CN210560379U
Application number: CN201921183340.7U
Authority: CN
Inventors: 高鹏
Original assignee: Laiwu Taihe Biochemistry Co ltd
Current assignee: Laiwu Taihe Biochemistry Co ltd
Priority date: 2019-07-25
Filing date: 2019-07-25
Publication date: 2020-05-19
Anticipated expiration: 2029-07-25

Abstract

The utility model provides an inoculation device, including aseptic water pitcher, with the inoculation cup of aseptic water pitcher intercommunication, with the inoculation jar of inoculation cup intercommunication, with the inoculation collection box of inoculation jar intercommunication. When inoculating, the inoculating branch valve and the inoculating main valve are opened, air in the inoculating tank enters the device, and spore or thallus suspension flows into the inoculating tank by self gravity. The utility model avoids the pressure relief of the inoculation tank to normal atmospheric pressure during the inoculation period, and avoids the pollution hidden danger caused by the pressure relief; the steps are simplified, and the workload is reduced; the storage time of spore or thallus suspension is shortened, and the suspension can be poured at any time.

Description

Inoculation device

Technical Field

The utility model relates to an inoculating device, in particular to an inoculating device for spores or thalli.

Background

In modern fermentation engineering, inoculation is a very important link and is also a link which is very easy to careless. The inoculation device adopted by most of the enterprises at present is shown in figure 1, and the inoculation mode is as follows: the fermenter (or seed tank) is depressurized to 0, then the flame is ignited at the mouth of the inoculation cup, and the seeds are poured into the fermenter (or seed tank) in a flame-sealed manner under non-sterile conditions.

First, the tank pressure of the tank to be inoculated has to be reduced to zero in the inoculation process, and the inoculation cup mouth can be opened and the seed liquid is poured in. However, when the pressure of the tank is reduced to zero, the external bacteria have an opportunity to enter the fermentation tank through a plurality of positions, such as an air outlet, a mechanical seal or some unknown leakage points, and the like, so that the pollution is caused. Secondly, the flame is ignited at the mouth of the inoculation cup, and the inoculation is carried out in a flame sealing mode, so that a small amount of dust and mixed bacteria can be still attached to the poured seed liquid and brought into a fermentation tank, and the pollution is caused. Thirdly, the seeds are poured into a sterile container in a sterile room for preparing the suspension, and then the suspension is inoculated in a fermentation tank, so that the seed liquid needs to be prepared in advance, the steps of storing, transporting and the like of the spore liquid are added, the process is complicated, and uncertain factors of pollution are increased.

SUMMERY OF THE UTILITY MODEL

The utility model provides an inoculation device aiming at the defects of the prior art to solve the problems in the background art.

The utility model is realized by the following technical proposal, and provides an inoculation device which comprises an aseptic water tank, an inoculation cup communicated with the aseptic water tank, an inoculation tank communicated with the inoculation cup, and an inoculation header communicated with the inoculation tank;

a connecting pipeline between the sterile water tank and the inoculation cup is provided with a sterile water valve and an isolating valve, a pipeline between the sterile water valve and the isolating valve is communicated with a steam exhaust pipe, and the steam exhaust pipe is provided with a steam exhaust valve;

the inoculation cup is positioned in the sterile room and comprises an inoculation cover and a cup body, the inoculation cover is detachably connected with the cup body, and the inoculation cover is movably connected with the sterile water tank;

the inoculation tank with be provided with the inoculation main valve on the pipeline between the inoculation header, a plurality of inoculation pipes of inoculation header intercommunication, all be provided with on the inoculation pipe and connect the seed dividing valve.

Preferably, the communication pipeline is a stainless steel pipeline.

Preferably, the sterile water tank, the inoculation cup, the inoculation tank and the inoculation header are all made of stainless steel.

Preferably, the sterile water tank, the inoculation cup, the inoculation tank and the connecting pipeline thereof are all provided with a plurality of sets.

Preferably, the inoculation cover is fixedly connected with the cup body through threads.

The embodiment of the utility model provides a technical scheme can contain following beneficial effect:

the utility model provides an inoculation device, which comprises an aseptic water tank, an inoculation cup communicated with the aseptic water tank, an inoculation tank communicated with the inoculation cup, and an inoculation header communicated with the inoculation tank; a connecting pipeline between the sterile water tank and the inoculation cup is provided with a sterile water valve and an isolating valve, a pipeline between the sterile water valve and the isolating valve is communicated with a steam exhaust pipe, and the steam exhaust pipe is provided with a steam exhaust valve; the inoculation cup is positioned in the sterile room and comprises an inoculation cover and a cup body, the inoculation cover is detachably connected with the cup body, and the inoculation cover is movably connected with the sterile water tank; the inoculation tank with be provided with the inoculation main valve on the pipeline between the inoculation header, a plurality of inoculation pipes of inoculation header intercommunication, all be provided with on the inoculation pipe and connect the seed dividing valve.

