CN206512229U - It is a kind of to be used for the multifunctional bio reactor of cell culture and cell sorting - Google Patents

Abstract

The utility model is related to a kind of for cell culture and the multifunctional bio reactor reaction device of cell sorting.The reactor includes turning device, cell culture container, turning device includes the turnover bracket that can be operated around spatial axes, with the field generator for magnetic of turnover bracket physical connection, cell culture container is removably fixed on turnover bracket, cell culture container is located on above-mentioned spatial axes or its extended line, turnover bracket is followed to be operated according to certain rules with track, cell culture container end face is close to field generator for magnetic, the controllable change around cell culture container periphery and internal magnetic field of field generator for magnetic.The utility model carries out the co-cultivation of adherent cell and suspension cell in the case where being changed without container, agitation inside nutrient solution is realized by motion of the wherein carrier under magnetic field environment, reduce the risk of cell contamination and target cell damage and death, and the culture efficiency of cell can be drastically increased simultaneously as the carrier of attached cell.

Description

It is a kind of to be used for the multifunctional bio reactor of cell culture and cell sorting

Technical field

The utility model is related to a kind of biotechnology equipment, is relevant with cell sorting with cell culture multi-functional Bioreactor.

Background technology

With the development of science and technology, modern cell biology engineering technology has been widely used for the Fang Fang of social production Face face.And along with cell biology engineering technology is in the fast development of social multiple industries such as biology, medical treatment, beauty and answers With in the urgent need to carrying out large-scale cell culture, to obtain a large amount of useful cell expression products.And use vial quiet Put or revolving bottle cultural method, its cell quantity cultivated and its secretory product can not meet the need of social every profession and trade already Ask.By technical research and development for many years, polytype bioreactor, roller bottle formula biological respinse have been developed so far Device, agitation suspension culture bioreactors, shake pocket type bioreactor, rotating wall vessel bioreactor, plate biological respinse Device, fixed fluidized bed formula bioreactor etc..

In the mode of culture cell, there are static condition and motion state two ways.Cultivated under static condition Cell, the shearing force being subject to during culture is minimum, but the cell cultivated under static condition is when density is larger, easily causes in culture Some cells of container bottom can not obtain enough nutrition, and along with lactic acid, pyruvic acid in cell cultivation process And the gradually increase of some metabolic wastes for suppressing growth, easily cause some cell growth arrests of bottom of culture vessel very To necrosis.Therefore large-scale cell culture and amplification are not suitable for.In the application that cell is cultivated under motion state, have perhaps Many bioreactors make cell using the cell inside nutrient solution in mechanical agitation type or airlift agitation formula agitation bioreactor It is in suspended state., can be in cell table when being stirred in traditional mechanical agitation type or the bioreactor of airlift agitation formula Face produces shearing force.When cultivating cell, when particularly cultivating the stem cell of quite sensitive, excessive shearing force can cause cell Damage it is even dead.In some bioreactors, pass through standing or soft motion mode culture cell, although reduction Shearing force, but once shut down, cell may accumulate on its in cell culture container bottom or cell culture container Its position, and be unevenly to be distributed, it is unfavorable for most cell growths.

A bioreactor design scheme that can be suitably used for micro-carriers cell culture is proposed in patent 102199538A, Allow microcarrier suspension by way of allowing tank body upper and lower side to exchange repeatedly, adsorb the adherent cell on microcarrier with tank body The suspension of cell and microcarrier is moved up and down and realizes, and in actual application, microcarrier is with the upper downward of tank body During changing, it is intended to the lateral movement close to culture tank wall, opposite side is compared in the agitation that the culture medium, cell to side are produced Greatly, the agitation that opposite side is subject to is limited, so that cell even suspension in whole culture environment can not be made, in this process, The cell of the more violent side of agitation is highly prone to damage so that cell mortality increase.

In cell injuring model, there is the cell that suspension cell grows by different way with two kinds of attached cell.It is adherent Cell needs to depend on certain support surface and could grown, when the growing multiplication of cell occupies whole support surfaces Afterwards, cell is unable to continued growth due to contact inhibition, therefore in support surface accumulates what is limited for the cell of adherent growth In the case of be difficult carry out large-scale culture.And most of bioreactors can only meet the cell of one of which growth pattern Culture.And in large-scale culture cell, it is necessary to different culture cells be separated from culture vessel, because some In, such as during stem-cell therapy, in mescenchymal stem cell and candidate stem cell co-incubation, the growth conditions of cell are more Good, therapeutic effect is also more protruded.And in order to allow the cell of adherent growth can be with highdensity growth, just generating makes cell exist The technology of suspension growth on microcarrier：Attached cell cell attachment is allowed on microcarrier, when microcarrier suspension in the medium when, Cell suspension growth together, because the quantity of microcarrier is more, surface area big, the surface that can be grown for cell is also quite big, can be with Meet the extensive fast-growth of cell.

In present conventional microcarrier bioreactor, the size for the minichromosome vector that attached cell is typically adsorbed is several micro- Rice to hundreds of microns, such as patent CN 101416059A (hydrogel patent) when microcarrier is smaller, connected microcarrier it Between space it is fewer, the density ratio of cell growth is larger, and it is not enough to easily cause oxygen supply, causes cell death.When cell increases After certain amount, these cells and microcarrier can assemble agglomerating together, form aggregation, the cell inside aggregation can not Sufficient nutrient is obtained, growth conditions will be deteriorated, if stem cell, then be more prone to non-specific differentiation.And thin After born of the same parents' culture shaping, it may be necessary to cell is washed out from niche using certain special program, or must be added Enter the material that certain specific reagent degraded builds niche, to discharge target cell.

, all there is some defects and limitation in various kinds of cell training method enumerated above, it is impossible to efficiently in principle Rate, it is extensive, multifarious realize the culture of all kinds cell, so as to influence cellular biological technique socially scale Extensive use.

Utility model content

For some existing socially wide variety of bioreactors, on cell culture technology and method, it is desirable to take Breakthrough development and scale application, in addition it is also necessary to solve some following problems：

1., enough nutrition supply space：During cell growth, enough by continual supply Nutrient, and be with enough space in cell week and carry out the exchange of nutrient and the excretion of secretion.

2., gentle soft agitating mode：In cell cultivation process, reduce as far as possible because to culture medium or carrier The shearing force for stirring and being produced in cell surface, it is to avoid the spontaneous differentiation of the damage of cell, particularly stem cell.

3., continual and steady suspension technology：, it is necessary to ensure that what suspension cell can be continual and steady hangs in culture cell processes Float over free growth in culture vessel.

4., the culture of multiple types cell：A kind of mode for being capable of high-efficient culture cell, can either cultivate suspension cell, Attached cell can be cultivated simultaneously.Such as candidate stem cell and the Combined culture of mescenchymal stem cell.

5., easily detect real-time：In cell cultivation process, it is possible to achieve efficiently detect cell real-time Growing state, and culture medium situation.

6. synchronous sorting, can be realized in a straightforward manner：Cell sorting can be allowed to expand two kinds of programs same with cell Completed in individual culture vessel, it is easy to operation, the wind of pollution and the damage of target cell and death can be reduced to greatest extent Danger, so as to be greatly enhanced the efficiency of cell culture.

7., easy cell harvesting method:Other material need not be added, makes micro-loop of the cultured cell from appendix Spun off in border.

8. man-machine interactive operation system, multi-functional, easy to operate：Whole computer control record, Real-time Feedback and regulation Systematic parameter.

