CN201817489U - Device used for high flux medicine sensitive test - Google Patents
Device used for high flux medicine sensitive test Download PDFInfo
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- CN201817489U CN201817489U CN2009203506791U CN200920350679U CN201817489U CN 201817489 U CN201817489 U CN 201817489U CN 2009203506791 U CN2009203506791 U CN 2009203506791U CN 200920350679 U CN200920350679 U CN 200920350679U CN 201817489 U CN201817489 U CN 201817489U
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- independent cell
- medicine carrying
- sensitive test
- bacterium
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Abstract
The utility model discloses a device used for high flux medicine sensitive test, comprising a cultivating box, a medicine-carrying device and an accessory bacteria-sucking device, wherein the cultivating box is internally provided with small independent chambers which are not communicated with one other so as to guarantee that the medicine sensitive test results of the small chambers can not influence each other; with the medicine-carrying device, the preparation method is simple, the operation is easy, batch production is realized, and the production efficiency is high. By adopting the device, 6-50 medicine sensitive tests can be carried at one time, thus realizing high flux medicine sensitive test; the flexibility of load bearing material of medicine-sensitive paper is good, the medicine sensitive paper can be fully contacted with culture medium, thus guaranteeing the reliability of medicine sensitive test. The device can be mainly used for testing medicine sensitivity of microbial strain or floras such as disease germ, human/animal indigenous flora and the like, solves the problems in the prior art and product that high-flux tests can not be carried out, the manufacture process of the medicine-carrying device consumes much time, the medicine-carrying device can not be completely buckled with the culture medium, and the like.
Description
Technical field
The utility model relates to a kind of device that is used for antibiotics susceptibility test, particularly a kind of device that is used for the high-throughput antibiotics susceptibility test.
Background technology
Drug sensitive test is a kind of important means of auxiliary detection pathogenic micro-organism, and antibiotics susceptibility test method commonly used mainly comprises two kinds of minimum inhibitory concentration method (MIC method) and disk diffusion methods (K-B method).The antibiotics susceptibility test method that wherein the K-B method is clinical and the laboratory is the most frequently used.
The device of the existing K-B of utilization method testing drug susceptibility comprises two big classes: its common trait is that the culture apparatus that contains substratum is all arranged, and difference is to have or not the medicine carrying device that carries drug sensitive test paper.
Have only its shortcoming of antibiotics susceptibility test device of culture apparatus no-load medicine device to be: need the user to provide drug sensitive test paper for oneself, during use drug sensitive test paper is affixed on the media surface that is coated with bacterium liquid, operational condition is had relatively high expectations; Each culture apparatus only can be put into 4-6 piece of drug sensitive test paper, can't realize the high-throughput test.
The antibiotics susceptibility test device that has culture apparatus and medicine carrying device simultaneously, as utility model patent 200320131549.1, its shortcoming is: 1) marker space of placing single drug sensitive test paper is arranged in the culture apparatus, but an end of each marker space is interconnected, though such design is easy to the can solid medium, but cause sometimes that the antibacterial zone of single drug sensitive test paper diffuses into a slice in each marker space, can't judge the inhibition zone size of the drug sensitive test paper of spreading area correspondence, thereby influence the interpretation of whole drug sensitivity tests; 2) this type of the device with the medicine carrying device, plastic ring or the bars made by hard plastic of adopting carry drug sensitive test paper more, during making, the producer need utilize tackiness agent that the drug sensitive test paper for preparing is pasted on plastic ring or the bar singly, consuming time, production efficiency very low (make a drug allergy ring or bar and need 15-20 minute), and the medicine carrying device causes drug sensitive test paper to come off from carrier because tackiness agent viscosity is bad easily, makes troubles to the user; 3) owing to the drug allergy ring or the bar of carrying drug sensitive test paper are made by hard plastic, hardness is bigger, area is less, easy deformation, may fasten not tight when causing embedding the cultivation box, the substratum that has been coated with bacterium liquid in part drug sensitive test paper on drug allergy ring or the bar and the culturing room can't fully contact, and causes this part drug sensitive test to come to nothing or the result is inaccurate and then cause the whole susceptibility experimental result can't interpretation.
Summary of the invention
At the problems such as " can't the high-throughput tests, consuming time, the medicine carrying device of medicine carrying device production process fasten incomplete with substratum " that above-mentioned prior art and product exist, the utility model provides a kind of device that is used for the high-throughput antibiotics susceptibility test.This device comprises that cultivating box, medicine carrying device and accessory inhales the bacterium device.Its advantage is: cultivating in the box has the independent cell that is not connected, and has guaranteed that the antibiotics susceptibility test result in each independent cell does not interfere with each other; With medicine carrying device preparation method simple, easily operation can be produced the production efficiency height in batches; This device once can carry out more than 6 simultaneously, and 50 antibiotics susceptibility tests of as many as have been realized the high-throughput antibiotics susceptibility test; The carrier snappiness of drug sensitive test paper is good, and drug sensitive test paper can fully contact with substratum, guarantees the reliability of antibiotics susceptibility test.
