CN109439515B - Rotary ball type microorganism two-stage expanding culture device and method - Google Patents
Rotary ball type microorganism two-stage expanding culture device and method Download PDFInfo
- Publication number
- CN109439515B CN109439515B CN201811554121.5A CN201811554121A CN109439515B CN 109439515 B CN109439515 B CN 109439515B CN 201811554121 A CN201811554121 A CN 201811554121A CN 109439515 B CN109439515 B CN 109439515B
- Authority
- CN
- China
- Prior art keywords
- valve
- power
- power end
- ball type
- pipeline
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 6
- 238000005507 spraying Methods 0.000 claims abstract description 34
- 239000000654 additive Substances 0.000 claims abstract description 23
- 230000000996 additive effect Effects 0.000 claims abstract description 23
- 230000007246 mechanism Effects 0.000 claims abstract description 20
- 230000005540 biological transmission Effects 0.000 claims abstract description 19
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000001301 oxygen Substances 0.000 claims abstract description 17
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 17
- 238000005070 sampling Methods 0.000 claims abstract description 13
- 238000002347 injection Methods 0.000 claims abstract description 8
- 239000007924 injection Substances 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 13
- 230000000813 microbial effect Effects 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 238000012136 culture method Methods 0.000 claims description 5
- 239000007789 gas Substances 0.000 claims description 5
- 238000007599 discharging Methods 0.000 claims description 4
- 230000003321 amplification Effects 0.000 claims description 3
- 238000005520 cutting process Methods 0.000 claims description 3
- 210000004907 gland Anatomy 0.000 claims description 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 3
- 238000005086 pumping Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 239000011259 mixed solution Substances 0.000 claims 2
- 238000012364 cultivation method Methods 0.000 claims 1
- 230000007774 longterm Effects 0.000 claims 1
- 238000005096 rolling process Methods 0.000 claims 1
- 241000894006 Bacteria Species 0.000 description 3
- 238000005265 energy consumption Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 239000011344 liquid material Substances 0.000 description 2
- 238000010008 shearing Methods 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000193171 Clostridium butyricum Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000010907 mechanical stirring Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a rotary ball type microorganism two-stage expanding culture device, which comprises a rotary ball type expanding culture chamber, a power transmission mechanism and a feeding injection oxygen supply mechanism; the rotary ball type expanding culture chamber comprises a hollow ball, and the left end of the rotary ball type expanding culture chamber is provided with a power end; the right end is provided with a spraying end; the bottom is provided with a discharge valve, and the rotary ball type expanding culture chamber is also provided with an exhaust pipe; the power transmission mechanism comprises a large transmission turbine, a power worm and a power end base; the feeding and spraying oxygen supply mechanism comprises a pipeline, a cross pipe, a feeding valve, a vacuum injection pump, an electromagnetic directional valve, a constant-temperature electromagnetic heater, a magnetic circulating pump, an additive valve, an additive batching tank, a discharge port and a sampling pipe. The device and the method provided by the invention can make the environment more suitable for the secondary propagation of microorganisms, greatly improve the yield and save energy.
Description
Technical Field
The invention belongs to the technical field of microorganism culture, and particularly relates to a rotary ball type microorganism two-stage expanding culture device and a rotary ball type microorganism two-stage expanding culture method.
Background
In the field of microorganisms, the culture and proliferation of strains are often involved, strains are proliferated in a proper culture medium under a certain temperature condition to expand the number, and the use requirements of the strains in the fields of human medicine, veterinary medicine, feed, cultivation and the like are met. Especially for bacillus, such as bacillus subtilis, bacillus licheniformis, bacillus cereus, clostridium butyricum and the like, fermentation and multiplication are needed in a fermentation tank, so that the number of live bacteria is obviously increased. The secondary amplification of the microorganism is to obtain pure strains, and the bacteria of each colony are divided and propagated by the same bacteria. Their genetic material is essentially the same.