Only meet function above requirement bioreactor, could in social each field, the practical application of every profession and trade, It can quickly, on a large scale, widely grow up, and quickly produce economic benefit and social effect, to promote cell biological section The sustainable development of technology and research provides power and basis.

A kind of multifunctional bio reactor to cultivate cell and cell sorting involved by the utility model, is with simulation The dynamic microenvironment of human inner cell's growth, in the case where reducing shearing force, the efficient all types of cells of amplification, such as hematopoiesis Stem cell, immunocyte etc., and ensure " dryness " of the activity of cell, particularly stem cell.Meanwhile, biology of the present utility model Reactor is integrated with a variety of functions such as full automatic cell sorting, cell density detection feedback, can allow cell sorting and cell Amplification two kinds of flows is completed in a culture vessel, it is easy to operation, can reduce to greatest extent cell contamination with Target cell damages and dead risk, drastically increases the culture efficiency of cell.

Involved a kind of multifunctional bio reactor to cultivate cell and cell sorting of the present utility model mainly leads to Following technical scheme is crossed to realize：Including turning device, cell culture container, turning device includes what can be operated around spatial axes Turnover bracket, the field generator for magnetic with turnover bracket physical connection, cell culture container is removably fixed on upset On support, cell culture container is located on above-mentioned spatial axes or its extended line, follows turnover bracket according to certain rules and track Operating, cell culture container end face is close to field generator for magnetic, and field generator for magnetic is controllable to surround cell culture container periphery And the change of internal magnetic field.

Above-described turning device it is main by pedestal, motor cover, electric machine support, upset motor, turnover bracket, magnetic core, The parts such as seal gasket are constituted.Upset motor is fixed on electric machine support, and motor cover covers in upset motor and pushes down fluid sealant Pad is fixed on electric machine support；Magnetic core is arranged in turnover bracket；Turnover bracket is enclosed in upset motor to be fixed with electric machine support On pedestal.Characterized in that, described upset motor can be set according to program parameter presses certain rotating speed, frequency and positive and negative two Rotated to angle.

Above-described cell culture container is mainly made up of parts such as container framework, carriers.Carrier passes through container framework End interface be positioned over inside container framework.Characterized in that, described cell culture container is accommodated by container framework The cell culture key element such as nutrient solution, carrier, cell, to provide the microenvironment of cell growth.And with turning device rotation and The up and down motion of carrier in container framework is driven, so that the function of nutrient solution in agitation vessel framework is realized, it is thin in favor of suspending Born of the same parents' is uniformly distributed growth.And a surface that can be grown is provided for attached cell by carrier simultaneously, to expand attached cell Growing space.

Above-described multifunctional bio reactor can include control system, and it is mainly by mainframe box, display screen, master control The parts such as device processed, Switching Power Supply, cabinet cover plate are constituted.Display screen is fixed on the front end face of mainframe box, and master controller is with opening the light Power supply is arranged on the dividing plate inside mainframe box, and cabinet cover plate is fixed on mainframe box rear end face.Characterized in that, being responsible for whole system The processing of the human-machine interactive informations such as interface display, operation, program operation and the setting of system.And will set from master controller Program or command information are converted into electronic signal, and then manipulate the work of electric component in whole bioreactor, and will be from The feedback of the information that controlled electric component is obtained is to master controller and shows on the display screen.Described electric component includes upset electricity Machine, magnetic core, photoelectric detector, peristaltic pump etc.

One or more cell culture containers can be equipped with the turnover bracket of above-described turning device.

The motion mode of above-described turnover bracket includes but is not limited to overturn, waved, parabolic motion, curvilinear motion Or move along a straight line up and down.

Above-described turnover bracket two side ends are provided with magnetic core.Magnetic core can be made up of electromagnet or permanent magnet.Magnetic When core uses permanent magnet, cooperation is equipped with magnetic core support and shielding magnetic sheet.Magnetic sheet will be shielded and magnetic core is arranged on magnetic core support Two sides, the presence or absence of magnetic force is realized by the rotation of magnetic core support；When magnetic core uses electromagnet, pass through the big of control electric current Small, direction and break-make control the size of magnetic force, direction and whether there is, and the function of absorption or release appendix body is reached with this, So as to realize different existences of the appendix body in container framework.

Above-described magnetic core be permanent magnet constitute when, magnetic core support is rotated by magnetic core motor control, rotary speed be 1~ 200 r/min, its optimal rotary speed is 20-60r/min or so.

Above-described magnetic core and the distance of container framework both ends of the surface are no more than 200 millimeters.

Above-described shielding magnetic sheet can be by the magnetic conductivity such as permalloy, silicon-iron soft magnetic alloy and iron, cobalt, nickel and its alloy High metal material is made.

Upset motor described above is especially used including but not limited to dc motor, asynchronous motor, synchronous motor In the stepper motor and servomotor that accurately control.

The flip angle of upset motor described above turns clockwise no more than 360 °, and rotate counterclockwise is no more than 360 °.

The rotary speed of upset motor described above is 1~200r/min, and its optimal rotary speed is 2~60r/min Left and right.

Photoelectric detector can be equipped with above-described turnover bracket, its include photo resistance and illuminator and Various photoelectric detection probes.

The condition of above-described photoelectric detector monitoring includes but is not limited to：Cell density (concentration), pH value, grape Temperature, humidity in sugar, carbon dioxide, oxygen, nitrogen gas concn and reactor chamber etc..

Above-described photoelectric detector is plugged on turnover bracket by electrode contact pin mode；Or neck card can be passed through Button mode is snapped onto on turnover bracket；Or can be fixed by welding on turnover bracket；Or upset branch can be fixed on by latch On frame；Or can be fixed on screw on turnover bracket；Or can be by magnetic absorption on turnover bracket, and its interface reality can be passed through The functions such as existing conductive, transmission data.

Above-described seal gasket is mainly by silica gel, rubber, polytetrafluoroethylene (PTFE), graphite, glass fibre or carbon fiber Shaping is made Deng material.

The above-described main material of pedestal is made by metal, plastics or other nonmetallic materials and is molded.It is optimal to use Medical stainless steel and polyformaldehyde (POM), polypropylene (PP), makrolon (PC), polyethylene (PE), polystyrene (PS), poly- four PVF (PTFE), polymethyl methacrylate (PMMA), acrylonitrile-butadiene-styrene copolymer (ABS) etc..

The above-described main material of motor cover is made by metal, plastics or other nonmetallic materials and is molded.Most preferably adopt With medical stainless steel and polyformaldehyde (POM), polypropylene (PP), makrolon (PC), polyethylene (PE), polystyrene (PS), poly- Tetrafluoroethene (PTFE), polymethyl methacrylate (PMMA), acrylonitrile-butadiene-styrene copolymer (ABS) etc..

Above-described turnover bracket is mainly made by plastics, metal or other nonmetallic materials and is molded.Most preferably adopt With medical stainless steel and polyformaldehyde (POM), polypropylene (PP), makrolon (PC), polyethylene (PE), polystyrene (PS), poly- Tetrafluoroethene (PTFE), polymethyl methacrylate (PMMA), acrylonitrile-butadiene-styrene copolymer (ABS) etc..

The above-described main material of mainframe box is molded by sheet metal, plastics or other nonmetallic materials are fabricated to Type.

Can integrated built-in CO in above-described mainframe box₂Box function is cultivated, and turning device and cell culture can be held Device integrated installation is in built-in CO₂In incubator.Above-described mainframe box is including but not limited to CO₂Institute contained by incubator It is functional.

Above-described display screen can use non-touch display screen and touching display screen.

