CN218290926U - Adhesion type rapid quantitative disc for number of bacteria in water - Google Patents

Abstract

The utility model discloses a be stained with and glue quick quantitative dish of formula aquatic flora number, it includes: hatching tray, tray cover; the upper surface of the hatching tray is a plane, and a plurality of hatching grooves are distributed on the plane; the edge of the hatching tray is provided with an annular step higher than the upper surface of the hatching tray in a surrounding way; a waste liquid collecting tank is arranged on the annular step, absorbent cotton is arranged in the waste liquid collecting tank, and a waste liquid port communicated to the upper surface of the incubation disc is formed in the waste liquid collecting tank; the bottom of the hatching tray is provided with a supporting seat; the tray cover is used for covering the upper surface of the hatching tray; and a ventilation port is arranged on the side wall of the cover body of the plate cover. The utility model discloses can shorten the experimental period, the flora number in the water sample of comparatively quick and accurate calculation.

Description

Adhesion type rapid quantitative disc for number of bacteria in water

Technical Field

The utility model relates to a water quality testing technical field relates to a be stained with quick quantitative dish of formula aquatic flora number particularly.

Background

With the gradual improvement of the industrialization level of China, the problem of water pollution generally exists in industrial areas of China correspondingly. In recent years, with the rise of environmental awareness, the domestic monitoring of water pollution is gradually strengthened. In the prior art, when detecting the total number of bacteria in water, a plate counting method disclosed in GB 4789.2-2016 total number of bacteria in food safety national standard food microbiology test, is generally used, and the method comprises the following steps: adding (25 g (ml) samples into 225ml of diluent, using a homogenizer to carry out mean value, then carrying out 10-fold dilution, selecting 1-3 sample homogeneous solutions with proper dilution, respectively adding 1ml into sterile culture dishes, adding 15ml-20ml of plate counting plates into each culture dish, uniformly mixing, putting into an incubator at 36 +/-1 ℃ for culturing for 48H +/-2H, then respectively counting the flora number on each plate, and finally calculating the result.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a be stained with and glue quick quantitative dish of formula aquatic flora number, can shorten experimental period, comparatively quick and accurate the flora number in the calculation water sample.

The technical scheme adopted is as follows:

a rapid quantification disc for the number of bacteria in a viscous water comprises: hatching tray and tray cover; the upper surface of the hatching tray is a plane, and a plurality of hatching grooves are distributed on the plane; the edge of the hatching tray is provided with an annular step higher than the upper surface of the hatching tray in a surrounding way; a waste liquid collecting tank is arranged on the annular step, absorbent cotton is arranged in the waste liquid collecting tank, and a waste liquid port communicated with the upper surface of the incubation plate is formed in the waste liquid collecting tank; the bottom of the hatching tray is provided with a supporting seat; the tray cover is used for covering the upper surface of the hatching tray; and a ventilation port is arranged on the side wall of the cover body of the plate cover.

By adopting the technical scheme: firstly, pouring a water sample on the upper surface of the incubation plate, enabling the water sample to enter each incubation groove in the process, and enabling the redundant water sample to enter the waste liquid collecting tank along the waste liquid port and be absorbed by the absorbent cotton. And then, sealing the upper surface of the incubation tray by a tray cover, turning the whole device, and putting the device into a constant-temperature incubator for culture. And (3) hydrolyzing the substrate in the water sample by the enzyme of the microorganism, emitting blue fluorescence under the irradiation of ultraviolet rays, and counting the incubation grooves presenting the blue fluorescence to determine the total number of possible floras in the water sample.

Preferably, in the rapid bacterial population quantifying tray for viscous water: a gap is also formed in the waste liquid collecting tank; the gap is positioned on one side of the waste liquid collecting groove far away from the waste liquid port.

By adopting the technical scheme: after the cotton that absorbs water absorbs full waste liquid, the staff of being convenient for stretches into the finger from the space and will absorb water the cotton and take out from the waste liquid collecting vat, has promoted the cotton efficiency of changing the absorption of water.

Preferably, in the rapid bacterial population quantifying tray for the stained water: the tray cover is made of transparent materials.

By adopting the technical scheme: the staff permeable dish lid is direct to be surveyd hatching recess, has improved experimental efficiency.

