CN209872937U - Liquid fermentation culture bacillus device - Google Patents
Liquid fermentation culture bacillus device Download PDFInfo
- Publication number
- CN209872937U CN209872937U CN201920226110.8U CN201920226110U CN209872937U CN 209872937 U CN209872937 U CN 209872937U CN 201920226110 U CN201920226110 U CN 201920226110U CN 209872937 U CN209872937 U CN 209872937U
- Authority
- CN
- China
- Prior art keywords
- fermentation
- air
- cooling water
- bacillus
- sterile
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 58
- 230000004151 fermentation Effects 0.000 title claims abstract description 58
- 241000193830 Bacillus <bacterium> Species 0.000 title claims abstract description 22
- 239000007788 liquid Substances 0.000 title claims abstract description 17
- 239000000498 cooling water Substances 0.000 claims abstract description 24
- 238000011081 inoculation Methods 0.000 claims abstract description 5
- 238000009826 distribution Methods 0.000 claims abstract 2
- 238000012258 culturing Methods 0.000 claims description 8
- 238000005070 sampling Methods 0.000 claims description 6
- 239000011148 porous material Substances 0.000 claims 1
- 239000001963 growth medium Substances 0.000 abstract description 7
- 230000008676 import Effects 0.000 abstract description 2
- 238000013019 agitation Methods 0.000 abstract 1
- 239000012530 fluid Substances 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 241000193749 Bacillus coagulans Species 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 241000881860 Paenibacillus mucilaginosus Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 229940054340 bacillus coagulans Drugs 0.000 description 1
- 239000007633 bacillus mucilaginosus Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000010907 mechanical stirring Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000003910 polypeptide antibiotic agent Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model belongs to the technical field of fermentation engineering, concretely relates to bacillus device is cultivateed in liquid fermentation, including the fermentation cylinder, manhole, gas vent and inoculation mouth, aseptic compressed air import, aseptic air conveying pipe, cooling water jacket, drain hole etc.. The utility model discloses be provided with the cooling water jacket and be convenient for control fermentation culture temperature, the air distributor who uses a plurality of vertical distributions adopts the air agitation culture medium, and the guarantee culture medium is fully ventilated and the misce bene, does not have the noise, practices thrift consumption of power, and reasonable in design is fit for popularizing and applying.
Description
Technical Field
The utility model belongs to the technical field of fermentation engineering, concretely relates to liquid fermentation cultivates bacillus device.
Background
Bacillus belongs to a family of bacteria, and spores (endospores) can be formed in the bacteria. Common bacillus includes bacillus subtilis, bacillus licheniformis, bacillus mucilaginosus, bacillus coagulans and the like. The bacillus has fast growth speed and strong metabolic capability, can generate enzymes such as protease, amylase, cellulase, lysozyme and the like, can generate various organic acids, and can also generate antibacterial substances such as antibacterial peptide and the like, so the bacillus is widely applied to the biological pesticide, biological organic fertilizer and feed industry. The fermentation culture of the bacillus can adopt liquid fermentation or solid fermentation. Because liquid state fermentation has the advantages of high fermentation speed, small occupied area, difficult contamination, high automation control level and the like, most enterprises in industrial production adopt the liquid fermentation technology to culture the bacillus at present.
The fermentation tank used when bacillus is cultured by liquid fermentation has two types: mechanically agitated fermentors and airlift fermentors, most of which use mechanically agitated fermentors. The mechanical stirring type fermentation tank has the defects of high noise, high mechanical power consumption, easy bacterial contamination and the like. The traditional airlift fermentation tank has the problems of easy blockage of the air distributor, insufficient oxygen supply when the viscosity of materials is high and uneven mixing of culture mediums because sterile air is introduced from the air distributor arranged at the bottom.
SUMMERY OF THE UTILITY MODEL
The utility model solves the problems and provides a liquid fermentation bacillus culturing device without noise, small power consumption and uniform culture medium mixing.
The utility model discloses a following technical scheme realizes:
the utility model relates to a liquid fermentation bacillus culturing device, which comprises a fermentation tank, wherein the fermentation tank is a cylinder, and the top of the fermentation tank is provided with a manhole, an exhaust port and an inoculation port; the center of the top of the fermentation tank is provided with a sterile compressed air inlet which is connected with a sterile air conveying pipe, and the sterile air conveying pipe is vertically connected with a plurality of air distributors; a feed inlet and a sampling port are formed in the middle upper part of the fermentation tank, a cooling water jacket is arranged in the middle of the fermentation tank, and a cooling water inlet and a cooling water outlet are formed in the cooling water jacket; the fermentation cylinder bottom is provided with the drain hole, the fermentation cylinder below is provided with the support.
Preferably, the number of the air distributors is 5-10, the air distributors and the sterile air conveying pipe are arranged in the vertical direction, and the air distributors are arranged in the direction of the sterile air conveying pipe at equal intervals.
Preferably, the air distributor is evenly distributed with small holes in each direction, and the diameter of the small holes is 2-5 mm.
Preferably, the included angle between the manhole and the sterile air delivery pipe is 30-50 degrees.
Compared with the prior art, the utility model apparent advantage that has does:
(1) without a stirring device, the power consumption is greatly reduced.
(2) The medium was agitated with air without noise.
(3) The shaft seal device is not arranged, so that bacteria are not easily infected.
(4) The air distributor is arranged in the middle of fermentation, and is not easy to block.