On above-described mainframe box can be set have for external device access communication and power data line interface, comprising but It is not limited to the interface types such as USB interface, coaxial interface, optical fiber interface, cable interface, fire-wire interfaces, serial line interface.

It is above-described can separately on mainframe box the peripheral hardware such as external add-in keyboard, mouse, board, scanner type input it is defeated Go out equipment.

Charge pump can be installed on above-described mainframe box, it is characterised in that for cell culture container inject nutrient solution or The cells such as other growth factors, hormone, cell factor breed required nutrition and provide lasting perfusion power, its type The institute included including but not limited to peristaltic pump or syringe or compression pump or piston pump etc. is functional.

Boom support can be equipped with above-described mainframe box, so as to which the nutrient solution storage container of Large Copacity is mounted on Above, long-time, the cell culture perfusion demand of large volume can be achieved.

Its course of work of above-described charge pump is to manipulate charge pump by program run by master controller, and passes through phase Fresh nutrient solution is filled into cell culture container by the pipeline answered, with this cultivated cell of rapid amplification and differentiation, and Nutrient solution waste liquid and discarded object are discharged from culture vessel, while ensureing that the cell of propagation is deposited in culture vessel.

Above-described nutrient solution storage container its including but not limited to existing packed memory, glass container store The storage container that device, stainless cylinder of steel body memory and other metals, plastics or nonmetallic materials are made.

Above-described nutrient solution storage container can use one；Or two can be used；Or two or more can be used, according to Different fostering requirements and environment install the nutrient solution storage container of varying number.

Above-described pipeline is including but not limited to silicone tube, stainless steel tube, plastic tube and other nonmetallic pipes being made Road.

For the wide variety of bioreactor for cell culture in the market, needed greatly in face of zooblast When scale, High Density Cultivation, also to ensure to be reduced to minimum to the degree of injury of cell during cell culture simultaneously, just It is for current technology level or highly difficult.Since under current technology background, damage of the cell in incubation Essentially from two aspects：One when being introduced into gas nutrient, and the bubble that gas is formed when escaping culture medium liquid level produces quick-fried Split, so as to cause damage to cell, two be that when using mechanical agitation, the shearing force produced inside medium liquid is made to cell Into damage.

Bioreactor of the present utility model is exactly based on this 2 points, fundamentally to solve the two problems.And Possess some following advantage on theory structure：

1., in structure, the growth of culture vessel inner cell is to keep suspending or static condition or suspension in cell Carried out during with static condition over-over mode.One side culture vessel is ventilative, and gas is that slowly infiltration is dissolved into culture medium , whole process is importing directly into culture medium without gas, and the explosion that would not also generate bubble and its generation causes damage to cell； On the other hand in cell growth, slowly moved using carrier self gravitation or buoyancy or magnetic, to reach stirring training The effect of base is supported, so that shearing force will not be produced inside medium liquid, mechanical stirring side is also just fundamentally solved The damage that formula is caused to cell.And bioreactor of the present utility model uses batch culture mode, is adding cell While growth stability, cell and carrier can be also set to control its suspension effect in stirring, it is thin so as to preferably build The swapace of the microenvironment of intracellular growth and its nutrition.

2., in principle, the controlling magnetic field at culture vessel two ends can be used for coordinating cell sorting procedures and culture program.Carefully When born of the same parents sort, controlling magnetic field is used to capture the cell that carrying magnetic is marked in sorting cell, and allows these cell adherences in above-mentioned The surface of carrier, therefore can just allow these cell continued growths captured to be bred after other cells have been isolated.Simultaneously also because To eliminate the step for sorting and shift under external status as other culture vessels, microenvironment is reduced to thin The pollution of born of the same parents.

3., functionally, culture vessel can be used for the culture of most cells, including suspension cell, attached cell and Facultative attached cell.And for suspension cell, due to inside the nutrient solution of culture vessel without shearing force, can be with maximum limit The reduction of degree causes candidate stem cell non-specificity differentiation.

4., in performance, the growth of cell is easy to operate by computer program control in culture vessel, and cell is in sorting With can monitor condition of culture in growth course at any time, and pass through photoelectric detector and obtain data parameters, record and reporter cell Growing state, so as in real time provide supplementing culture medium, change culture medium or stop culture suggestion.In supplementing culture medium When, bioreactor automatically begins to exchange culture medium including emptying, cleaning and filled.

5., in utilization, culture vessel, without cleaning, sterilization, will not produce intersection dirty due to being to use medical disposable material Dye, the container inner wall that cells contacting is arrived is safe and reliable, and the making of culture vessel can realize high-volume, the life of scale Production, yield and quality are easier to ensure that, can meet the demand of mass cell culture.

6., in harvest, after the completion of cell culture, cell may need to use special program to be washed from niche Go out.Other bioreactors need to melt or the material of niche are built with enzymic digestion, to discharge target cell. And in bioreactor of the present utility model, the space between carrier naturally form the small Tiny ecosystem ring for cell growth Border, and when conversion magnetic field intensity, when load is risen or is sunk, it is possible to discharge target cell easily.

Bioreactor involved by the utility model, can be with maximum limit due to improving agitating mode during cell culture Infringement of the shearing force to cell during the reduction cell growth of degree caused by liquid agitation is moved, can be in cell cultivation process It is middle that enough Metabolic support is provided, and can carry out simultaneously all types of cells particularly sensitive cell propagation and cell it is multiple Culture, and there is provided letter for the optimization of the cell culture key element such as synchronization sorting, the real-time monitoring of cell culture environment to cell Just, efficient method, greatly improves the culture efficiency of cell.

Brief description of the drawings

Fig. 1 is the utility model ZYX bioreactor M1 dimensional structure diagrams.

Fig. 2 is the utility model ZYX bioreactor M1 decomposition texture schematic diagrams.

Fig. 3 is the utility model ZYX bioreactor M1 smart host control system decomposing schematic representations.

Fig. 4 is that the utility model ZYX bioreactor M1 multifunctional turnovers transfer from one department to another decomposing schematic representation of uniting.

Fig. 5 is the utility model ZYX bioreactor M1 cell culture system decomposing schematic representations.

Fig. 6 A-L are the utility model ZYX bioreactor M1 cell culture circulation process decomposing schematic representations.

Fig. 7 is the utility model ZYX bioreactor S20 dimensional structure diagrams.

Fig. 8 is the utility model ZYX bioreactor S20 decomposition texture schematic diagrams.

Fig. 9 is the utility model ZYX bioreactor S20 smart host control system decomposing schematic representations.

Figure 10 is that the utility model ZYX bioreactor S20 multifunctional turnovers transfer from one department to another decomposing schematic representation of uniting.

Figure 11 is the utility model ZYX bioreactor S20 cell culture system decomposing schematic representations.

Figure 12 A-L are the utility model ZYX bioreactor S20 cell culture circulation process decomposing schematic representations.

Figure 13 is the utility model ZYX bioreactor cell culture container interface diagrams.

Figure 14 is that the multifunctional turnover of the utility model ZYX bioreactors transfers from one department to another the signal being placed in CO2 incubators of uniting Figure.

Embodiment

Embodiment one：

ZYX bioreactors M1 of the present utility model stereochemical structure such as Fig. 1, is mainly made up of, such as three module sections Shown in Fig. 2.Constituted including control system 1, turning device 2 and cell culture container 3 etc..

