CN106701654A - Regular morphology of fusarium oxysporum laboratory material and preparation and application method thereof - Google Patents
Regular morphology of fusarium oxysporum laboratory material and preparation and application method thereof Download PDFInfo
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- CN106701654A CN106701654A CN201710034044.XA CN201710034044A CN106701654A CN 106701654 A CN106701654 A CN 106701654A CN 201710034044 A CN201710034044 A CN 201710034044A CN 106701654 A CN106701654 A CN 106701654A
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- spore
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- banana blight
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N3/00—Spore forming or isolating processes
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Abstract
The invention discloses a regular morphology of a fusarium oxysporum laboratory material and a preparation and application method thereof. Fusarium oxysporum pure and pollution-free conidiospore is used as a regular morphology of the laboratory material. The conidiospore serving as the regular morphology is stored in sterile water. The preparation and application method comprises the following steps that 1, a triangular flask serves as a culture container to prepare the fusarium oxysporum conidiospore; 2, the regular morphology of the fusarium oxysporum material is prepared; 3, the conidiospore content of the regular morphology of the fusarium oxysporum material is calibrated; 4, the regular morphology of the fusarium oxysporum material is daily stored; 5, the regular morphology of the fusarium oxysporum material is applied. The regular morphology has the characteristics and advantages that the conidiospore serving as the regular morphology can suspend to grow in pure water and can be instantly restored to normal growth when being transferred to a culture medium; the regular morphology can be applied in relevant work at any time, a thallus material can be directly provided for a test link needing the conidiospore, the working process needing to repeatedly prepare and culture the conidiospore when a mycelium is used as a commonly prepared material can be omitted, and work is convenient and quick; 3, the triangular flask serves as the culture container, and pure and pollution-free conidiospore liquid is easy to prepare; the conidiospore content of the commonly prepared conidiospore liquid can be calibrated and can be accurately quantified and taken in application, and standardization of a technical method is promoted.
Description
Technical field
The present invention relates to Plant Pathology technology.The standing form of specifically a kind of banana blight bacteria lab material and
Its preparation and application method.
Background technology
Banana blight is the great disease on banana, can cause destructive heavy losses to banana production, the disease
Infected and caused by banana blight bacteria (Fusarium oxysporum).Banana blight bacteria is easily given birth on ordinary culture medium
Long, mycelium is flourishing；In laboratory work, banana blight bacteria is generally trained inclined-plane bacterium with test tube slant culture medium
Fall, and using the mycelium material of bacterium colony as the standing material of routine work.About research work starting point with mycelium
Material is starting, by transplanting standing material mycelium, is enlarged culture or enters the research work of detailed programs.It is this with
Mycelium as routine work material standing form, it is generally more simple and convenient, but there is also that some are not enough, it is as follows：
Relevant research work is determined with growth rate (or increment), (influence factor such as grown is determined, medicine
Inhibitory action measure of thing etc.) in, mycelial quantitative transplanting is generally required, and the mycelium transplanted in culture medium is difficult to do
To accurate quantitative analysis, conventional method so far be need first to transplant mycelium to culture plate Amplification Culture, then use card punch
The mycelia block for taking equivalent diameter is beaten on the flat-plate bacterial colony of Amplification Culture, quantitative transplanting is carried out with this mycelia block；This quantitative square
Formula will not only increase by one section of program link of Amplification Culture, and because the thickness of culture plate is inconsistent, and bacterium colony is different
The mycelial dense degree in position, physiological status etc. are also variant, cause to be difficult between different mycelia blocks to reach higher consistent
Property.
Many research work (such as the influence factor of spore germination, disease inoculation etc.), the direct material for using is typically point
Raw spore, is equally transplanted to suitable culture medium by standing material (mycelium), and leading spore prepares training, obtains spore
Related work could be implemented after son.This needs to delay equivalent to related work process, it is impossible to carry out in time.
The content of the invention
Standing form and its preparation and application it is an object of the invention to provide a kind of banana blight bacteria lab material
Method.
The technical scheme that the present invention solves above-mentioned technical problem is as follows：
The standing form of banana blight bacteria lab material, is to make routine work with the conidium of banana blight bacteria
Standing form；Conidium is pure pollution-free；Conidium is deposited in sterilized water；Conidium pause is grown, but
Go to the development that can be restore normal growth at once on culture medium.