Drawings
FIG. 1 is a schematic structural diagram of a device for culturing Bacillus by liquid fermentation.
The parts in the figures are numbered:
1. a fermentation tank; 2. a manhole; 3. a sterile compressed air inlet; 4. an exhaust port; 5. inoculating; 6. a sterile air delivery tube; 7. a feed inlet; 8. a sampling port; 9. a cooling water jacket; 10. a cooling water outlet; 11. a cooling water inlet; 12. an air distributor; 13. a discharging port; 14. and (4) a bracket.
Detailed Description
The following describes in detail embodiments of the present invention with reference to the drawings.
See fig. 1.
The utility model relates to a liquid fermentation bacillus culturing device, which comprises a fermentation tank 1, wherein the fermentation tank 1 is a cylinder, and the top of the fermentation tank 1 is provided with a manhole 2, an exhaust port 4 and an inoculation port 5; the center of the top of the fermentation tank 1 is provided with a sterile compressed air inlet 3, the sterile compressed air inlet 3 is connected with a sterile air conveying pipe 6, and the sterile air conveying pipe 6 is vertically connected with a plurality of air distributors 12; a feed inlet 7 and a sampling port 8 are arranged at the middle upper part of the fermentation tank 1, a cooling water jacket 9 is arranged at the middle part of the fermentation tank, and a cooling water inlet 11 and a cooling water outlet 10 are arranged on the cooling water jacket 9; the bottom of fermentation cylinder 1 is provided with drain hole 13, fermentation cylinder 1 below is provided with support 14.
Preferably, the number of the air distributors 12 is 5-10, the air distributors 12 are arranged in a direction perpendicular to the sterile air conveying pipe 6, and the air distributors 12 are arranged at equal intervals in the direction of the sterile air conveying pipe 6.
Preferably, the air distributor 12 has small holes with diameters of 2-5mm distributed evenly in each direction.
Preferably, the angle between the manhole 2 and the sterile air delivery pipe 6 is 30-50 degrees.
The working process is as follows:
the culture medium after the sterilization is carried into fermentation cylinder 1 from feed inlet 7 in, from inoculation mouth 5 access bacillus seed liquid, aseptic compressed air gets into aseptic air conveyer pipe 6 through aseptic compressed air import 3, aseptic air passes through a plurality of air distributor 12, in the injection culture medium of all directions from, make in the culture medium on the one hand with air intensive mixing, guarantee the required a large amount of oxygen of bacillus growth, on the other hand makes the culture medium be in the homogeneous mixing state of boiling form, the air that escapes from the zymotic fluid discharges outside the jar through gas vent 4. Can follow sampling port 8 sampling monitoring fermentation process in the fermentation process, the heat production can be produced in the bacillus growth process, when the zymotic fluid temperature surpassed the settlement temperature when 30 ℃, open cooling water inlet 11, the cooling water outlet 10 of cooling water jacket 9, make cooling water jacket 9 inner loop cooling water, reduce the zymotic fluid temperature, when the zymotic fluid temperature dropped to the settlement temperature, close cooling water inlet 11 and cooling water outlet 10, when the fermentation was finished, open drain hole 13 and carry out the blowing, collect the thalli. When necessary, after the fermentation is finished, an operator can enter the fermentation tank from the manhole 2 to perform cleaning, maintenance and other work.
Claims (4)
1. The device for culturing the bacillus by liquid fermentation is characterized by comprising a fermentation tank (1), wherein the fermentation tank (1) is a cylinder, and the top of the fermentation tank (1) is provided with a manhole (2), an exhaust port (4) and an inoculation port (5); the center of the top of the fermentation tank (1) is provided with a sterile compressed air inlet (3), the sterile compressed air inlet (3) is connected with a sterile air conveying pipe (6), and the sterile air conveying pipe (6) is vertically connected with a plurality of air distributors (12); a feed inlet (7) and a sampling port (8) are arranged at the middle upper part of the fermentation tank (1), a cooling water jacket (9) is arranged at the middle part of the fermentation tank, and a cooling water inlet (11) and a cooling water outlet (10) are arranged on the cooling water jacket (9); fermentation cylinder (1) bottom is provided with drain hole (13), fermentation cylinder (1) below is provided with support (14).
2. The apparatus for culturing Bacillus by liquid fermentation according to claim 1, wherein the number of the air distributors (12) is 5 to 10, and the air distributors (12) are arranged in a direction perpendicular to the sterile air supply pipe (6), and the air distributors (12) are arranged at equal intervals in the direction of the sterile air supply pipe (6).
3. The apparatus for culturing Bacillus by liquid fermentation according to claim 1, wherein the air distributor (12) has an average distribution of pores with a diameter of 2-5mm in each direction.
4. The apparatus for culturing bacillus by liquid fermentation according to claim 1, wherein the angle between the manhole (2) and the sterile air delivery pipe (6) is 30 ° to 50 °.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920226110.8U CN209872937U (en) | 2019-02-23 | 2019-02-23 | Liquid fermentation culture bacillus device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920226110.8U CN209872937U (en) | 2019-02-23 | 2019-02-23 | Liquid fermentation culture bacillus device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209872937U true CN209872937U (en) | 2019-12-31 |
Family
ID=68953078
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920226110.8U Expired - Fee Related CN209872937U (en) | 2019-02-23 | 2019-02-23 | Liquid fermentation culture bacillus device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209872937U (en) |
-
2019
- 2019-02-23 CN CN201920226110.8U patent/CN209872937U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191231 |