CN107560987A - A kind of air bioparticle concentration computational methods - Google Patents
A kind of air bioparticle concentration computational methods Download PDFInfo
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- CN107560987A CN107560987A CN201710811049.9A CN201710811049A CN107560987A CN 107560987 A CN107560987 A CN 107560987A CN 201710811049 A CN201710811049 A CN 201710811049A CN 107560987 A CN107560987 A CN 107560987A
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Abstract
The invention discloses a kind of air bioparticle concentration computational methods, comprise the following steps：1) biologic grain sampling apparatus is installed；2) biologic grain in air is sampled in a period of time Δ t；3) the biologic grain number of slide is gathered in statistic sampling device；4) air bioparticle concentration is calculated；In step 1), the biologic grain sampling apparatus of installation includes rotation driving seat (1), the rotation driving seat (1) is provided with motor (2), universal driving shaft (3) is provided with the motor (2), universal driving shaft (3) upper end is provided with biologic grain collection portion (4).The present invention is by providing a kind of new air bioparticle concentration computational methods, pass through the specific improvement to conventional Christmas device and counting region, the accurate calculating to air bioparticle concentration is realized, improves the accuracy and reliability of the calculating of conventionally calculation air biologic grain.
Description
Technical field
The present invention relates to agroecosystem research field, more particularly to a kind of air bioparticle concentration calculating side
Method.
Background technology
At present, mainly there are two kinds of basic skills by sampling calculating air bioparticle concentration in air.First, gravity sinks
Drop method, the devices such as collection slide are arranged on earth's surface, collect the biologic grain that terrestrial reference is deposited to because of gravity.With gravitational settling
Many decades have been carried out in the investigation that method carries out the biologic grains such as pollen, are also accumulated from the research data of preciousness.This method is simple
It is easy, but still have clearly disadvantageous.The biologic grain diameter such as pollen is small, and quality is small, and weight almost can be ignored,
Float in air, propagate with the wind, therefore the particle such as gravitational settling feature unobvious, the pollen of ground acquisition can not reflect that its is true
Propagation condition.Meanwhile settling methods does not include the concept of volume of air, glutinous pollen is simply glued in 24 hours on sheet glass
Deng numbers of particles, the granule density in air, the granule number glued on slide and the granule density correlation in air can not be characterized
Difference, coefficient correlation are only 0.545.Second, Hydrostatic injection, this method utilizes hydromechanical general principle, implements 24 hours
Discontinuous sampling, determine the bioparticle concentration in air.Maximum difference, is that Hydrostatic injection considers with settling methods
Volume of air.This method adheres to the pollen in air using the slide for having smeared the emplastics such as grease is rotated in atmosphere
Deng biologic grain, the biologic grain number collected and the spatial volume of slide movement, close relationship is suffered from atmospheric conditions.
Therefore, the collecting efficiency of slide how is effectively improved, increases slide probability windward, is more effective, more accurately implements air
The new development direction of the collection of middle biologic grain.
Sampler based on hydrodynamic methods, relative to domestic TH-001-60 type pollen samplers, Rotorod
Sampler samplers are more simple, easily operated.Rotorod Sampler, slide are arranged in plastic stent, and slide is met
The collecting efficiency in wind face can change with the distance apart from rotary shaft.The slide region more remote apart from rotary shaft, it collects effect
Rate is higher.Because the girth of the slide area rotation nearer apart from rotary shaft is smaller, the volume of air agitated is fewer, contact
Air it is fewer, so the air particles chance touched is fewer, natural collecting efficiency can be low.Therefore, much using Rotorod
Sampler collection experiment, all can the remote slide region of selected distance rotary shaft, select a basic range of linearity, carry out
Counting under microscope, then realize by computing the estimation of total particulate matter.In the selection of the range of linearity, experimenter according to
The characteristics of Rotorod Sampler devices, conventional selection width are 0.6625mm, and the range of linearity that length is 25mm carries out micro-
Biologic grain statistics under mirror.