The preparation and application method of the standing form of banana blight bacteria lab material, preparation and application method and step is such as
Under：
1. it is conidial to prepare culture
Make culture utensil with triangular flask, contain potato dextrose medium and make product spore culture medium, the component of the culture medium
Match and be：Potato 200g, glucose 20g, agar 20g, water 1000mL；The mycelium of banana blight bacteria is implanted into triangle
In product spore culture medium in bottle, cultivate at a suitable temperature, until forming bacterium colony and conidium on culture basal plane.
2. the preparation of the standing form of banana blight bacteria material
Take on the product spore bacterium colony that the culture of sterilized water implantation step 1 is obtained, gently elute the spore on bacterium colony, acquisition contains bacterium
The spore liquid of broken section of silk；Broken section of the mycelia removed in the spore liquid is filtered with sterile gauze；The spore liquid is carried out into centrifugation,
Supernatant is outwelled, the bacterial metabolism product and nutrient media components mixed in spore liquid is removed；It is remaining be precipitated as it is pure mitogenetic
Spore；Using the pure conidium as the standing form of banana blight bacteria material；Spore suspension will be precipitated with sterilized water, will be stirred
It is dynamic to make spore fully dispersed, obtain the standing spore liquid of banana blight bacteria material.
3. the spore content of the standing form of banana blight bacteria material is demarcated
The spore concentration of the standing spore liquid for being obtained step 2 with sterilized water, is allocated as 106Spore/mL.
4. the daily storage of the standing form of banana blight bacteria material
Aseptically, the standing spore liquid that step 3 is demarcated is dispensed into the closed sterilization container of lid, and is shifted
Deposit standby in 25 DEG C of dark situations.
5. the application of the standing form of banana blight bacteria material
The standing spore liquid of banana blight bacteria material that step 4 is deposited goes to superclean bench, and vibration dress deposits standing spore
The container of sub- liquid, makes conidium fully suspend and disperse；Spore liquid is quantitatively drawn with pipettor after uncapping, correlation is transplanted to
In working link or experimental arrangement；Imbibition graft procedure returns lid sealing after finishing, remaining standing spore liquid is gone back into step 4
Deposit storeroom.
Characteristic of the invention is with advantage：
1) conidium of standing form can suspend growth in pure water, but go to culture medium, can at once recover normal life
It is long.
2) can at any time be applied in related work, can directly provide thalline material for the conidial experiment link of needs,
, it is necessary to prepare the working procedure of culture spore repeatedly when can eliminate the need for mycelium as standing material, work convenient and swift.
3) make culture utensil with triangular flask, be readily available free of contamination pure spore liquid, the standing form matter for preparing
Amount is higher.
4) spore content of standing spore liquid can be demarcated, using when can be taken with accurate quantitative analysis, beneficial to technical method standard
Change.
Specific embodiment
With reference to embodiment, the invention will be further described.
In the relevant research of banana blight, standing form is generally made using the mycelium material of banana blight bacteria,
Every time when needing conidium material, it is intended to transplant standing mycelium to new culture medium and Amplification Culture and prepares culture spore
Son.Currently yet there are no technological thought and the technology application for making standing transplant using conidium.Inventor is in research work
The middle conidium for finding banana blight bacteria can suspend in pure water and grow, but goes to and can recover at once just on culture medium
It is frequently grown development；Using this favourable biological characteristics, the another kind of present invention design banana blight bacteria lab material
Standing form, exactly makees standing form with the conidium material of banana blight bacteria, and the standing form preparation with should
Use technology.
The substantial amounts of conidium finished product of banana blight bacteria, but the party can be generally prepared with agitated submerged culture method
Method needs shaken cultivation equipment, and the conidium finished product for obtaining contain more medium component, bacterial metabolism product and
Mycelium, thus common laboratory also commonly use solid plate cultivation prepare banana blight bacteria spore.