As shown in figure 3, in the controls, comprising：Mainframe box 1-1, display screen 1-2, warning lamp 1-3, panel 1-4, Computer mainframe switch 1-5, boom support 1-6, waste fluid bag 1-7, culture medium storage bag 1-8, code reader antenna 1-9, suspension rod positioning Ring 1-10, silica gel catheter 1-11, master controller 1-12, mainframe box bonnet 1-13, solidus groove 1-14, first switch power supply 1-15, Second switch power supply 1-16, installing plate 1-17, host computer 1-18, main frame fixed plate 1-19, radiator fan 1-20, mainframe box electricity It is source switch 1-21, protection switch 1-22, power interface 1-23, cable interface 1-24, conduit end cap 1-25, beam 1-26, compacted Dynamic pump 1-27, mainframe box protecgulum 1-28, usb 1-29, the first communications line terminal 1-30.In overall assembling process, first will Four beam 1-26 screw locks are in mainframe box 1-1 bottoms；Usb 1-29, the first communications line terminal 1-30 are used respectively Screw is fixed in the upper lower bayonet slot on the right side of mainframe box 1-1；Host computer 1-18 is screwed by main frame fixed plate 1-19 In mainframe box 1-1 bottom；Mainframe box power switch 1-21, protection switch 1-22, power interface 1-23 and cable interface 1-24 It is screwed respectively in the corresponding notch of mainframe box 1-1 rear portions lower end；Radiator fan 1-20 is fixed on mainframe box 1-1 The relative hole of left upper part in；Two peristaltic pump 1-27 are each passed through the corresponding bayonet socket in mainframe box 1-1 front ends and are screwed； Display screen 1-2 correspondences are screwed in mainframe box 1-1 upper ends, and warning lamp 1-3 and computer mainframe switch 1-5 are fixed respectively In port on panel 1-4, then the panel 1-4 assembled is covered into display screen 1-2 be fastened on screw on mainframe box 1-1 End；By code reader antenna 1-9 screw locks in boom support 1-6 sides, and power line is passed through on boom support 1-6 Hole is connected in mainframe box 1-1, then two suspension rod locating ring 1-10 are respectively fitted over into boom support 1-6 two ends, then will be hung Bar support 1-6 two ends pass through the hole slot of mainframe box 1-1 upper ends, are fixed inside mainframe box 1-1 with nut check；By solidus groove 1-14, first switch power supply 1-15, second switch power supply 1-16 and master controller 1-12 are screwed in installing plate 1- respectively On 17, then it is screwed on the supporting plate in mainframe box 1-1 together with installing plate 1-17, and respectively by other electrical equipment The connection corresponding with data wire of the power line of element or grafting are got up；Mainframe box protecgulum 1-28 is connected to mainframe box 1-1 by bearing pin On anterior hinge, then with screw mainframe box bonnet 1-13 is fixed on mainframe box 1-1 rear portions；By waste fluid bag 1-7 and culture medium Storage bag 1-8 is hung on the suspension hook on boom support 1-6, and the identification label on culture medium storage bag 1-8 is faced Code reader antenna 1-9, to read at any time or data storage；Two silica gel catheter 1-11 are connected to waste fluid bag 1-7 respectively again On the port of culture medium storage bag 1-8 bottoms, and through the notch of mainframe box 1-1 left upper portions, it is stuck in two wrigglings In pump 1-27, drawn by the hook on mainframe box 1-1, conduit end cap 1-25 is covered fixed silica gel catheter 1-11, with this group Dress up a complete control system.

As shown in figure 4, in turning device, comprising：Second communications line terminal 2-1, motor cover 2-2, upset motor 2-3, Motor support plate 2-4, locating sealing ring 2-5, rear end support 2-6, motor shaft sleeve 2-7, rollover stand bonnet 2-8, magnetic core motor 2- 9th, first electrode set 2-10, turnover bracket 2-11, pedestal 2-12, Photoelectric Detection bracket cover 2-13, Photoelectric Detection support 2-14, light Electro-detection receiver 2-15, magnetic core 2-16, magnetic core support 2-17, front-end bracket 2-18, drive end bearing bracket 2-19, turnover bracket protecgulum 2-20, second electrode set 2-21, magnetic core barricade 2-22, locking knob 2-23, Photoelectric Detection transmitter 2-24, catheter holder 2-25, conduit fix lid 2-26.Rear end support 2-6 is fixed on pedestal 2-12；Upset motor 2-3 is screwed in motor branch On fagging 2-4, then motor support plate 2-4 is screwed on rear end support 2-6；Respectively by first electrode cover 2-10 and Second electrode set 2-21 is arranged in the middle of turnover bracket 2-11 in the cylindrical drum space at two ends, and four locking knob 2-23 are distinguished In the corresponding fixing hole in turnover bracket 2-11 upper ends four, two magnetic core motor 2-9 are screwed respectively and turned over On pivoted frame bonnet 2-8, two pieces of magnetic core 2-16 and two pieces of magnetic core barricade 2-22 are relatively fixed at magnetic core support 2- two-by-two respectively 17, then assemble two magnetic core support 2-17 one end necks are stuck in magnetic core motor 2-9 rotary shaft respectively, two magnetic cores Position is relative after two pieces of magnetic core barricade 2-22 assemblings on support 2-17, then two sleeves on turnover bracket 2-11 point Not Chuan Guo two magnetic core support 2-17 assembled be connected on rollover stand bonnet 2-8, fixed with screw lock, then upset Two holes on support protecgulum 2-20 are respectively fitted over two magnetic core support 2-17 shaft end, are nested together with turnover bracket 2-11, And fixed with screw lock；Motor shaft sleeve 2-7 is screwed on the turnover bracket 2-11 assembled, and in turnover bracket Locating sealing ring 2-5 is put in the rotating shaft at 2-11 two ends respectively, is then respectively fitted over rear end support 2-6's and front-end bracket 2-18 In axis hole, and the shaft hole sleeve on motor shaft sleeve 2-7 is locked in upset motor 2-3 rotating shaft with holding screw, and in upset Support 2-11 front end has been screwed drive end bearing bracket 2-19；Second communications line terminal 2-1 is screwed in motor cover 2- On 2 in corresponding notch, and the second corresponding power line of communications line terminal 2-1 tail ends or data wire be connected to corresponding Magnetic core motor 2-9 and first electrode are covered on 2-10, then motor cover 2-2 is separately fixed at rear end support 2-6 and pedestal with screw On 2-12；Finally catheter holder 2-25 is screwed on motor cover 2-2, and conduit is fixed lid 2-26 and is stuck in conduit branch In frame 2-25 necks；Photoelectric Detection receiver 2-15 is screwed on Photoelectric Detection support 2-14, and covers photoelectricity inspection Survey bracket cover 2-13 be screwed, be assembled into Photoelectric Detection receiving terminal, by correspondence be plugged on turnover bracket 2-11 lower ends First electrode covers 2-10 and second electrode set 2-21 front and rear electrode card is intraoral；Photoelectric Detection transmitter 2-24 is consolidated with screw It is scheduled on Photoelectric Detection support 2-14, and covers Photoelectric Detection bracket cover 2-13 and be screwed, is assembled into Photoelectric Detection transmitting End, by correspondence be plugged on turnover bracket 2-11 lower ends first electrode cover 2-10 and second electrode set 2-21 front and rear electrode card It is intraoral, a complete turning device is assembled into this.

As shown in figure 5, in cell culture container, including container framework 3-1, bead inlet port closure 3-2, carrier magnetic bead 3-3. By a number of carrier magnetic bead 3-3 from container framework 3- after bead inlet port closure 3-2 rotations are opened from container framework 3-1 Bead inlet port on 1 is put into the space inside container framework 3-1, then bead inlet port closure 3-2 is tightened on into container framework 3-1 Bead inlet port on seal, a complete cell culture container is assembled into this.

