CN209923311U

CN209923311U - Automatic fermentation cylinder that transplants of probiotic seed

Info

Publication number: CN209923311U
Application number: CN201920153172.0U
Authority: CN
Inventors: 宋锦安; 韩迪; 郑建丰; 刘伟
Original assignee: Runying Bioengineering (shanghai) Co Ltd
Current assignee: Biogrowing Co ltd
Priority date: 2019-01-29
Filing date: 2019-01-29
Publication date: 2020-01-10
Anticipated expiration: 2029-01-29

Abstract

The utility model belongs to the technical field of the fermentation equipment technique and specifically relates to an automatic fermentation cylinder of transfering of benefit fungus seed, including the gas cylinder, the switch board, the dona tub, large-scale fermentation cylinder, the level detection sensor, online cell density appearance, the tank bottoms valve, the blowoff valve, the valve of transfering a kind, the automatic valve, adopt the pipe connection between the gas outlet of gas cylinder and the income gas port of dona tub, be equipped with level detection sensor and online cell density appearance in the dona tub, the signal output part of level detection sensor is connected with the liquid level signal input part of switch board, the signal output part of online cell density appearance is connected with the cell density signal input part of switch board. Compared with the prior art, the utility model, designed the automatic fermentation cylinder of transfering of benefit fungus seed, through the fermentation condition in level detection sensor, the online cell density appearance detects the seed jar to according to the detection condition through transfering kind valve and pipeline switching to large-scale fermentation cylinder, with realize accurate, pollution-free switching, thereby guarantee the product quality and reduce fermentation time.

Description

Automatic fermentation cylinder that transplants of probiotic seed

Technical Field

The utility model belongs to the technical field of fermentation equipment technique and specifically relates to an automatic fermentation cylinder that transplants of benefit fungus seed.

Background

The production process of the probiotic products generally comprises the steps of activating a strain freeze-drying tube and then gradually enlarging and culturing until the required production quantity is reached, wherein the strain freeze-drying tube and a large-scale fermentation tank are used as the most important part in the whole fermentation link, and the method has important influence on the determination of the whole fermentation end point and the quality of the final product.

If the time for transferring the seeding tank into the large-scale fermentation tank is earlier than the optimum time, the fermentation time of the large-scale fermentation tank can be prolonged due to the low number of viable bacteria in the seeding tank, and the enterprise cost is increased. If the time for transferring the seed tank to the large fermentation tank is later than optimal, the quality of the final product is affected because of the reduced viability of the probiotics in the seed tank.

At present, probiotic fermentation enterprises generally determine the fermentation end point of a seed tank by manually measuring the absorbance value of fermentation liquor, and then manually transfer the fermentation end point to a large-scale fermentation tank. This approach is prone to contamination and risk of inaccurate metering.

Therefore, it is necessary to design an automatic probiotic seed transfer fermentation tank to realize accurate and pollution-free transfer, thereby ensuring product quality and reducing fermentation time.

Disclosure of Invention

The utility model aims at overcoming the not enough of prior art, providing an automatic fermentation cylinder that transplants of probiotic seed to realize accurate, pollution-free switching, thereby guarantee the product quality and reduce fermentation time.

In order to achieve the purpose, the utility model relates to an automatic fermentation cylinder of transfering of benefit fungus seed, including gas cylinder, switch board, dona tub, large-scale fermentation cylinder, liquid level detection sensor, online cell density appearance, tank bottoms valve, blowoff valve, transfering a kind valve, automatic valve, its characterized in that: the gas outlet of the gas cylinder is connected with the gas inlet of the seed tank by a pipeline, a liquid level detection sensor and an online cell density instrument are arranged in the seed tank, the signal output end of the liquid level detection sensor is connected with the liquid level signal input end of the control cabinet, the signal output end of the online cell density instrument is connected with the cell density signal input end of the control cabinet, the bottom of the seed tank is connected with one end of the tank bottom valve, the other end of the tank bottom valve is divided into two paths to be respectively connected with one end of the blow-down valve and one end of the seed conversion valve, the other end of the seed conversion valve is connected with one end of the automatic valve I by a seed conversion pipeline, the other end of the automatic valve I is divided into two paths to be respectively connected with one end of the automatic valve II and one end of the automatic valve III, the other end of the automatic valve III is connected with the input port of the large, The actuator of the automatic valve II is connected with the actuator of the automatic valve III.

And the information transmission end of the control cabinet is connected with a computer.

And a gas source valve, a pressure reducing device, a pressure gauge and a spherical valve are arranged on a pipeline between the gas cylinder and the seed tank.

The front surface of the seeding tank is provided with a window, and the surface of the window is provided with scales.

Compared with the prior art, the utility model, designed the automatic fermentation cylinder of transfering of benefit fungus seed, through the fermentation condition in level detection sensor, the online cell density appearance detects the seed jar to according to the detection condition through transfering kind valve and pipeline switching to large-scale fermentation cylinder, with realize accurate, pollution-free switching, thereby guarantee the product quality and reduce fermentation time.

Drawings

Fig. 1 is a structural diagram of the present invention.

Detailed Description

The invention will now be further described with reference to the accompanying drawings.

Referring to fig. 1, the solid lines are pipes, and the dotted lines are circuits and signal lines. The utility model relates to an automatic fermentation cylinder of transfering of benefit fungus seed of living, including gas cylinder, switch board, dona tub, large-scale fermentation cylinder, liquid level detection sensor, online cell density appearance, tank bottoms valve, blowoff valve, transfer kind valve, automatic valve. The gas outlet of the gas bottle 1 is connected with the gas inlet of the seed tank 15 by a pipeline, a liquid level detection sensor 6 and an online cell density instrument 8 are arranged in the seed tank 15, the signal output end of the liquid level detection sensor 6 is connected with the liquid level signal input end of a control cabinet 17, the signal output end of the online cell density instrument 8 is connected with the cell density signal input end of the control cabinet 17, the bottom of the seed tank 15 is connected with one end of a tank bottom valve 18, the other end of the tank bottom valve 18 is divided into two paths and is respectively connected with one end of a blowoff valve 10 and a seed transferring valve 9, the other end of the seed transferring valve 9 is connected with one end of an automatic valve 11 by a seed transferring pipeline, the other end of the automatic valve 11 is divided into two paths and is respectively connected with one end of an automatic valve II 12 and one end of an automatic valve III 13, the other end of the automatic valve III 13 is connected with the input, The actuator of the seed changing valve 9, the actuator of the automatic valve I11, the actuator of the automatic valve II 12 and the actuator of the automatic valve III 13 are connected, and the information transmission end of the control cabinet 17 is connected with the computer 16. The utility model discloses carry out parameter setting by computer 16, if admission pressure control, fermentation terminal point cell concentration, change kind zymotic fluid volume, automatically-controlled valve switch etc. switch 17 links computer 16 and leads the function condition of adjusting each control spare part, realizes automatic fermentation and changes kind the function.

The utility model discloses in, be equipped with air supply valve 2, pressure relief device 3, manometer 4 and ball valve 5 on the pipeline between gas cylinder 1 and the dona tub 15, mainly be for transport suitable gas in toward the dona tub 15, guarantee the condition of malleation in the messenger dona tub 15 under cultivate, this pressure also is a kind of power of transferring simultaneously.

In order to ensure the good operation of the equipment system, a window 7 is arranged on the front surface of the seed tank 15, scales are arranged on the surface of the window 7, and the window 7 is favorable for checking the amount of the transferred seeds. Meanwhile, for the sake of safety, the corresponding automatic valve can select a valve which is integrated manually and automatically so as to be operated. Thus, the advantages and convenience that both manual operation and automatic operation can be performed can be realized.

The utility model discloses the during operation will be prepared liquid medium and pour into clean dona tub 15, carry out high temperature sterilization to it. During the high-temperature sterilization treatment of the liquid culture medium, the pipeline and the valve at the top of the fermentation tank can be used for carrying out steam sterilization on the seed transferring pipeline. The steam can be discharged from the automatic valve three 13 of the large-scale fermentation tank 14 through the automatic valve two 12, and can be discharged from the other part through the automatic valve one 11, the seed changing valve 9, the blowdown valve 10 and corresponding pipelines. And after the end, closing the blowdown valve 10 and the automatic valve two 12.

After the liquid culture medium is sterilized, the temperature of the liquid culture medium is reduced to a proper temperature, and then inoculation culture is carried out. Parameters such as culture conditions, end point cell density, air inlet pressure, and transfer inoculum size are set by the computer 16. And introducing gas from the top of the seed tank 15 through the gas cylinder 1 to reach the set gas pressure, and maintaining the pressure.

