CN207468632U - A kind of installation for fermenting for solid fermentation bacillus subtilis - Google Patents
A kind of installation for fermenting for solid fermentation bacillus subtilis Download PDFInfo
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- CN207468632U CN207468632U CN201721254746.0U CN201721254746U CN207468632U CN 207468632 U CN207468632 U CN 207468632U CN 201721254746 U CN201721254746 U CN 201721254746U CN 207468632 U CN207468632 U CN 207468632U
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Abstract
The utility model discloses a kind of installation for fermenting for solid fermentation bacillus subtilis, including fixed frame, several blowing plates, several agitating devices and sprinkling water installations, wherein, the fixed frame by fixed column and several support bar groups into；The blowing plate is made of left plate and right plate, left plate, right plate outer ledge be equipped with baffle；Left plate, right plate are equipped with multiple leakage holes；Agitating device is made of driving motor, motor-driven agitating shaft driven and several stirring handgrips for being arranged on agitating shaft；Sprinkling water installations are arranged on the back side of left plate, right plate by water pipe and several sprinkler compositions being arranged on water pipe, water pipe.The utility model is stirred supplementary material using physical method, ventilates, moisturizing, temperature control, not only reduce production cost, but also improve capacity efficiency, make supplementary material mixing more uniform, increase ventilatory capacity, can ensure the effect of fermentation, it is simple in structure, it is easy to use, save a large amount of manpower and materials.
Description
Technical field
The utility model is related to a kind of installation for fermentings for solid fermentation bacillus subtilis.
Background technology
Bacillus subtilis (Bacillus subtilis) is one kind of bacillus, Gram-positive, aerobic bacteria,
Without pod membrane, peritrichous can move.It is mould that bacillus subtilis thalline generates subtilin, polymyxins, system in growth course
Rhzomorph, gramicidins isoreactivity substance, these active materials have significantly the conditioned pathogen of pathogenic bacteria or autogenous infection
Inhibiting effect, while bacillus subtilis can also discharge some active materials beneficial to plant growth, stimulate the growth of plant.
At present for the fermentation of bacillus subtilis, generally existing viable count and gemma rate is relatively low asks in production process
Topic, general spore content is 5.0 × 109Cfu/mL substantially reduces bacterium hereinafter, the thalline of no gemma can be dead in a short time
The activity and quality stability of agent；Meanwhile the extraction energy consumption of liquid fermentation later stage viable bacteria is larger, a large amount of waste liquids is generated, to environment
Certain pollution is caused, and the research of solid fermentation is in laboratory stage mostly, mechanization industrial production does not have ripe case,
Solid fermentation apparatus of the prior art cannot meet the fermentation needs of bacillus subtilis, and sterilization and automation condition compare
It is low, need a large amount of manpower and materials.
Invention content
For the above-mentioned prior art, it is the sterilization of realization fermentation process, mechanization, automation, the utility model provides
A kind of installation for fermenting and fermentation process for solid fermentation bacillus subtilis.
The utility model is achieved through the following technical solutions：
A kind of installation for fermenting for solid fermentation bacillus subtilis including fixed frame, several blowing plates, several stirs
Device and sprinkling water installations are mixed, wherein,
The fixed frame is made of at least two fixed columns or fixed frame and several supporting rods or supporting rack, Gu
Fixed column or fixed frame fix on the ground vertically, and supporting rod or supporting rack are horizontal positioned, and the both ends of supporting rod or supporting rack are with consolidating
Fixed column or fixed frame are fixedly connected, and several supporting rods or supporting rack are arranged in parallel from top to bottom, positioned at undermost supporting rod
Or the lower section of supporting rack be equipped with it is several go out material frame；
The blowing plate is made of left plate and right plate, and left plate, right plate are hinged (logical with supporting rod or supporting rack
Cross hinge connection), left plate, right plate outer ledge be equipped with baffle (in application, preventing fermentation materials from left plate, right side
It trickles down at the edge of plate)；Left plate, right plate are equipped with the latch structure to cooperate (when left plate, right plate are horizontal positioned
When, it is fixed by latch structure, fermentate can be placed in plate face at this time；When opening bolt, due to gravity, left side
Plate, right plate hang down naturally, and fermentate thereon is fallen) or：Left plate, right plate be equipped with latch structure, fixed column or
Fixed frame (when left plate, right plate are horizontal positioned, passes through latch structure and fixation equipped with latch structure with matching
Fermentate, can be placed in plate face by column or fixed frame connection at this time；When opening bolt, due to gravity, left plate, right side
Plate hangs down naturally, and fermentate thereon is fallen)；The left plate, right plate are equipped with multiple leakage holes (in fermentation process
The distance between ventilation), diameter≤2mm of leakage hole, adjacent leakage hole >=2mm；
The agitating device is by driving motor, motor-driven agitating shaft driven and be arranged on agitating shaft several stir
Handgrip composition is mixed, wherein, agitating shaft is placed vertically and (left plate, right plate are equipped with corresponding across left plate or right plate
Hole is for passing through).During practical application, agitating device can be fixed on roof indoors or fix support by special supporting rack.
