CN207143241U - Differential pressure is inoculated with formula bioreactor - Google Patents
Differential pressure is inoculated with formula bioreactor Download PDFInfo
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- CN207143241U CN207143241U CN201721050904.0U CN201721050904U CN207143241U CN 207143241 U CN207143241 U CN 207143241U CN 201721050904 U CN201721050904 U CN 201721050904U CN 207143241 U CN207143241 U CN 207143241U
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- inoculation
- bioreactor
- seal box
- inoculated
- biological respinse
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Abstract
The utility model discloses differential pressure to be inoculated with formula bioreactor, including seal box and biological respinse case, the seal box is provided with inoculation mouth, access flap and magnet, steam pipe is provided with the seal box, blast pipe and dividing plate, the steam pipe is provided with steam pipe valve, the blast pipe is provided with blow off valve, the dividing plate is provided with sealing ring, adapter is provided with the biological respinse case, bioreactor and rubber blanket, the adapter is provided with nozzle valve, the biological respinse case is provided with support block, the differential pressure is inoculated with formula bioreactor, inoculation mouth sterilizes without secondary sterilization, enhance the security of inoculation；It will not cause to scald during inoculation, safe operation, access flap be removed and can be inoculated with during inoculation, it is easy to operate, there is good value.
Description
Technical field
It the utility model is related to bioreactor technology field, specially differential pressure inoculation formula bioreactor.
Background technology
, it is necessary to cultivate a number of pure microorganism fungus kind under sterile environment before microbial fermentation starts, work as life
The culture medium that has configured of thing reactor (or fermentation tank) input, by high-temperature sterilization, then it is cooled to the temperature of technological requirement
When, the process ready microorganism fungus kind being transferred in bioreactor is to be inoculated with.The operation of inoculation is needed sterile
In the state of complete, otherwise can cause living contaminants so as to cause fermentation fail, therefore just need classification inoculation apparatus inoculation mouth
Around produce gnotobasis for inoculation operation smoothly complete.
The inoculation method often used on bioreactor mainly has flame inoculation method and differential pressure inocalation method.Flame inoculation method
A circle combustible is placed usually around the inoculation mouth of bioreactor, combustible is ignited rear caused flame by inoculation mouth
Surround, at this moment open inoculation mouth and quickly pour into ready strain in reactor, close inoculation mouth and then extinguish combustible.
The flame of high temperature produces a sterile subenvironment around inoculation mouth, and being inoculated with this subenvironment can prevent substantially
The danger of living contaminants.But the shortcomings that flame inoculation method is also obvious.Be exactly first require operator's a sleight of hand,
Action is quick, opens and holds the container of strain and topple in the operation of strain and to ensure that the opening of container and inoculation mouth are placed in all the time
Under the protection of flame, if misoperation or action are too slow, all strain may be caused to be directly exposed to cause to contaminate in air
Bacterium；Further, since the temperature of flame is very high, both hands all the time will be very close to flame, so very in operating process is inoculated with by operator
Easily cause and burn.Differential pressure inocalation method be it is another relatively through frequently with inoculation method, it is mainly by adjusting reactor
Air inlet and exhaust bioreactor and hold the pressure inside the container of strain to control, make the inside of container for holding strain
Pressure is higher than the internal pressure of bioreactor, so as to make ready strain be inoculated into bioreactor in the presence of pressure difference
It is interior.
The inoculation mouth of the differential pressure inoculating device typically used at present is all directly exposed in air, every time before inoculation all
Inoculation mouth is carried out disinfection again, waste steam, and waste time.Further, since secondary sterilization will be opened immediately after terminating
Begin to be inoculated with, inoculation mouth temperature is very high, operating difficulties, and easily causes scald.And connect because steam can not be reached directly
The outer wall of kind mouth, heat transfer can only be relied on to sterilize the outer wall of inoculation mouth, cause sterilization not thorough.
Utility model content
The purpose of this utility model is to provide differential pressure inoculation formula bioreactor, solved proposed in background technology
Problem.
To achieve the above object, the utility model provides following technical scheme：Differential pressure is inoculated with formula bioreactor, including close
Joint sealing and biological respinse case, the seal box are provided with inoculation mouth, access flap and magnet, be provided with the seal box steam pipe,
Blast pipe and dividing plate, the steam pipe are provided with steam pipe valve, and the blast pipe is provided with blow off valve, and the dividing plate is provided with
Sealing ring, the biological respinse case is interior to be provided with adapter, bioreactor and rubber blanket, and the adapter is provided with nozzle valve, described
Biological respinse case is provided with support block.
