CN208517432U - A kind of auxiliary device of suspension cell culture - Google Patents
A kind of auxiliary device of suspension cell culture Download PDFInfo
- Publication number
- CN208517432U CN208517432U CN201820967686.5U CN201820967686U CN208517432U CN 208517432 U CN208517432 U CN 208517432U CN 201820967686 U CN201820967686 U CN 201820967686U CN 208517432 U CN208517432 U CN 208517432U
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- China
- Prior art keywords
- accessory plate
- cell culture
- suspension cell
- auxiliary device
- graduation mark
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Abstract
The utility model discloses a kind of auxiliary device of suspension cell culture: including accessory plate, wedge-shaped transition plates, rear baffle and hand-held seat, the accessory plate is the mellow and full rectangular slab in corner, with a thickness of 0.5-0.8cm, the evenly spaced peep hole of at least six is opened up on accessory plate, wedge-shaped transition plates is installed on the right side of accessory plate short side, left side is installed by rear baffle, hand-held seat is installed in side middle position before and after accessory plate, the auxiliary plate surface is equipped with lateral graduation mark and longitudinal graduation mark, cell culture bags are gently moved to accessory plate, and tiling is on it, the observation of microscope observation platform can be steadily placed in, avoid the shaking of culture solution and mixed influence result in movement and observation, facilitate look at the activity of suspension cell, proliferation trend；Its structure is simple, convenient and practical, cheap, effectively improves culture efficiency.
Description
Technical field
The utility model relates to immunocyte culture technique fields, are specifically related to a kind of auxiliary dress of suspension cell culture
It sets.
Background technique
Tumor biotherapy industry needs to turn out a large amount of immunocyte, usually makes when carrying out immune cell therapy
It is carried out with Tissue Culture Flask, when carrying out cell culture using culture bottle, since culture bottle ventilation volume is small, cell Proliferation is caused to reach
Less than desired effect: and then cell culture bags are had devised, solve the problems, such as that ventilation volume is small and cell proliferation rate is slow, together
When suspension cell culture bag use, cell culture bags be closed culture, reduce cell contamination and operator infection machine
Rate, gas exchange area is big, is conducive in limited culture space mass propgation.
But cell culture bags are in use, there is also following defects: firstly, cell culture bags use EVA material
Whether preparation, in order to observe cell Proliferation trend and pollute, needs in entire cell cultivation process for transparent flexible material
It moves culture bag and culture bag is placed on microscope observation platform, having 1000 milliliters or more in culture solution is because of micro- sem observation
Platform size cannot completely area match culture bag size, cause liquid sloshing and mixing, be unfavorable for observe cell activity and proliferation become
Whether gesture, the muddiness for being less susceptible to resolution fluid cause because of pollution；Secondly, the prior art is disclosed for moving cell culture bags
Pallet (such as 206418107 U of CN) be directly to take out culture bag to be placed on support during mobile culture bag to pallet
On disk, culture bag cannot be gently moved to pallet, practice effect is poor, and pallet itself has one i.e. in moving process
Determine thickness, can cause to generate vibration in moving process due to thickness in slowly dragging culture bag；Finally, cell culture
Bag also can not tentatively distinguish the preliminary area of the cell of culture in use, it has not been convenient to use.
Utility model content
In view of the above-mentioned problems existing in the prior art, the utility model provides a kind of auxiliary dress of suspension cell culture
It sets, cell culture bags is gently moved to accessory plate, and tile on it, can steadily be placed in the observation of microscope observation platform, keep away
Exempt from the shaking of culture solution and mixed influence in movement and observation as a result, facilitating look at the activity of suspension cell, is proliferated trend；
Its structure is simple, convenient and practical, cheap, effectively improves culture efficiency.
To realize above-mentioned technical purpose and the technique effect, the utility model is achieved through the following technical solutions:
A kind of auxiliary device of suspension cell culture, including accessory plate, wedge-shaped transition plates, rear baffle and hand-held seat, it is described
Accessory plate is that the mellow and full rectangular slab in corner opens up the evenly spaced observation of at least six with a thickness of 0.5-0.8cm on accessory plate
Wedge-shaped transition plates is installed on hole, accessory plate short side right side, and rear baffle is installed in left side, and side middle position is installed hand-held before and after accessory plate
Seat, the auxiliary plate surface are equipped with lateral graduation mark and longitudinal graduation mark.
Preferably, the accessory plate is clear perspex material, and the length and width of accessory plate is identical as cell culture bags.
Preferably, the wedge-shaped transition board rear end is fixedly connected with accessory plate, and the thickness of rear end is identical as accessory plate, front end
With a thickness of 0.1cm；
The wedge shape transition plates front end corner transition circle profit.
Preferably, the diameter of the peep hole can be identical or not identical.
Preferably, there is lateral graduation mark in auxiliary plate surface transverse center position, and both ends are equipped with longitudinal graduation mark, and can
Equipped with scale marker.
Preferably, the hand-held seat is the plate that two turned-out angles are 90 °.