During work, driving motor is connected with power supply, drives agitating shaft rotation, and then the rotation of stirring handgrip is driven to stir material
It mixes.
The sprinkling water installations by water pipe and several sprinkler compositions for being arranged on water pipe, wherein, water pipe is arranged on left side
Plate, right plate the back side (during concrete application, to economize on resources, positioned at the blowing back of the bottom can be not provided with spray water dress
It puts)；In use, water pipe is connect in real time with tap, sterile water can be directly inputted, also can also set and be sterile filtered in water pipe
Device is filtered tap water.
Preferably, the fixed frame, agitating shaft, stirring handgrip are made of stainless steel material, the left plate, right side
Plate goes out material frame and is made of polytetrafluoroethylene material.
Preferably, for 40cm, length 8m, thickness 5mm, the height of baffle is the width of the left plate, right plate
10cm。
Preferably, the blowing plate has 2~4 layers, during concrete application, is flexibly selected according to chamber height etc..
Preferably, the distance between the blowing plate, neighbouring blowing plate are 80cm.
Preferably, the stirring handgrip can be paddle or robot gripper, and structure can be：Including
Four parts, each part include 5 fingers, during work, stirring handgrip can be moved up and down along central shaft (when not working, stirring
Handgrip is parked in the top of every layer of blowing plate, is not contacted with fermentation materials), to achieve the effect that preferably to stir material.
Preferably, nozzle is the round nozzle in 8 holes, the sprayed fiber concrete of nozzle for the distance between 17cm, adjacent nozzle >=
33cm (is not installed, prevent stirring handgrip waterlogged damage) at agitating shaft.
Preferably, further include top plate, top plate is provided at the top of the blowing plate of top layer, top plate both ends and fixed column or
Fixed frame is fixedly connected, and the top plate back side (lower surface) (sprays the blowing plate for being located at top layer equipped with sprinkling water installations
Water)；Driving motor can be fixed on top plate.Or：To economize on resources, can be not provided in the blowing plate of top layer leakage hole,
Baffle.
A kind of solid fermentation method of bacillus subtilis：Solid fermentation zymotic fluid is mixed with solid fermentation culture medium
Mixing (inoculum concentration 10%) tiles (thickness 4cm), ferments, fermentation condition is：37 DEG C of temperature cultivates 72h, at interval of 12h
Moisturizing is simultaneously stirred 1 time (moisturizing to keep the humidity of culture 60%)；After fermentation, tunning is obtained, removes fermentation production
Object, ventilation are dried, and are crushed, you can use or carry out following process.
Further, the method that solid fermentation is carried out using above device：By solid fermentation zymotic fluid and solid fermentation
Culture medium mixing mixing (inoculum concentration 10%), is laid in the blowing plate fixed (thickness 4cm), ferments, fermentation condition
For：37 DEG C of temperature cultivates 72h, at interval of 12h moisturizings and stirs 1 time (moisturizing to keep the humidity of culture 60%) and (uses
Water installations moisturizing is sprayed, using the stirring of stirring handgrip)；After fermentation, left plate, right plate are opened, tunning is fallen into out
Charging basket removes out charging basket, and ventilation is dried, and crushes, you can uses or carry out following process.
