CN107560987B - Atmospheric biological particle concentration calculation method - Google Patents
Atmospheric biological particle concentration calculation method Download PDFInfo
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- CN107560987B CN107560987B CN201710811049.9A CN201710811049A CN107560987B CN 107560987 B CN107560987 B CN 107560987B CN 201710811049 A CN201710811049 A CN 201710811049A CN 107560987 B CN107560987 B CN 107560987B
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Abstract
The invention discloses a method for calculating the concentration of atmospheric biological particles, which comprises the following steps: 1) installing a biological particle sampling device; 2) sampling biological particles in the air for a period of time Δ t; 3) counting the number of biological particles of the slide collected in the sampling device; 4) calculating the concentration of the atmospheric biological particles; in the step 1), the installed biological particle sampling device comprises a rotary driving seat (1), a driving motor (2) is arranged on the rotary driving seat (1), a linkage shaft (3) is arranged on the driving motor (2), and a biological particle collecting part (4) is arranged at the upper end of the linkage shaft (3). The invention provides a novel method for calculating the concentration of the atmospheric biological particles, and realizes accurate calculation of the concentration of the atmospheric biological particles by specifically improving a conventional acquisition device and a counting area, thereby improving the accuracy and reliability of the calculation of the atmospheric biological particles by conventional calculation.
Description
Technical Field
The invention relates to the field of agricultural ecosystem research, in particular to a method for calculating the concentration of atmospheric biological particles.
Background
At present, there are two main methods for calculating the concentration of atmospheric biological particles in the atmosphere by sampling. One is gravity sedimentation, in which a device such as a collection slide is mounted on the ground surface to collect biological particles deposited on landmarks due to gravity. The investigation of biological particles such as pollen by gravity sedimentation has been carried out for decades, and valuable research data is also accumulated. The method is simple and easy to implement, but still has obvious defects. Biological particles such as pollen and the like are small in diameter, small in mass and almost negligible in weight, and float in the air and spread along with wind, so that the gravity settling characteristic is not obvious, and the particles such as pollen and the like collected on the ground cannot reflect the real spreading condition of the particles. Meanwhile, the gravity sedimentation method does not include the concept of air volume, but only the number of particles such as pollen adhered to the glass slide within 24 hours cannot represent the particle concentration in the atmosphere, the correlation between the number of particles adhered to the glass slide and the particle concentration in the atmosphere is poor, and the correlation coefficient is only 0.545. The second is a fluid mechanics method, which utilizes the basic principle of fluid mechanics to implement 24-hour intermittent sampling and measure the concentration of biological particles in the atmosphere. The biggest difference from the gravity settling method is that the fluid mechanics method takes into account the air volume. The method adopts the method that a slide coated with viscous substances such as grease and the like rotates in the air to adhere biological particles such as pollen and the like in the air, and the number of the collected biological particles has a close relation with the moving space volume of the slide and the atmospheric condition. Therefore, how to effectively improve the collection efficiency of the slide and increase the windward probability of the slide is a new development direction for more effectively and accurately collecting the biological particles in the air.
Compared with a domestic TH-001-60 type pollen sampler, the RotorodSampler sampler based on the fluid mechanics method is simpler and easy to operate. Rotood Sampler, the slide is mounted in a plastic holder and the collection efficiency of the windward side of the slide varies with distance from the axis of rotation. The farther away from the axis of rotation the slide area is, the higher the collection efficiency. This is because the smaller the circumference of the slide area rotating closer to the rotation axis, the less the volume of air fanned, and the less air contacted, and therefore the less the chance of air particles being contacted, and the lower the natural collection efficiency. Therefore, many collection experiments using rotoodsampler select a region of the slide that is far from the axis of rotation, select a substantially linear region, count under the microscope, and compute to estimate the total particulate matter. In selecting linear regions, the experimenter routinely selects linear regions 0.6625mm wide and 25mm long for microscopic bioparticle counting according to the characteristics of the rotood Sampler apparatus.