As shown in figure 1, the two silica gel catheter 1-11 drawn in above control system are passed through on catheter holder 2-25 Groove, is respectively connected on container framework 3-1 on corresponding interface, and A/C fixes lid 2-26, it is ensured that from culture medium storage The silica gel catheter 1-11 that bag 1-8 is picked out is connected to the input on container framework 3-1, the silica gel catheter 1- picked out from waste fluid bag 1-7 11 are connected to the outlet side on container framework 3-1, then cell culture container 3 is fixed on turning device 2 with locking knob 2-23 Turnover bracket 2-11 frame slot in, thus constitute a complete ZYX bioreactors M1.

ZYX bioreactors M1 workflow is as follows：

Referring to Fig. 3,4,5, according to the program woven in control system, command information is converted into by master controller, controlled Turning device processed is acted by command information.After control system is powered and started, it is necessary to logical before the Cell culture procedures that bring into operation The master controller 1-12 that crosses in control system, initial detecting each electric system operation it is whether normal.Include display screen 1- 2nd, warning lamp 1-3, code reader antenna 1-9, first switch power supply 1-15, second switch power supply 1-16, radiator fan 1-20, wriggling Pump 1-27, the first communications line terminal 1-30, the second communications line terminal 2-1, upset motor 2-3, magnetic core motor 2-9, Photoelectric Detection The parts such as receiver 2-15, Photoelectric Detection transmitter 2-24.If a certain part therein is problematic, runs abnormal or do not have Action, then alarm lamp occurs flicker, and the buzzer on warning lamp 1-3 sounds the alarm.Wherein display screen 1-2, reading Code device antenna 1-9, first switch power supply 1-15, second switch power supply 1-16, upset motor 2-3, magnetic core motor 2-9, first are led to It is one-level alarm failure (blinking red lamp) to believe line terminals 1-30, the second communications line terminal 2-1, and whole control system shuts down, The operation of any Cell culture procedures can not be carried out, it is necessary to which Cell culture procedures could be run after fixing a breakdown.Warning lamp 1-3, Radiator fan 1-20, peristaltic pump 1-27, Photoelectric Detection receiver 2-15, Photoelectric Detection transmitter 2-24 are secondary alarm (amber light Flicker), then turning device shuts down, and Cell culture procedures suspend, it is necessary to can just continue to run with cell culture after fixing a breakdown Program.After the System self-test of control system completes not having fault alarm, call in and run by display screen 1-2 and set Cell culture procedures.Turnover bracket 2-11 on turning device is to be in horizontal equilibrium shape when control system is powered and started State, as shown in Fig. 6 A.Main controller controls upset motor starts to rotate clockwise 90 ° to start bit with 60r/min rotating speed Put, as shown in Fig. 6 B.In the process, because the carrier magnetic bead 3-3 of use proportion is smaller than culture base density and contains magnetic conduction Property, carrier magnetic bead 3-3 starts to float up to container framework 3-1 top in the presence of buoyancy.And after 5s time of repose The loop start state of Cell culture procedures is initially entered, as shown in Figure 6 C.After the time of repose by 5s, the magnetic of upper end Core motor is with 40r/min 180 ° of rotating speed rotate counterclockwise, by magnetic core 2-16 rotations on magnetic core support 2-17 towards being deposited in appearance The carrier magnetic bead 3-3 on device framework 3-1 tops, so that it all be adsorbed on container framework 3-1 top, as shown in Figure 6 D.Through Cross after 2s time of repose, upset motor starts 180 ° of rotate counterclockwise as illustrated in fig. 6e with 30r/min rotating speed, by original absorption simultaneously The carrier magnetic bead 3-3 for being deposited in container framework 3-1 tops rotates to bottom, as fig 6 f illustrates.After rotation stops 3s, main frame The peristaltic pump on the case leading section left side start with 5mL/min speed from culture medium storage bag toward container framework 3-1 in bottom fill Note culture medium.While perfusion, another peristaltic pump on the right also begins to the synchronous speed with 5mL/min from container framework 3- Extract out and culture medium waste liquid is crossed by cell consumption in 1 top.After 1min perfusion fresh culture and discharge culture medium, Two peristaltic pumps are synchronously stopped.After perfusion fresh culture every time, master controller all can be according in Cell culture procedures Peristaltic pump operational factor and combine program operation initially through code reader antenna from RF tag above culture medium storage bag (RFID) capacitance values that obtained capacitance values calculate remaining culture medium are read on, and are write again by code reader antenna On RF tag (RFID) above culture medium storage bag, store for read-write next time.Stood by the cell of 6 hours After incubation time, the magnetic core motor of bottom is rotated magnetic core barricade 2-22 with 40r/min 180 ° of rotating speed rotate counterclockwise Towards carrier magnetic bead 3-3, so that carrier magnetic bead 3-3 is under the influence of no magnetic force, started slowly according to the buoyancy of itself Float upwards, and soft agitation is produced in the inside of medium liquid, it is possible thereby to stir the cell cultivated in culture medium, make Cell can be evenly distributed in medium liquid, as shown in Figure 6 G.Then 10s time of repose is passed through so that carrier magnetic Pearl 3-3 all floats to container framework 3-1 top.The magnetic core motor of upper end is revolved counterclockwise with 40r/min rotating speed again Turn 180 °, will by magnetic core 2-16 rotations on magnetic core support 2-17 towards the carrier magnetic bead 3-3 for being deposited in container framework 3-1 tops It is all adsorbed on container framework 3-1 top, as shown in figure 6h.After 2s time of repose, upset servomotor is with 30r/ Min rotating speed starts to turn clockwise 180 ° as shown in fig. 6i, by the former carrier magnetic for adsorbing and being deposited in container framework 3-1 tops Pearl 3-3 rotates to bottom, as shown in Fig. 6 J.Stop in rotation and after the cell quiescent culture 3s times, the magnetic core electricity of bottom Machine is with 40 r/min 180 ° of rotating speed rotate counterclockwise, by magnetic core barricade 2-22 rotations towards carrier magnetic bead 3-3, so that carrying Body magnetic bead 3-3 starts slow floating, and stirring in culture medium upwards under the influence of no magnetic force according to the buoyancy of itself The cell of culture, makes cell be evenly distributed in medium liquid, as shown in fig. 6k.Thus place initially enters a cell culture Circulation pattern, repeat from Fig. 6 C to Fig. 6 K cell cultivation process, this process is a cycle period of cell culture, with this Realize the cycling deposition of cell cycle.After continuously 20 cell culture cycle periods of operation, upset motor is with 60r/min's Rotating speed starts to rotate counterclockwise 90 ° to original position, and as shown in Fig. 6 L, whole Cell culture procedures terminate.

Embodiment two：

ZYX bioreactors S20 of the present utility model is mainly made up of three module sections, as shown in Figure 8.Including control System I, turning device II and cell culture container III etc. are constituted.