But can be different in view of the change in slide region windward, collecting efficiency, most of experiment can be according to above studying
Experience, a collecting efficiency value of setting is multiplied by with the granule density of calculating, part research uses 0.64, obtains final
Grain concentration.
Therefore, how a kind of new biologic grain is provided and counts selection and computational methods, allows to accurately and effectively carry
The collecting efficiency of high slide, increase slide probability windward, be prior art problems faced.
Therefore those skilled in the art are directed to developing a kind of new air biologic grain counting selection and computational methods, carry
High sampler calculates the accuracy of the air total particle concentration of gained.
The content of the invention
In view of the drawbacks described above of prior art, the technical problems to be solved by the invention are to provide including selecting count block
A kind of new air bioparticle concentration computational methods including domain, improve the accuracy for calculating atmospheric air granule density.
To achieve the above object, the invention provides a kind of air bioparticle concentration computational methods, comprise the following steps：
1) biologic grain sampling apparatus is installed；
2) biologic grain in air is sampled in a period of time Δ t；
3) the biologic grain number of slide is gathered in statistic sampling device；
4) air bioparticle concentration is calculated；
In step 1), the biologic grain sampling apparatus of installation includes rotation driving seat, and the rotation driving seat is provided with drive
Motor is moved, is provided with universal driving shaft on the motor, the universal driving shaft upper end is provided with biologic grain collection portion；The biology
Particle collection portion includes the slide support of structure identical first and the second slide support, first slide support and the second slide
Support is mutually perpendicular to intersect, and cross part distinguishes the first slide support and the midpoint of the second slide support overlaps, and cross part is fixed
On universal driving shaft；The first slide support both ends are symmetrically provided with the first microslide and the second micro- glass along midpoint
Piece；The second slide support both ends are symmetrically provided with the 3rd microslide and the 4th microslide along midpoint.
It is worth noting that, in step 3), respectively apart from slide outer edge 18-21mm's on first to fourth slide
Position, a width of 0.81-0.85mm is selected, the long 25mm range of linearity is as the counting region under microscope.
In the prior art, due to being sampled using the slide of integration, it is typically employed in the position away from slide outer 10mm
It is 0.6625mm to put collection width, and length is that 25mm region is sampled, and still, the choosing of the sample area is found through experiments that
Selecting would generally be because the installation and unloading of slide causes sampling to be polluted, while also and although 0.6625mm sampling width is easy to
Count, but combine the integral collecting slide structure can not exactly in reactive atmosphere biologic grain concentration, this skill
Art scheme combines the improvement to sampling apparatus structure, is verified by test of many times, sample position is adjusted to apart from slide outer
18-21mm, and a width of 0.81-0.85mm is selected on sampling width, and then the air bioparticle concentration being calculated more can
Close to actual value, it is thus determined that selection sampling width is 0.81-0.85mm.
Preferably, in step 4), the concentration of biologic grain in air is calculated according to the following formula：
Wherein, Ni is the biologic grain number of the zoning on the first to the 4th slide；
S is the Sampling Area of every piece of sampling slide；
RS be universal driving shaft velocity of rotation, unit r/s；
Δ t is sample time.
Preferably, in step 1), first to fourth microslide is highly disposed as 25mm, and length is arranged to
40mm；The length of first slide support and the second slide support is disposed as 92.5mm；First to fourth microscope
Uniform silicone grease is provided with slide.
Preferably, in step 1), the biologic grain sampling apparatus is vertically installed, and setting height(from bottom) H is set
0.35-8m (according to the integral shroud height of research plant, sinking speed is adjusted biologic grain in atmosphere).
Provide a kind of new air bioparticle concentration computational methods including selection counting region so that utilize
The air bioparticle concentration degree of accuracy that this method calculates gained is higher, and stability is more preferable.
The beneficial effects of the invention are as follows：The present invention is led to by providing a kind of new air bioparticle concentration computational methods
The specific improvement to conventional Christmas device and counting region is crossed, the accurate calculating to air bioparticle concentration is realized, improves
The accuracy and reliability that conventionally calculation air biologic grain calculates.