However, the collection efficiency will vary in view of the variation of the frontal area of the slide, and most experiments will use the calculated particle concentration multiplied by a set collection efficiency value, and some studies will use 0.64 to obtain the final particle concentration, based on the experience of previous studies.
Therefore, how to provide a new biological particle counting selection and calculation method to more accurately and effectively improve the collection efficiency of the slide and increase the windward probability of the slide is a problem faced by the prior art.
Therefore, those skilled in the art are devoted to developing a new method for selecting and calculating the air biological particle count, so as to improve the accuracy of the air total particle concentration calculated by the sampler.
Disclosure of Invention
In view of the above-mentioned drawbacks of the prior art, the technical problem to be solved by the present invention is to provide a new method for calculating the concentration of airborne biological particles, including selecting a counting area, so as to improve the accuracy of calculating the concentration of airborne particles in the atmosphere.
In order to achieve the above object, the present invention provides an atmospheric bio-particle concentration calculation method, comprising the following steps:
1) installing a biological particle sampling device;
2) sampling biological particles in the air for a period of time Δ t;
3) counting the number of biological particles of the slide collected in the sampling device;
4) calculating the concentration of the atmospheric biological particles;
in the step 1), the installed biological particle sampling device comprises a rotary driving seat, the rotary driving seat is provided with a driving motor, a linkage shaft is arranged on the driving motor, and a biological particle collecting part is arranged at the upper end of the linkage shaft; the biological particle collecting part comprises a first slide support and a second slide support which are identical in structure, the first slide support and the second slide support are mutually and vertically crossed, the crossed parts are respectively overlapped with the middle points of the first slide support and the second slide support, and the crossed parts are fixed on the linkage shaft; a first microscope slide and a second microscope slide are symmetrically arranged at two ends of the first slide bracket at intervals along the midpoint; and a third microscope slide and a fourth microscope slide are symmetrically arranged at two ends of the second slide bracket along the midpoint at intervals.
It is noted that, in step 3), a linear region having a width of 0.81 to 0.85mm and a length of 25mm was selected as a counting region under a microscope at positions 18 to 21mm from the outer edge of the slide on the first to fourth slides, respectively.
In the prior art, an integrated slide is adopted for sampling, and a region with the width of 0.6625mm and the length of 25mm is generally adopted for sampling at a position 10mm away from the outer edge of the slide, however, experiments show that the selection of the sampling region generally causes sampling pollution due to the installation and the detachment of the slide, and the sampling width of 0.6625mm is easy to count, but the structure of the integrated collection slide is combined with the structure of the integrated collection slide, the concentration of biological particles in the atmosphere cannot be accurately reflected, the technical scheme combines the improvement of the structure of a sampling device, and tests prove for multiple times, the sampling position is adjusted to be 18-21mm away from the outer edge of the slide, the width of the sampling region is selected to be 0.81-0.85mm, and the calculated concentration of the biological particles in the atmosphere can be closer to the true value, so that the sampling width is determined to be 0.81-0.85 mm.
Preferably, in step 4), the concentration of the biological particles in the atmosphere is calculated according to the following formula:
wherein Ni is the number of biological particles in the calculation region on the first to fourth slides;
s is the sampling area of each sampling slide;
RS is the rotation speed of the universal driving shaft, and the unit is r/s;
Δ t is the sampling time.
Preferably, in step 1), the heights of the first to fourth microscope slides are all set to be 25mm, and the lengths are set to be 40 mm; the lengths of the first slide support and the second slide support are both set to be 92.5 mm; and uniform silicone grease is arranged on the first microscope slide, the second microscope slide and the fourth microscope slide.
Preferably, in step 1), the biological particle sampling device is installed in a vertical direction, and the installation height H is set to be 0.35-8m (the sedimentation velocity of biological particles in the air is adjusted according to the height of the leaf crown of the plant to be researched).
The novel method for calculating the concentration of the atmospheric biological particles, which comprises the selection of the counting area, is provided, so that the accuracy and the stability of the concentration of the atmospheric biological particles calculated by the method are higher.
