CN206177629U - A device for plant roots secretion is collected - Google Patents
A device for plant roots secretion is collected Download PDFInfo
- Publication number
- CN206177629U CN206177629U CN201621207458.5U CN201621207458U CN206177629U CN 206177629 U CN206177629 U CN 206177629U CN 201621207458 U CN201621207458 U CN 201621207458U CN 206177629 U CN206177629 U CN 206177629U
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- CN
- China
- Prior art keywords
- plant
- culture tube
- root
- root exudates
- exudates
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The utility model relates to a device for plant roots secretion is collected belongs to plant roots secretion collection technique field. The device includes: quartz glass cotton (2) of culture tube (1) are placed in culture tube (1), place breather valve (3) that there are rubber packing ring (4) culture tube (1) below, its overhead in, receiving flask (5) be connected through dull polish mouth and culture tube (1). The material that the device adopted the municipality to purchase is makeed according to conventional method. Beneficial effect lies in: simple structure, with low costs, convenient operation, plant root system growing environment has been simulated to the the device at utmost, reduces the influence of soil microorganism to root exudates to can avoid nutrient solution and root exudates's interact, the device kneck is the dull polish mouth, and sealing performance is good, has avoided volatile materials to volatilize, the receiving flask can take off at any time, can go on regularly or the continuity collection root exudates, and the plant need not not be extracted to whole collection process, guarantees the integrality in the plant root system growth course.
Description
Technical field
The utility model is related to a kind of device collected for secretions from plant roots, and platymiscium root exudates collects skill
Art field.
Background technology
Plant in growth course by root system to rhizosphere soil secrete many kinds of substance, i.e. root exudates, including phenolic acid,
Amino acid, low molecular weight organic acid, soluble sugar, soluble protein, mucilage, cast etc..Root exudates is to adjust
Rhizosphere microecological system vigor and the key factor of function, form and Nutrient Activation, plant material circulation nutrient in soil texture
Absorb, environment remediation is coerced the aspects such as alleviation, signal transduction allelopathy and all has important function, is plant-soil-microorganism
Conditioning agent between three.In the last few years, the importance of root exudates caused the highest attention of domestic and international researcher.But
Be, due to the complexity of plant strain growth environment, soil it is various for the absorption and utilization of root exudates, root exudates composition
The features such as property, causes root exudates to be difficult to correct, efficient collection and extraction.
At present, the collection of root exudates and extracting method are various, including (1) solution train collecting method, will plant be put into
The collection of root exudates is carried out after cultivating in solution.The collecting method utilizes Liquid Culture, can make many plant growths in anaerobism
In environment, cause root exudates exception, in addition the method can not provide aseptic condition, microbes are produced to root exudates
Affect, and nutrient solution contains different kinds of ions, has an effect with root exudates, its filtration step is loaded down with trivial details, complexity, there is also certain
Rotten, root-rot phenomenon；(2) earth culture collecting method, concrete operation step be the rhizosphere soil of plant is taken out mix with sterilized water,
Vibration, centrifugation are filtered, and gained filtrate is root exudates, and it is through adsorption by soil and micro- that the method collects the material for obtaining
Material after bioconversion degraded, it is impossible to reflect the truth of root exudates；(3) root system elution method, will plant fix
The growth in soil takes out plant after a period of time, and to root system drip washing repeatedly, gained is exactly root exudates.The method is taken out and is planted
Is difficult to avoid that during strain and root system is caused certain damage, the inclusion containing plant and bleeding sap, cause root system in root exudates
The uncertainty of secretion composition；(4) other collection methods.The design of also such as continuity collection system, such as Germany scientist
The Trace bio-element cup of collecting root exudate in situ, Australian Scientists devise extraction ring to collect water planting Lupinus albus
Root exudates, had annular hollow fibrosampler, bottom to contain devices such as the root case of nylon solarium etc. later.These dresses
Install meter complicated, whole process time of collecting is longer, easily causes the pollution of whole system, the amount also bad control of nutrient solution.
In sum, the collection device of current root exudates cuts both ways, also without one kind can either simulating plant just
It is frequently grown, microorganism pollution can be avoided again and a kind of device that root exudates is truly collected is realized.
The utility model aims to provide a kind of more economic, convenient operation root exudates collection device, is root system
The research of secretion provides facility.
The content of the invention
The purpose of this utility model is to overcome the shortcomings of existing root exudates collection device, and provides a kind of simple
The easy device collected for secretions from plant roots.
The device collected for secretions from plant roots of the present utility model includes：Culture tube (1), is placed in culture tube (1)
In quartz glass cotton (2), be placed in culture tube (1) lower section, the breather valve (3) of rubber washer (4) be equipped with thereon, by frosted mouth
The receiving flask (5) being connected with culture tube (1).
The utility model is made according to a conventional method using commercial material.
The beneficial effects of the utility model are：
1st, simple structure, low cost, easy to operate；
2nd, the device at utmost simulates plant root growing environment, reduces shadow of the edaphon to root exudates
Ring, and the interaction of nutrient solution and root exudates can be avoided.