Brief description of the drawings
Fig. 1 is the structural representation of biologic grain sampling apparatus.
Fig. 2 is the assembly structure figure of sampling apparatus in Fig. 1.
Embodiment
The invention will be further described with reference to the accompanying drawings and examples：
As shown in Figure 1 to Figure 2, a kind of air bioparticle concentration computational methods, comprise the following steps：
1) biologic grain sampling apparatus is installed；
2) biologic grain in air is sampled in a period of time Δ t；
3) the biologic grain number of slide is gathered in statistic sampling device；
4) air bioparticle concentration is calculated；
In step 1), the biologic grain sampling apparatus of installation includes rotation driving seat 1, and rotation driving seat 1 is provided with driving
Motor 2, universal driving shaft 3 is provided with motor 2, the upper end of universal driving shaft 3 is provided with biologic grain collection portion 4；Biologic grain gathers
Portion 4 includes structure identical the first slide support 5 and the second slide support 6, the first slide support 5 and the phase of the second slide support 6
Mutual square crossing, the first slide support 5 is distinguished in crosspoint and the midpoint of the second slide support 6 overlaps, and crosspoint is fixed on linkage
On axle 3；The both ends of first slide support 5 are symmetrically provided with the first microslide 7 and the second microscope slide 8 along midpoint；Second
The both ends of slide support 6 are symmetrically provided with the 3rd microslide 9 and the 4th microslide 10 along midpoint；In step 3,
A width of 0.83mm, long 25mm range of linearity S are selected on one to the 4th slide apart from slide outer edge 18mm position respectively
For as the counting region under microscope.
In other embodiment, other values that can also be in the 18-21mm scopes of chosen distance slide outer, as 19mm,
20mm, 21mm, to reach identical technique effect.
In other embodiment, counting region width can also be selected as the other values in 0.81-0.85mm scopes, such as
0.81mm, 0.82mm, 0.84mm, 0.85mm, to reach identical technique effect.
In step 4), the concentration of biologic grain in air is calculated according to the following formula：
Wherein, Ni is the biologic grain number of the zoning on the first to the 4th slide；
S is the Sampling Area of every piece of sampling slide；
RS be universal driving shaft velocity of rotation, unit r/s；
Δ t is sample time.
Exemplified by sampling pollen in atmosphere, sample time Δ t is set as 2h=7200s；
S=0.83*25*10-6
By observation, the Ni numerical value on slide 7,8,9,10 is respectively 14,16,8,13；
RS velocity of rotation is set as 27.2 revolutions per seconds；
It is 3.138grains/m that bioparticle concentration C in air, which is calculated,2/s。
In step 1), first to fourth microslide height h is disposed as 25mm, and length l is arranged to 40mm；First glass
The length L of the slide support 6 of plate rack 5 and second is disposed as 92.5mm；It is provided with first to fourth microslide uniformly
Silicone grease.
In step 1), biologic grain sampling apparatus is vertically installed, setting height(from bottom) H be arranged on 0.35-8m (according to
The integral shroud height of plant according to the study, sinking speed is adjusted biologic grain in atmosphere).
By this method, the accurate calculating to bioparticle concentration in air can be more preferably realized, and one can be entered
Step improves the stability for calculating structure.
Preferred embodiment of the invention described in detail above.It should be appreciated that one of ordinary skill in the art without
Creative work can is needed to make many modifications and variations according to the design of the present invention.Therefore, all technologies in the art
Personnel are available by logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Technical scheme, all should be in the protection domain being defined in the patent claims.