As a kind of preferred embodiment of the present utility model, the inoculation mouth is arranged on middle above seal box, and
It is fixedly connected with sealing ring and dividing plate, the dividing plate is arranged at inoculation mouth both sides, forms groove, and the magnet is located at inoculation mouth
Circumference, and be fixedly connected with seal box, the access flap is arranged on above inoculation mouth, and is detachably connected with magnet, described to connect
Lid is made using iron material matter.
As a kind of preferred embodiment of the present utility model, at the steam pipe is arranged on the right side of inoculation mouth, and with every
Plate is fixedly connected with seal box, and the steam pipe valve is arranged on the right side of seal box, and is flexibly connected with steam pipe.
As a kind of preferred embodiment of the present utility model, the blast pipe is arranged at inoculation mouth left side, and with connecing
Kind mouth is fixedly connected with seal box, and the blow off valve is arranged at seal box left side, and is flexibly connected with blast pipe.
As a kind of preferred embodiment of the present utility model, the seal box is arranged at biological respinse case top, and
It is fixedly connected by support block with biological respinse case, the bioreactor is arranged on biological respinse case lower inner wall middle, and
It is fixedly connected by rubber blanket with bioreactor, the adapter is arranged on above bioreactor, and with nozzle valve activity even
Connect.
Compared with prior art, the beneficial effects of the utility model are as follows：Inoculation mouth sterilizes without secondary sterilization, enhances
The security of inoculation；The access flap being provided with, it will not cause to scald during inoculation, safe operation, removing access flap during inoculation can
Inoculation, it is easy to operate, there is good value.
Brief description of the drawings
Fig. 1 is the main structure diagram that the utility model differential pressure is inoculated with formula bioreactor；
Fig. 2 is the cross section structure diagram that the utility model differential pressure is inoculated with formula bioreactor.
In figure:1- seal boxs, 11- inoculation mouths, 12- steam pipes, 121- steam pipe valves, 13- blast pipes, 131- blast pipes
Valve, 14- access flaps, 15- magnet, 16- dividing plates, 161- sealing rings, 2- biological respinse casees, 21- adapters, 211- nozzle valves, 22- lifes
Thing reactor, 23- rubber blankets, 3- support blocks.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment obtained, belong to the scope of the utility model protection.
Fig. 1-2 is referred to, the utility model provides a kind of technical scheme：Differential pressure is inoculated with formula bioreactor, including sealing
Case 1 and biological respinse case 2, the seal box 1 are provided with inoculation mouth 11, access flap 14 and magnet 15, are provided with the seal box 1
Steam pipe 12, blast pipe 13 and dividing plate 16, the steam pipe 12 are provided with steam pipe valve 121, and the blast pipe 13 is provided with row
Tracheae valve 131, the dividing plate 16 are provided with sealing ring 161, adapter 21, bioreactor 22 are provided with the biological respinse case 2
With rubber blanket 23, the adapter 21 is provided with nozzle valve 211, and the biological respinse case 2 is provided with support block 3.
The inoculation mouth 11 is arranged on the top middle of seal box 1, and is fixedly connected with sealing ring 161 and dividing plate 16, institute
State dividing plate 16 to be arranged at the both sides of inoculation mouth 1, form groove, the magnet 15 is located at the circumference of inoculation mouth 1, and solid with seal box 1
Fixed connection, the access flap 14 is arranged on the top of inoculation mouth 1, and is detachably connected with magnet 15, and the access flap 14 uses iron
Material is made；It the access flap being provided with, will not cause to scald during inoculation, safe operation, access flap be removed and can be inoculated with during inoculation,
It is easy to operate, there is good value；The access flap 14 being provided with connects with the absorption of magnet 15, simple in construction, simple to operate.
The steam pipe 12 is arranged at the right side of inoculation mouth 11, and is fixedly connected with dividing plate 16 and seal box 1, the steam
Pipe valve 121 is arranged on the right side of seal box 1, and is flexibly connected with steam pipe 12；The blast pipe 12 being provided with, passes through steam pipe valve 121
Control steam to enter at inoculation mouth 11, the dividing plate and sealing ring that are provided with, further improve steam and leak, make inoculation mouth without two
Secondary sterilization, enhance the security of inoculation.
The blast pipe 13 is arranged at the left side of inoculation mouth 11, and is fixedly connected with inoculation mouth 11 and seal box 1, the row
Tracheae valve 131 is arranged at the left side of seal box 1, and is flexibly connected with blast pipe 13；The blast pipe 13 being provided with, for discharging steam
With the discharge of condensed water.
The seal box 1 is arranged at the top of biological respinse case 2, and is fixed and connected by support block 3 and biological respinse case 3
Connect, the bioreactor 22 is arranged on the lower inner wall middle of biological respinse case 2, and passes through rubber blanket 23 and bioreactor 22
It is fixedly connected, the adapter 21 is arranged on the top of bioreactor 22, and is flexibly connected with nozzle valve 211；The support block being provided with
3, it is simple in construction, provided with rubber blanket 23, play a part of a protection bioreactor 22.