3rd, with masking foil parcel periphery with lucifuge, it is to avoid moss growth effect plant root grows, and pollutes root exudates；
The unlatching of breather valve is keeping the hydrophobicity and gas permeability of plant root, it is to avoid rotten of plant, root-rot phenomenon.
4th, frosted mouth, good seal performance are at device interface, it is to avoid volatile substances.
5th, receiving flask can be removed at any time, periodically or continuously property can be carried out to root exudates and is collected, it is whole collect process without
Plant must be extracted, it is ensured that the integrality in plant root growth course.
Description of the drawings
Fig. 1 is structural representation of the present utility model.
Specific embodiment
The utility model is described in further detail below in conjunction with the accompanying drawings.
As shown in drawings, the utility model patent includes for the device that secretions from plant roots is collected：Culture tube 1, puts
Quartz glass cotton 2 in culture tube 1, is placed in the lower section of culture tube 1, the breather valve 3 of rubber washer 4 is equipped with thereon, by frosted
The receiving flask 5 that mouth is connected with culture tube 1.
Described culture tube 1 is made using the glass tube of diameter 43mm, long 160mm, and bottom outer end is frosted mouth, to increase
Device seal；The bottom of culture tube 1 is placed with silica wool 2, and then adding the plant cultivation media of sterilizing is used to cultivate plant.Stone
The effect of English cotton 2 is to prevent to be leaked under cultivation matrix.Culture tube 1 carries out lucifuge process, mould using masking foil parcel during collection
Intend the dark state of root growth.
Described breather valve 3 makes of polytetrafluoroethylene (PTFE), detachable convenient cleaning, long 50mm, diameter 20mm, and rubber is used on both sides
Rubber gasket 4 seals water-tight, it is ensured that the sealing of device, rubber washer 4 also can be dismantled.The top of breather valve 3 is direct with culture tube 1
It is connected, bottom is connected with a glass round tube.Glass round tube first half ampuliform, high 45mm, lower half is frosted interface, high
35mm。
Described receiving flask 5 is made using glass, and capacity is 250mL, and bottom of bottle width 90mm, bottle height 120mm, bottleneck adopts mill
Sand formula, the high 25mm of bottleneck.It is connected with culture tube 1 by frosted mouth.
Before root exudates is collected, will repeatedly clean after quartz sand salt acid soak 48h to PH=7 or so, it is dry in 160 DEG C
It is stand-by normal temperature to be down to after heat sterilization 12h；A small amount of quartz glass cotton is put in the bottom of culture tube 1, by plant plant or transplant in
In culture tube 1, plant root is kept to unfold, it is peripheral with masking foil parcel culture tube 1, to keep the dark bar of plant root growth
Part, it is to avoid moss grows；Breather valve 3 is kept to open during plant strain growth, to guarantee that device is ventilated, it is to avoid root system of plant anaerobism
Growth.The plant strain growth phase adds nutrient solution into culture tube to guarantee normal plants, adds before root exudates is collected and steams
Distilled water will manage interior nutrient solution and rinse well, and relief plant stands growth 24h.Quantitative distilled water is subsequently adding, root system is secreted
Thing is eluted.Aperture of the root exudates of wash-out in the middle of breather valve 3 is oozed out into 5 in receiving flask, completes the receipts of root exudates
Collection.
Practical application shows that this device fully meets design requirement.
Claims (1)
1. it is a kind of for secretions from plant roots collect device, it is characterised in that the device includes：Culture tube (1), is placed in training
The quartz glass cotton (2) in pipe (1) is supported, culture tube (1) lower section is placed in, is equipped with the breather valve (3) of rubber washer (4) thereon, led to
Cross the receiving flask (5) that frosted mouth is connected with culture tube (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621207458.5U CN206177629U (en) | 2016-11-09 | 2016-11-09 | A device for plant roots secretion is collected |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621207458.5U CN206177629U (en) | 2016-11-09 | 2016-11-09 | A device for plant roots secretion is collected |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206177629U true CN206177629U (en) | 2017-05-17 |
Family
ID=58684074
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621207458.5U Expired - Fee Related CN206177629U (en) | 2016-11-09 | 2016-11-09 | A device for plant roots secretion is collected |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206177629U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109406244A (en) * | 2018-12-29 | 2019-03-01 | 中国科学院南京地理与湖泊研究所 | A kind of water plant root exudates original position collection device |
| CN113945427A (en) * | 2021-10-18 | 2022-01-18 | 东北林业大学 | Actinidia arguta bleeding sap collection device |
-
2016
- 2016-11-09 CN CN201621207458.5U patent/CN206177629U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109406244A (en) * | 2018-12-29 | 2019-03-01 | 中国科学院南京地理与湖泊研究所 | A kind of water plant root exudates original position collection device |
| CN109406244B (en) * | 2018-12-29 | 2023-10-10 | 中国科学院南京地理与湖泊研究所 | Aquatic plant root system secretion normal position collection device |
| CN113945427A (en) * | 2021-10-18 | 2022-01-18 | 东北林业大学 | Actinidia arguta bleeding sap collection device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170517 Termination date: 20191109 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |