CN101469311B - Large-sized fungal sporule field timing collecting device - Google Patents
Large-sized fungal sporule field timing collecting device Download PDFInfo
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- CN101469311B CN101469311B CN2007101592923A CN200710159292A CN101469311B CN 101469311 B CN101469311 B CN 101469311B CN 2007101592923 A CN2007101592923 A CN 2007101592923A CN 200710159292 A CN200710159292 A CN 200710159292A CN 101469311 B CN101469311 B CN 101469311B
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Abstract
The invention relates to a field timing acquisition device for large-scale fungal spores, which comprises a bracket and a pointer clock provided with an hour pointer shaft core, wherein the pointer clock is fixed on the bracket; a lower dial plate is fixed at the upper end of the pointer shaft core of the pointer clock and has a disc structure; an upper dial plate is covered above the lower dial plate and has an inverse U-shaped structure of which the peripheral edge is supported on the bracket; and the upper dial plate is provided with a hole corresponding to the lower dial plate. The field timing acquisition device can acquire and preserve fungi in situ and at fixed point and definite time with labor saving, has wide application scope, and can acquire spores released by all the fruiting body type large-scale fungi with all the shapes at fixed point and definite time. A release curve of the fungal spores within 24 hours is conveniently made by utilization of the spores acquired by utilizing a sterile transparent plastic film, so that preservation of single-cell strains, single-cell fusion culture of the type and study on the medical and industrial application of the strains are convenient.
Description
Technical field
The present invention relates to the large-sized fungal sporule field acquisition, specifically a kind of spore that can discharge single macro fungi in the open air carries out the device of timing acquiring.
Background technology
The macro fungi kind that exists in the Forest ecosystems reaches thousands of kinds, and collecting these mycetogenetic spores is the important steps of it being carried out Research on kinds and strain separating preservation.Yet the kind that One's name is legion is wherein arranged, because its physiology and ecological habit also are not familiar with by people, the time of spore development and release and required envrionment conditions are also indeterminate, therefore be difficult to find and collect the spore of its generation usually, this utilizes these valuable resources to cause very big inconvenience for people's research and development.
The kind that One's name is legion is arranged in the macro fungi, particularly some porous classes and lead fungi class fungi, spore development and release a few skies in vegetative period, and also only in a certain short time, discharging in the middle of one day, for many macro fungi kinds with this characteristic, its spore discharges period and the time length is unknown, so people are difficult to find and collect the spore of its generation usually, and this also causes at present for the less present Research of the research in this field.Traditional method is to place a slide glass below the macro fungi sporophore, and each hour changed once in one day, so just can count the density of spore-bearing period of this bacterium and release spore quantity; Yet traditional method consumption power is big, and the shortcoming of length consuming time is apparent.
Summary of the invention
The purpose of this invention is to provide a kind of large-sized fungal sporule field timing collecting device, the purpose that the spore that adopts the present invention macro fungi to be discharged carries out original position, fixed point, timing acquiring and preservation with can realizing saving labor in the open air.
For achieving the above object, the technical solution used in the present invention is:
A kind of large-sized fungal sporule field timing collecting device, comprise carriage and the analog timepiece that has the hour hand axle core, analog timepiece is fixed on the carriage, the hour hand axle core upper end of analog timepiece is fixed with down dial plate, and following dial plate is a disc-shaped structure, and following dial plate top is covered with dial plate, last dial plate is the inverted U structure, the peripheral outer of inverted U structure is supported on the carriage, and the area of promptly going up dial plate is promptly gone up the vertical projection of dial plate can will descend the vertical projection of dial plate to cover fully greater than following dial plate; Offer on corresponding to dial plate on above the following dial plate porose.
That described hole of going up on the dial plate can be is trapezoidal, square, Polygons, ellipse, circle or fan-shaped; If the radius of following dial plate will descend dial plate for the segmental limit and be separated into the identical sector region of several shapes, the vertical projection in hole should be in the sector region of following dial plate disk area 1/6-1/24 size on the so described last dial plate.
Described down dial plate upper berth is provided with film, indicates the fan section of time scale on the film, and it is that the radius of following dial plate will descend dial plate for the segmental limit and be separated into 6,12,24,36 or 48 sector regions that shape is identical.
Offer center positioning hole corresponding to hour hand axle core place on the dial plate on described
The present invention has following advantage:
1. the spore that adopts the present invention to discharge macro fungi in the open air carries out original position collection of fixed place and time and preservation with saving labor.