Solid plate cultivation used by current experiment room is to make culture utensil using culture dish, but due to the knot of culture dish
Structure defect determines that the air exchange inside and outside culture dish is smooth, is easily caused the biomaterial pollution in culture dish.Invention human hair
In the triangular flask of present relative closure, banana blight bacteria also can normally produce spore, and triangular flask prevents the effect of pollution very good,
Thus making culture utensil using triangular flask carries out producing spore culture.
Banana blight bacteria can be grown on many culture mediums and form conidium, and the present invention is commonly used using laboratory
Potato dextrose medium, its proportioning is：Potato 200g, glucose 20g, agar 20g, water 1000mL.Banana blight
Bacterium is cultivated 5~7 days on the potato dextrose medium that triangular flask is contained under conditions of being 28 DEG C in temperature, can be in culture
The bacterium colony of big volume production spore is formed and is covered with basal plane.
The conidium produced on spore bacterium colony can be collected with aseptic water washing, gently smeared with instruments such as glass rod or small brushes and washed bacterium
Falling to spore washing can be just discharged into sterilized water.
The washing operation for collecting spore can cause nutrient media components and the mycelial excretion metabolin of bacterium colony to be dissolved in spore
In liquid, these materials may be easily caused growth or the inactivation of spore, so that removal；Centrifugation spore mode can be used
Remove these materials.
Used as the spore liquid of the standing form of banana blight bacteria material, spore concentration can be high or low, it is contemplated that excessive concentrations
The activity of spore may be influenceed, and is matched with the need for subsequent applications, the spore concentration of standing spore liquid is adjusted 106
Spore/mL is more suitable, and the quantity of spore is determined with blood counting chamber.
Dress deposits the utensil that the container of the standing spore liquid of banana blight bacteria material should be sealed and close using that can sterilize and can cover, such as
General freezer pipe or centrifuge tube etc., are filled with 5mL cryovials and deposit more practical and convenient；Need dress storage more, triangular flask can be used
Or big cryovial (or big centrifuge tube).
Storage environment is advisable with room temperature, and inventor has found good in the dark situation storage effect that temperature is 25 DEG C.
Using before the standing spore liquid of banana blight bacteria, it is necessary to by standing spore liquid vibrate, make settled spore again
Suspend and be uniformly dispersed.Standing spore liquid aseptically is taken out with pipettor, the experiment work ring of correlation is can be directly used for
Section, such as artificial infection of the flat board culture, Liquid Culture or banana blight of banana blight bacteria.In standing spore liquid
Spore good dispersion, the uniformity consistency of thalline liquid is high, adds the easy accurate quantitative analysis of liquid and draws, thus, it is of the invention standing
Spore liquid is particularly conducive to need the training of quantitative transplanting thalline, such as determine influence that banana blight bacteria grows because
Element, determines inhibitory action of medicine etc..
Cause the probability of standing form spore liquid pollution to reduce experimental implementation, the reduction that should try one's best deposits standing spore liquid from dress
Container in draw the frequency of liquid repeatedly, it is disposable all to draw enough whole examinations the need for real work can be by experimental design
The spore liquid measure tested, is placed in other sterilization container, then quantitatively draws spore liquid one by one from the container, is transplanted to each
Culture plate, triangular flask culture medium or each host's inoculation position, etc. in related working link or experimental arrangement.
Embodiment 1
Using the standing form and its preparation and application method of banana blight bacteria lab material of the present invention, prepared by culture
Obtain laboratory standing form materials of the banana blight bacteria strain Fo-1 with conidium as thalline；Preparation and application bacterial strain
The standing form of Fo-1 materials implements operation as follows：
1. it is conidial to prepare culture
Make culture utensil with triangular flask, contain potato dextrose medium and make product spore culture medium, the component of the culture medium
Match and be：Potato 200g, glucose 20g, agar 20g, water 1000mL；The mycelium of banana blight bacteria strain Fo-1 is moved
In product spore culture medium in implantation triangular flask, after being cultivated 5 days at a temperature of 28 DEG C, the bacterium colony on culture basal plane forms substantial amounts of point
Raw spore.