The solid fermentation culture medium is by material-water ratio 1 by solid content and water:1.1 compositions；The solid content, be by
The component composition of following weight percent：Bean cake powder 9%, powdered rice hulls 10%, glucose 0.5%, ammonium sulfate 0.5%, sulfuric acid
Magnesium 0.1%, manganese sulfate 0.05%, surplus are wheat bran.
Further, the solid fermentation zymotic fluid, is prepared by the following method to obtain：
(1) prepared by primary seed solution：By the bacillus subtilis inclined-plane of 4 DEG C of preservations, it is inoculated in LB fluid nutrient medium (yeast
Powder 0.5%, tryptone 1%, sodium chloride 1%) in, rotating speed 200rpm/min, 37 DEG C are cultivated 17h, the withered grass bud activated
Spore bacillus primary seed solution；
(2) prepared by secondary seed solution：The bacillus subtilis of activation is inoculated in 1000mLLB liquid by 2% inoculum concentration
In culture medium, shaking flask culture, 37 DEG C of temperature, rotating speed 200rpm/min cultivate 17h, obtain secondary seed solution；
(3) 50L fermentor liquids culture：The seed liquor that step (2) obtains is inoculated in 50L fermentation tanks and (includes LB liquid
Culture medium) in, inoculum concentration 2%, 37 DEG C, rotating speed 200rpm/min of temperature cultivates 17h, obtains solid fermentation zymotic fluid.
The utility model is stirred supplementary material using physical method, ventilates, moisturizing, temperature control, both reduced and be produced into
This, and capacity efficiency is improved, make supplementary material mixing more uniform, increase ventilatory capacity, can ensure the effect of fermentation, structure letter
It is single, it is easy to use, save a large amount of manpower and materials.The installation for fermenting of the utility model, clever structure, in fermentation process, fermentate
Ventilation, moisturizing, the stirring of material can facilitate realization, after fermentation, can rapidly collect fermentation materials, use is very square
Just, it can be applicable.
The content of above-mentioned not detailed description description, is existing technology, such as agitating device, spray water in the prior art
Device, latch structure, can market be commercially available or realize by conventional methods.
Description of the drawings
Fig. 1：The structure schematic diagram of the installation for fermenting for solid fermentation bacillus subtilis of the utility model.
Fig. 2：The structure schematic diagram (bottom view) of blowing plate.
Wherein, 1, fixed column；2nd, supporting rod；3rd, left plate；4th, right plate；5th, go out material frame；6th, baffle；7th, latch structure；
8th, leakage hole；9th, driving motor；10th, agitating shaft；11st, handgrip is stirred；12nd, water pipe；13rd, nozzle.
Note：Above structure figure is only description scheme, convenient for those skilled in the art understand that drawn, not representing practical ruler
Very little, relative distance and ratio, during practical application, size, dimension scale, model, specification, layout, each structure of involved structure
Between relative distance etc., flexibly selected according to actual conditions by manufacturer.
Specific embodiment
With reference to embodiment, the present invention is further illustrated.
Involved instrument, reagent, material etc., are existing in the prior art unless otherwise noted in following embodiments
Conventional instrument, reagent, material etc., can be obtained by regular commercial sources.Involved experimental method in following embodiments, inspection
Survey method etc. is existing routine experiment method, detection method etc. in the prior art unless otherwise noted.