2. applied widely, can be in the open air to the macro fungi of the sporophore type of nearly all shape, carry out the collection of fixed place and time that spore discharges as agaric, pore fungus, hedgehog fungus and photovoltaicing leather bacteria etc.
3. the spore that utilizes sterile transparent super-thin plastic film to collect carries out the spore density inspection with microscope, makes 24 hours release profiles of this kind fungal spore easily, clear and definite its physiological and ecological characteristic; The spore that collects can be carried out the unit cell separation and Culture, preserve the unit cell bacterial strain, the unit cell fusion of carrying out this kind after being convenient to is cultivated, the medicinal and industrial use research of bacterial classification.
Description of drawings
Fig. 1 is apparatus of the present invention structural representation;
Fig. 2 is an index dial structure synoptic diagram on apparatus of the present invention
Fig. 3 is a dial plate structural representation under apparatus of the present invention.
Drawing reference numeral explanation: 1, go up dial plate, 2, dial plate down, 3, carriage, 4, analog timepiece, 5, the hole, 6, film, 7, the hour hand axle core, 8 centre holes.
Embodiment
As shown in Figure 1, 2, 3, a kind of large-sized fungal sporule field timing collecting device, principle when this device utilizes the clock and watch tropometer adopts double-deck detachable structure; The analog timepiece 4 that it comprises carriage 3 and has hour hand axle core 7, analog timepiece 4 is fixed on the carriage 3, hour hand axle core 7 upper ends of analog timepiece 4 are fixed with down dial plate 2, following dial plate 2 is a disc-shaped structure, following dial plate 2 tops are covered with dial plate 1, and last dial plate 1 is the inverted U structure, and the peripheral outer of inverted U structure is supported and is positioned on the carriage 3, the area of promptly going up dial plate 1 is promptly gone up the vertical projection of dial plate 1 can will descend the vertical projection of dial plate 2 to cover fully greater than following dial plate 2; On corresponding to dial plate 1 on above the following dial plate 2, offer porose 5.
The described circular lightweight dish (present embodiment employing plastic material) of dial plate 1 of going up for ease of opening, diameter is 6～10cm, be preferably 8cm, its surface is pressed the time subregion of dial plate 2 and is opened a scallop hole 5 in 12 positions, is used to block the large-sized fungal sporule big area and launches; If the radius of following dial plate 2 will descend dial plate 2 for the segmental limit and be separated into the identical sector region of several shapes, the vertical projection in hole 5 should be in the sector region of following dial plate 2 disk areas 1/12 size on the so described last dial plate 1;
Described dial plate 2 down is lightweight disk (a present embodiment employing plastic material), diameter is 5～9cm, be preferably 7cm, center fixation is on the hour hand axle core 7 of quartz clock, be equipped with easy-to-dismount sterilization lightweight transparent film 6 (present embodiment adopts super-thin plastic film 6) on it, indicate the fan section of time scale on the film 6, it is that the radius of following dial plate 2 will descend dial plate 2 for the segmental limit and be separated into 12 sector regions that shape is identical, is respectively 12 small time taking districts; Placing film first after 6 12 hours, taking off this film 6, reapposing new film 6 again, continuing 12 hours, 2 films 6 write down 24 hours spore dispose procedure of whole day altogether; Gather by the spore that discharges in 6 pairs of macro fungi whole days of this film 24 hours, after film 6 taken back the laboratory, utilize microscope the spore on the film 6 is checked and to be measured, determine period and quantity that this large-sized fungal sporule discharges, and make the spore release profiles, find out its physiological ecology habit; Simultaneously, the spore that collects can be carried out the unit cell separation and Culture, preserve the unit cell bacterial strain, the unit cell fusion of carrying out this kind after being convenient to is cultivated, the medicinal and industrial use research of bacterial classification.
