CN101824380A - Production method of fungus test bottle - Google Patents
Production method of fungus test bottle Download PDFInfo
- Publication number
- CN101824380A CN101824380A CN 200910079171 CN200910079171A CN101824380A CN 101824380 A CN101824380 A CN 101824380A CN 200910079171 CN200910079171 CN 200910079171 CN 200910079171 A CN200910079171 A CN 200910079171A CN 101824380 A CN101824380 A CN 101824380A
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- bottle
- test bottle
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- fungus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
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- Microbiology (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to a production method of a fungus test bottle, belonging to the technical field of microorganism detection methods. The invention is characterized in that 0.5 percent of peptone, 0.1 percent of monopotassium phosphate, 0.05 percent of magnesium sulfate, 1.0 percent of glucose and 98.35 percent of distilled water are respectively taken at normal temperature, the chemicals are sequentially dissolved by using the distilled water, are added into a container and are mixed and fully agitated, and 0.5mol/L sulfuric acid solution is used for regulating a pH value to 3.8-4.0 to prepare required culture solution; and the prepared culture solution is filled in bottles by 9.0ml per bottle, and then the bottles are capped, sealed, sterilized at high temperature and cooled to obtain the fungus test bottles. The invention has the advantages that the production process is simple, the reliability is high and the economy and the practicability are high; and by adopting the test bottle, the number of fungi in sample water to be detected can be accurately determined, the operation and the use are simple and the work efficiency is improved.
Description
Technical field:
The present invention relates to a kind of production method of fungus test bottle, belong to using microbe detection method technical field.
Background technology:
In the real work of research and production, usually need microbial numbers is measured.Can monitor and estimate its pollution level to the microbioassay in the environment such as water body, air, soil; Can estimate to the microbioassay in tap water and the industrial circulating cooling water whether it up to standard; Mensuration to microbe population in the oilfield sewage water filling then helps to estimate sewage injection water quality etc.Therefore, the mensuration of microbe population has crucial directive function and realistic meaning to the research and production real work.
Microbioassay method commonly used has the method for plate culture count and disappearance dilution method.The method of plate culture count is the principle design that can grow a bacterium colony according to each bacterium that lives, get the bacteria suspension of a constant volume, make a series of doubling dilution, then quantitative diluent being carried out flat board cultivates, according to colony number of turning out and extension rate, calculate the viable count in the culture; And the disappearance dilution method is water sample to be determined to be injected into step by step inoculate dilution in the test tube, to the last in test tube till the asepsis growth, then according to bacterial growth situation and extension rate, calculates the number of bacterium in the water sample.These two kinds of methods all have tangible deficiency, and the method for plate culture count measurement result error is bigger, and the operation of disappearance dilution method is very loaded down with trivial details, time-consuming.
Ubiquity fungi in industrial circulating cooling water.The existence of fungi can be polluted cooling water quality, forms biological mud and then influences heat exchange, and participate in corrosion.For effectively control fungi harm, need fungi is accurately measured.At present, generally adopt the fungi in the method for plate culture count mensuration industrial circulating cooling water both at home and abroad, but this method measurement result error is bigger, and loaded down with trivial details, time-consuming.Therefore, there are open defect in existing traditional test technology and means of testing, far can not satisfy requirements of actual production.
Summary of the invention:
In order to overcome the deficiencies in the prior art, the object of the present invention is to provide a kind of production method of fungus test bottle, make the test bottle of being produced can measure fungi bacterium number in the water sample rapidly and accurately.
The present invention realizes above-mentioned purpose by following technical solution.
At normal temperatures, get peptone 0.5%, potassium primary phosphate 0.1%, sal epsom 0.05%, glucose 1.0% respectively, distilled water 98.35%, with distilled water it is dissolved successively then, and join in the container and to mix, fully to shake up, with the sulphuric acid soln of 0.5mol/L the pH value of solution is adjusted to 3.8--4.0, promptly is mixed with nutrient solution; With the molded cillin bottle of 12ml clean up, drying, with liquid dispenser institute's prepared culture is packed in the bottle by every bottle of 9.0ml nutrient solution, add a cover then, seal; To use the high-temperature steam Autoclave at 121 ℃, 1.05Kg/cm through adding a cover the test bottle that seals
2Carry out high-temperature sterilization under the condition 18 minutes, and promptly made fungus test bottle after the cooling.
The present invention has following beneficial effect compared with prior art:
1, the production method of a kind of fungus test bottle of the present invention, production process is simple, and good reliability is economical and practical.
2, the fungus test bottle that the present invention produced can be measured the bacterium number of the fungi in the water sample rapidly and accurately, manipulate simple, the result is directly accurate.
The pH of the nutrient solution that 3, the present invention produced is 3.8--4.0, only suitable fungal growth, and the microorganism of being turned out is a fungi, and test job can not be subjected to the interference of bacterium.
