CN210130164U - Plant root dividing device - Google Patents
Plant root dividing device Download PDFInfo
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- CN210130164U CN210130164U CN201822075631.6U CN201822075631U CN210130164U CN 210130164 U CN210130164 U CN 210130164U CN 201822075631 U CN201822075631 U CN 201822075631U CN 210130164 U CN210130164 U CN 210130164U
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- root
- dividing box
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The utility model discloses a plant divides root device, include: the root dividing box I and an isolation cover II sleeved above the root dividing box I are arranged on the root dividing box I; the root dividing box I is divided into a left root chamber A and a right root chamber B by a middle baffle 5; wherein, the top of the root dividing box I is just opposite to the upper part of the middle baffle plate and is provided with at least 1 seedling containing holes 9. By the aid of the method, consumption of the microorganisms to root exudates, direct influence of microbial metabolites to the root exudates and direct antagonism between the microorganisms can be eliminated, influence of colonization of probiotics/pathogenic bacteria on plant root exudates and influence of change of the root exudates on colonization of the microorganisms can be accurately quantified.
Description
Technical Field
The utility model relates to a microbial cultivation and plant roots detect, concretely relates to research microbial colonization influences plant roots secretion and plant roots secretion changes the plant root device to microbial colonization influence.
Background
The growth of the plants is controlled by microorganisms, and the probiotics or the pathogenic bacteria colonize on the root systems of the plants to induce the plants to secrete different signal substances to influence the colonization of the microorganisms, so that the understanding of the interaction mechanism of the plants, the probiotics and the pathogenic bacteria is helpful for enhancing the disease resistance of the plants and controlling the growth of the plants. With the development of metabonomics, the analysis of plant root secretion is more and more, only the root secretion of a certain plant is collected, and only the plant root system is collected in a container. If microorganisms are colonized, because the microorganisms and the plant root system grow in a space, the consumption of the microorganisms on root system secretion or the direct influence of microorganism metabolites on the root system secretion cannot be eliminated; when the influence of the colonization of probiotics on the colonization of pathogenic bacteria on the plant root system is researched, if the colonization of the pathogenic bacteria is in the same space, whether the colonization of the pathogenic bacteria is under the regulation and control action of root system secretion or the direct antagonism action of the probiotics cannot be accurately quantified.
Therefore, there is a need for a plant rooting device that eliminates the consumption of root exudates by microorganisms or the direct effect of microbial metabolites on root exudates, and facilitates accurate quantification of whether colonization by one microorganism is regulated by a root exudate or directly antagonized by another microorganism.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model provides a plant divides root device to the realization is for accurate quantitative microorganism to the influence of plant roots secretion to and plant roots secretion changes and provides the advantage to the influence of microorganism colonization.
The utility model provides a plant divides root device, include:
the root dividing box I and an isolation cover II sleeved above the root dividing box I are arranged on the root dividing box I;
the root dividing box I is divided into a left root chamber A and a right root chamber B by a middle baffle 5;
wherein, the top of the root dividing box I is just opposite to the upper part of the middle baffle plate and is provided with at least 1 seedling containing holes 9.
By last, the foretell root device of this application can be so that the stem segment of plant is fixed dress seedling hole 9, the root system is separated by intermediate baffle 5 and is got into left side root room A respectively and get into the root system of right side root room B, can test to the root system of different root rooms respectively, for accurate definite quantity microorganism to the influence of plant roots secretion to and plant roots secretion changes and provides the advantage to the influence of microorganism colonization.
Preferably, the root box I is composed of 8 transparent plates, including: a root dividing box left cover 1 above the left root chamber A is fixedly connected with a root dividing box left side plate 3, a root dividing box front side plate 6 and a root dividing box rear side plate 7;
the root dividing box right side plate 4 is fixedly connected with the root dividing box front side plate 6 and the root dividing box rear side plate 7;
the root dividing box I is provided with a root dividing box bottom plate 8 at the bottom, and the root dividing box bottom plate 8 is fixedly connected with the root dividing box front side plate 6, the root dividing box rear side plate 7, the root dividing box left side plate 3 and the root dividing box right side plate 4;
the root dividing box right cover 2 above the right root chamber B is hinged with the root dividing box right side plate 4;
wherein the root box left cover 1 is abutted with the root box right cover 2;
wherein, the top of the middle baffle 5 and the top of the root dividing box I are arranged with a designated distance.