At present, the strain expanding culture in the prior art adopts a vertical fixed tank, oxygen in the tank is not uniformly contacted with the strain, the strain is easily damaged under the shearing action generated under the mechanical stirring, the energy consumption is higher, the power of the expanding culture tank of about 2 cubic meters is about 5kw, the cost control is extremely unfavorable, and enterprises lose market competitiveness.
Disclosure of Invention
Aiming at the problems, the invention provides a rotary ball type microorganism two-stage propagation device and a method, wherein a rotary hollow ball (the volume is 5-40 cubic, the outer layer is insulated) is utilized to adjust the pH value, the temperature, the strain quantity and the balanced nutrient source of the liquid material in the rotary ball, the correct propagation time is set, and the strain liquid material is fully contacted with oxygen under the oxygen supply and circulation action of a hydraulic ejector and the low-speed rotation of the rotary ball, so that the healthy strain with geometric multiple is obtained.
The expanding culture device is suitable for expanding culture of aerobic microorganism strains.
The technical scheme of the invention is as follows:
a rotary ball type microorganism two-stage expanding culture device comprises a rotary ball type expanding culture chamber, a power transmission mechanism and a feeding injection oxygen supply mechanism.
The rotary ball type expanding culture chamber comprises a hollow ball, a manhole is arranged above the hollow ball, a power end hollow shaft, a power end outer bearing and a power end inner bearing are arranged at the left end of the hollow ball, the power end inner bearing is arranged inside the power end outer bearing, and the power end hollow shaft is fixed in the middle of the power end inner bearing; the right end is provided with a spraying end hollow shaft, a spraying end outer sealing gland and a spraying end inner bearing, the spraying end inner bearing is arranged inside the spraying end outer bearing, and the spraying end hollow shaft is fixed in the middle of the spraying end inner bearing; the bottom is provided with a discharge valve, the periphery of the discharge valve is also provided with a plurality of lifting stirring barrels, and the rotary ball type expanding culture chamber is also provided with an exhaust pipe communicated with the inside and the outside.
The power transmission mechanism comprises a large transmission turbine, a power worm and a power end base, wherein the power end base is of an L-shaped structure, the power worm is arranged above the transverse surface of the bottom of the power end base, the axis of the large transmission turbine is arranged on an outer power end bearing, and the bottom of the outer power end bearing is arranged on the power end base.
The feeding and spraying oxygen supply mechanism comprises a pipeline, a cross pipe, a feeding valve, a vacuum injection pump, an electromagnetic directional valve, a constant-temperature electromagnetic heater, a magnetic circulating pump, an additive valve, an additive batching tank, a discharge port and a sampling pipe; the pipeline includes 1 section straight tube section, 1 section segmental arc and 1 section broken line section, broken line section and segmental arc one end lug connection, the other end passes through the cross union coupling, the cross union left end passes the injection end quill shaft and gets into the clean shot, be located the inboard pipeline end of clean shot and export as the sprayer, the sprayer export downward sloping, be equipped with vacuum jet pump and solenoid directional valve on the cross union right side in proper order, solenoid directional valve bottom is the bin outlet, be equipped with down the sampling tube on the pipeline on solenoid directional valve right side, top-down is equipped with additive batching jar in proper order on the broken line section, magnetic circulating pump and constant temperature electromagnetic heater, the other joint of additive batching jar is on the broken line section, and it has the additive valve, magnetic circulating pump has the pipeline pump valve.
Furthermore, one end of the exhaust pipe penetrates through the hollow shaft at the power end and extends into the hollow ball, the exhaust pipe in the hollow ball is bent upwards after entering the hollow ball and reaches the position near a population, the bent shape of the exhaust pipe is matched with the arc shape of the inner wall of the air ball, the other end of the exhaust pipe extends out of the hollow shaft at the power end for a section and then is erected upwards, a fixed upright column is arranged, the upper end of the fixed upright column is connected to the exhaust pipe, and the lower end of the fixed upright column is fixed on the base at the power end.