Preferably, in the rapid bacterial population quantifying tray for viscous water: the hatching groove is hemispherical.

By adopting the technical scheme: the hemispherical shape can maximize the contact area between the water sample and the inner wall of the hatching tank, and ensure that as much liquid as possible is retained in the hatching tank under the action of surface tension.

Preferably, in the rapid bacterial population quantifying tray for the stained water: the hatching groove is a ball with the radius of 0.15-0.4 cm.

By adopting the technical scheme: the problem of the too big water sample of recess flows out from the recess in avoiding the experimentation, or the recess setting is too little, and the water sample is too little is held back to the single.

Preferably, in the rapid bacterial population quantifying tray for the stained water: the number of the hatching grooves is at least 40, and the hatching grooves are arranged around the center point of the tray body of the hatching tray in a fan shape based on a poise distribution method.

By adopting the technical scheme: the hatching tray structure is more stable, and the hatching grooves which are arranged based on the rime distribution method are convenient to read and count quickly in the experiment process.

Preferably, in the rapid bacterial population quantifying tray for the stained water: the tray cover is provided with a sample inlet, and the sample inlet is positioned in the center of the tray cover.

By adopting the technical scheme: the water sample is poured into the hatching tray by the sample inlet, so that a tray cover does not need to be opened during sample introduction, and the operation process is simplified.

Preferably, in the rapid bacterial population quantifying tray for viscous water: and a handle is arranged on the supporting seat.

By adopting the technical scheme: the hatching tray can be conveniently dumped towards the waste liquid port by utilizing the handle when the waste liquid is discharged, so that the operation of workers is facilitated.

More preferably: the absorbent cotton is cylindrical.

Compared with the prior art, the utility model discloses simple structure easily realizes, can shorten experimental period, comparatively quick and accurate calculation the fungus crowd number in the water sample.

Drawings

Fig. 1 is a schematic structural view of embodiment 1.

Fig. 2 is a schematic structural diagram of fig. 1 after the disk sense is deleted.

The correspondence between each reference numeral and the part name is as follows:

1. hatching trays; 2. hatching a groove; 3. an annular step; 4. a waste liquid collecting tank; 41. a waste liquid port; 5. absorbent cotton; 6. a supporting seat; 7. a handle; 8. a tray cover; 81. a sample inlet; 82. and (4) a ventilation port.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts all belong to the protection scope of the present invention.

Example 1 as shown in fig. 1-2:

a rapid quantification disc for the number of bacteria in a viscous water comprises: an incubation tray 1, a tray cover 8; the upper surface of the hatching tray 1 is a plane, hatching grooves 2 are distributed on the plane, the number of the hatching grooves 2 is at least 40, and the hatching grooves are arranged and distributed around the central point of the tray body of the hatching tray 1 in a fan shape based on a rime distribution method. Each hatching groove 2 is in a hemispherical shape, and the radius of the sphere is 0.15-0.4 cm.

The edge of the hatching tray 1 is provided with an annular step 3 higher than the upper surface of the hatching tray in a surrounding way; a waste liquid collecting groove 4 is formed in the annular step 3, absorbent cotton 5 is installed in the waste liquid collecting groove 4, a waste liquid port 41 communicated to the upper surface of the incubation plate 1 is formed in the waste liquid collecting groove 4, and the waste liquid port 41 is located in the middle section of the waste liquid collecting groove 4; still be equipped with the space in waste liquid collecting vat 4, the space is located the both ends of keeping away from waste liquid mouth 41 on waste liquid collecting vat 4. And a handle 7 is also arranged on the supporting seat 6. The bottom of the hatching tray 1 is provided with a supporting seat 6; the tray cover 8 is used for covering the upper surface of the hatching tray 1; the side wall of the cover body of the tray cover 8 is provided with a ventilation opening 82, and the tray cover 8 is made of transparent material. Be equipped with introduction port 81 on the dish lid 8, introduction port 81 is located dish lid 8 central point and puts.