The device that is used for the high-throughput antibiotics susceptibility test that the utility model provides comprises: cultivate box, medicine carrying device and optional accessory and inhale the bacterium device.Wherein cultivate box and be made up of the independent cell more than 2, the number of independent cell is generally more than 6, preferred 10-50.Each independent cell is rectangle or square, and the shape size is consistent, is arranged, wide about 0.5-3 centimetre, is preferably 1-2 centimetre.Be filled with solid medium in each independent cell, the height of solid medium is lower than cultivates the about 0.5-5 millimeter of cell sidewall height, is preferably low 1-3 millimeter.Cultivation tape lid can have sealing-ring in the lid, prevents that the substratum dehydration in each independent cell is dry and cracked.Cultivation is closed scale, is used to measure the inhibition zone size of drug sensitive test paper, is convenient to interpretation antibiotics susceptibility test result.
Medicine carrying device described in the utility model, its surface bears has drug sensitive test paper.The size shape of device with cultivate box and match, place it in the media surface that can make contained drug sensitive test paper contact each independent cell respectively on the cultivation box, avoid antibiotics susceptibility test because of drug sensitive test paper contacts with substratum insufficient test result instability that causes.The preferred version of medicine carrying device is to utilize the medicine carrying membrane carrying to contain the drug sensitive test paper of different pharmaceutical.Medicine carrying membrane is characterized in that snappiness is good, and one side can be covered glue, is pasted with shape, the identical drug sensitive test paper of size on the medicine carrying membrane, leaves the space between adjacent drug sensitive test paper, guarantee contained drug sensitive test paper can be respectively with each independent cell in solid medium fully contact.
Accessory is inhaled the bacterium device and is comprised suction bacterium layer and plane support thereof, and plane support mostly is back up pad, and certain thickness and hardness are arranged, and has one to be fit to hand-held handle on it, is convenient to operation.Inhale that the bacterium layer requires good water absorption, certain thickness is arranged, high resilience, as non-woven fabrics, ventilative cotton, film paper etc., it can invest on the flat support plate by modes such as stickup, fastenings.The size and the shape of inhaling bacterium layer and upholder thereof match with the cultivation box, guarantee that the bacterium liquid of being drawn can evenly, intactly be applied to the media surface in the independent cell.
Medicine carrying device described in the utility model, the method for its batch process is: 1) drug solution with suitable concentration soaks filter paper, the suitable way drying, the susceptibility bar of cutting, be cut into strip is standby, and the width of susceptibility bar is less than the cell width; 2) the susceptibility bar that will contain different pharmaceutical successively vertically sticks on the medicine carrying membrane, leaves the space between adjacent susceptibility bar; 3) select suitable width crosscut medicine carrying membrane, make and contain drug sensitive test paper identical shaped and big or small, that contain different pharmaceutical on the medicine carrying membrane.
Medicine carrying device described in the utility model, its using method is: the one side that medicine carrying membrane is contained the susceptibility scraps of paper covers a top of arranging independent cell, and applying light is attached on the media surface of each cell each drug sensitive test paper respectively.
Its purposes of the device that is used for the high-throughput antibiotics susceptibility test that the utility model provides is: the 1) drug susceptibility of test pathogenic bacterium or flora; 2) drug susceptibility of test human or animal autochthonous flora is to estimate human or animal's resistance risk.
The usage of device provided by the utility model comprises following mode: 1) test is with a kind of bacterium or the same flora susceptibility to different pharmaceutical, and each cell is tested a kind of medicine; 2) different bacterium of test and/or flora are to the susceptibility with a kind of medicine, and each cell is tested a kind of bacterium or a flora; Or, 3) combination of above-mentioned two kinds of methods, etc.
Description of drawings
Accompanying drawing the 1, the 2nd, the synoptic diagram of cultivation box
Accompanying drawing 3 is the synoptic diagram that vertically are pasted with the medicine carrying membrane of the susceptibility bar that contains different pharmaceutical
Accompanying drawing the 4, the 5th, the medicine carrying schematic representation of apparatus
Accompanying drawing 6 is synoptic diagram of inhaling the bacterium device
Accompanying drawing 7 is the synoptic diagram that are placed with the cultivation box of medicine carrying device
Accompanying drawing 8 is the synoptic diagram that are placed with the medicine carrying device, irritate the cultivation box that substratum is arranged
More than 1 be the independent cell of cultivating in the box among each figure, be placed with substratum in it, the 7th, the locular wall of independent cell, the 8th, the susceptibility bar, the 2nd, medicine carrying membrane, the 3rd, drug sensitive test paper, the 4th, plane support, the 5th, inhale the bacterium layer, the 9th, handle, the 6th, solid medium.
Embodiment
With reference to Figure of description, the embodiment of this utility model is described.