As shown in figure 9, in the controls, comprising：Silica gel catheter I -1, mainframe box protecgulum I -2, hinge I -3, protecgulum are saturating Depending on plate I -4, display screen I -5, host switch I -6, mainframe box I -7, host installation plate I -8, main frame I -9, main frame fixed plate I - 10th, mainframe box bonnet I -11, radiator fan I -12, cabinet lock I -13, first switch power supply I -14, second switch power supply I - 15th, alarm I -16, master controller I -17, junction block I -18, power supply installing plate I -19, perfusion drive module I -20, module peace Fill plate I -21, syringe I -22, protection switch I -23, mainframe box power switch I -24, the communication line end of power interface I -25, first Son I -26, beam I -27, signal wire I -28.In overall assembling process, first four beams I -27 are existed with screw lock The bottom of mainframe box I -7；Four hinges I -3 are fixed on to the right and the rear end face of the front end face of mainframe box I -7 two-by-two respectively with screw The corresponding position of mounting hole in the left side on；Protecgulum perspective board I -4 is fixed on mainframe box protecgulum I -2；It will assemble again Mainframe box protecgulum I -2 got up by hinge I -3 with the assembly chaining of mainframe box I -7；By display screen I -5 be arranged on mainframe box I - It is fixed in the window of 7 front end face upper ends；Host switch I -6 is fixed in the hole on the front end face of mainframe box I -7；Main frame is pacified Dress plate I -8 is screwed in the upper end of the inside of mainframe box I -7；Main frame I -9 is placed on host installation plate I -8 again, is used in combination Main frame fixed plate I -10 and screw fix locking；By protection switch I -23, mainframe box power switch I -24 and power interface I -25 It is respectively mounted and is fixed in the Lower mounting hole of the rear end face of mainframe box I -7；By the first communications line terminal I -26 and alarm I -16 In the corresponding hole that the side of mainframe box I -7 is separately fixed at screw；Four syringes I -22 are stuck in module in turn to install On plate I -21, then by perfusion drive module I -20 be fixed by screws on module mounting plate I -21, and with syringe I -22 adjust Linkage has been tried, then the module mounting plate I -21 assembled has been screwed in mainframe box I -7；By first switch power supply I- 14th, second switch power supply I -15, master controller I -17 and junction block I -18 are respectively mounted pair for being arranged in power supply installing plate I -19 Answer on mounting hole, then the power supply installing plate I -19 assembled is screwed in mainframe box I -7；By cabinet lock I -13 and Radiator fan I -12 is respectively and fixedly installed on mainframe box bonnet I -11, then the mainframe box bonnet I -11 assembled is passed through into hinge Chain I -3 is linked at the rear end of mainframe box I -7；Finally silica gel catheter I -1 is respectively fitted on syringe I -22；Again by signal wire I -28 It is connected on the first communications line terminal I -26.One complete control system is assembled into this.

As shown in Figure 10, in turning device, comprising：Motor cover II -1, upset motor II -2, motor support plate II -3, Motor shaft sleeve II -4, turnover bracket plate II -5, pedestal II -6, turnover bracket cover plate II -7, Photoelectric Detection support II -8, photoelectricity inspection Survey receiver II -9, Photoelectric Detection bracket cover II -10, electromagnet magnetic core II -11, silica gel pad II -12, magnetic core cover II -13, photoelectricity Detect transmitter II -14, the second communications line terminal II -15.In total system assembling process, motor support plate II -3 is used into spiral shell Nail is fixed on pedestal II -6；The axle of upset motor II -2 is passed through the axis hole on motor support plate II -3, be screwed lock Tightly；Two electromagnet magnetic cores II -11 are packed into two magnetic core covers II -13 respectively, then use spiral shell after putting silica gel pad II -12 respectively Nail fixation is locked at the two ends of turnover bracket cover plate II -7；By II -14 point of Photoelectric Detection receiver II -9 and Photoelectric Detection transmitter It is not embedded in into two Photoelectric Detection supports II -8, then two Photoelectric Detection bracket covers II -10 are buckled fixation respectively；By two It is separately fixed at the electro-detection support II -8 installed with screw above and below the front end face of turnover bracket cover plate II -7, contains electro-detection Receiver II -9 is fixed on upper end, containing Photoelectric Detection transmitter II -14 is fixed on lower end；Again by turnover bracket plate II- The 5 turnover bracket cover plate II -7 with assembling is screwed locking；By the turnover bracket plate II -5 and motor shaft sleeve that assemble II -4 is fixed on screw lock above the rotating shaft of upset motor II -2；Second communications line terminal II -15 is fastened on screw In mounting hole in motor cover II -1, and connect corresponding power line is corresponding with data wire, finally again by motor cover II -1 Cover upset motor II -2 to be screwed on motor support plate II -3, and screw positioning lock is used in bottom base II -6 Tightly.One complete turning device is assembled into this.

As shown in figure 11, in cell culture container, comprising container framework III -1, end cover III -2, bead inlet port end cap III - 3rd, carrier magnetic bead III -4.A number of carrier magnetic bead III -4 is put into container framework from the bead inlet port in container framework III -1 III -1 inside space in, then by bead inlet port end cap III -3 be tightened on the bead inlet port of container framework III -1 seal, then will sealing The screwing hermetic of end cap III -2 is assembled into a complete cell culture container in container framework III -1 with this.

As shown in fig. 7, the silica gel catheter I -1 drawn in above control system passes through the groove of the front end of mainframe box protecgulum I -2 Mouthful, it is respectively connected in container framework III -1 on corresponding interface, it is ensured that the silica gel catheter I -1 picked out from syringe I -22 is connected to Input in container framework III -1, and another silica gel catheter I -1 is connected to the outlet side in container framework III -1, then Signal wire I -28 is linked on the interface of the second communications line terminal II -15 in motor cover II -1, then cell culture container III is filled in Enter on turning device II in the upper neck of magnetic core cover II -13, thus constitute a complete ZYX bioreactors S20.