The invention has the beneficial effects that: the invention provides a novel method for calculating the concentration of the atmospheric biological particles, and realizes accurate calculation of the concentration of the atmospheric biological particles by specifically improving a conventional acquisition device and a counting area, thereby improving the accuracy and reliability of the calculation of the atmospheric biological particles by conventional calculation.
Drawings
Fig. 1 is a schematic structural diagram of a biological particle sampling apparatus.
Fig. 2 is an assembled structural view of the sampling device of fig. 1.
Detailed Description
The invention is further illustrated by the following examples in conjunction with the accompanying drawings:
as shown in fig. 1 to 2, a method for calculating the concentration of atmospheric bio-particles includes the following steps:
1) installing a biological particle sampling device;
2) sampling biological particles in the air for a period of time Δ t;
3) counting the number of biological particles of the slide collected in the sampling device;
4) calculating the concentration of the atmospheric biological particles;
in the step 1), the installed biological particle sampling device comprises a rotary driving seat 1, the rotary driving seat 1 is provided with a driving motor 2, a linkage shaft 3 is arranged on the driving motor 2, and the upper end of the linkage shaft 3 is provided with a biological particle collecting part 4; the biological particle collecting part 4 comprises a first slide support 5 and a second slide support 6 which have the same structure, the first slide support 5 and the second slide support 6 are mutually and vertically crossed, the crossed points are respectively overlapped with the middle points of the first slide support 5 and the second slide support 6, and the crossed points are fixed on the universal driving shaft 3; a first microscope slide 7 and a second microscope slide 8 are symmetrically arranged at two ends of the first slide bracket 5 along the midpoint at intervals; the third microscope slide 9 and the fourth microscope slide 10 are symmetrically arranged at two ends of the second slide bracket 6 at intervals along the midpoint; in step 3, a linear region S having a width of 0.83mm and a length of 25mm was selected as a counting region under a microscope at a position 18mm from the outer edge of the slide on each of the first to fourth slides.
In other embodiments, other values in the range of 18-21mm from the outer edge of the slide, such as 19mm, 20mm, 21mm, may be selected to achieve the same technical effect.
In other embodiments, other values in the range of 0.81-0.85mm, such as 0.81mm, 0.82mm, 0.84mm, 0.85mm, can be selected to achieve the same technical effect.
In step 4), the concentration of the biological particles in the atmosphere is calculated according to the following formula:
wherein Ni is the number of biological particles in the calculation region on the first to fourth slides;
s is the sampling area of each sampling slide;
RS is the rotation speed of the universal driving shaft, and the unit is r/s;
Δ t is the sampling time.
Taking pollen sampling in the air as an example, setting the sampling time delta t to be 2h as 7200 s;
s＝0.83*25*10-6
through observation, the Ni values on the slide glass 7, 8, 9 and 10 are respectively 14, 16, 8 and 13;
the rotation speed of the RS is set to be 27.2 revolutions per second;
the concentration C of the biological particles in the atmosphere is calculated to be 3.138grains/m2/s。
In the step 1), the heights h of the first to fourth microscope slides are set to be 25mm, and the lengths l are set to be 40 mm; the length L of the first slide support 5 and the second slide support 6 are both set to 92.5 mm; uniform silicone grease is arranged on the first microscope slide, the second microscope slide and the fourth microscope slide.
In the step 1), the biological particle sampling device is installed along the vertical direction, and the installation height H is set to be 0.35-8m (the sedimentation speed of biological particles in the air is adjusted according to the height of a leaf crown of a research plant).
By the method, the accurate calculation of the concentration of the biological particles in the atmosphere can be better realized, and the stability of the calculation structure can be further improved.
The foregoing detailed description of the preferred embodiments of the invention has been presented. It should be understood that numerous modifications and variations could be devised by those skilled in the art in light of the present teachings without departing from the inventive concepts. Therefore, the technical solutions available to those skilled in the art through logic analysis, reasoning and limited experiments based on the prior art according to the concept of the present invention should be within the scope of protection defined by the claims.