In practice, the operation is as follows:

step 1: 1ml of water sample is extracted and detected, and dilution can be carried out if the water sample is seriously polluted: 1ml of water sample is absorbed by an aseptic operation method, fully shaken up, then injected into a test tube of 9ml of aseptic normal saline, fully shaken up, and then 1ml is taken for detection:

step 2: placing the incubation tray 1 on a horizontal desktop, pouring the detected water sample obtained in the step 1 into the central point of the tray body of the incubation tray 1 from the sample inlet 81, horizontally shaking the incubation tray 1 tightly attached to the desktop, and distributing the water sample into all grooves of the incubation tray 1. Then, the hatching tray 1 is erected by holding the handle 7, and the excess water sample flows into the waste liquid collecting tank 4 and is absorbed by the absorbent cotton 5.

And step 3: slowly turning over the hatching tray 1, and placing the hatching tray in an incubator at 36 +/-1 ℃ for culturing for 48 +/-2H.

And 4, step 4: the cultured hatching tray 1 was taken out, placed upside down under an ultraviolet lamp, observed at a position of 12cm under an ultraviolet lamp of 6w366nm, the number of wells producing blue fluorescence was recorded, and counted against a maximum possible number of total colonies (mpn) table.

By adopting the above-mentioned operation process: the microorganism carried in the water sample is utilized to decompose one or more enzyme matrixes and then generate signals, so that the counting speed is improved. Specifically, the method comprises the following steps: by providing different enzyme substrates in each hatching recess 2, each designed for a respective different bacterial enzyme, all enzyme substrates produce the same signal when decomposed. During the detection of a water sample, bacteria in the water sample decompose one or more enzyme substrates and then generate a same signal, and when the total number of florae is detected, the signal is fluorescence generated under an ultraviolet lamp with the wavelength of 366 nm. Hydrolyzing substrates in a water sample by using enzymes of microorganisms, culturing for 48 hours at 36 +/-1 ℃, releasing 4-methylumbelliferone to the maximum extent, allowing the 4-methylumbelliferone to emit blue fluorescence under the irradiation of ultraviolet rays with the wavelength of 366nm, counting culture disc wells showing the blue fluorescence, looking up a Most Probable Number (MPN) table, and finally determining the total flora of the original water sample. The method has fewer operation steps, greatly shortens the experimental period, and overcomes the problem of inaccurate flora number caused by human interference factors.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (8)

1. A viscous type rapid quantitative disc for the number of flora in water is characterized by comprising: hatching tray, tray cover; the upper surface of the hatching tray is a plane, and a plurality of hatching grooves are distributed on the plane; the edge of the hatching tray is provided with an annular step higher than the upper surface of the hatching tray in a surrounding way; a waste liquid collecting tank is arranged on the annular step, absorbent cotton is arranged in the waste liquid collecting tank, and a waste liquid port communicated with the upper surface of the incubation plate is formed in the waste liquid collecting tank; the bottom of the hatching tray is provided with a supporting seat; the tray cover is used for covering the upper surface of the hatching tray; and the side wall of the cover body of the plate cover is provided with a ventilation port.

2. The rapid quantification disc for the number of bacteria in the viscous water as claimed in claim 1, wherein: a gap is also formed in the waste liquid collecting tank; the space is positioned on one side, far away from the waste liquid opening, of the waste liquid collecting tank.

3. The rapid quantification disc for the number of bacteria in the viscous water as claimed in claim 1, wherein: the tray cover is made of transparent materials.

4. The rapid quantification disc for the number of bacteria in the viscous water as claimed in claim 1, wherein: the hatching groove is hemispherical.

5. The rapid quantification disc for the number of bacteria in viscous water according to claim 4, wherein: the hatching groove is a ball with the radius of 0.15-0.4 cm.

6. The rapid quantification disc for the number of bacteria in the viscous water as claimed in claim 1, wherein: the number of the hatching grooves is at least 40, and the hatching grooves are arranged around the center point of the tray body of the hatching tray in a fan shape based on a poise distribution method.

7. The rapid quantification disc for the number of bacteria in the viscous water as claimed in claim 1, wherein: the tray cover is provided with a sample inlet, and the sample inlet is positioned in the center of the tray cover.

8. The rapid quantification disc for the number of bacteria in the viscous water as claimed in claim 1, wherein: the supporting seat is provided with a handle.

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