From above, by opening the root box right lid 2, different liquids for experiments can be added to the left root chamber a and the right root chamber B, respectively. For example, sterile MS nutrient solution is added to the left root chamber A, and a bacterial solution (microbial liquid) in logarithmic growth phase is added to the left root chamber A, and an equal amount of sterile water is added to the right root chamber B. Wherein, the right cover 2 of the root-dividing box and the right side plate 4 of the root-dividing box can be connected by transparent adhesive tape.
Preferably, the seedling containing hole 9 is composed of semicircular holes symmetrically arranged at the abutting positions of the root dividing box left cover 1 and the root dividing box right cover 2.
By last, the plant can be through opening root-dividing box right lid 2, and the stem segment is fixed dress seedling hole 9, the root system divides into two parts, gets into left side root chamber A and right side root chamber B respectively.
Preferably, a water outlet 10 is arranged on the front glass plate 6 near the bottom plate 8.
From above, the liquid in the root separating box can be discharged or collected through the water outlet.
Preferably, the aperture of each of the seedling containing hole 9 and the water outlet 10 is 1.2 cm.
From above, the diameter of the seedling containing hole 9 of the above hole is beneficial to the support of the plant stem section. The water outlet 10 with the aperture is beneficial to the drainage or collection of liquid.
Preferably, the root dividing device is made of a material capable of being sterilized at high temperature and high pressure; the transparent plate is made of common glass or organic glass.
Therefore, the root cutting device is not deformed when the root cutting device is sterilized at high temperature.
Preferably, a rubber strip is arranged at the part of the bottom of the isolation cover II, which is contacted with the root separating box I.
By last, cage II's bottom can set up the rubber strip, when the contact between cage II and root box I, can provide the buffering, and can improve the leakproofness simultaneously.
Preferably, the root dividing device further comprises: a plug matching the outlet aperture; and the support ring is used for being arranged at the seedling containing hole 9.
Thus, the outlet can be plugged with a plug when liquid collection is not required. If the plants are thin, the support ring plant stem segments can be used for fixing and supporting the plants in the seedling containing holes. Or the stem section of the plant can be wrapped by sterile cotton to fix and support the plant. Wherein, the support ring can be after two halves ring hinges, and the opening part can be connected through the joint mode.
Preferably, the length, the width and the height of the root dividing box I are respectively 25.6cm, 12cm and 15 cm; the length, the width and the height of the isolation cover II are respectively 26.8cm, 12cm and 25 cm.
From the above, the above dimensions are suitable for experiments in which plants are subjected to root systems.
Preferably, the length, the width and the thickness of the left root box side plate 3 and the right root box side plate 4 are respectively 25.6cm, 10cm and 1 cm; the length, the width and the thickness of the middle baffle 5 are respectively 25.6cm, 8cm and 0.5 cm; the length, the width and the thickness of the root dividing box left cover 1 and the root dividing box right cover 2 are respectively 25.6cm, 7cm and 0.5 cm; the length, the width and the thickness of the root separating box bottom plate 8 are respectively 25.6cm, 10cm and 0.5 cm;
the length, the width and the thickness of the left side plate 11 and the right side plate 12 of the isolation cover are respectively 26.8cm, 25cm and 0.5 cm; the length, the width and the thickness of the upper side plate 13 of the isolation cover are respectively 26.8cm, 12cm and 0.5 cm; the length, width and thickness of the front glass plate 14 and the rear glass plate 15 of the isolation hood are 12cm, 25cm and 0.5 cm.
From the above, the above dimensions and thicknesses are suitable for experiments in which plants are subjected to root systems.
To sum up, this application provides a plant divides root device, divides root device to fix so that the plant stem section is in dress seedling hole 9, the root system is separated by intermediate baffle 5 and is got into left side root room A respectively and get into right side root room B's root system, can test respectively to the root system in different root rooms (be divided into two with the root system), for the influence of accurate definite quantity microorganism to plant roots secretion to and plant roots secretion changes and provides the advantage to the influence of microorganism colonization.
Drawings
Fig. 1 is a schematic view of the overall structure of the plant root-dividing device provided by the present invention;
fig. 2 is a schematic view of a root separating box I and a shielding case II of the plant root separating device provided by the present invention, which are separately shown;
fig. 3 is a front view of the plant root-dividing device provided by the present invention (the surface where the water outlet is located is the front surface);
fig. 4 is a top view of the plant root-dividing device provided by the present invention;
fig. 5 is a side view of the plant root-dividing device provided by the present invention.