Claims (3)
1. A method for calculating the concentration of atmospheric biological particles is characterized by comprising the following steps: the method comprises the following steps:
1) installing a biological particle sampling device;
2) sampling biological particles in the air for a period of time Δ t;
3) counting the number of biological particles of the slide collected in the sampling device;
4) calculating the concentration of the atmospheric biological particles;
in the step 1), the installed biological particle sampling device comprises a rotary driving seat (1), the rotary driving seat (1) is provided with a driving motor (2), a linkage shaft (3) is arranged on the driving motor (2), and the upper end of the linkage shaft (3) is provided with a biological particle collecting part (4);
the biological particle collecting part (4) comprises a first slide support (5) and a second slide support (6) which are identical in structure, the first slide support (5) and the second slide support (6) are mutually perpendicular and crossed, the crossed points are respectively superposed with the middle points of the first slide support (5) and the second slide support (6), and the crossed points are fixed on the linkage shaft (3);
a first microscope slide (7) and a second microscope slide (8) are symmetrically arranged at two ends of the first slide bracket (5) at intervals along the midpoint;
a third microscope slide (9) and a fourth microscope slide (10) are symmetrically arranged at two ends of the second slide support (6) at intervals along the midpoint;
in the step 3), linear areas (S) with the width of 0.81-0.85mm and the length of 25mm are selected as counting areas under a microscope at positions 18-21mm away from the outer edge of the slide on the first to fourth slides respectively;
in step 4), the concentration of the biological particles in the atmosphere is calculated according to the following formula:
wherein Ni is the number of biological particles in the calculation region on the first to fourth slides;
s is the sampling area of each sampling slide;
RS is the rotation speed of the universal driving shaft, and the unit is r/s;
Δ t is the sampling time.
2. The method for calculating the concentration of atmospheric bio-particles according to claim 1, wherein:
in the step 1), the heights (h) of the first to fourth microscope slides are all set to be 25mm, and the length (1) is set to be 40 mm;
the lengths (L) of the first slide support (5) and the second slide support (6) are set to be 92.5 mm;
and uniform silicone grease is arranged on the first microscope slide, the second microscope slide and the fourth microscope slide.
3. The method for calculating the concentration of atmospheric bio-particles according to claim 1, wherein:
in the step 1), the biological particle sampling device is installed along the vertical direction, and the installation height (H) is set to be 0.35-8 m.
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| CN101469311A (en) * | 2007-12-28 | 2009-07-01 | 中国科学院沈阳应用生态研究所 | Large-sized fungal sporule field timing collecting device |
| CN101806676A (en) * | 2010-03-23 | 2010-08-18 | 中国科学院研究生院 | Pollen collector |
| CN103900852A (en) * | 2014-04-09 | 2014-07-02 | 中国科学院东北地理与农业生态研究所 | Atmospheric pollen collector |
| CN105388093A (en) * | 2015-11-02 | 2016-03-09 | 北京大学 | Online monitoring system of pollen in atmosphere |
| CN105850382A (en) * | 2016-04-15 | 2016-08-17 | 中国农业科学院草原研究所 | Pollen sampling collecting tool |
| CN205491817U (en) * | 2016-03-15 | 2016-08-24 | 东北林业大学 | Forest pollen receiver |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US6514721B2 (en) * | 2001-07-03 | 2003-02-04 | Biochem Technologies, Inc. | Air sampler for pathogens and psychrometrics |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101469311A (en) * | 2007-12-28 | 2009-07-01 | 中国科学院沈阳应用生态研究所 | Large-sized fungal sporule field timing collecting device |
| CN101806676A (en) * | 2010-03-23 | 2010-08-18 | 中国科学院研究生院 | Pollen collector |
| CN103900852A (en) * | 2014-04-09 | 2014-07-02 | 中国科学院东北地理与农业生态研究所 | Atmospheric pollen collector |
| CN105388093A (en) * | 2015-11-02 | 2016-03-09 | 北京大学 | Online monitoring system of pollen in atmosphere |
| CN205491817U (en) * | 2016-03-15 | 2016-08-24 | 东北林业大学 | Forest pollen receiver |
| CN105850382A (en) * | 2016-04-15 | 2016-08-17 | 中国农业科学院草原研究所 | Pollen sampling collecting tool |
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