CN209636251U - A kind of tumour cell 3D culture apparatus - Google Patents
A kind of tumour cell 3D culture apparatus Download PDFInfo
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- CN209636251U CN209636251U CN201920185796.0U CN201920185796U CN209636251U CN 209636251 U CN209636251 U CN 209636251U CN 201920185796 U CN201920185796 U CN 201920185796U CN 209636251 U CN209636251 U CN 209636251U
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- storage bin
- culture
- rocker
- cell
- tumour cell
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Abstract
The utility model belongs to cell culture apparatus technical field, in particular a kind of tumour cell 3D culture apparatus, including storage bin, temperature and humidity controller and storage bin upper surface have been fixed by screws on one side surface of storage bin integrally formed with snorkel, the storage bin is internally provided with rocker, spring is provided between the two sides of the rocker and the inner wall of the storage bin, the utility model is during use, pass through the components such as air pump in storage bin and spring, the cell of the culture on culture dish in culture hole may make uniformly to be distributed, it is taken considerable time to solve the problems, such as that original needs artificially shake up operation to tumour cell progress 3D culture, utilize the fixed plate and net leakage in rocker, the liquid waste of failure cell and its existence that experiment culture can be enabled to generate flows into the recycling box of lower section, so that thin Born of the same parents' waste liquid is quickly handled, and is increased the working efficiency of experiment and is improved the practicability of device.
Description
Technical field
The utility model belongs to cell culture apparatus technical field, and in particular to a kind of tumour cell 3D culture apparatus.
Background technique
Cell clone technology is also in cell culture, and the regular noun in biology is cell culture technology, no matter for
For entire biotechnology, or one of them biological cloning technology, cell culture is all an essential mistake
Journey, the extensive clone of cell culture inherently cell, cell culture technology can by a cell by mass propgation at
For simple many cells that are unicellular or seldom breaking up, this is the essential link of clone technology, and cell culture itself
It is exactly the clone of cell, a large amount of cell or its metabolite is obtained by cell culture, because biological product is all from cell
It gets, it is possible to say that cell culture technology is most crucial, most basic technology in biotechnology.
With the continuous development of modern science and technology, 3D cell culture technology is also constantly improving, and 3D cell culture is can be
The culture technique for being more nearly the microenvironment of internal life condition is provided for cell in cell cultivation process, but now
Cell culture apparatus is difficult to comprehensively cultivate cell culture, during use as just the storage for placing cell
Device enables original anti-tumor experiment tumor cell culture device have certain limitation during use, and right
The course of work that tumour cell shake up during 3D culture culture dish is relatively complicated, takes a substantial amount of time, and nothing
Method quickly and effectively disposes the failure cell that experiment culture generates and the liquid waste of its existence, reduces experimental work effect
Rate.
Utility model content
To solve the problems mentioned above in the background art.The utility model provides a kind of tumour cell 3D culture dress
It sets, has the characteristics that there are more practicabilities.
To achieve the above object, the utility model provides the following technical solutions: a kind of tumour cell 3D culture apparatus, including
Storage bin, storage bin a side surface on be fixed by screws temperature and humidity controller and storage bin upper surface one at
Type has snorkel, and the storage bin is internally provided with rocker, sets between the two sides of the rocker and the inner wall of the storage bin
It is equipped with spring, one end of the spring is bumped into the storage bin, and the other end is bumped into the rocker, the inside card of the rocker
Conjunction has fixed plate, and the upper surface of the fixed plate is connected together and has culture dish, if the upper surface of the culture dish, which is equally spaced, to be had
Dry culture hole, the two sides of the culture dish are provided with limit plate, and the limit plate is an integral structure with the fixed plate, institute
The side for stating rocker is provided with air pump, and the air pump is bolted to connection on the inner wall of the storage bin, the air pump
Output end be rotatably connected to telescopic rod, the telescopic rod interts different from one end of the air pump into the inside of the rocker, institute
Stating the side engaging in rocker different from the fixed plate has net to leak.
Preferably, motor, the output end of the motor have been bolted to connection on the top surface inside the storage bin
It is rotatably connected to bull stick, bull stick back is provided with turntable in one end of the motor, and bull stick wears the inside for tapping into the turntable, turntable
Surface on offer circular hole.