2. the preparation of the standing form of banana blight bacteria material
Take on the product spore bacterium colony that the culture of sterilized water implantation step 1 is obtained, gently elute the spore on bacterium colony, acquisition contains bacterium
The spore liquid of broken section of silk；Broken section of the mycelia removed in the spore liquid is filtered with sterile gauze；The spore liquid is carried out into centrifugation,
Supernatant is outwelled, the bacterial metabolism product and nutrient media components mixed in spore liquid is removed；It is remaining be precipitated as it is pure mitogenetic
Spore；Using the pure conidium as the standing form of banana blight bacteria material；Spore suspension will be precipitated with sterilized water, will be stirred
It is dynamic to make spore fully dispersed, obtain the standing spore liquid of banana blight bacteria strain Fo-1.
3. the spore content of the standing form of banana blight bacteria material is demarcated
The spore concentration of the standing spore liquid for being obtained step 2 with sterilized water, is allocated as 106Spore/mL.
4. the daily storage of the standing form of banana blight bacteria material
Aseptically, the standing spore liquid that step 3 is demarcated is dispensed into 5mL cryovials, and is transferred to 25 DEG C
Deposited in dark situation standby.
5. the application of the standing form of banana blight bacteria material
The standing spore liquid of banana blight bacteria material that step 4 is deposited goes to superclean bench, and vibration dress deposits standing spore
The container of sub- liquid, makes conidium fully suspend and disperse；Spore liquid is drawn after uncapping with pipettor to use；Imbibition end of operation
After return lid sealing, by remaining standing spore liquid go back to step 4 storeroom storage.
The standing spore liquid 5 μ L are quantitatively drawn with pipettor, potato glucose culture plate middle part, 28 DEG C of trainings is transplanted to
After supporting 2 days, a typical petite can be grown into.
The standing spore spore liquid that 10 μ L have been demarcated is drawn, potato dextrose broth is transplanted to, shaken at a temperature of 28 DEG C
A large amount of a new generation conidiums are formed after swinging culture 5 days, in nutrient solution.
Embodiment 2
Using the standing form and its preparation and application method of banana blight bacteria lab material of the present invention, prepared by culture
Obtain laboratory standing form materials of the banana blight bacteria strain Fo-2 with conidium as thalline；Preparation and application bacterial strain
The step of standing form of Fo-2 materials presses embodiment 11 implements to operate to step 5, and the different bacterial strains simply used by step 1 are
Fo-2, step 2 obtains the standing spore liquid of banana blight bacteria strain Fo-2.
The μ L of standing spore liquid 5 for having demarcated quantitatively are drawn with pipettor, potato glucose culture plate middle part is transplanted to,
After 28 DEG C are cultivated 2 days, a typical petite can be grown into.
The standing spore spore liquid that 10 μ L have been demarcated is drawn, potato dextrose broth is transplanted to, shaken at a temperature of 28 DEG C
A large amount of a new generation conidiums are formed after swinging culture 5 days, in nutrient solution.
Claims (2)
1. the standing form of banana blight bacteria lab material, it is characterised in that made with the conidium of banana blight bacteria
The standing form of routine work；Conidium is pure pollution-free；Conidium is deposited in sterilized water；Conidium pause growth
Development, but go to the development that can be restore normal growth at once on culture medium.