ZYX bioreactors S20 workflow is as follows：

Referring to Fig. 9,10,11, according to the program woven in control system, command information is converted into by master controller, Turning device is controlled to be acted by command information.After control system is powered and started, it is necessary to logical before the Cell culture procedures that bring into operation Whether the operation for initial detecting each electric system of master controller I -17 crossed in control system is normal.Comprising display screen I -5, First switch power supply I -14, second switch power supply I -15, alarm I -16, master controller I -17, the first communications line terminal I -26, Upset motor II -2, Photoelectric Detection receiver II -9, electromagnet magnetic core II -11, Photoelectric Detection transmitter II -14, the second order wire The grade electrical equipment of terminal II -15.If a certain part therein is problematic, runs abnormal or do not act, then can send report Warn, and the buzzer on alarm I -16 sounds the alarm.Wherein first switch power supply I -14, second switch power supply I -15, Alarm I -16, master controller I -17, perfusion drive module I -20, the first communications line terminal I -26, upset motor II -2, electromagnetism Magnetic core II -11, the second communications line terminal II -15 are one-level alarm failure (alarm song is lasting), and control system shuts down, it is impossible to The operation of any Cell culture procedures is carried out, it is necessary to which Cell culture procedures could be run after fixing a breakdown.Radiator fan I -12, light Electro-detection receiver II -9, Photoelectric Detection transmitter II -14, be secondary alarm failure (alarm song interval), then turning device stops Only operate, Cell culture procedures suspend, it is necessary to can just continue to run with Cell culture procedures after fixing a breakdown.In control system self-inspection Complete not having after fault alarm, called in by display screen I -5 and run the Cell culture procedures set.On turning device Turnover bracket plate II -5 control system be powered start when be in horizontal equilibrium state, as illustrated in fig. 12.Master controller control Upset motor processed starts to rotate clockwise 90 ° to original position with 40r/min rotating speed, as shown in Figure 12 B.In the process, Because the proportion of the carrier magnetic bead III -4 of use is bigger than culture base density and with magnetic conductivity, carrier magnetic bead III -4 starts in gravity In the presence of sink down into the bottom of container framework III -1.And following for Cell culture procedures is initially entered after 4s time of repose Ring starting state, as indicated in fig. 12 c.After rotation stops 4s, the perfusion drive module inside mainframe box starts with 1mL/min Speed from first in four syringes toward in container framework III -1 bottom irrigate culture medium.While perfusion, four The 3rd in individual syringe also begins to the synchronous speed with 1mL/min and is extracted out from the top of container framework III -1 by cell Consumed culture medium waste liquid.After 1min perfusion fresh culture and discharge culture medium, perfusion drive module is stopped. After the cell quiescent culture time of 2 hours, the electromagnet magnetic core II -11 of bottom is produced by main controller controls energization Magnetic force, so that carrier magnetic bead III -4 all be adsorbed in the bottom of container framework III -1, as indicated in fig. 12d.Upset motor with 40r/min rotating speed starts 180 ° of rotate counterclockwise, as shown in figure 12e, and original is adsorbed and the bottom of container framework III -1 is deposited in Carrier magnetic bead III -4 go to upper end, as shown in Figure 12 F.The electromagnet magnetic core II -11 of upper end is powered off by main controller controls, Magnetic force is disappeared, so that carrier magnetic bead III -4 is under the influence of no magnetic force, slow sinking is started according to the gravity of itself, and Soft agitation is produced in the inside of medium liquid, the cell cultivated in culture medium is stirred with this, cell is uniformly divided Cloth is in medium liquid, as shown in fig. 12g.Then 12s time of repose is passed through so that carrier magnetic bead III -4 all sinks simultaneously The static bottom to container framework III -1.It is powered again by the electromagnet magnetic core II -11 of bottom by main controller controls and produces magnetic force, By the carrier magnetic bead III -4 of bottom, all absorption is in the bottom of container framework III -1, as shown in Figure 12 H.Upset motor start with 40r/min rotating speed starts to turn clockwise 180 °, as shown in figure 12i, and original is adsorbed and the bottom of container framework III -1 is deposited in Carrier magnetic bead III -4 rotate to top, as shown in Figure 12 J.The electromagnet magnetic core II -11 of upper end is powered off by main controller controls Magnetic force is disappeared, so that carrier magnetic bead III -4 is under the influence of no magnetic force, slow sinking is started according to the gravity of itself, and The cell cultivated in agitation culture medium, makes cell be evenly distributed in culture medium, as shown in Figure 12 K.And thus place start into Enter the circulation pattern of a cell culture, repeat the cell cultivation process from Figure 12 C to Figure 12 K, this process is cell culture One cycle period, the cycling deposition of cell cycle is realized with this.After continuously 20 cell culture cycle periods of operation, turn over Rotating motor starts to rotate counterclockwise 90 ° to original position with 40r/min rotating speed, as shown in Figure 12 L, whole cell culture journey Sequence terminates.

In described in two above example, during whole Cell culture procedures operation, all electrical equipment components are all In real-time monitoring state, if you have questions, then corresponding fault alarm occurs.

The embodiment of above-described two cell culture, is that, in order to be further illustrated to of the present utility model, should not manage Solve that the content included by the utility model is completely covered.And be not used in limitation and cultivate the species of cell, and for The rotating speeds of all motors defined in the present embodiment, the rotation direction of motor, the rotational angle of motor, each flow definition The limitation of the parameter settings such as the content volume of time and culture medium perfusion.

In embodiment one, magnetic core uses powerful permanent magnetic iron, and its magnetic force size and Orientation is all fixed.Can only be by Magnetic core motor rotates the absorption and release to realize magnetic force of magnetic core and magnetic core barricade.In embodiment two, electromagnet magnetic core is Variable magnetic field is created using silicon steel sheet and enamel covered wire.Magnetic field is adjusted by converting electric current, voltage and sense of current Intensity and magnetic line of force direction.The presence or absence of magnetic force can be controlled by the break-make of electric current；The size of magnetic force can use the strong of electric current How much weak or enamel covered wire the number of turn controls；Also it can be controlled by changing resistance control electric current size；The magnetic pole of electromagnetism can To be controlled by change sense of current.Selection line footpath 0.12mm enamel-covered wire is wrapped in a diameter of 50mm circular iron core electromagnetism Electromagnet magnetic core is made on iron, in 48v operating at voltages, magnetic force is 1.05kg, plus 0.1s durations backward voltage, 4x20cm is in 4- 8V, 1x3cm more than 90% can freely be fallen under 2-4V.Selection line footpath is wrapped in E type Z10 silicon steel for 0.21mm enamel-covered wire On piece so that size is the long 57mm high 37mm of wide 47mm, it is 2.5kg that silicon steel sheet is added in the magnetic force surveyed under 5.5v voltages；It is disconnected When electric, electromagnet magnetic core demagnetization, 4x20cm silicon steel sheets can freely be fallen, plus 0.1s durations backward voltage, 1x3cm is under 2-4V More than 90% can freely fall.Under normal "on" position, electromagnet magnetic core can generate heat generation high temperature, and can be passed to culture vessel Lead, in the environment of 37 DEG C of incubator, electromagnet 37 ± 1 DEG C of permissible value of heating, in the case where meeting temperature conditionss, can be used any Electromagnet magnetic core.

When in cell cultivation process, turning device and cell culture container are put into CO₂In incubator, in incubator Temperature and humidity can produce corrosiveness, outside turning device for closing system.The core of turning device is upset electricity Machine, upset motor connects flip-arm by motor shaft sleeve, so as to control whole flip-arm, flip-arm is fitted with cell culture appearance Device, magnetic core is equipped with the two ends of flip-arm.Flip-arm avoids the mistake because of flip-arm by controlling the anglec of rotation of upset motor Degree rotation causes the circuit in machine to be wrapped in centre.

Carrier magnetic bead in embodiment one, two is using material conducts such as polyethylene, polypropylene, makrolon, polystyrene Magnetizable ferroso-ferric oxide or silicon steel sheet are wrapped up in shell material, the inside, and the diameter of carrier magnetic bead is more than 1 millimeter, held less than culture Side most short in device., it is necessary to carry out sterilization treatment to carrier magnetic bead when cultivating suspension cell；When cultivating attached cell, to carrier Magnetic bead sterilizes again after carrying out adherent processing, and adherent processing here includes TC processing, albumen coating, poly glycolyx, plasma Processing etc..

Culture vessel in embodiment one, two：Culture vessel is using polyethylene, polypropylene, makrolon, polystyrene etc. Square frame is made in material, and the container of confined space is made in both sides breathable Teflon film covering up and down.For ease of thin Nutrients when born of the same parents cultivate are exchanged, substituted, and the both sides up and down of culture vessel are respectively provided with the port of two and external connection, are such as schemed Shown in 13.Side is available for the port (D, H interface on such as Figure 13) that carrier comes in and goes out provided with one, and size is slightly more straight than carrier magnetic bead Footpath is big, another relative provided with three gateways (A, B, C, E, F, G interface on such as Figure 13) exchanged for nutrient solution, is distributed in frame Surrounding, is provided with one import of one outlet, and outlet is supplied into fresh for the output of the spent media consumed, import Nutrient solution, two mouths can work simultaneously, individually can also be come in and gone out as needed for nutrient solution.The Interface design of culture vessel is The shape (A on such as Figure 13) that can be connected with for female Luer or the taper (E on such as Figure 13) being connected with silica gel catheter.Training The overall container that Teflon film and frame are combined into container is supported, the mode for using neck is agreed with, then is entered in outer layer Row is fixed, and glue can be added it is more sealed.Polystyrene frame interior smooth, radian is designed with the place of each corner Corner.

The working condition of embodiment one, two：Carrier magnetic bead is added in cell culture container, carrier magnetic bead can not only be used for adherent The carrier of cell culture, while also serving as stirring tool.