Before inoculation, the inoculation branch valve, the inoculation main valve, the closing valve and the steam exhaust valve are opened, the sterile water valve is closed, exhaust is conducted out of a sterile room, sterile air is introduced from the inoculation branch valve to blow cold after disinfection is finished, the inoculation branch valve and the inoculation main valve are closed after the equipment is blown cold, pressure is relieved to no pressure in the equipment through the steam exhaust valve, the closing valve and the steam exhaust valve are closed, the inoculation cover is screwed down, spore or thallus suspension is poured into the inoculation tank through the inoculation cup, the inoculation cover is screwed up after the inoculation is finished, the inoculation branch valve and the inoculation main valve are opened during inoculation, air in the inoculation tank enters the equipment, and the spore or thallus suspension flows into the inoculation tank through self gravity. The inoculation cup of the utility model is positioned in the sterile room, thereby avoiding the occurrence of the condition that dust and sundry bacteria are stained on the suspension and enter the inoculation tank; and all parts are connected in a sealing way, so that the pressure of the inoculation tank is prevented from being released to normal atmospheric pressure during inoculation, and the hidden pollution trouble caused by pressure release is avoided; make the suspension originally among the prior art and pour the seed into aseptic container in aseptic chamber, go the fermentation tank inoculation again, the utility model discloses can directly pour into the attached inoculation jar on the fermentation tank, direct access has simplified original work step, has reduced work load.

Drawings

For a clearer explanation of the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.

FIG. 1 is a schematic view of a conventional inoculating device.

Fig. 2 is a schematic structural diagram of an inoculation device provided by an embodiment of the present invention.

Shown in the figure: 1-sterile water tank, 2-sterile water valve, 3-exhaust valve, 4-isolating valve, 5-inoculation cover, 6-inoculation cup, 7-inoculation tank, 7-inoculation main valve, 8-inoculation header, 9-inoculation header and 10-inoculation valve.

Detailed Description

In order to make the technical solutions in the present invention better understood, the embodiments of the present invention will be described clearly and completely below, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those skilled in the art without creative efforts shall belong to the protection scope of the present invention.

Referring to fig. 2, a schematic structural diagram of an inoculating device according to an embodiment of the present invention is shown.

As can be seen from FIG. 2, the inoculation device comprises a sterile water tank 1, an inoculation cup 6 communicated with the sterile water tank 1, an inoculation tank 7 communicated with the inoculation cup 6, and an inoculation header 9 communicated with the inoculation tank 7;

a communication pipeline between the sterile water tank 1 and the inoculation cup 6 is provided with a sterile water valve 2 and an isolating valve 4, a pipeline between the sterile water valve 2 and the isolating valve 4 is communicated with a steam exhaust pipe, and the steam exhaust pipe is provided with a steam exhaust valve 3;

the inoculation cup 6 is positioned in an aseptic chamber, the inoculation cup 6 comprises an inoculation cover 5 and a cup body, the inoculation cover 5 is fixedly connected with the cup body through threads, and the inoculation cover 5 is movably connected with the aseptic water tank 1;

the inoculation tank 7 with be provided with inoculation main valve 8 on the pipeline between the inoculation collection box 9, inoculation collection box 9 communicates a plurality of inoculation pipes, all be provided with on the inoculation pipe and inoculate seed valve 10.

Sterile water is filled in the sterile water tank 1, and the sterile water can be obtained by closing the valve 4, opening the valves 2 and 3 and introducing steam to sterilize tap water from the valve 3. The connecting pipelines are stainless steel pipelines, and the inoculation cup 6 is placed on a sterile operating platform of a sterile room for sterile operation. The sterile water tank 1, the inoculation cup 6, the inoculation tank 7 and the inoculation header 9 are all made of stainless steel materials and can be sterilized at high temperature and high pressure. The sterile water tank 1, the inoculation cup 6, the inoculation tank 7 and the connecting pipelines thereof are all multiple and can be used alternately, and the inoculation valves 10 are multiple and are used for respectively inoculating corresponding to the multiple tank bodies.

The utility model provides an inoculation device, inoculation cup 6 must be located aseptic indoor, sealing connection between each department, the steam extraction mouth at exhaust valve 3 place is located aseptic outdoor, aseptic water pitcher 1 can be located aseptic indoor, also can be located ordinary indoor, the pipeline at pipeline, the main valve 8 place of inoculation jar 7, the main valve 10 place of inoculation, inoculation collection case 9 all can be located aseptic indoor also can be located aseptic outdoor, and the pipeline, the main valve 8 place of inoculation of pipeline, inoculation jar 7, the branch valve 10 place of inoculation jar 1, inoculation jar 7, the work of this embodiment in order to reduce aseptic indoor each supplementary utensil, and the collection case 9 of inoculation all sets up in aseptic outdoor.