2. the preparation and application method of the standing form of banana blight bacteria lab material as claimed in claim 1, it is special
Levy and be, methods for making and using same step is as follows：
1) it is conidial to prepare culture：Make culture utensil with triangular flask, contain potato dextrose medium and make to produce spore culture
Base, the component proportion of the culture medium is：Potato 200g, glucose 20g, agar 20g, water 1000mL；By banana blight bacteria
Mycelium be implanted into the product spore culture medium in triangular flask, cultivate at a suitable temperature, until culture basal plane on form bacterium
Fall and conidium；
2) preparation of the standing form of banana blight bacteria material：Take sterilized water implantation step 1) cultivate on the product spore bacterium colony for obtaining,
Gently the spore on wash-out bacterium colony, obtains the spore liquid containing broken section of mycelia；With sterile gauze filter remove the spore liquid in
Broken section of mycelia；The spore liquid is carried out into centrifugation, supernatant is outwelled, the bacterial metabolism product and training mixed in spore liquid is removed
Support base component；It is remaining to be precipitated as pure conidium；Using the pure conidium as the normal of banana blight bacteria material
Standby form；Spore suspension will be precipitated with sterilized water, agitation makes spore fully dispersed, obtains the standing spore of banana blight bacteria material
Sub- liquid；
3) spore content of the standing form of banana blight bacteria material is demarcated：With sterilized water by step 2) the standing spore liquid that obtains
Spore concentration, be allocated as 106Spore/mL；
4) the daily storage of the standing form of banana blight bacteria material：Aseptically, by step 3) demarcate standing spore
Liquid is dispensed into the closed sterilization container of lid, and is transferred in 25 DEG C of dark situation and is deposited standby；
5) application of the standing form of banana blight bacteria material：By step 4) storage the standing spore liquid of banana blight bacteria material
Superclean bench is gone to, vibration dress deposits the container of standing spore liquid, conidium is fully suspended and is disperseed；Liquid relief is used after uncapping
Device quantitatively draws spore liquid, is transplanted in the working link of correlation or experimental arrangement；Imbibition graft procedure returns lid sealing after finishing,
Remaining standing spore liquid is gone back into step 4) storeroom storage.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107641599A (en) * | 2017-09-29 | 2018-01-30 | 华南农业大学 | A kind of banana blight bacterium culture medium and its application |
| CN113308512A (en) * | 2021-06-15 | 2021-08-27 | 广西大学 | Dry transplanting method for conidia of banana fusarium wilt |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103436448A (en) * | 2013-08-20 | 2013-12-11 | 广西大学 | Method for preserving strain of ustilaginoidea virens for long time |
| WO2014086850A1 (en) * | 2012-12-04 | 2014-06-12 | Basf Agro B.V., Arnhem (Nl) | Compositions comprising a quillay extract and a fungicidal inhibitor of respiratory complex ii |
| CN104277977A (en) * | 2013-07-01 | 2015-01-14 | 中粮生物化学（安徽）股份有限公司 | Preparation method for aspergillus niger spore suspension liquid and storage method for aspergillus niger spore |
| CN105861412A (en) * | 2016-05-24 | 2016-08-17 | 广西大学 | Culture and preparation method for pyricularia oryza conidia |
| CN105950699A (en) * | 2016-07-06 | 2016-09-21 | 广东海洋大学 | Method for identifying pathogenicity of pathogenic bacteria of banana wilt |
-
2017
- 2017-01-10 CN CN201710034044.XA patent/CN106701654A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014086850A1 (en) * | 2012-12-04 | 2014-06-12 | Basf Agro B.V., Arnhem (Nl) | Compositions comprising a quillay extract and a fungicidal inhibitor of respiratory complex ii |
| CN104277977A (en) * | 2013-07-01 | 2015-01-14 | 中粮生物化学（安徽）股份有限公司 | Preparation method for aspergillus niger spore suspension liquid and storage method for aspergillus niger spore |
| CN103436448A (en) * | 2013-08-20 | 2013-12-11 | 广西大学 | Method for preserving strain of ustilaginoidea virens for long time |
| CN105861412A (en) * | 2016-05-24 | 2016-08-17 | 广西大学 | Culture and preparation method for pyricularia oryza conidia |
| CN105950699A (en) * | 2016-07-06 | 2016-09-21 | 广东海洋大学 | Method for identifying pathogenicity of pathogenic bacteria of banana wilt |
Non-Patent Citations (3)
| Title |
|---|
| 林吉恒等: "黑曲霉孢子悬液贮存期的验证", 《中国药品标准》 * |
| 谢俊等: "香蕉枯萎病菌的分离鉴定与形态学研究", 《热带生物学报》 * |
| 魏岳荣等: "香蕉感染枯萎病菌4号小种后的生理变化", 《2008园艺学进展（第八辑）——中国园艺学会第八届青年学术讨论会暨现代园艺论坛论文集》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107641599A (en) * | 2017-09-29 | 2018-01-30 | 华南农业大学 | A kind of banana blight bacterium culture medium and its application |
| CN107641599B (en) * | 2017-09-29 | 2021-04-23 | 华南农业大学 | Banana fusarium wilt bacterium culture medium and application thereof |
| CN113308512A (en) * | 2021-06-15 | 2021-08-27 | 广西大学 | Dry transplanting method for conidia of banana fusarium wilt |
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Application publication date: 20170524 |