A rotary ball type microorganism two-stage expanding culture method comprises the following steps:
1. and closing the manhole, placing the electromagnetic directional valve in the pipeline passage, and closing the sampling pipe, the pipeline pump valve, the additive valve and the discharge valve.
2. And opening the feed valve, and pumping the strain culture expanding mixed liquid into the cross pipe to enable the mixed liquid to enter the hollow ball from the material inlet and the material outlet of the cross pipe along the gap between the inner wall of the cross pipe and the outer wall of the vacuum jet pump.
3. The adding amount of the strain culture expanding mixed liquid is controlled at the upper part of the hollow shaft at the power end (the volume of the water-gas mixed liquid of the hollow ball is increased due to oxygen brought by negative pressure when the vacuum jet pump is started), and the liquid level of the water-gas mixed liquid must not exceed the excess air inlet of the exhaust port in principle.
4. The feed valve is closed and the magnetic pipeline pump valve is opened.
5. Starting the magnetic circulating pump to make the mixed liquid of strains in the working chamber sprayed into the working chamber together with oxygen through the vacuum jet pump and the material inlet and outlet of the cross pipe, and then starting the transmission mechanism to make the whole hollow ball enter a rotation mode and enter a normal operation state (the specific speed is set according to different microbial strains).
6. Different expanding culture time is selected according to different microbial strains, and after a detection sample (sampled and detected by a sampling tube) is qualified, the electromagnetic directional valve is placed in a discharge state by cutting off a passage of the vacuum ejector, so that the material is lifted out from a discharge port.
7. If the machine is stopped for a long time or different propagation strains are replaced, the discharge valve can be opened to completely discharge the materials.
In the steps, because the operation mechanisms are fewer and the speed is more stable, the energy consumption is extremely low and is about one fifth of the energy consumption in the prior art.
The invention has the beneficial effects that:
1. by adopting the expanding culture method, the whole process has no dead angle, a large amount of micro bubbles are generated in the working chamber, the microorganisms are fully contacted with oxygen, the expanding culture period is short, the strains are more robust, and the discharging is clean.
2. The jet circulation stirring and the rotary ball type self-stirring are adopted, so that no other shearing force is generated except the impact force of water power on the microbial strains, and the growth of the microorganisms in a healthy environment is facilitated.
3. The rotating speed of the sphere is low, even if the culture expanding device is more than 20 cubic meters, the total power does not exceed 10kw, and the energy waste brought by the prior art is greatly overcome.
4. The required equipment has simple structure and low cost, and is beneficial to large-scale production.
Drawings
Fig. 1 is a schematic structural sectional view of the present invention.
In the figure:
01 power end bearing, 02 transmission big turbine, 03 power end hollow shaft, 04 exhaust pipe, 05 manhole, 06 hollow sphere, 07 spraying end hollow shaft, 08 spraying end outer bearing, 09 feed valve, 10 cross pipe, 11 sterile air suction inlet, 12 vacuum jet pump, 13 electromagnetic directional valve, 14 discharge outlet, 15 sampling pipe, 16 pipeline, 17 constant temperature electromagnetic heater, 18 magnetic circulating pump, 19 pipeline pump valve, 20 additive valve, 21 additive batching tank, 22 spraying end base, 23 spraying end inner bearing, 24 ejector outlet, 25 cross pipe material inlet and outlet, 26 discharge valve, 27 lifting stirring barrel, 28 power end inner bearing, 29 power end base, 30 power worm, 31 fixed column.
Detailed Description
The invention is further illustrated with reference to the following figures and examples.
As shown in figure 1, a rotary ball type microorganism two-stage culture spreader comprises a rotary ball type culture spreading chamber, a power transmission mechanism and a feeding, spraying and oxygen supplying mechanism.