Cultivate attached cell：Attached cell is the surface for sticking to carrier magnetic bead or culture vessel.Cell culture container is protected Hold and slowly stir 24 hours, allow cell adherence in carrier magnetic bead surfaces.At second day, unfinished adherent cell will be included Culture medium is discharged into waste material container or syringe, then adds fresh culture.Cell is adsorbed in carrier magnetic bead surfaces, with load Up and down motion of the body magnetic bead in culture vessel and the purpose for reaching stirring.By set the slewing rate of flip-arm, direction with And the size of magnetic field magnetic force, in cell culture, open the magnetic field of culture vessel bottom, by the carrier magnetic bead control containing cell System in the upper end or lower end of culture vessel, make cytotostatic motionless, culture a period of time after remove magnetic field so that carrier magnetic bead by Buoyancy or Action of Gravity Field and produce and rise or sink down into the two ends of culture vessel, then open two ends magnetic field, make carrier magnetic bead stable At the two ends of culture vessel, culture vessel is then rotated, cell and carrier magnetic bead is rotated to the other end of culture vessel, is continued Cyclic culture, until culture terminates.

Cultivate suspension cell：Suspension cell is suspended in nutrient solution.During culture, cell is put into containing carrier magnetic bead Culture vessel.Culture vessel is attached on the flip-arm of bioreactor, Primary culture program, bioreactor elder generation automatic sorting Cell, is then injected into culture medium, starts culture.Before culture and during culture, host computer analyzes cell according to photoelectric detector The data record and the growth index of reporter cell that density is obtained are there is provided supplementing culture medium, change culture medium or stop culture It is recommended that.Cultivate in cell processes, according to program and the increased result of cell density, carrier magnetic bead is according to the shape of program and microenvironment State, suspension is realized by the change in magnetic field or is adsorbed, to reach the purpose of stirring.After the completion of cell culture, it can harvest complete Portion's cell, or cell is imported into the function that another container continues culture, realization classification amplification or amplification.

Instrument distribution situation in embodiment one, two：Whole bioreactor is trained by control system, turning device and cell Support container composition.Control system mainly by connection and turning device connecting communication and is controlled, and passes through silica gel catheter and cell Culture vessel connection imports fresh culture or export culture medium waste liquid.In cell cultivation process, cell culture container is peace Dress is fixed on turning device, drives cell culture system together to rotate by the flip-arm on turning device.Meanwhile, in cell Turning device and cell culture container can be together placed on CO in incubation₂Worked in incubator, CO₂Incubator is thin Intracellular growth provides suitable CO₂Concentration, temperature and humidity, as shown in figure 14.α in shown in Figure 14 is CO₂Incubator, β and γ points Turning device that Wei be in embodiment one and embodiment two.

Pass through above example, CO₂The equipment and instrument that incubator can be integrated into one with bioreactor is thin to realize Born of the same parents cultivate, and design a closed cavity in inside bioreactor, it includes or contains all CO₂The function of incubator, can To realize one or more turning devices in bioreactor while being cultivated, and it can be adjusted simultaneously by host computer The CO of bioreactor box house₂Concentration, O₂The condition of culture such as concentration, temperature and humidity.Integrate CO₂Cultivate box function Bioreactor, it is possible to prevente effectively from turning device wiring and conduit pass through CO₂During incubator, CO is destroyed₂During incubator seal The problem of bringing.

Claims (14)

1. a kind of multifunctional bio reactor for being used to cultivate cell and cell sorting, it is characterised in that：Including turning device, carefully Born of the same parents' culture vessel, turning device includes the turnover bracket that can be operated around spatial axes, is sent out with the magnetic field of turnover bracket physical connection Generating apparatus, cell culture container is removably fixed on turnover bracket, cell culture container be located at above-mentioned spatial axes or On its extended line, turnover bracket is followed to be operated according to certain rules with track, cell culture container end face is filled close to magnetic field Put, the controllable change around cell culture container periphery and internal magnetic field of field generator for magnetic.

2. multifunctional bio reactor according to claim 1, it is characterised in that the turning device includes pedestal, motor Cover, electric machine support, upset motor, turnover bracket, magnetic core, seal gasket；Upset motor is fixed on electric machine support, motor cover cover In upset motor and push down seal gasket and be fixed on electric machine support；Magnetic core is arranged in turnover bracket；Turnover bracket is enclosed on It is fixed in upset motor with electric machine support on pedestal；Described upset motor can be set according to program parameter to be turned by certain Speed, frequency and positive and negative two rotate to angle.

3. multifunctional bio reactor according to claim 1, it is characterised in that described cell culture container includes holding Device framework, carrier；Carrier is positioned over inside container framework by the end interface of container framework.

4. multifunctional bio reactor according to claim 1, it is characterised in that also comprising control system, by mainframe box, Display screen, master controller, Switching Power Supply, cabinet cover plate composition, be responsible for the interface display of reactor, operation, program operation with The processing of the human-machine interactive information of setting, and the program set from master controller or command information are converted into e-mail Number, and then manipulate the work of electric component in whole bioreactor, and by the feedback of the information obtained from controlled electric component to Master controller is simultaneously shown on the display screen.

5. multifunctional bio reactor according to claim 1, it is characterised in that the turnover bracket of described turning device On be equipped with one or more cell culture containers.

6. multifunctional bio reactor according to claim 2, it is characterised in that the motion mode of described turnover bracket To overturn, waving, parabolic motion, curvilinear motion or move along a straight line up and down.

7. multifunctional bio reactor according to claim 2, it is characterised in that described turnover bracket two side ends are installed There is magnetic core, magnetic core is made up of electromagnet or permanent magnet；When magnetic core uses permanent magnet, cooperation is equipped with magnetic core support and shielding Magnetic sheet, is arranged on magnetic core support two sides by shielding magnetic sheet and magnetic core, the presence or absence of magnetic force is realized by the rotation of magnetic core support； When magnetic core use electromagnet, the size of magnetic force, direction are controlled by the size of control electric current, direction and break-make and is whether there is, The function of absorption or release vehicle is reached with this, so as to realize different existences of the carrier in container framework.

8. multifunctional bio reactor according to claim 7, it is characterised in that described magnetic core and container framework two ends The distance in face is no more than 200 millimeters.

9. multifunctional bio reactor according to claim 2, it is characterised in that the flip angle of the upset motor is suitable Hour hands rotation is no more than 360 °, and rotate counterclockwise is no more than 360 °.

10. multifunctional bio reactor according to claim 2, it is characterised in that be equipped with light on described turnover bracket Photodetector, it includes photo resistance and illuminator and various photoelectric detection probes.

11. multifunctional bio reactor according to claim 10, it is characterised in that described photoelectric detector passes through Electrode contact pin mode is plugged on turnover bracket；Or snapped onto by neck snap on turnover bracket；Or it is solid by welding It is scheduled on turnover bracket；Or be fixed on by latch on turnover bracket；Or be screwed on turnover bracket；Or pass through magnetic Absorption can realize conductive, transmission data function on turnover bracket by its interface.

12. multifunctional bio reactor according to claim 4, it is characterised in that integrated built-in CO in described mainframe box₂ Box function is cultivated, by turning device and cell culture container integrated installation in built-in CO₂In incubator.

13. multifunctional bio reactor according to claim 4, it is characterised in that perfusion is provided with described mainframe box Pump, the course of work of charge pump is main controller manipulation.

14. multifunctional bio reactor according to claim 4, it is characterised in that can be equipped with and hang on described mainframe box Bar support.