CN215209499U - Pure suspension cell culture device - Google Patents
Pure suspension cell culture device Download PDFInfo
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- CN215209499U CN215209499U CN202022571530.5U CN202022571530U CN215209499U CN 215209499 U CN215209499 U CN 215209499U CN 202022571530 U CN202022571530 U CN 202022571530U CN 215209499 U CN215209499 U CN 215209499U
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- carbon dioxide
- dioxide incubator
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- suspension cell
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Abstract
The patent of the utility model relates to a pure suspension cell culture device, include: a carbon dioxide incubator for providing a closed environment required for culturing pure suspension cells; the oscillation device is fixed in the carbon dioxide incubator; and a nano silica gel adhesive plate for fixing the shake flask is arranged on the platform of the oscillating device. By adopting the pure suspension cell culture device, after the conditions of temperature, humidity and gas of the carbon dioxide incubator are adjusted and the parameters of the oscillation device are set, the oscillation device is fixed in the carbon dioxide incubator, and the pure suspension cell can be cultured by placing the cultured shake flask on the nano silica gel adhesive plate, so that the pure suspension cell culture device is simple to operate and low in manufacturing cost.
Description
Technical Field
The patent of the utility model relates to a pure suspension cell culture device.
Background
In the field of animal cell culture, most laboratories or production workshops adopt the adherent cell culture mode for cell culture, wherein the necessary equipment is a carbon dioxide incubator, but recently, with the maturity of pure suspension cell culture technology, more and more laboratories or companies begin to culture pure suspension cells, and then huge demand is generated on a carbon dioxide shaker. However, the price of the carbon dioxide shaking table produced in China is generally 7-10 ten thousand, and the price of the similar equipment imported abroad is generally 20-40 ten thousand yuan. The equipment purchasing investment is large, the purchasing period is long, and the application of a new pure suspension technology is greatly restricted.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a lower, the simple structure's of cost pure suspension cell culture apparatus, only need just can realize the cultivation to pure suspension cell through this simple structure promptly.
In order to achieve the above object, the present application provides a pure suspension cell culture apparatus, comprising: a carbon dioxide incubator for providing a closed environment required for culturing pure suspension cells; the oscillation device is placed in the carbon dioxide incubator; a nano silica gel sticking plate for sticking the shake flask is arranged on the oscillating device of the oscillating device; one part of one side plate or one part of the bottom plate of the carbon dioxide incubator is a silica gel part, a through hole for extending a power line of the oscillation device is formed in the silica gel part, and the power line is tightly matched with the through hole; the bottom surface of the carbon dioxide incubator is provided with a fixing base plate, a screw hole is formed in the fixing base plate, and the support leg of the oscillating device is fixed to the fixing base plate through a bolt matched with the screw hole.
By adopting the pure suspension cell culture device, after the conditions of temperature, humidity and gas of the carbon dioxide incubator are debugged and the parameters of the oscillation device are set during use, the oscillation device is fixed in the carbon dioxide incubator, and then the culture vessel is prevented from being adhered to the nano silica gel plate, so that the pure suspension cell culture can be started, the operation is simple, and the manufacturing cost is low.
Further, the carbon dioxide incubator is used for adherent cell culture.
Furthermore, the screw holes on the fixed substrate are uniformly distributed on the fixed substrate.
Further, the fixed substrate is an organic glass plate, and the fixed substrate is supported on the bottom surface of the carbon dioxide incubator through a support.
Further, a raised edge extending upwards and used for forming a fence is arranged on the periphery of the platform of the oscillating device, and the nano silicon adhesive plate is surrounded in the raised edge.
The present invention will be further described with reference to the accompanying drawings and the detailed description. Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description. Or may be learned by practice of the invention.
Drawings
The accompanying drawings, which form a part of the disclosure, are included to assist in understanding the disclosure, and the description provided herein and the accompanying drawings, which are related thereto, are intended to explain the disclosure, but do not constitute an undue limitation on the disclosure. In the drawings:
FIG. 1 is a schematic diagram of a pure suspension cell culture apparatus according to the present invention;
labeled as: carbon dioxide incubator 1, silica gel portion 101, oscillation device 2, power cord 201 of oscillation device, protruding edge 202, nanometer silica gel sticky board 3, fixed base plate 4, screw 401.