A kind of installation for fermenting for solid fermentation bacillus subtilis including fixed frame, 2 layers of blowing plate, several stirs
Device and sprinkling water installations are mixed, as shown in Figure 1, wherein,
The fixed frame is made of two fixed columns 1 and 2 supporting rods 2, and fixed column 1 fixes on the ground vertically,
Supporting rod 2 is horizontal positioned, and the both ends of supporting rod 2 are fixedly connected with fixed column 1, and 2 supporting rods 2 are arranged in parallel from top to bottom, position
In lower floor supporting rod 2 lower section be equipped with it is several go out material frame 5；
The blowing plate is made of left plate 3 and right plate 4, as shown in Fig. 2, left plate 3, right plate 4 with supporting rod 2
Hinged (passing through hinge connection), left plate 3, right plate 4 outer ledge be equipped with baffle 6 (in application, preventing fermentation materials from a left side
Side plate 3, right plate 4 edge trickle down)；Left plate 3, right plate 4 be equipped with cooperate latch structure 7 (when left plate 3,
It when right plate 4 is horizontal positioned, is fixed by latch structure 7, fermentate can be placed in plate face at this time；When opening bolt, by
In gravity, left plate 3, right plate 4 hang down naturally, and fermentate thereon is fallen)；On the left plate 3, right plate 4
Equipped with multiple leakage holes 8 (be arranged in a manner of rows of left plate 3, right plate 4 on) (for ventilating in fermentation process), leakage hole 8
Diameter≤2mm, the distance between adjacent leakage hole 8 >=2mm；
The agitating shaft 10 and be arranged on agitating shaft 10 that the agitating device is driven by driving motor 9, motor driven 9
Several stirring handgrips 11 form, wherein, agitating shaft 10 is placed and passes through left plate 3 or right plate 4 (left plate 3, right plate vertically
4 are equipped with corresponding hole for passing through).During practical application, agitating device can be fixed on roof indoors or on top plate, Huo Zheyou
Special supporting rack fixes support.During work, driving motor 9 is connected with power supply, and agitating shaft 10 is driven to rotate, and then drives stirring
Handgrip 11 rotates to be stirred material.
The sprinkling water installations are made of water pipe 12 and several nozzles 13 being arranged on water pipe 12, wherein, water pipe 12 is set
Put that (during concrete application, to economize on resources, the blowing back positioned at the bottom can not be set at the back side of left plate 3, right plate 4
Put sprinkling water installations)；In use, water pipe 12 is connect in real time with tap, sterile water can be directly inputted, also can also be set in water pipe
Sterilizing filter is put, tap water is filtered.
The fixed frame, agitating shaft 10, stirring handgrip 11 be made of stainless steel material, the left plate 3, right plate 4,
Go out material frame 5 to be made of polytetrafluoroethylene material.
The left plate 3, right plate 4 width for 40cm, length 8m, thickness 5mm, the height of baffle is 10cm.
The distance between neighbouring blowing plate is 80cm.
The stirring handgrip 11 can be paddle or robot gripper.
The nozzle 13 is the round nozzle in 8 holes, the sprayed fiber concrete of nozzle for the distance between 17cm, adjacent nozzle >=
33cm (is not installed, prevent stirring handgrip waterlogged damage) at agitating shaft.
Top plate is further included, top plate is provided at the top of the blowing plate of top layer, top plate both ends and fixed column or fixed frame
It is fixedly connected, the top plate back side (lower surface) to the blowing plate for being located at top layer equipped with sprinkling water installations (carrying out spray water)；Driving
Motor can be fixed on top plate.
The solid fermentation of 1 bacillus subtilis of embodiment
It is fermented using above-mentioned installation for fermenting, step is as follows：
(1) bacillus subtilis of 4 DEG C of preservations is inoculated in fluid nutrient medium (dusty yeast 0.25g, the tryptone of sterilizing
0.5g, sodium chloride 0.5g, water 50mL, 121 DEG C, sterilizing 20min) in, 37 DEG C, 200rpm/min cultivate 17h, obtain withered grass bud
Spore bacillus activates bacterium solution.
(2) bacterium solution of 16mL activation is drawn with liquid-transfering gun, is inoculated in 4 1000mL shaking flasks, each Shake flask medium dress
Liquid measure is 200ml, and shaking table culture condition is：37 DEG C, 200rpm/min cultivate 17h.