Detailed Description
Further areas of applicability of the present invention will become apparent from the detailed description provided hereinafter. It should be understood that the following detailed description and specific examples, while indicating exemplary embodiments of the load bearing assembly, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
Unless defined otherwise, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. It will be understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and the present disclosure and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.
The utility model provides a plant divides root device, include:
the root dividing box I and an isolation cover II sleeved above the root dividing box I are arranged on the root dividing box I; the isolation cover can be directly clamped on the root separating box to form a closed space. The root dividing box I is divided into a left root chamber A and a right root chamber B by a middle baffle 5; wherein, the top of the root dividing box I is just opposite to the upper part of the middle baffle plate and is provided with at least 1 seedling containing holes 9. Foretell root device can make plant j stem section fix dress seedling hole 9, the root system is separated by intermediate baffle 5 and is got into left side root room A respectively and get into the root system of right side root room B, can test to the root system in different root rooms respectively, for accurate definite quantity microorganism to the influence of plant roots secretion to and plant roots secretion changes and provides the advantage to the influence of microorganism colonization.
Specifically, the root box I is composed of 8 transparent plates, including: a root dividing box left cover 1 above the left root chamber A is fixedly connected with a root dividing box left side plate 3, a root dividing box front side plate 6 and a root dividing box rear side plate 7; the root dividing box right side plate 4 is fixedly connected with the root dividing box front side plate 6 and the root dividing box rear side plate 7; the root dividing box I is provided with a root dividing box bottom plate 8 at the bottom, and the root dividing box bottom plate 8 is fixedly connected with the root dividing box front side plate 6, the root dividing box rear side plate 7, the root dividing box left side plate 3 and the root dividing box right side plate 4; wherein, the root box right cover 2 above the right root chamber B is hinged with the root box right side plate 4 (wherein,the right cover 2 of the root-dividing box is connected with the right side plate 4 of the root-dividing box in a mode of being connected with the two through transparent adhesive tapes); wherein the root box left cover 1 is abutted with the root box right cover 2; wherein, the top of the middle baffle 5 and the top of the root dividing box I are arranged with a designated distance. Thus, by opening the root box right lid 2, different experimental liquids can be added to the left root chamber a and the right root chamber B, respectively. For example, sterile MS nutrient solution is added to the left root chamber A, and a bacterial solution (microbial liquid) in logarithmic growth phase is added to the left root chamber A, and an equal amount of sterile water is added to the right root chamber B。
Wherein, dress seedling hole 9 by the symmetry set up in divide root box left side lid 1 with divide the semicircle orifice of root box right side lid 2 butt department and constitute.
Wherein, a water outlet 10 is arranged on the front glass plate 6 near the bottom plate 8. The liquid in the root separating box can be discharged or collected through a water outlet.
Wherein the aperture of the seedling containing hole 9 and the aperture of the water outlet 10 are both 1.2 cm. The diameter of the seedling containing hole 9 of the hole is beneficial to supporting the plant stem section. The water outlet 10 with the aperture is beneficial to the drainage or collection of liquid.
Wherein, the root dividing device is made of a material which can be sterilized at high temperature and high pressure; the transparent plate is made of common glass or organic glass. The root cutting device is not deformed when the root cutting device is sterilized at high temperature.
And a rubber strip is arranged at the part of the bottom of the isolation cover II, which is contacted with the root dividing box I. Through setting up the rubber strip, when the contact between cage II and the root box I, can provide the buffering, and can improve the leakproofness simultaneously.
Wherein, the root device still includes: a plug matching the outlet aperture; the support ring is arranged at the seedling containing hole (9). The outlet can be plugged with a plug when liquid collection is not required. If the plants are thin, the support ring plant stem segments can be used for fixing and supporting the plants in the seedling containing holes. Or the stem section of the plant can be wrapped by sterile cotton to fix and support the plant. The support ring can be a support ring which is hinged by two halves and connected at the opening in a clamping mode.
Wherein the root dividing box I is 25.6cm, 12cm and 15cm in length, width and height respectively; the length, the width and the height of the isolation cover II are respectively 26.8cm, 12cm and 25 cm. The above size comparison is suitable for experiments in which plants undergo root systems.