Detailed Description
The present invention will be described more fully with reference to the accompanying drawings. Those of ordinary skill in the art will be able to implement the invention based on these descriptions. Before the present invention is described with reference to the accompanying drawings, it is to be noted that:
the technical solutions and features provided in the present invention in each part including the following description may be combined with each other without conflict.
Moreover, references to embodiments of the invention in the following description are generally only to be considered as examples of the invention, and not as all embodiments. Therefore, all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention shall fall within the protection scope of the present invention.
With respect to the terms and units of the present invention. The term "comprises" and any variations thereof in the description and claims of this invention and the related art are intended to cover non-exclusive inclusions.
Referring to fig. 1, a pure suspension cell culture device comprises a carbon dioxide incubator 1 and a shaking device 2, wherein the carbon dioxide incubator 1 is used for providing a closed environment required for culturing pure suspension cells, and the shaking device 2 is fixed in the carbon dioxide incubator 1; and a nano silica gel sticky plate 3 for adhering the shake flask is arranged on the platform of the oscillating device 2. The side plates of the carbon dioxide incubator 1 are transparent glass/organic glass plates, so that the direct observation of the condition inside the carbon dioxide incubator 1 is facilitated. One part of one side plate or the bottom plate of the carbon dioxide incubator 1 is a silica gel part 101, a through hole for extending a power line 201 of the oscillation device 2 is arranged on the silica gel part 101, and the power line 201 is tightly matched with the through hole. Thus, the power supply separation of the carbon dioxide incubator 1 and the oscillation device 2 is realized, and the control of the equipment is more flexible. The bottom surface of the carbon dioxide incubator 1 is provided with a fixing substrate 4, the fixing substrate 4 is provided with a screw hole 401, and the support leg of the oscillation device 2 is fixed on the fixing substrate 4 through a bolt matched with the screw hole 401. The screw holes 401 on the fixed base plate 4 are uniformly distributed on the fixed base plate 4. Through setting up foretell fixed baseplate 4, realize carrying out nimble fixed to oscillation device 2 different positions, still can adopt the oscillation device 2 of different specifications to set up it in carbon dioxide incubator 1 simultaneously. The fixed substrate 4 is an organic glass plate, and the fixed substrate 4 is supported on the bottom surface of the carbon dioxide incubator 1 by a support to prevent the fixed substrate 4 from directly contacting the bottom plate of the carbon dioxide incubator 1. The platform of oscillation device 2 is provided with the protruding edge 202 that upwards extends, is used for forming the rail along the week, nanometer silica gel sticky board 3 encloses in this protruding edge 202 to convenient the setting position who confirms nanometer silica gel sticky board 3 carries on spacingly with preventing nanometer silica gel sticky board 3 simultaneously.
During assembly and installation, the pure suspension cell culture device can be set by referring to the following operations, the device can be modified according to equipment on the market at present, for example, after purchasing the carbon dioxide incubator 1, all partition boards inside the carbon dioxide incubator 1 are removed, a 25 mm-gauge or 50 mm-gauge oscillator is purchased to be used as a vibrating device (the specific model depends on the requirement of cells planned to be cultured, the 25 mm-gauge oscillator is generally selected), the panel size requirement is slightly smaller than the inner cavity size of the carbon dioxide incubator 1, and the carbon dioxide incubator 1 can be placed in. The oscillation device 2 is placed in the carbon dioxide incubator 1, a silica gel part 101 is provided on the rear side plate of the carbon dioxide incubator 1 and a hole is punched in the silica gel part 101, and the power cord 201 of the oscillation device 2 is passed out from the punched silica gel plug while maintaining the sealing of the carbon dioxide incubator 1.
If the purchased oscillation device 2 is provided with a part for placing a vessel, such as a stainless steel bracket, the stainless steel bracket on the oscillation device 2 can be detached, then the nano silica gel adhesive plate 3 is installed, the nano silica gel adhesive plate 3 can be directly placed on a platform of the oscillation device 2 after the bracket is detached, and the periphery of the platform is provided with a convex edge 202 for limiting the nano silica gel adhesive plate 3. After the temperature of the incubator and the rotation speed of the shaking apparatus 2 are calibrated, the carbon dioxide shaker can be used to culture pure suspension cells.