The operating method of the utility model are as follows: first that accessory plate band wedge shape transition plates is close when preparing to shift culture bag
Cell culture bags slowly move cell culture bags from one end, until entire cell culture bags are laid on accessory plate (i.e. cell
Culture bag rear end reaches rear baffle), while gauffer is checked whether there is, culture solution is not shaken as far as possible in the whole process in order to avoid influencing
Subsequent observation；Hold seat by hand-held stop when mobile and move together accessory plate together with culture bag, it is mobile in keep balance, later
It is placed on microscope observation platform；Peep hole is placed on object lens by adjustment position, passes through the thin of tapping observation culture
Born of the same parents；It simultaneously can be according to lateral graduation mark and longitudinal graduation mark preresearch estimates cell gross area.
The utility model has the beneficial effects that avoiding moving since cell culture bags are mainly used for suspension cell culture
Avoid the muddiness that culture solution is caused after the shaking and mixing of liquid when dynamic and observation and the liquid that is not easily distinguishable with the presence or absence of pollution, this
The wedge-shaped transition plates being arranged on utility model accessory plate avoids caused shaking during being moved to accessory plate；Accessory plate and
The rear baffle of rear end setting, can make culture bag not generate shaking in moving process, can also be laid in microscope observation platform；It is auxiliary
The peep hole that plate opens up is helped, can be observed at different peep holes by mobile to observe the cell culture of entire culture bag
Journey can also observe cell through auxiliary frame, according to lateral graduation mark and longitudinal graduation mark because being made of organic glass
The preresearch estimates cell gross area；The use of accessory plate is that mobile culture bag is more convenient, not will cause its breakage, will not cause to train
The mixing of nutrient solution facilitates look at the activity of suspension cell, is proliferated trend, differentiates culture solution with the presence or absence of pollution, to cultivate
Journey provides important guarantee, and structure is simple, convenient and practical, cheap, effectively improves culture efficiency.
Certainly, any product for implementing the utility model does not necessarily require achieving all the advantages described above at the same time.
Detailed description of the invention
In order to illustrate more clearly of the technical solution of the utility model embodiment, make required for being described below to embodiment
Attached drawing is briefly described, it should be apparent that, the drawings in the following description are merely some embodiments of the present invention,
For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings
Other attached drawings.
Fig. 1 is the structural schematic diagram of the auxiliary device of suspension cell culture described in the utility model embodiment；
Fig. 2 is the structural schematic diagram of accessory plate described in the utility model embodiment；
Fig. 3 is the use schematic diagram for the auxiliary device that the utility model implements the suspension cell culture；
In attached drawing, parts list represented by the reference numerals are as follows:
1- accessory plate, 2- peep hole, 3- wedge shape transition plates, 4- rear baffle, 5- hold seat, the longitudinal direction 6- graduation mark, 7- transverse direction
Graduation mark.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without creative efforts
All other embodiment obtained, fall within the protection scope of the utility model.
Embodiment
As shown in Figure 1-3, a kind of auxiliary device of suspension cell culture, including accessory plate 1, wedge-shaped transition plates 3, rear baffle
4 and hand-held seat 5, the accessory plate 1 is that the mellow and full rectangular slab in corner opens up at least 6 with a thickness of 0.5-0.8cm on accessory plate 1
A evenly spaced peep hole 2, installs wedge-shaped transition plates 3 on the right side of accessory plate 1 short side, and rear baffle 4 is installed in left side, before accessory plate 1
Hand-held seat 5 is installed in rear side middle position, and 1 surface of accessory plate is equipped with lateral graduation mark 7 and longitudinal graduation mark 6；
The accessory plate 1 is clear perspex material, and the length and width of accessory plate 1 is identical as cell culture bags；
3 rear end of the wedge shape transition plates is fixedly connected with accessory plate 1, and the thickness of rear end is identical as accessory plate 1, nose thickness
For 0.1cm；
Wedge shape 3 front end corner transition circle of the transition plates profit；
The diameter of the peep hole 2 can be identical or not identical；
There is lateral graduation mark 7 in the 1 surface transverse center position of accessory plate, and both ends are equipped with longitudinal graduation mark 6, and can be equipped with
Scale marker；
The hand-held seat 5 is the plate that two turned-out angles are 90 °.