(3) seed liquor obtained in the previous step is inoculated in 50L fermentation tanks, culture medium liquid amount is 35L, connects bacterium amount
700mL, fermentation condition are：37 DEG C, ventilatory capacity 1:1st, rotating speed 200rpm/min, fermented and cultured 17h obtain solid fermentation bacteria used thereby
Liquid；
(4) 15L zymotic fluids are taken, add in 150L aqua sterilisas, are inoculated in the solid medium of 150kg sterilizings, are put down after mixing
It is layered on left plate, on right plate.Solid fermentation Medium Proportion：Wheat bran 119.775kg, bean cake powder 13.5kg, powdered rice hulls 15kg,
Glucose 0.75kg, ammonium sulfate 0.75kg, magnesium sulfate 0.15kg, manganese sulfate 0.075kg；The sterilising conditions of culture medium are：121
DEG C, 25min, fermentation condition is：Material thickness 4cm, 37 DEG C, 72h, stirring 1 time/12h (using stirring handgrip stirring), moisturizing 1
Secondary/12h (using the moisturizing of sprinkling water installations).Nutrient agar panel counts viable count 3.2 × 1010Cfu/g, malachite green gemma dye
Color, oil mirror observation gemma rate 93.2%.
(5) left plate, right plate are opened, it is made to hang down naturally, solid fermentation product enters charging basket, removes out charging basket and leads to
Wind dries, and crushes, obtains producing bacillus subtilis product.
The solid fermentation of 2 bacillus subtilis of embodiment
It is fermented using above-mentioned installation for fermenting, step is as follows：
(1) bacillus subtilis of 4 DEG C of preservations is inoculated in fluid nutrient medium (dusty yeast 0.25g, the tryptone of sterilizing
0.5g, sodium chloride 0.5g, water 50mL, 121 DEG C, sterilizing 20min) in, 37 DEG C, 200rpm/min cultivate 17h, obtain withered grass bud
Spore bacillus activates bacterium solution.
(2) bacterium solution of 16mL activation is drawn with liquid-transfering gun, is inoculated in 4 1000mL shaking flasks, each Shake flask medium dress
Liquid measure is 200ml, and shaking table culture condition is：37 DEG C, 200rpm/min cultivate 17h.
(3) seed liquor obtained in the previous step is inoculated in 50L fermentation tanks, culture medium liquid amount is 35L, connects bacterium amount
700mL, fermentation condition are：37 DEG C, ventilatory capacity 1:1st, rotating speed 200rpm/min, fermented and cultured 17h obtain solid fermentation bacteria used thereby
Liquid；
(4) 30L zymotic fluids are taken, add in 300L aqua sterilisas, are inoculated in the solid medium of 300kg sterilizings, are put down after mixing
It is layered on left plate, on right plate.Solid fermentation Medium Proportion：Wheat bran 239.55kg, bean cake powder 27kg, powdered rice hulls 30kg, Portugal
Grape sugar 1.5kg, ammonium sulfate 1.5kg, magnesium sulfate 0.3kg, manganese sulfate 0.15kg；The sterilising conditions of culture medium are：121℃、
25min, fermentation condition are：Material thickness 4cm, 37 DEG C, 72h, stirring 1 time/12h, 1 time/12h of moisturizing.Nutrient agar panel meter
Number viable count 3.5 × 1010Cfu/g, malachite green spore staining, oil mirror observation gemma rate 93.6%.
(5) left plate, right plate are opened, it is made to hang down naturally, solid fermentation product enters charging basket, removes out charging basket and leads to
Wind dries, and crushes, obtains producing bacillus subtilis product.