The inoculation device provided by the embodiment is characterized in that before inoculation, firstly, sterilization is carried out, the inoculation sub-valve 10, the inoculation main valve 8, the isolating valve 4 and the steam exhaust valve 3 are opened, the sterile water valve 2 is closed, the device is sterilized, and exhaust gas is led out of a sterile room through a pipeline where the steam exhaust valve 3 and the steam exhaust valve 3 are located. After the disinfection is finished, sterile air is introduced from the inoculation branch valve 10 to blow cold, after the equipment blows cold, the inoculation branch valve 10 and the inoculation main valve 8 are closed, the pressure is relieved to no pressure in the equipment through the exhaust valve 3, the closing valve 4 and the exhaust valve 3 are closed, the inoculation cover 5 at the operating platform of the sterile room is screwed off, the suspension of spores or thalli is poured into the inoculation tank 7 through the inoculation cup 6, and the inoculation cover 5 is screwed on after the completion. The utility model discloses an inoculation cup 6 is located aseptic chamber, has avoided dust, miscellaneous fungus to be stained with the condition emergence of going into in the inoculation jar on the suspension.

When inoculating, the valve inoculating valve 10 and the inoculating main valve 8 are opened, air in the inoculating tank 7 enters the device, and spore or thallus suspension flows into the inoculating tank 7 by self gravity.

After inoculation is finished, the sterile water valve 2 and the isolating valve 4 can be opened, and the spores or thalli adhered to the inner wall are flushed into the tank to be inoculated by sterile water, so that the cleaning effect is achieved. And repeating the steps when inoculating the next time.

In the utility model, the inoculation cup 6 is used as an auxiliary inoculation tank of the inoculation tank 7, spore or thallus suspension is poured into the inoculation tank 7 through the inoculation cup 6, namely the suspension is directly poured into the auxiliary inoculation tank on the fermentation tank for direct access; compared with the prior inoculation technology, the method has the advantages that seeds are poured into the sterile container in the sterile room and then transferred to the fermentation tank for inoculation, the seed liquid does not need to be prepared in advance, the processes of storage, transportation and the like of the spore liquid are saved, the process is simple, and the possible pollution in the process is avoided.

The utility model provides an inoculation device is sealing connection between each part, can avoid treating that the inoculation jar releases pressure to normal atmospheric pressure during the inoculation, has reduced the pollution hidden danger because of the pressure release exists. Make the suspension originally among the prior art and pour the seed into aseptic container in aseptic chamber, go the fermentation tank inoculation again, the utility model discloses can directly pour into the attached inoculation jar on the fermentation tank, direct access has simplified original work step, has reduced work load. The storage time of spore or thallus suspension is shortened, and the suspension can be poured at any time.

Of course, the above description is not limited to the above examples, and technical features of the present invention that are not described in the present application may be implemented by or using the prior art, and are not described herein again; the above embodiments are only used for illustrating the technical solutions of the present invention and are not intended to limit the present invention, and the present invention has been described in detail with reference to the preferred embodiments, and those skilled in the art should understand that changes, modifications, additions or substitutions made by those skilled in the art within the spirit of the present invention do not depart from the spirit of the present invention, and shall also belong to the protection scope of the claims of the present invention.

Claims

1. An inoculation device is characterized by comprising a sterile water tank (1), an inoculation cup (6) communicated with the sterile water tank (1), an inoculation tank (7) communicated with the inoculation cup (6), and an inoculation header (9) communicated with the inoculation tank (7);

a communication pipeline between the sterile water tank (1) and the inoculation cup (6) is provided with a sterile water valve (2) and an isolating valve (4), a pipeline between the sterile water valve (2) and the isolating valve (4) is communicated with a steam exhaust pipe, and the steam exhaust pipe is provided with a steam exhaust valve (3);

the inoculation cup (6) is positioned in a sterile room, the inoculation cup (6) comprises an inoculation cover (5) and a cup body, the inoculation cover (5) is detachably connected with the cup body, and the inoculation cover (5) is movably connected with the sterile water tank (1);

the inoculation tank (7) and be provided with inoculation main valve (8) on the pipeline between inoculation collection box (9), inoculation collection box (9) intercommunication a plurality of inoculation pipes, all be provided with on the inoculation pipe and inoculate seed valve (10).

2. The inoculating device as claimed in claim 1, wherein the communicating pipes are stainless steel pipes.

3. An inoculating device according to claim 2, characterized in that the sterile water tank (1), the inoculating cup (6), the inoculating tank (7) and the inoculating header (9) are all stainless steel.

4. An inoculating device according to claim 3, characterized in that the sterile water tank (1), the inoculating cup (6), the inoculating tank (7) and their connecting pipes are all in plurality.

5. An inoculating device according to claim 4, characterised in that the inoculating cap (5) is in threaded fixed connection with the cup body.