During use, the last dial plate 1 of device is opened, the plastics film of making 12 small time taking marks 6 disks after the alcohol sterilization of 7 centimetres of diameters are placed down on the dial plate 2, cover dial plate 1, with the active product spore part of place, last dial plate 1 fan-shaped aperture aligning macro fungi sporophore, about 1 centimetre apart from the sporophore lower edge in last dial plate 1 places whole device horizontal fixed, open the clock and watch switch, the slow rotation work of device beginning; (as 6 points morning) simultaneously pick up counting.After 12 hours, promptly late 18 points will install dial plate 1 and open, and carefully take out plastics film 6, be placed in the specific sterilization cassette and take back.New plastics film 6 is placed on down on the dial plate 2, begins other 12 hours collection.Early 6 the time, take out film 6 next day, gets back to indoorly at the microscopically spore in each scale area on 2 films 6 of check measurement respectively, by the counting of equal area Endospore quantity, can obtain the distribution density of spore.After 24 interior spore distribution density measurements of scale area are finished, can make the spore release profiles.Spore can also carry out the unit cell separation and Culture in the microscopically operation simultaneously, the bacterial strain of separation and Culture is preserved, so that carry out follow-up study.
Claims (5)
1. a large-sized fungal sporule field timing collecting device comprises carriage and the analog timepiece that has the hour hand axle core, it is characterized in that:
Analog timepiece is fixed on the carriage, the hour hand axle core upper end of analog timepiece is fixed with down dial plate, following dial plate is a disc-shaped structure, following dial plate top is covered with dial plate, last dial plate is the inverted U structure, the peripheral outer of inverted U structure is supported on the carriage, offers on corresponding to dial plate on above the following dial plate porose.
2. according to the described gathering device of claim 1, it is characterized in that: described hole of going up on the dial plate is trapezoidal, square, Polygons, ellipse, circle or fan-shaped; If the radius of following dial plate will descend dial plate for the segmental limit and be separated into the identical sector region of several shapes, the vertical projection in hole should be in the sector region of following dial plate disk area 1/6-1/24 size on the so described last dial plate.
3. according to the described gathering device of claim 1, it is characterized in that: described dial plate upper berth down is provided with film, indicate the fan section of time scale on the film, it is that the radius of following dial plate will descend dial plate for the segmental limit and be separated into 6,12,24,36 or 48 sector regions that shape is identical.
4. according to the described gathering device of claim 1, it is characterized in that: offer center positioning hole corresponding to hour hand axle core place on the dial plate on described.
5. according to the described gathering device of claim 1, it is characterized in that: the described area of going up dial plate is promptly gone up the vertical projection of dial plate and can will be descended the vertical projection of dial plate to cover fully greater than following dial plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101592923A CN101469311B (en) | 2007-12-28 | 2007-12-28 | Large-sized fungal sporule field timing collecting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007101592923A CN101469311B (en) | 2007-12-28 | 2007-12-28 | Large-sized fungal sporule field timing collecting device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101469311A CN101469311A (en) | 2009-07-01 |
| CN101469311B true CN101469311B (en) | 2011-08-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007101592923A Expired - Fee Related CN101469311B (en) | 2007-12-28 | 2007-12-28 | Large-sized fungal sporule field timing collecting device |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107739729B (en) * | 2017-01-20 | 2023-09-12 | 中国计量大学 | Portable outdoor spore separator and spore separation and collection method |
| CN107560987B (en) * | 2017-09-11 | 2020-03-20 | 重庆交通大学 | Atmospheric biological particle concentration calculation method |
| CN107631914B (en) * | 2017-09-11 | 2020-03-20 | 重庆交通大学 | Biological particle collector |
| CN109957502B (en) * | 2019-05-20 | 2024-03-26 | 山东农业大学 | Portable spore catcher and catching method |
| CN113518528B (en) * | 2021-07-14 | 2023-08-08 | 海南青峰生物科技有限公司 | Intelligent spore capturing and identifying system device and method based on image processing |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2825646Y (en) * | 2005-09-12 | 2006-10-11 | 吉林农业大学 | Timing automatic catching instrument for fungal spore |
| CN1869201A (en) * | 2005-09-26 | 2006-11-29 | 丽水市林业科学研究所 | Collecting method of ganoderma spore powder and powder collecting device |
-
2007
- 2007-12-28 CN CN2007101592923A patent/CN101469311B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2825646Y (en) * | 2005-09-12 | 2006-10-11 | 吉林农业大学 | Timing automatic catching instrument for fungal spore |
| CN1869201A (en) * | 2005-09-26 | 2006-11-29 | 丽水市林业科学研究所 | Collecting method of ganoderma spore powder and powder collecting device |
Non-Patent Citations (1)
| Title |
|---|
| 黄启东，陈体强.段木灵芝孢子粉的采集.《中国食用菌》.2004,第23卷(第4期),28-29. * |
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| CN101469311A (en) | 2009-07-01 |
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Granted publication date: 20110824 Termination date: 20131228 |