Claims (7)
1. a kind of installation for fermenting for solid fermentation bacillus subtilis, it is characterised in that：Including fixed frame, several blowings
Plate, several agitating devices and sprinkling water installations, wherein,
The fixed frame is made of at least two fixed columns or fixed frame and several supporting rods or supporting rack, fixed column
Or fixed frame fixes on the ground vertically, supporting rod or supporting rack are horizontal positioned, the both ends and fixed column of supporting rod or supporting rack
Or fixed frame is fixedly connected, several supporting rods or supporting rack are arranged in parallel from top to bottom, positioned at undermost supporting rod or branch
The lower section of support be equipped with it is several go out material frame；
The blowing plate is made of left plate and right plate, and left plate, right plate are hinged with supporting rod or supporting rack, left side
Plate, right plate outer ledge be equipped with baffle；Left plate, right plate are equipped with the latch structure to cooperate, when left plate, the right side
It when side plate is horizontal positioned, is fixed by latch structure, fermentate can be placed in plate face at this time；When opening bolt, due to weight
Power acts on, and left plate, right plate hang down naturally, and fermentate thereon is fallen；Or：Left plate, right plate are equipped with bolt knot
Structure, fixed column or fixed frame are equipped with latch structure with matching；The left plate, right plate are equipped with multiple leakage holes,
The distance between diameter≤2mm of leakage hole, adjacent leakage hole >=2mm；
The agitating device is grabbed by driving motor, motor-driven agitating shaft driven and several stirrings for being arranged on agitating shaft
Hand forms, wherein, agitating shaft is placed and passes through left plate or right plate vertically；
The sprinkling water installations by water pipe and several sprinkler compositions for being arranged on water pipe, wherein, water pipe is arranged on left plate, the right side
The back side of side plate.
2. the installation for fermenting according to claim 1 for solid fermentation bacillus subtilis, it is characterised in that：The left side
Side plate, right plate width for 40cm, length 8m, thickness 5mm, the height of baffle is 10cm.
3. the installation for fermenting according to claim 1 for solid fermentation bacillus subtilis, it is characterised in that：It is described to put
Flitch has 2~4 layers, and the distance between neighbouring blowing plate is 80cm.
4. the installation for fermenting according to claim 1 for solid fermentation bacillus subtilis, it is characterised in that：It is described to stir
It is paddle or robot gripper to mix handgrip.
5. the installation for fermenting according to claim 1 for solid fermentation bacillus subtilis, it is characterised in that：The spray
Head is the round nozzle in 8 holes, and the sprayed fiber concrete of nozzle is the distance between 17cm, adjacent nozzle >=33cm.
6. the installation for fermenting according to claim 1 for solid fermentation bacillus subtilis, it is characterised in that：It is described solid
Determine frame, agitating shaft, stirring handgrip to be made of stainless steel material, the left plate, right plate go out material frame by polytetrafluoroethylene (PTFE) material
Material is made.
7. the installation for fermenting according to claim 1 for solid fermentation bacillus subtilis, it is characterised in that：It further includes
Top plate, top plate are provided at the top of the blowing plate of top layer, and top plate both ends are fixedly connected with fixed column or fixed frame, the top plate back of the body
Face is equipped with sprinkling water installations；Driving motor is fixed on top plate.
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| CN201721254746.0U CN207468632U (en) | 2017-09-27 | 2017-09-27 | A kind of installation for fermenting for solid fermentation bacillus subtilis |
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| CN201721254746.0U CN207468632U (en) | 2017-09-27 | 2017-09-27 | A kind of installation for fermenting for solid fermentation bacillus subtilis |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107475091A (en) * | 2017-09-27 | 2017-12-15 | 山东劲牛生物科技股份有限公司 | A kind of installation for fermenting and fermentation process for solid fermentation bacillus subtilis |
-
2017
- 2017-09-27 CN CN201721254746.0U patent/CN207468632U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107475091A (en) * | 2017-09-27 | 2017-12-15 | 山东劲牛生物科技股份有限公司 | A kind of installation for fermenting and fermentation process for solid fermentation bacillus subtilis |
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| GR01 | Patent grant | ||
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Address after: 250101 room 1001, block B, building 1, Jinan pharmaceutical Valley R & D platform area, No.1, north section of Gangxing Third Road, comprehensive bonded zone, high tech Zone, Jinan City, Shandong Province Patentee after: Shandong Jinniu Group Co.,Ltd. Address before: 250101 room 1001, block B, building 1, Jinan pharmaceutical Valley R & D platform area, No.1, north section of Gangxing Third Road, comprehensive bonded zone, high tech Zone, Jinan City, Shandong Province Patentee before: SHANDONG JINNIU BIOTECHNOLOGY Co.,Ltd. |
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| CP01 | Change in the name or title of a patent holder |