The root dividing box left side plate 3 and the root dividing box right side plate 4 are respectively 25.6cm, 10cm and 1cm in length, width and thickness; the length, the width and the thickness of the middle baffle 5 are respectively 25.6cm, 8cm and 0.5 cm; the length, the width and the thickness of the root dividing box left cover 1 and the root dividing box right cover 2 are respectively 25.6cm, 7cm and 0.5 cm; the length, the width and the thickness of the root separating box bottom plate 8 are respectively 25.6cm, 10cm and 0.5 cm; the length, the width and the thickness of the left side plate 11 and the right side plate 12 of the root separating box are respectively 26.8cm, 25cm and 0.5 cm;
the length, the width and the thickness of the upper side plate 13 of the isolation cover are respectively 26.8cm, 12cm and 0.5 cm; the length, width and thickness of the front glass plate 14 and the rear glass plate 15 of the isolation hood are 12cm, 25cm and 0.5 cm.
To sum up, this application provides a plant divides root device, divides root device to fix so that the plant stem section is in dress seedling hole 9, the root system is separated by intermediate baffle 5 and is got into left side root room A respectively and get into right side root room B's root system, can test respectively to the root system in different root rooms (be divided into two with the root system), for the influence of accurate definite quantity microorganism to plant roots secretion to and plant roots secretion changes and provides the advantage to the influence of microorganism colonization.
In order to more clearly illustrate the technical solution of the present application, the application of the root-dividing device of the present application is now described by the following implementation:
1. base material preparation
1) The root-dividing device of the present application is prepared.
2) Preparing plants: cucumber; strain: bacillus amyloliquefaciens.
3) Preparing a culture solution: 1/2MS nutrient solution.
2. Test procedure
1) Soaking the cucumber seeds in water for 2-3 hours until the cucumber seeds are full of water. Removing floating seeds, sterilizing with 75% alcohol for 30s, washing with sterile water for 3 times, soaking with 2% sodium hypochlorite for 15min, and washing with sterile water for 6 times. And (3) placing the seeds with the sterilized surfaces into a tissue culture bottle containing vermiculite and sterilized at high temperature and high pressure for germination acceleration culture, after culturing for 4 days in an artificial climate chamber, transferring the cucumber seedlings into a triangular flask containing sterile MS nutrient solution for continuous culture after 4 days, changing the nutrient solution every two days, and continuously culturing until the root system is developed.
2) Sealing the plant root dividing device, sterilizing at 121 deg.C for 30min, placing in a clean bench, and taking off the isolation cover II; opening the right cover 2 of the root separating box; sterile MS nutrient solution is added into the left root chamber A, the right root chamber B is added with the same amount of sterile water, and bacteria solution (microorganism) in logarithmic phase is added into the left root chamber A. The stem section of the aseptic seedling with developed root system is fixed in the seedling-loading hole 9, the root system is divided into root systems respectively positioned in a left root chamber A and a right root chamber B by the middle baffle 5 of the root-dividing box, and the right cover 2 of the root-dividing box is closed. If the plant is thin, the plant stem section can be wrapped by a support ring or sterile cotton in the seedling containing hole to fix and support the plant. The isolation hood is covered above the root-dividing box, and the whole root-dividing device is placed in a phytotron. After the designated culture time, collecting root system secretion on the right side, discharging or collecting liquid in the root dividing box through a water outlet, opening a right cover of the root dividing box, adding liquid on two sides, and continuously collecting.
To sum up, this application provides a plant divides root device, divides root device to fix so that the plant stem section is in dress seedling hole 9, the root system is separated by intermediate baffle 5 and is got into left side root room A respectively and get into right side root room B's root system, can test respectively to the root system in different root rooms (be divided into two with the root system), for the influence of accurate definite quantity microorganism to plant roots secretion to and plant roots secretion changes and provides the advantage to the influence of microorganism colonization.
The utility model discloses do not confine the aforesaid to concrete operation, the all is in the utility model discloses the design is started, and any modification, simple improvement and equivalence replacement etc. of doing are all in the utility model discloses a protection within range.
Claims (10)
1. A plant root-splitting device comprising:
the root dividing box (I) and an isolation cover (II) sleeved above the root dividing box (I);
wherein the root dividing box (I) is divided into a left root chamber (A) and a right root chamber (B) by a middle baffle plate (5);
wherein, the top of the root separating box (I) is just opposite to the upper part of the middle baffle plate and is provided with at least 1 seedling containing holes (9).