The rotary ball type expanding culture chamber comprises a hollow ball 6, a manhole 5 is arranged above the hollow ball 6, a power end hollow shaft 3, a power end outer bearing 1 and a power end inner bearing 28 are arranged at the left end, the power end inner bearing 28 is arranged inside the power end outer bearing 1, and the power end hollow shaft 3 is fixed in the middle of the power end inner bearing 28; the right end is provided with a spraying end hollow shaft 7, a spraying end outer sealing gland and a spraying end inner bearing 23, the spraying end inner bearing 23 is arranged inside the spraying end outer bearing 8, and the spraying end hollow shaft 7 is fixed in the middle of the spraying end inner bearing 23; the bottom is provided with a discharge valve 26, a plurality of (for example 6) lifting stirring barrels 27 are arranged around the discharge valve 26, and the rotary ball type expanding culture chamber is also provided with an exhaust pipe 4 communicating the inside and the outside.
The power transmission mechanism comprises a large transmission turbine 2, a power worm 30 and a power end base 29, wherein the power end base 29 is of an L-shaped structure, the power worm 30 is installed above the cross surface of the bottom of the power end base, the axis of the large transmission turbine 2 is installed on an outer power end bearing 1, and the bottom of the outer power end bearing 1 is installed on the power end base 29.
The feeding and spraying oxygen supply mechanism comprises a pipeline 16, a cross pipe 10, a feeding valve 9, a vacuum spraying pump 12, an electromagnetic directional valve 13, a constant temperature electromagnetic heater 17, a magnetic circulating pump 18, an additive valve 20, an additive batching tank 21, a discharge port 14 and a sampling pipe 15; the pipeline 16 comprises 1 straight pipeline section, 1 arc section and 1 broken pipeline section, one end of the broken pipeline section is directly connected with one end of the arc section, the other end of the broken pipeline section is connected with the other end of the arc section through a cross pipe 10, the left end of the cross pipe 10 penetrates through a hollow shaft 7 at the spraying end and enters the hollow ball 6, at the end of the pipe 16 inside the hollow sphere 6, which is the ejector outlet 24, the ejector outlet 24 is inclined downwards, a vacuum injection pump 12 and an electromagnetic directional valve 13 are sequentially arranged on the right side of the cross pipe 10, a discharge opening 14 is arranged at the bottom of the electromagnetic directional valve 13, a downward sampling pipe 15 is arranged on a pipeline 16 on the right side of the electromagnetic directional valve 13, an additive dosing tank 21, a magnetic circulating pump 18 and a constant temperature electromagnetic heater 17 are sequentially arranged on the broken line section from top to bottom, the additive dosing tank 21 is connected to the broken line section by the side, and it has an additive valve 20, a magnetic circulating pump 18 has a pipe pump valve 19, and a broken line section of the pipe 16 is fixed on a spray end base 22.
One end of the exhaust pipe 4 penetrates through the hollow shaft 3 at the power end and extends into the hollow ball 6, the exhaust pipe 4 in the hollow ball 6 is bent upwards after entering the hollow ball 6 and reaches the position near the population, the bent shape of the exhaust pipe is matched with the arc shape of the inner wall of the air ball, the other end of the exhaust pipe extends out of the hollow shaft 3 for a section and then is erected upwards, a fixed upright column 31 is arranged, the upper end of the fixed upright column 31 is connected to the exhaust pipe 4, and the lower end of the fixed upright column is fixed on a base 29 at the power end.
A rotary ball type microorganism two-stage expanding culture method comprises the following steps:
1. closing the manhole 05, placing the electromagnetic directional valve 13 in the pipeline 16 passage, and closing the sampling pipe 15, the pipeline pump valve 19, the additive valve 20 and the discharge valve 26;
2. and (3) opening the feed valve 09, and pumping the strain culture expanding mixed liquid into the hollow ball 06 from the material inlet and outlet 25 of the cross pipe along the gap between the inner wall of the cross pipe 10 and the outer wall of the vacuum jet pump 12.