At this time, the rotary pipeline can be sterilized again independently, for example, by using bypass steam, a part of the steam is discharged through the blow-down valve 10, a part of the steam is discharged through the seed changing valve 9, the automatic valve I11 and the automatic valve II 12, after the process is finished, the blow-down valve 10 and the automatic valve II 12 are closed, positive pressure is kept, and the seeds in the seed tank 15 are waiting for maturity.

According to the setting of the computer 16, the culture of the seeding tank 15 is continuously carried out, the cell concentration of the fermentation liquor is monitored in real time by the linear cell density instrument 8, and the fermentation can be stopped when the cell concentration detected by the linear cell density instrument 8 reaches a certain set value: the on-line cell densitometer 8 transmits signals to the processor and computer 16 of the control cabinet 17. After being processed, the output signals are transmitted to actuators of four automatic valves, namely a tank bottom valve 18, a seed transferring valve 9, an automatic valve I11 and an automatic valve III 13. The four automatic valves are automatically opened, and the seed culture solution enters the large-scale fermentation tank 14 through the tank bottom valve 18, the seed transferring valve 9, the automatic valve I11, the automatic valve III 13 and the corresponding seed transferring pipeline. At this time, the liquid level detecting sensor 6 measures the liquid level change, and the magnitude of the transferred amount is determined by the correlation calculation program. When the seed transferring amount reaches the set parameters, the processor of the control cabinet 17 transmits output signals to the actuators of the tank bottom valve 18, the seed transferring valve 9, the automatic valve I11 and the automatic valve III 13, the tank bottom valve 18 is closed, and the seed transferring valve 9, the automatic valve I11 and the automatic valve III 13 are closed successively. At this point, the transfer work is finished.

When the equipment is cleaned, the blow-down valve 10 is opened, and the residual fermentation residual liquid is discharged into a sewage treatment system to carry out corresponding treatment work.

The utility model discloses a fermentation condition in liquid level detection sensor 6, the online cell density appearance 8 detection dona tub 15 to according to the detection condition through change kind of valve 9 and pipeline switching to large-scale fermentation cylinder 14 at optimum time, with realize accurate, pollution-free switching. The optimum time for transferring seeds ensures the number of live bacteria in the seeding tank 15, reduces the fermentation time of the large-scale fermentation tank 14 and lowers the enterprise cost. Meanwhile, the activity of the probiotics in the tank is also kept, and the product quality is ensured.

Claims

1. The utility model provides an automatic fermentation cylinder of transfering of probiotic seed, includes gas cylinder, switch board, dona tub, large-scale fermentation cylinder, liquid level detection sensor, online cell density appearance, tank bottoms valve, blowoff valve, changes kind valve, automatic valve, its characterized in that: the gas outlet of the gas cylinder (1) is connected with the gas inlet of the seed tank (15) by a pipeline, a liquid level detection sensor (6) and an online cell density meter (8) are arranged in the seed tank (15), the signal output end of the liquid level detection sensor (6) is connected with the liquid level signal input end of a control cabinet (17), the signal output end of the online cell density meter (8) is connected with the cell density signal input end of the control cabinet (17), the bottom of the seed tank (15) is connected with one end of a tank bottom valve (18), the other end of the tank bottom valve (18) is divided into two paths to be respectively connected with a blow-down valve (10) and one end of a seed conversion valve (9), the other end of the seed conversion valve (9) is connected with one end of an automatic valve I (11) by a seed conversion pipeline, the other end of the automatic valve I (11) is divided into two paths to be respectively connected with one ends of an automatic valve II (12, the other end of the automatic valve III (13) is connected with an input port of the large-scale fermentation tank (14), and five signal output ends of the control cabinet (17) are respectively connected with an actuator of the tank bottom valve (18), an actuator of the seed transferring valve (9), an actuator of the automatic valve I (11), an actuator of the automatic valve II (12) and an actuator of the automatic valve III (13).

2. The automatic probiotic seed transfer fermentation tank of claim 1, characterized in that: the information transmission end of the control cabinet (17) is connected with the computer (16).

3. The automatic probiotic seed transfer fermentation tank of claim 1, characterized in that: and an air source valve (2), a pressure reducing device (3), a pressure gauge (4) and a ball valve (5) are arranged on a pipeline between the air bottle (1) and the seed tank (15).

4. The automatic probiotic seed transfer fermentation tank of claim 1, characterized in that: the front surface of the seeding tank (15) is provided with a window (7), and the surface of the window (7) is provided with scales.