This pure suspension cell culture device simple structure (through the transformation of current equipment can, the transformation is simple, non professional can accomplish the repacking through simply dismantling the installation), the input cost is low, for example, repacking a SANYO MCO 150's carbon dioxide incubator 1, only need invest about 1200 yuan can accomplish the repacking, and purchase a new carbon dioxide incubator 1 and need spend at least 8-40 ten thousand yuan and just can reach similar effect. The investment for purchasing new equipment can be greatly reduced, the existing equipment can be utilized to the maximum extent, the time for purchasing the equipment again due to the improvement of the process technology is saved, a large amount of capital investment can be saved, and a good using effect is achieved.
The device has simple structure, and the refitting operation of the existing equipment is quicker than purchasing new equipment, and the consumed time is short. The procurement of the parts and the refitting of the refitting process only take about 10 days, while the procurement of the equipment takes 1-3 months.
This device adopts nanometer silica gel's stickness pad as fixing device, and with traditional carbon dioxide shaking table adoption stainless steel fixed bolster comparison, it is more convenient to use, can more effectual utilization current area.
The contents of the present invention have been explained above. Those of ordinary skill in the art will be able to implement the invention based on these descriptions. Based on the above-mentioned contents of the present invention, all other embodiments obtained by those skilled in the art without creative efforts shall fall within the protection scope of the present invention.
Claims (5)
1. A pure suspension cell culture device, comprising:
a carbon dioxide incubator for providing a closed environment required for culturing pure suspension cells;
the oscillation device is placed in the carbon dioxide incubator;
a nano silica gel sticking plate for sticking the shake flask is arranged on the oscillating device of the oscillating device;
one part of one side plate or one part of the bottom plate of the carbon dioxide incubator is a silica gel part, a through hole for extending a power line of the oscillation device is formed in the silica gel part, and the power line is tightly matched with the through hole;
the bottom surface of the carbon dioxide incubator is provided with a fixing base plate, a screw hole is formed in the fixing base plate, and the support leg of the oscillating device is fixed to the fixing base plate through a bolt matched with the screw hole.
2. The pure suspension cell culture device of claim 1, wherein the carbon dioxide incubator is a carbon dioxide incubator for adherent cell culture.
3. The device for culturing suspension-only cells of claim 1, wherein the screw holes of the fixing substrate are uniformly distributed on the fixing substrate.
4. The pure suspension cell culture device according to claim 1, wherein the fixed substrate is a plexiglass plate, and the fixed substrate is supported on the bottom surface of the carbon dioxide incubator by a support.
5. The pure suspension cell culture device of claim 1, wherein the platform of the oscillation device is provided with a raised edge extending upwards for forming a fence, and the nano silicon adhesive plate is enclosed in the raised edge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022571530.5U CN215209499U (en) | 2020-11-09 | 2020-11-09 | Pure suspension cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022571530.5U CN215209499U (en) | 2020-11-09 | 2020-11-09 | Pure suspension cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215209499U true CN215209499U (en) | 2021-12-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022571530.5U Active CN215209499U (en) | 2020-11-09 | 2020-11-09 | Pure suspension cell culture device |
Country Status (1)
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| CN (1) | CN215209499U (en) |
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2020
- 2020-11-09 CN CN202022571530.5U patent/CN215209499U/en active Active
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Legal Events
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 610100 No. 358, lingchi street, Chengdu Economic and Technological Development Zone, Sichuan Province Patentee after: Chengdu Shiji biopharmaceutical Co.,Ltd. Patentee after: Shiji Biotechnology (Nanjing) Co.,Ltd. Address before: No.358, lingchi street, Chengdu Economic and Technological Development Zone, Sichuan 610000 Patentee before: CHENGDU TECBOND BIOLOGICAL PRODUCTS CO.,LTD. Patentee before: Shiji Biotechnology (Nanjing) Co.,Ltd. |
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| CP03 | Change of name, title or address | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20221202 Address after: No.358, lingchi street, Chengdu Economic and Technological Development Zone, Sichuan 610000 Patentee after: Chengdu Shiji biopharmaceutical Co.,Ltd. Address before: 610100 No. 358, lingchi street, Chengdu Economic and Technological Development Zone, Sichuan Province Patentee before: Chengdu Shiji biopharmaceutical Co.,Ltd. Patentee before: Shiji Biotechnology (Nanjing) Co.,Ltd. |
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| TR01 | Transfer of patent right |