Embodiment:
(with preparation 1L nutrient solution is example at normal temperatures, by that analogy), take by weighing peptone 5.0g, potassium primary phosphate 1.0g, sal epsom 0.5g, glucose 10.0g respectively, with distilled water it is dissolved successively then, and join in the 1L volumetric flask mix, again with the distilled water constant volume to the 1L scale marks, fully shake up, the pH value of solution is adjusted to 3.8--4.0, promptly be mixed with the required nutrient solution of fungus test bottle with the sulphuric acid soln of 0.5mol/L.With the molded cillin bottle of 12ml clean up, drying, with liquid dispenser institute's prepared culture is packed in the bottle by every bottle of 9.0ml nutrient solution branch, add a cover with A-13 type medical rubber plug then, seal through the special-purpose sealing device with common antibiotic glass bottle aluminium lid, use the high-temperature steam Autoclave at last at 121 ℃, 1.05Kg/cm
2Under the condition test bottle is concentrated sterilization 18 minutes, promptly make fungus test bottle after the cooling.
When the bacterium of adopting fungus test bottle to measure the fungi in the water sample to be measured is counted, system adopts disappearance dilution method principle, water sample to be measured is injected the test bottle dilution step by step with asepsis injector to be cultivated, to the last a test bottle does not have till the fungal growth, calculates the number of fungi in the water sample to be measured according to the multiple of dilution.Can adopt following method and step:
(1) the 1ml injector for medical purpose of preparation number Zhi Xiangtong cleans up back high-temperature sterilization (121 ℃ of high-pressure steam sterilizing pans, 1.05Kg/cm with distilled water
2Under sterilized 18 minutes), cool off standby.
(2) according to fungi in the water sample to be measured roughly what, the fungus test bottle that several are above-mentioned is lined up one group, and compile to go up sequence number 1,2,3,4 successively ...
(3) get 1.0mL water sample to be measured with the asepsis injector after the above-mentioned sterilization and be injected in No. 1 fungus test bottle, fully shook 10 seconds.
(4) get in addition and take out 1.0mL liquid in No. 1 fungus test bottle of an asepsis injector after shake up and be injected in No. 2 fungus test bottles, fully shook 10 seconds.
(5) get in addition again and take out 1.0mL liquid in No. 2 fungus test bottles of an asepsis injector after shake up and be injected in No. 3 fungus test bottles, fully shook 10 seconds.
(6) repeat the aforesaid operations program successively, until till last bottle.
(7) one group of fungus test bottle behind the above-mentioned adding water sample to be measured is placed in 30 ℃ of (or in situ temperature) incubators cultivates,, judge the fungi growth situation through the variation in the observation test bottle after 3 days.
(8) judging criterion: the nutrient solution no change in the fungus test bottle that no fungi exists, the interior nutrient solution of fungus test bottle that has fungi to exist then becomes muddy feculence, and the nutrient solution surface has the fungi mycoderm to occur.
(9) according to the situation of indication fungal growth in the fungus test bottle and the bottle number of the test bottle that fungal growth is arranged, the 1 fungi bacterium number that can calculate in the water sample to be measured of tabling look-up.
Single bottle of fungi maximum possible of table 1 value
| Fungal growth test bottle number is arranged | A bacterium number/mL -1 |
| ??1??2??3??4??5 | ??1-10??10-100??100-1000??1000-10000??10000-100000 |
Claims (4)
1. the production method of a fungus test bottle is characterized in that adding the nutrient solution of being made up of peptone, potassium primary phosphate, sal epsom, glucose and distilled water in bottle, seals through adding a cover, high-temperature steam sterilizes and make.
2. the production method of a kind of fungus test bottle according to claim 1, it is characterized in that getting respectively at normal temperatures peptone 0.5%, potassium primary phosphate 0.1%, sal epsom 0.05%, glucose 1.0%, distilled water 98.35%, with distilled water it is dissolved successively then, and join in the container and to mix, fully to shake up, with the sulphuric acid soln of 0.5mol/L the pH value of solution is adjusted to 3.8--4.0, promptly is mixed with nutrient solution.
3. the production method of a kind of fungus test bottle according to claim 2, it is characterized in that with the molded cillin bottle of 12ml clean up, drying, with liquid dispenser institute's prepared culture is packed in the bottle by every bottle of 9.0ml nutrient solution, add a cover then, seal.
4. the production method of a kind of fungus test bottle according to claim 3 is characterized in that and will use the high-temperature steam Autoclave at 121 ℃, 1.05Kg/cm through adding a cover the test bottle that seals
2Carried out high-temperature sterilization under the condition 18 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910079171 CN101824380A (en) | 2009-03-04 | 2009-03-04 | Production method of fungus test bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910079171 CN101824380A (en) | 2009-03-04 | 2009-03-04 | Production method of fungus test bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101824380A true CN101824380A (en) | 2010-09-08 |
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ID=42688544
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200910079171 Pending CN101824380A (en) | 2009-03-04 | 2009-03-04 | Production method of fungus test bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101824380A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102653718A (en) * | 2011-03-03 | 2012-09-05 | 长江大学 | Production method of sulfur bacteria test bottle |
| CN103305587A (en) * | 2012-03-09 | 2013-09-18 | 长江大学 | Production method for microzyme test bottle |
-
2009
- 2009-03-04 CN CN 200910079171 patent/CN101824380A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102653718A (en) * | 2011-03-03 | 2012-09-05 | 长江大学 | Production method of sulfur bacteria test bottle |
| CN103305587A (en) * | 2012-03-09 | 2013-09-18 | 长江大学 | Production method for microzyme test bottle |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20100908 |