Operation principle：The utility model when in use, first, access flap 14 and the absorption connection of magnet 15, makes inoculation mouth 11
Place forms hollow state, opens steam pipe valve 121, enters steam, carry out disinfection, steam caused by sterilization and condensed water pass through
Open blow off valve 131 to discharge from blast pipe 13, during inoculation, steam off pipe valve 131 and blow off valve 121, open nozzle valve
211, carry out biological respinse.
Finally it should be noted that：Preferred embodiment of the present utility model is the foregoing is only, is not limited to this
Utility model, although the utility model is described in detail with reference to the foregoing embodiments, for those skilled in the art
For, it can still modify to the technical scheme described in foregoing embodiments, or to which part technical characteristic
Carry out equivalent substitution.All any modification, equivalent substitution and improvements within the spirit and principles of the utility model, made etc.,
It should be included within the scope of protection of the utility model.
Claims (5)
1. differential pressure is inoculated with formula bioreactor, including seal box (1) and biological respinse case (2), it is characterised in that：The seal box
(1) inoculation mouth (11), access flap (14) and magnet (15) are provided with, steam pipe (12), blast pipe are provided with the seal box (1)
(13) and dividing plate (16), the steam pipe (12) are provided with steam pipe valve (121), and the blast pipe (13) is provided with blow off valve
(131), the dividing plate (16) is provided with sealing ring (161), and adapter (21), biological respinse are provided with the biological respinse case (2)
Device (22) and rubber blanket (23), the adapter (21) are provided with nozzle valve (211), and the biological respinse case (2) is provided with support
Block (3).
2. differential pressure according to claim 1 is inoculated with formula bioreactor, it is characterised in that：The inoculation mouth (11) is arranged on
Middle above seal box (1), and be fixedly connected with sealing ring (161) and dividing plate (16), the dividing plate (16) is arranged on inoculation
At mouth (11) both sides, groove is formed, the magnet (15) is located at inoculation mouth (11) circumference, and is fixedly connected with seal box (1), institute
State access flap (14) to be arranged on above inoculation mouth (11), and be detachably connected with magnet (15), the access flap (14) uses iron
Material is made.
3. differential pressure according to claim 1 is inoculated with formula bioreactor, it is characterised in that：The steam pipe (12) is arranged on
At on the right side of inoculation mouth (11), and it is fixedly connected with dividing plate (16) and seal box (1), the steam pipe valve (121) is arranged on sealing
On the right side of case (1), and it is flexibly connected with steam pipe (12).
4. differential pressure according to claim 1 is inoculated with formula bioreactor, it is characterised in that：The blast pipe (13) is arranged on
At on the left of inoculation mouth (11), and it is fixedly connected with inoculation mouth (11) and seal box (1), the blow off valve (131) is arranged on close
At on the left of joint sealing (1), and it is flexibly connected with blast pipe (13).
5. differential pressure according to claim 1 is inoculated with formula bioreactor, it is characterised in that：The seal box (1) is arranged on
At above biological respinse case (2), and it is fixedly connected by support block (3) with biological respinse case (2), the bioreactor (22)
Biological respinse case (2) lower inner wall middle is arranged on, and is fixedly connected by rubber blanket (23) with bioreactor (22), it is described
Adapter (21) is arranged on above bioreactor (22), and is flexibly connected with nozzle valve (211).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721050904.0U CN207143241U (en) | 2017-08-22 | 2017-08-22 | Differential pressure is inoculated with formula bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721050904.0U CN207143241U (en) | 2017-08-22 | 2017-08-22 | Differential pressure is inoculated with formula bioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207143241U true CN207143241U (en) | 2018-03-27 |
Family
ID=61667127
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721050904.0U Active CN207143241U (en) | 2017-08-22 | 2017-08-22 | Differential pressure is inoculated with formula bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207143241U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113136312A (en) * | 2021-05-11 | 2021-07-20 | 侯磊 | Inoculation equipment for biological fermentation and biological fermentation process |
-
2017
- 2017-08-22 CN CN201721050904.0U patent/CN207143241U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113136312A (en) * | 2021-05-11 | 2021-07-20 | 侯磊 | Inoculation equipment for biological fermentation and biological fermentation process |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 610000 Beijing Road, Chengdu economic and Technological Development Zone, Sichuan 182 Patentee after: Chengdu Hong Wah biological products Limited by Share Ltd Address before: 610000 Beijing Road, Chengdu economic and Technological Development Zone, Sichuan 182 Patentee before: Chengdu Kanghua Biological Products Co., Ltd. |