2. The plant rooting-out device according to claim 1, wherein the rooting box (I) consists of 8 transparent plates comprising: a root dividing box left cover (1) above the left root chamber (A) is fixedly connected with a root dividing box left side plate (3), a root dividing box front side plate (6) and a root dividing box rear side plate (7); a root dividing box right cover (2) and a root dividing box right side plate (4) above the right root chamber (B); a root dividing box bottom plate (8) arranged at the bottom of the root dividing box (I); and a middle baffle (5) arranged on the root-dividing box bottom plate (8);
the root dividing box right side plate (4) is fixedly connected with the root dividing box front side plate (6) and the root dividing box rear side plate (7);
the root dividing box (I) is provided with a root dividing box bottom plate (8) at the bottom, and the root dividing box bottom plate is fixedly connected with the root dividing box front side plate (6), the root dividing box rear side plate (7), the root dividing box left side plate (3) and the root dividing box right side plate (4);
wherein, a root dividing box right cover (2) above the right root chamber (B) is hinged with a root dividing box right side plate (4);
wherein the root-dividing box left cover (1) is abutted with the root-dividing box right cover (2);
wherein, the top of the middle baffle (5) and the top of the root dividing box (I) are arranged with a designated distance.
3. The plant rooting-splitting device according to claim 2, wherein the seedling-holding holes (9) consist of semicircular holes symmetrically arranged at the abutting positions of the left rooting-splitting box cover (1) and the right rooting-splitting box cover (2).
4. The plant rooting device according to claim 3, wherein the front panel (6) of the rooting box is provided with a water outlet (10) close to the bottom panel (8).
5. The plant rooting-out device according to claim 4, wherein the hole diameters of the seedling containing hole (9) and the water outlet (10) are both 1.2 cm.
6. The plant rooting apparatus according to claim 2,
the root dividing device is made of a material capable of being sterilized at high temperature and high pressure;
the transparent plate is made of common glass or organic glass.
7. The root segmentation device of claim 1, wherein: and a rubber strip is arranged at the part of the bottom of the isolation cover (II) which is contacted with the root dividing box (I).
8. The root segmentation device of claim 4, further comprising:
a plug matching the outlet aperture;
the support ring is arranged at the seedling containing hole (9).
9. The root segmentation device of claim 2,
the length, the width and the height of the root separating box (I) are respectively 25.6cm, 12cm and 15 cm;
the length, the width and the height of the isolation cover (II) are respectively 26.8cm, 12cm and 25 cm.
10. The root segmentation device according to any one of claims 2-6,8-9,
the length, the width and the thickness of the root dividing box left side plate (3) and the root dividing box right side plate (4) are respectively 25.6cm, 10cm and 1 cm;
the length, the width and the thickness of the middle baffle (5) are respectively 25.6cm, 8cm and 0.5 cm;
the length, the width and the thickness of the root dividing box left cover (1) and the root dividing box right cover (2) are respectively 25.6cm, 7cm and 0.5 cm; the length, the width and the thickness of the root separating box bottom plate (8) are respectively 25.6cm, 10cm and 0.5 cm;
the length, the width and the thickness of a left side plate (11) and a right side plate (12) of the isolation cover are respectively 26.8cm, 25cm and 0.5 cm;
the length, the width and the thickness of an upper side plate (13) of the isolation cover are respectively 26.8cm, 12cm and 0.5 cm;
the length, width and thickness of the front glass plate (14) and the rear glass plate (15) of the isolation cover are 12cm, 25cm and 0.5 cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822075631.6U CN210130164U (en) | 2018-12-11 | 2018-12-11 | Plant root dividing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822075631.6U CN210130164U (en) | 2018-12-11 | 2018-12-11 | Plant root dividing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210130164U true CN210130164U (en) | 2020-03-10 |
Family
ID=69701863
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201822075631.6U Expired - Fee Related CN210130164U (en) | 2018-12-11 | 2018-12-11 | Plant root dividing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210130164U (en) |
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2018
- 2018-12-11 CN CN201822075631.6U patent/CN210130164U/en not_active Expired - Fee Related
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Granted publication date: 20200310 Termination date: 20201211 |