3. The amount of the added strain culture liquid mixture is controlled at the upper part of the hollow shaft 03 at the power end (the volume of the water-gas mixture in the hollow ball 06 is increased due to the oxygen brought by the negative pressure when the vacuum jet pump 12 is started), and the liquid level of the water-gas mixture is not larger than the excess air inlet of the exhaust port 04 in principle.
4. Feed valve 09 is closed and magnetic line pump valve 19 is opened.
5. The magnetic circulating pump 18 is started to make the strain mixed liquid in the working chamber pass through the vacuum jet pump 12 and the cross pipe material inlet and outlet 25 to be sprayed into the working chamber together with oxygen, and then the transmission mechanism is started to make the whole hollow ball enter a rotation mode and enter a normal operation state (the specific speed is set according to different microbial strains).
6. Different propagation time is selected according to different microbial strains, after a detection sample (sampled and detected by a sampling pipe 15) is qualified, the electromagnetic directional valve 13 is placed in a discharge state by cutting off a passage of the vacuum ejector 12, so that the material is lifted out of the discharge port 14. If the machine is stopped for a long time or different culture expanding strains are replaced, the discharge valve 26 can be opened to completely discharge the culture.
Although the present invention has been described with reference to a preferred embodiment, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (3)
1. A rotary ball type microorganism two-stage expanding culture device comprises a rotary ball type expanding culture chamber, a power transmission mechanism and a feeding injection oxygen supply mechanism;
the cultivation method is characterized in that the spin-ball type cultivation expanding chamber comprises a hollow ball, a manhole is arranged above the hollow ball, a power end hollow shaft, a power end outer bearing and a power end inner bearing are arranged at the left end of the hollow ball, the power end inner bearing is arranged inside the power end outer bearing, and the power end hollow shaft is fixed in the middle of the power end inner bearing; the right end is provided with a spraying end hollow shaft, a spraying end outer sealing gland and a spraying end inner bearing, the spraying end inner bearing is arranged inside the spraying end outer bearing, and the spraying end hollow shaft is fixed in the middle of the spraying end inner bearing; the bottom of the rotary ball type expanding culture chamber is provided with a discharge valve, the periphery of the discharge valve is also provided with a plurality of lifting stirring barrels, and the rotary ball type expanding culture chamber is also provided with an exhaust pipe communicated with the inside and the outside;
the power transmission mechanism comprises a large transmission turbine, a power worm and a power end base, wherein the power end base is of an L-shaped structure, the power worm is arranged above the transverse plane of the bottom of the power end base, the axis of the large transmission turbine is arranged on an outer power end bearing, and the bottom of the outer power end bearing is arranged on the power end base;
the feeding and spraying oxygen supply mechanism comprises a pipeline, a cross pipe, a feeding valve, a vacuum injection pump, an electromagnetic directional valve, a constant-temperature electromagnetic heater, a magnetic circulating pump, an additive valve, an additive batching tank, a discharge port and a sampling pipe; the pipeline comprises a straight pipeline section 1, an arc section 1 and a broken pipeline section 1, wherein one end of the broken pipeline section is directly connected with one end of the arc section, the other end of the broken pipeline section is connected with the other end of the arc section through a cross pipe, the left end of the cross pipe penetrates through a hollow shaft at a spraying end and enters the hollow sphere, the tail end of the pipeline positioned at the inner side of the hollow sphere is used as an ejector outlet, the ejector outlet is inclined downwards, a vacuum jet pump and an electromagnetic reversing valve are sequentially arranged on the right side of the cross pipe, the bottom of the electromagnetic reversing valve is a discharge outlet, a downward sampling pipe is arranged on the pipeline on the right side of the electromagnetic reversing valve, an additive batching tank, a magnetic circulating pump and a constant temperature electromagnetic heater are sequentially arranged on the broken pipeline section from top to bottom, the additive batching tank is connected to the broken pipeline section, the additive batching tank is provided with an additive valve, and the magnetic circulating pump is provided with a pipeline pump valve;
one end of the exhaust pipe penetrates through the hollow shaft at the power end and extends into the hollow ball, the exhaust pipe in the hollow ball is bent upwards after entering the hollow ball and reaches the position near a population, the bent shape of the exhaust pipe is matched with the arc shape of the inner wall of the air ball, the other end of the exhaust pipe extends out of the hollow shaft at the power end for a section and then is erected upwards, a fixed stand column is arranged, the upper end of the fixed stand column is connected to the exhaust pipe, and the lower end of the fixed stand column is fixed on the base at the power end.