Preferably, it is rotatably connected to chassis on the bottom surface inside the storage bin, being fixed above chassis has recycling
The inside of box, recycling box is placed with bleaching powder.
Preferably, the side of the storage bin integrally formed with linking platform, be connected in the front surface of platform open up it is fluted, it is recessed
Weighing scale has been fixed by screws on bottom surface inside slot.
Preferably, the front side of the storage bin is hinged with lid door by hinge, and the middle position on lid door surface is chimeric to be had
Observation window.
Preferably, it is welded with pedestal at the corner location of the storage bin lower surface, the outside of pedestal is socketed with Anti-slip cover.
Preferably, fixture block is bonded with by glue on the two sides inner wall of the storage bin.
Compared with prior art, the utility model has the beneficial effects that the utility model during use, passes through storage
The components such as air pump and the spring in case are deposited, may make the cell of the culture on culture dish in culture hole to be uniformly distributed, to solve
Original needs of having determined artificially carry out 3D culture to tumour cell and shake up the problem of operation takes considerable time, utilize consolidating in rocker
Fixed board and net leak, and the liquid waste of failure cell and its existence that experiment culture can be enabled to generate flows into the recycling box of lower section,
So that cell waste liquid is quickly handled, increases the working efficiency of experiment and improve the practicability of device.
Detailed description of the invention
Attached drawing is used to provide a further understanding of the present invention, and constitutes part of specification, practical with this
Novel embodiment is used to explain the utility model together, does not constitute limitations of the present invention.In the accompanying drawings:
Fig. 1 is the structural schematic diagram of the utility model；
Fig. 2 is structural schematic diagram external in the utility model；
Fig. 3 is the structural schematic diagram of rocker top surface in the utility model；
Fig. 4 is the structural schematic diagram amplified at A in Fig. 1；
Fig. 5 is the structural schematic diagram of culture dish in the utility model.
In figure: 1, storage bin；2, it is connected platform；3, rocker；4, spring；5, culture dish；6, chassis；7, recycling box；8, motor；
9, turntable；10, fixture block；11, lid door；12, groove；13, weighing scale；14, observation window；15, temperature and humidity controller；16, air pump；
17, telescopic rod；18, limit plate；19, fixed plate；20, net leakage；21, culture hole；22, snorkel.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
Embodiment 1
Fig. 1-5 is please referred to, the utility model is the following technical schemes are provided: a kind of tumour cell 3D culture apparatus, including storage
It deposits and has been fixed by screws 1 upper surface one of temperature and humidity controller 15 and storage bin on a side surface of case 1, storage bin 1
It is body formed to have snorkel 22, it can be passed through carbon dioxide into storage bin 1 by snorkel 22, in the present embodiment, Temperature and Humidity Control
The model HTC-201 of device 15, by the way that the temperature variations in storage bin 1 can be clearly seen on temperature and humidity controller 15,
And be timely adjusted and control, it is normally grown with the cell inside guaranteeing, storage bin 1 is internally provided with rocker
3, spring 4 is provided between the two sides of rocker 3 and the inner wall of storage bin 1, one end of spring 4 is bumped into storage bin 1, other end edge
Enter in rocker 3, the inside engaging of rocker 3 has fixed plate 19, and the upper surface of fixed plate 19, which is connected together, culture dish 5, culture dish 5
Upper surface be equally spaced and have several culture holes 21, the two sides of culture dish 5 are provided with limit plate 18, limit plate 18 and fixed plate
19 are an integral structure, and the side of rocker 3 is provided with air pump 16, in the present embodiment, the model TWC-180810 of air pump 16, and gas
Pump 16 is bolted to connection on the inner wall of storage bin 1, and the output end of air pump 16 is rotatably connected to telescopic rod 17, this implementation
In example, the model FT01 of telescopic rod 17, telescopic rod 17 interts different from one end of air pump 16 into the inside of rocker 3, different in rocker 3
In the side of fixed plate 19, engaging has net leakage 20, by the air pump 16 and the equal components of spring 4 in storage bin 1, may make culture dish 5
The cell of culture in upper culture hole 21 is uniformly distributed.