Specific embodiment: it in the cell of cell culture bags culture, needs to prepare to shift culture bag when being detected, first will
Accessory plate 1, close to cell culture bags, cell culture bags is slowly moved from one end with wedge-shaped transition plates 3, until entire cell is trained
Feeding bag is laid on accessory plate 1 (i.e. cell culture bags rear end reach in rear baffle 4) during this, does not need cell culture directly
Bag pick up, cell culture bags during being placed on accessory plate 1 because there is the presence of wedge-shaped transition plates 3, will not be because of accessory plate 1
Thickness causes to cause to vibrate in moving process, and then causes its culture solution to shake, while checking whether there is gauffer, in entire mistake
Culture solution is not shaken as far as possible in journey in order to avoid influencing subsequent observation, since cell culture bags are mainly used for suspension cell culture, because
This is avoided the shaking that liquid is avoided in movement and observation and the muddiness that culture solution is caused after mixing and whether the liquid that is not easily distinguishable
There are pollutions；Accessory plate 1 and the rear baffle 4 of rear end setting, can make culture bag not generate shaking in moving process；When mobile
Hold seat 5 by hand-held stop and move together accessory plate 1 together with culture bag, it is mobile in keep balance, be placed on later micro-
Sem observation platform, accessory plate 1 are laid in microscope observation platform, and peep hole 2 is placed on object lens by adjustment position, pass through peep hole 2
The cell of place's observation culture；It will not excessively cause microscope observation platform that cannot place because of culture solution and cause mixing etc., by auxiliary
Cell culture bags are steadily placed and are observed by plate 1, and the use of accessory plate 1 is that mobile culture bag is more convenient, and it is broken not will cause it
Damage, will not cause the mixing of culture solution, facilitate look at the activity of suspension cell, be proliferated trend, and differentiating culture solution whether there is
Pollution, important guarantee is provided for incubation；Accessory plate 1 can also be seen because being made of organic glass through accessory plate 1
Cell is observed, can be passed through that is, when observing cell according to lateral graduation mark 7 and longitudinal 6 preresearch estimates cell gross area of graduation mark
The first approximation of data for reading lateral graduation mark 7 and longitudinal graduation mark 6 calculates the general area of cell, to hold cultivation cycle etc.,
Its structure is simple, convenient and practical, cheap, effectively improves culture efficiency.
In the description of this specification, the description of reference term " one embodiment ", " example ", " specific example " etc. means
Particular features, structures, materials, or characteristics described in conjunction with this embodiment or example are contained at least one of the utility model
In embodiment or example.In the present specification, schematic expression of the above terms be not necessarily referring to identical embodiment or
Example.Moreover, particular features, structures, materials, or characteristics described can be in any one or more embodiment or examples
In can be combined in any suitable manner.
The preferred embodiment in the utility model disclosed above is only intended to help to illustrate the utility model.Preferred embodiment is simultaneously
There is no the details that detailed descriptionthe is all, also not limiting the utility model is only the specific embodiment.Obviously, according to this theory
The content of bright book can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is in order to preferably
The principles of the present invention and practical application are explained, so that skilled artisan be enable to better understand and utilize this
Utility model.The utility model is limited only by the claims and their full scope and equivalents.
Claims (7)
1. a kind of auxiliary device of suspension cell culture, it is characterised in that: including accessory plate, wedge-shaped transition plates, rear baffle and hand
Seat is held, the accessory plate is that it is equal to open up at least six interval with a thickness of 0.5-0.8cm for the mellow and full rectangular slab in corner on accessory plate
Wedge-shaped transition plates is installed on even peep hole, accessory plate short side right side, and rear baffle is installed in left side, and side middle position is pacified before and after accessory plate
Hand-held seat is filled, the auxiliary plate surface is equipped with lateral graduation mark and longitudinal graduation mark.
2. a kind of auxiliary device of suspension cell culture as described in claim 1, it is characterised in that: the accessory plate is transparent
The length and width of pmma material, accessory plate is identical as cell culture bags.
3. a kind of auxiliary device of suspension cell culture as described in claim 1, it is characterised in that: after the wedge shape transition plates
End is fixedly connected with accessory plate, and the thickness of rear end is identical as accessory plate, nose thickness 0.1cm.
4. a kind of auxiliary device of suspension cell culture as claimed in claim 3, it is characterised in that: before the wedge shape transition plates
The transition of end edge angle is mellow and full.
5. a kind of auxiliary device of suspension cell culture as described in claim 1, it is characterised in that: the diameter of the peep hole
It can be identical or not identical.
6. a kind of auxiliary device of suspension cell culture as described in claim 1, it is characterised in that: the auxiliary plate surface is horizontal
It is equipped with lateral graduation mark to centre bit, both ends are equipped with longitudinal graduation mark, and can be equipped with scale marker.
7. a kind of auxiliary device of suspension cell culture as described in claim 1, it is characterised in that: the hand-held seat is outside
The plate that two angles turned over are 90 °.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820967686.5U CN208517432U (en) | 2018-06-22 | 2018-06-22 | A kind of auxiliary device of suspension cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820967686.5U CN208517432U (en) | 2018-06-22 | 2018-06-22 | A kind of auxiliary device of suspension cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208517432U true CN208517432U (en) | 2019-02-19 |
Family
ID=65325586
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820967686.5U Expired - Fee Related CN208517432U (en) | 2018-06-22 | 2018-06-22 | A kind of auxiliary device of suspension cell culture |
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| Country | Link |
|---|---|
| CN (1) | CN208517432U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111979091A (en) * | 2020-07-10 | 2020-11-24 | 北京荣大咨询有限公司 | Lymphocyte extraction device and extraction method |
-
2018
- 2018-06-22 CN CN201820967686.5U patent/CN208517432U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111979091A (en) * | 2020-07-10 | 2020-11-24 | 北京荣大咨询有限公司 | Lymphocyte extraction device and extraction method |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190219 Termination date: 20190622 |
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| CF01 | Termination of patent right due to non-payment of annual fee |