2. A rotary ball type microorganism secondary amplification culture method, which adopts the rotary ball type microorganism secondary amplification culture device as claimed in claim 1, and is characterized by comprising the following steps:
s1, closing a manhole, placing an electromagnetic directional valve in a pipeline passage, and closing a sampling pipe, a pipeline pump valve, an additive valve and a discharge valve;
s2, opening a feed valve, pumping in a strain culture expanding mixed solution, and enabling the mixed solution to enter the hollow ball from the material inlet and the material outlet of the cross pipe along the gap between the inner wall of the cross pipe and the outer wall of the vacuum jet pump;
s3, controlling the adding amount of the strain culture expanding mixed liquid at the upper part of the hollow shaft at the power end, wherein the liquid level of the water-gas mixed liquid must not exceed the excess air inlet of the exhaust port in principle;
s4, closing the feeding valve and opening the magnetic pipeline pump valve;
s5, starting the magnetic circulating pump to make the strain mixed liquid in the working chamber sprayed into the working chamber with oxygen through the vacuum jet pump and the cross pipe material inlet and outlet 25, and then starting the transmission mechanism to make the whole hollow ball enter a rotation mode and enter a normal operation state;
and S6, selecting different propagation time according to different microbial strains, cutting off a passage of the vacuum ejector after the detected sample is qualified, and placing the electromagnetic directional valve in a discharging state to enable the material to be lifted out from a discharging port.
3. The two-stage propagation method of rolling ball type microorganism as claimed in claim 2, further comprising step S7, if it is a long-term shutdown or different propagation strains are changed, the discharging valve is opened to discharge the microorganism completely.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811554121.5A CN109439515B (en) | 2018-12-19 | 2018-12-19 | Rotary ball type microorganism two-stage expanding culture device and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811554121.5A CN109439515B (en) | 2018-12-19 | 2018-12-19 | Rotary ball type microorganism two-stage expanding culture device and method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109439515A CN109439515A (en) | 2019-03-08 |
| CN109439515B true CN109439515B (en) | 2022-03-08 |
Family
ID=65559390
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811554121.5A Active CN109439515B (en) | 2018-12-19 | 2018-12-19 | Rotary ball type microorganism two-stage expanding culture device and method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109439515B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2725092A1 (en) * | 2012-10-23 | 2014-04-30 | Instytut Agrofizyki im. Bohdana Dobrzanskiego PAN | Device for breeding phototropic micro-organisms |
| CN206814753U (en) * | 2017-06-06 | 2017-12-29 | 南京追梦生物科技有限公司 | It is a kind of to rotate and with the micro algae culturing device of light reflection |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005113741A1 (en) * | 2004-05-12 | 2005-12-01 | Board Of Regents Of University Of Nebraska | Vortex tube thermocycler |
| CN201241154Y (en) * | 2008-06-20 | 2009-05-20 | 重庆大学 | White-rot fungi solid fermentation system |
| CN201737943U (en) * | 2009-12-30 | 2011-02-09 | 广东海洋大学 | Rotary barrel type solid-state fermentation equipment |
| CN102181364B (en) * | 2011-03-11 | 2013-01-30 | 北京航空航天大学 | Device for culturing in vitro cells through single axis rotating and online shearing |