Specifically, being bolted to connection motor 8 on top surface inside storage bin 1, in the present embodiment, motor 8 is
Dongguan City Morse automates the motor of the model SST86D1605 of Science and Technology Ltd.'s production, the output end rotation of motor 8
It is connected with bull stick, bull stick back is provided with turntable 9 in one end of motor 8, and bull stick wears the inside for tapping into turntable 9, on the surface of turntable 9
Circular hole is offered, centrifuge tube is fastened on turntable 9, starts motor 8, drives the centrifuge tube on turntable 9 to be revolved by bull stick
Turn, so that internal cell is quickly centrifugated, increases the practicability of anti-tumor experiment tumor cell culture device.
Specifically, being rotatably connected to chassis 6 on bottom surface inside storage bin 1, the top of chassis 6 is fixed and has recycling box
7, the inside of recycling box 7 is placed with bleaching powder, after cell waste liquid is fallen in the recycling box 7 on chassis 6 by net leakage 20, rotation
Recycling box 7 neutralizes the bleaching powder of internal storage and cell waste liquid, then quickly disposes the liquid after neutralization, prevents
Waste liquid directly outwells the case where generation is directly polluted.
Specifically, the side of storage bin 1 integrally formed with linking platform 2, is connected in the front surface of platform 2 and opens up fluted 12,
It has been fixed by screws weighing scale 13 on bottom surface inside groove 12, in the present embodiment, the model QX- of weighing scale 13
B37 can snap-fit into centrifuge tube in groove 12 on weighing scale 13 among integrally formed square block, using weighing scale 13 can to from
Cell liquid in heart pipe carries out the measurement of content.
Specifically, the front side of storage bin 1 is hinged with lid door 11 by hinge, the middle position on 11 surface of lid door is chimeric to be had
Observation window 14, that inside when having any situation, can be can be observed inside storage bin 1 by the observation window 14 in lid door 11
Situation.
Specifically, being welded with pedestal at the corner location of 1 lower surface of storage bin, the outside of pedestal is socketed with Anti-slip cover, prevents
Sliding sleeve can prevent when to sequence of operations is carried out inside storage bin 1, and storage bin 1 will not be moved easily, guarantee work just
Often carry out.
Specifically, fixture block 10 is bonded with by glue on the two sides inner wall of storage bin 1, in the process for carrying out cell observation
In, centrifuge tube can be clamped in fixture block 10, and labelled convenient for observing and identifying.
The working principle and process for using of the utility model: the utility model during use, cryopreservation tube from liquid
It takes out, puts into 37 DEG C of water-baths immediately, gentle agitation in nitrogen, above-mentioned cell suspension is drawn onto centrifuge tube after all melting by liquid
In, centrifuge tube is fastened on turntable 9, motor 8 is started, drives the centrifuge tube on turntable 9 to be rotated by bull stick, so that interior
The cell in portion is quickly centrifugated, then by the cell liquid of centrifuge tube pour into the culture hole 21 in 3 top culture dish 5 of rocker it
In, culture dish 5 will not be deviated easily under the action of limit plate 18, then booster air pump 16, in telescopic rod 17 and spring 4
It, will be in rocker 3 after experiment so as to effectively carry out passage assays so that cell is uniformly distributed in cell liquid under driving
Fixed plate 19 is pulled to side, so that the cell waste liquid after culture is fallen in the recycling box 7 on chassis 6 by net leakage 20, together
When rotation recycling box 7 make internal storage bleaching powder and cell waste liquid neutralize after, the liquid after neutralization is quickly disposed,
During carrying out cell observation, centrifuge tube can be clamped in fixture block 10, and labelled convenient for identification, and by centrifuge tube
The upper of weighing scale 13 in groove 12 is snap-fited into, the measurement of content, In can be carried out to the cell liquid in centrifuge tube using weighing scale 13
The temperature variations in storage bin 1 can be clearly seen on temperature and humidity controller 15, and are timely controlled, and lid door is passed through
The situation inside storage bin 1 can be observed in observation window 14 on 11, is timely adjusted.
Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention, it is not limited to this
Utility model, although the utility model is described in detail with reference to the foregoing embodiments, for those skilled in the art
For, it is still possible to modify the technical solutions described in the foregoing embodiments, or to part of technical characteristic
It is equivalently replaced.Within the spirit and principle of the utility model, any modification, equivalent replacement, improvement and so on,
It should be included within the scope of protection of this utility model.
Claims (7)
1. a kind of tumour cell 3D culture apparatus, including storage bin (1), storage bin (1) a side surface on be screwed
Temperature and humidity controller (15) and storage bin (1) upper surface are connected with integrally formed with snorkel (22), it is characterised in that: described
Storage bin (1) is internally provided with rocker (3), is provided between the two sides of the rocker (3) and the inner wall of the storage bin (1)
Spring (4), one end of the spring (4) are bumped into the storage bin (1), and the other end is bumped into the rocker (3), the rocker
(3) inside engaging has fixed plate (19), and the upper surface of the fixed plate (19) is connected together and has culture dish (5), the culture
The upper surface of ware (5), which is equally spaced, several culture holes (21), and the two sides of the culture dish (5) are provided with limit plate (18),
The limit plate (18) is an integral structure with the fixed plate (19), and the side of the rocker (3) is provided with air pump (16), institute
It states air pump (16) to be bolted to connection on the inner wall of the storage bin (1), the output end of the air pump (16) rotates company
It is connected to telescopic rod (17), the telescopic rod (17) interts the inside into the rocker (3) different from one end of the air pump (16), institute
Stating the side engaging in rocker (3) different from the fixed plate (19) has net leakage (20).
2. a kind of tumour cell 3D culture apparatus according to claim 1, it is characterised in that: the storage bin (1) is internal
Top surface on be bolted to connection motor (8), the output end of the motor (8) is rotatably connected to bull stick, bull stick back in
One end of the motor (8) is provided with turntable (9), and bull stick wears the inside for tapping into the turntable (9), is opened on the surface of turntable (9)
Equipped with circular hole.
3. a kind of tumour cell 3D culture apparatus according to claim 1, it is characterised in that: the storage bin (1) is internal
Bottom surface on be rotatably connected to chassis (6), being fixed above chassis (6) has recycling box (7), and the inside of recycling box (7) is put
It is equipped with bleaching powder.
4. a kind of tumour cell 3D culture apparatus according to claim 1, it is characterised in that: the one of the storage bin (1)
Side is connected in the front surface of platform (2) and opens up fluted (12) integrally formed with linking platform (2), on the internal bottom surface of groove (12)
It has been fixed by screws weighing scale (13).
5. a kind of tumour cell 3D culture apparatus according to claim 1, it is characterised in that: before the storage bin (1)
Side is hinged with lid door (11) by hinge, and the middle position on lid door (11) surface is chimeric observation window (14).
6. a kind of tumour cell 3D culture apparatus according to claim 1, it is characterised in that: storage bin (1) following table
Pedestal is welded at the corner location in face, the outside of pedestal is socketed with Anti-slip cover.
7. a kind of tumour cell 3D culture apparatus according to claim 1, it is characterised in that: the two of the storage bin (1)
Fixture block (10) are bonded with by glue on the inner wall of side.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920185796.0U CN209636251U (en) | 2019-01-30 | 2019-01-30 | A kind of tumour cell 3D culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920185796.0U CN209636251U (en) | 2019-01-30 | 2019-01-30 | A kind of tumour cell 3D culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209636251U true CN209636251U (en) | 2019-11-15 |
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ID=68493081
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920185796.0U Expired - Fee Related CN209636251U (en) | 2019-01-30 | 2019-01-30 | A kind of tumour cell 3D culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209636251U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114410451A (en) * | 2022-01-17 | 2022-04-29 | 罗志勇 | Tumor cell culture device for oncology department |
-
2019
- 2019-01-30 CN CN201920185796.0U patent/CN209636251U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114410451A (en) * | 2022-01-17 | 2022-04-29 | 罗志勇 | Tumor cell culture device for oncology department |
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|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191115 |