The utility model comprises that mainly cultivating box, medicine carrying device and accessory inhales the bacterium device.The cultivation box is rectangular, and scale is arranged on it, is convenient to read the inhibition zone size.Cultivating in the box has 30 cells 1 alone, and the shape size of each independent cell is consistent, and long 4 centimetres, wide 1.5 centimetres, be arranged.Solid medium 6 is housed in the cell.During concrete the making, utilize quantitative sample injector (commercially available or customized) with solid medium 6 cans in independent cell 1, the surface of solid medium 6 is a little less than the locular wall 7 of independent cell, low 2 millimeters, as illustrate shown in Figure 8.
The making processes of medicine carrying device: 1) drug solution with suitable concentration soaks filter paper, the suitable way drying, and the susceptibility bar 8 of cutting, be cut into strip is standby, and the width of susceptibility bar 8 is less than the width of independent cell 1; 2) the susceptibility bar 8 that will contain different pharmaceutical successively vertically sticks on the medicine carrying membrane 2, leaves the space between adjacent susceptibility bar, as shown in Figure 3; 3) select 1 centimetre of crosscut medicine carrying membrane of width, make and contain drug sensitive test paper identical shaped and big or small, that contain different pharmaceutical on the medicine carrying membrane, as shown in Figure 4.
The medicine carrying membrane that contains drug sensitive test paper identical shaped and big or small, that contain different pharmaceutical is the medicine carrying device of present embodiment.Its using method is: the one side that the medicine carrying device is contained the susceptibility scraps of paper 3 covers a top of arranging independent cell 1, and applying light is attached on the media surface of each independent cell 1 each drug sensitive test paper 3 respectively.
Inhale the bacterium device in the present embodiment and comprise handle 9, plane support 4, suction bacterium layer 5.Handle 9 is positioned at the middle part of plane support 4, and plane support 4 is thin plates that are made of plastics.Inhale bacterium layer 5 and made by the non-woven fabrics of 4 mm thick, this non-woven fabrics good water absorption, certain thickness and elasticity are arranged, inhaling bacterium layer 5 can be fixed on the plane support 4 with the good double faced adhesive tape of viscosity.After evenly coating bacterium liquid on the solid medium 6 in the independent cell 1, the medicine carrying device is put in cultivates in the box, shown in Fig. 7,8.Just can judge the antibiotics susceptibility test result after cultivating on demand.
Claims (10)
1. a device that is used for antibiotics susceptibility test is characterized in that comprising and cultivates box and medicine carrying device, wherein cultivates box and is made up of the independent cell more than 2, in each cell substratum is arranged; The medicine carrying apparatus surface has drug sensitive test paper.
2. device according to claim 1 is characterized in that cultivating box and is made up of the independent cell more than 6.
3. device according to claim 1 is characterized in that cultivating box and is made up of 10-50 independent cell.
4. device according to claim 1 is characterized in that:
1) each independent cell is rectangle or square, and the shape size is consistent, is arranged;
2) locular wall of independent cell is a little more than media surface.
5. according to claim 1,2,3 or 4 described devices, it is characterized in that the wide 0.5-3 of independent cell centimetre.
6. according to claim 1,2,3 or 4 described devices, it is characterized in that independent cell wide is 1-2 centimetre.
7. according to claim 1,2,3 or 4 described devices, it is characterized in that the locular wall of independent cell is higher than media surface 0.5-5 millimeter.
8. according to claim 1,2,3 or 4 described devices, it is characterized in that the locular wall of independent cell is higher than media surface 1-3 millimeter.
9. according to claim 1,2,3 or 4 described devices, it is characterized in that:
1) carrier of medicine carrying device is the good medicine carrying membrane of a kind of snappiness, is pasting the shape drug sensitive test paper identical with size on it, leaves the space between the adjacent scraps of paper;
2) size shape of medicine carrying device matches with the cultivation box.
10. according to claim 1,2,3 or 4 described devices, it is characterized in that: be furnished with the bacterium of suction device, inhale the bacterium device by good water absorption, form by certain thickness, whippy suction bacterium layer and plane support, inhale the bacterium laminating on plane support, upholder has suitable hand-held handle, and the size and the shape of inhaling bacterium layer and upholder thereof match with the cultivation box.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009203506791U CN201817489U (en) | 2009-12-31 | 2009-12-31 | Device used for high flux medicine sensitive test |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009203506791U CN201817489U (en) | 2009-12-31 | 2009-12-31 | Device used for high flux medicine sensitive test |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201817489U true CN201817489U (en) | 2011-05-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009203506791U Expired - Lifetime CN201817489U (en) | 2009-12-31 | 2009-12-31 | Device used for high flux medicine sensitive test |
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| Country | Link |
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| CN (1) | CN201817489U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109112064A (en) * | 2018-08-31 | 2019-01-01 | 泰州市春达动物药业饲料有限公司 | A kind of susceptibility kit and preparation method thereof applied to aquaculture |
-
2009
- 2009-12-31 CN CN2009203506791U patent/CN201817489U/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109112064A (en) * | 2018-08-31 | 2019-01-01 | 泰州市春达动物药业饲料有限公司 | A kind of susceptibility kit and preparation method thereof applied to aquaculture |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20110504 |
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| CX01 | Expiry of patent term |