Claims (4)
1. a kind of air bioparticle concentration computational methods, it is characterized in that：Comprise the following steps：
1) biologic grain sampling apparatus is installed；
2) biologic grain in air is sampled in a period of time Δ t；
3) the biologic grain number of slide is gathered in statistic sampling device；
4) air bioparticle concentration is calculated；
In step 1), the biologic grain sampling apparatus of installation includes rotation driving seat (1), and the rotation driving seat (1) is provided with
Motor (2), universal driving shaft (3) is provided with the motor (2), universal driving shaft (3) upper end is provided with biologic grain
Collection portion (4)；
The biologic grain collection portion (4) includes the slide support of structure identical first (5) and the second slide support (6), described
First slide support (5) is mutually perpendicular to intersect with the second slide support (6), and the first slide support (5) and second are distinguished in crosspoint
The midpoint of slide support (6) is overlapped, and crosspoint is fixed on universal driving shaft (3)；
First slide support (5) both ends are symmetrically provided with the first microslide (7) and the second microscope slide along midpoint
(8)；
Second slide support (6) both ends are symmetrically provided with the 3rd microslide (9) and the 4th microscope glass along midpoint
Piece (10)；
In step 3), selected a width of apart from slide outer edge 18-21mm position respectively on first to fourth slide
0.81-0.85mm, long the 25mm range of linearity (S) are as the counting region under microscope.
2. air bioparticle concentration computational methods as claimed in claim 1, it is characterized in that：
In step 4), the concentration of biologic grain in air is calculated according to the following formula：
<mrow>
<mi>C</mi>
<mo>=</mo>
<mrow>
<mo>(</mo>
<munderover>
<mo>&Sigma;</mo>
<mrow>
<mi>i</mi>
<mo>=</mo>
<mn>1</mn>
</mrow>
<mn>4</mn>
</munderover>
<mfrac>
<msub>
<mi>N</mi>
<mi>i</mi>
</msub>
<mrow>
<mi>&Delta;</mi>
<mi>t</mi>
<mo>&times;</mo>
<mi>s</mi>
<mo>&times;</mo>
<mi>R</mi>
<mi>S</mi>
</mrow>
</mfrac>
<mo>)</mo>
</mrow>
<mo>/</mo>
<mn>4</mn>
</mrow>
Wherein, Ni is the biologic grain number of the zoning on the first to the 4th slide；
S is the Sampling Area of every piece of sampling slide；
RS be universal driving shaft velocity of rotation, unit r/s；
Δ t is sample time.
3. air bioparticle concentration computational methods as claimed in claim 1, it is characterized in that：
In step 1), the first to fourth microslide height (h) is disposed as 25mm, and length (l) is arranged to 40mm；
First slide support (5) and the length (L) of the second slide support (6) are disposed as 92.5mm；
Uniform silicone grease is provided with first to fourth microslide.
4. air bioparticle concentration computational methods as claimed in claim 1, it is characterized in that：
In step 1), the biologic grain sampling apparatus is vertically installed, and setting height(from bottom) (H) is arranged on 0.35-8m.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108844870A (en) * | 2018-08-08 | 2018-11-20 | 重庆交通大学 | PM based on optical fiber structure10And PM2.5Detection instrument device and system |
| CN110057729A (en) * | 2019-03-12 | 2019-07-26 | 天津大学 | A kind of human body particulate matter passively settles concentration determination method |
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| US20030008341A1 (en) * | 2001-07-03 | 2003-01-09 | Spurrell Leon Bryan | Adjustable air sampler for pathogens and psychrometrics |
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| CN103900852A (en) * | 2014-04-09 | 2014-07-02 | 中国科学院东北地理与农业生态研究所 | Atmospheric pollen collector |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108844870A (en) * | 2018-08-08 | 2018-11-20 | 重庆交通大学 | PM based on optical fiber structure10And PM2.5Detection instrument device and system |
| CN108844870B (en) * | 2018-08-08 | 2021-09-21 | 重庆交通大学 | PM based on optical fiber structure10And PM2.5Probe instrument apparatus and system |
| CN110057729A (en) * | 2019-03-12 | 2019-07-26 | 天津大学 | A kind of human body particulate matter passively settles concentration determination method |
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| CN107560987B (en) | 2020-03-20 |
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