| GB2507109A (en) * | 2012-10-19 | 2014-04-23 | Advanced Technology And Engineering Ltd Atel | Fermenter comprising gas and liquid re-circulation loops |
| NL2016597B1 (en) * | 2016-04-13 | 2017-11-07 | Minibrew Holding B V | Minibrewery. |
| JP2020014385A (en) * | 2016-11-14 | 2020-01-30 | 株式会社フラウス | Algae culture apparatus |
| CN206927882U (en) * | 2017-07-20 | 2018-01-26 | 潍坊泽成生物技术有限公司 | A kind of efficient biomass installation for fermenting for being uniformly distributed material |
| CN207877715U (en) * | 2018-01-15 | 2018-09-18 | 山东省食品发酵工业研究设计院 | A kind of injecting type gas-liquid cycle fermentation tank |
| CN108690800B (en) * | 2018-06-28 | 2023-11-21 | 湖北希普生物科技有限公司 | Solid fermentation tank |
| CN108795704B (en) * | 2018-09-06 | 2023-09-29 | 辽阳博仕流体设备有限公司 | Oxygen supply fermentation system |
-
2018
- 2018-12-19 CN CN201811554121.5A patent/CN109439515B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2725092A1 (en) * | 2012-10-23 | 2014-04-30 | Instytut Agrofizyki im. Bohdana Dobrzanskiego PAN | Device for breeding phototropic micro-organisms |
| CN206814753U (en) * | 2017-06-06 | 2017-12-29 | 南京追梦生物科技有限公司 | It is a kind of to rotate and with the micro algae culturing device of light reflection |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109439515A (en) | 2019-03-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102220223B (en) | Solid-state fermentation material processing device | |
| CN104277968A (en) | Large-scale biogas hydraulic-circulating even mixing device | |
| CN205223254U (en) | Bio -pharmaceuticals fermentation cylinder | |
| CN104403942A (en) | Solid material fermentation device | |
| CN110656043A (en) | Microbial fermentation tank for producing liquid feed additive | |
| CN102628016A (en) | Totally enclosed water-gas linkage intermittent internal rotation anaerobic digestion device with constant temperature for house refuse | |
| CN110656044A (en) | Novel intelligent microbial fermentation device | |
| CN210394377U (en) | Anaerobe culture system | |
| CN205042861U (en) | Bionics biotechnology handles organic solid waste's intelligent device | |
| CN109439515B (en) | Rotary ball type microorganism two-stage expanding culture device and method | |
| CN102887735B (en) | Fermentation reactor | |
| CN108441416A (en) | A kind of static fermentation device and its fermentation process and application | |
| CN204311063U (en) | Solid substances fermentation equipment | |
| CN208414429U (en) | A kind of static fermentation device | |
| CN206502818U (en) | A kind of microbial cultivation device | |
| CN203855575U (en) | Culture fermentation tank for lucid ganoderma strain | |
| CN201384050Y (en) | Mixing conveyor for wet-mixing fodder | |
| CN201506790U (en) | Fermentation tank | |
| CN207775218U (en) | A kind of full automatic microorganism installation for fermenting | |
| CN204509251U (en) | A kind of energy-saving agent production line | |
| CN104195043B (en) | A kind of can the horizontal rotation type bio-reactor of sterilizing online | |
| CN107022471B (en) | Microbial fermentation system and fermentation method for organic planting | |
| CN206760739U (en) | A kind of Feed Manufacturing activates device | |
| CN204727864U (en) | A kind of fermentation unit for alcoholic | |
| CN209872937U (en) | Liquid fermentation culture bacillus device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 101, No. 321, North Section of Shixin Road, Nancun Town, Panyu District, Guangzhou City, Guangdong Province, 510000 Patentee after: Guangdong Jiabao Conveying Machinery Co.,Ltd. Address before: No. 953, Shixin Road, luobian village, Nancun Town, Panyu District, Guangzhou, Guangdong 510000 Patentee before: GUANGZHOU JIABAO MACHINERY Co.,Ltd. |
|
| CP03 | Change of name, title or address |