CN207923478U - A kind of seedling root secretion collection device - Google Patents
A kind of seedling root secretion collection device Download PDFInfo
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- CN207923478U CN207923478U CN201721213942.3U CN201721213942U CN207923478U CN 207923478 U CN207923478 U CN 207923478U CN 201721213942 U CN201721213942 U CN 201721213942U CN 207923478 U CN207923478 U CN 207923478U
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- watering
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- resin column
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Abstract
The utility model is related to a kind of seedling root secretion collection devices, including cultivating container（1）And watering can（5）, in watering can（5）Built-in nutrient solution（15）, pass through pipeline and watering can（5）The nozzle of connection（6）It is placed in cultivating container（1）Top, in the cultivating container（1）It is interior to be equipped with five-layer structure, it is followed successively by pebble layer from top to bottom（3）, mineral wool layer（4）, sterilizing coarse sands layer（14）, pebble layer（3）With mineral wool layer（4）, potting nursery stock（2）Root system extend to sterilizing coarse sands layer（14）It is interior, cultivating container（1）Lower section be connected to resin column tube（7）, in resin column tube（7）Interior potting resin（8）, resin column tube（7）End connect downcomer（10）, downcomer（10）Stretch into beaker（11）It is interior.
Description
Technical field
The utility model belongs to nursery stock culture technique field more particularly to a kind of seedling root secretion collection device.
Background technology
Secretions from plant roots be the inorganic ions that root system of plant is discharged during vital movement by rhizospheric environment and
The general designation of organic compound.Secretions from plant roots reflect vegetation growth state direct indicator object, test result plant,
Many research fields such as soil, environment, ecology have important value.But the collection of root exudates is always then research field
Specifically how international problem, can be collected into not by microbial contamination and energy accurate representation plant is divided in habitat conditions
Secretion is more difficult.Existing root exudates collection device and method rest on always the exploratory stage.Root system point in short time
Secretion is less, and utilization of the soil colloid to the absorption and microorganism of root exudates to root exudates, causes in nature in addition
Under the conditions of collect root exudates it is extremely difficult, this seriously constrains the understanding of plant rhizosphere biology ecological processes.
Utility model content
In order to solve the above-mentioned technical problem, the utility model provides a kind of seedling root secretion collection device, this practicality
Novel device uses resin adsorption fine-root secretion, the utility model to avoid microorganism and led to root exudates utilization
The secretion composition transfer of cause；Meanwhile the utility model is to inquire into seedling root under varying environment gradient to secrete species variation
Provide solution.
The utility model is achieved by the following technical solution, and the utility model provides a kind of seedling root secretion receipts
Acquisition means, including cultivating container and watering can, in the built-in nutrient solution of watering can, the nozzle being connect with watering can by pipeline, which is placed in cultivation, to be held
The top of device is equipped with five-layer structure in the cultivating container, is followed successively by pebble layer, mineral wool layer, sterilizing from top to bottom slightly
The root system of layer of sand, pebble layer and mineral wool layer, potting nursery stock extends in sterilizing coarse sands layer, and the lower section of cultivating container is connected to resin
The end of column tube, the potting resin in resin column tube, resin column tube connects downcomer, and downcomer stretches into beaker.
Preferably, the size of potting resin is the cm of 1.5 cm × 4.0 in resin column tube 7.
Preferably, being equipped with pressurizing valve in watering can.For pressurized jet nutrient solution.
Preferably, being equipped with flow-limiting valve on the pipeline that watering can is connect with nozzle.Limitation sprays into the amount of nutrient solution.
Preferably, being clamped equipped with sealing in the end of resin column tube.The beneficial effects of the utility model are：
The utility model avoids microorganism and utilizes caused secretion composition transfer to root exudates；Meanwhile this reality
With novel solution is provided to inquire into seedling root secretion species variation under varying environment gradient.
Description of the drawings
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Or attached drawing needed to be used in the description of the prior art is briefly described, it should be apparent that, the accompanying drawings in the following description is only
It is some embodiments of the utility model, for those of ordinary skill in the art, in the premise not made the creative labor
Under, other drawings may also be obtained based on these drawings.
Fig. 1 is the schematic diagram of the utility model structure；
In figure：1, cultivating container, 2, potting nursery stock, 3, pebble layer, 4, mineral wool layer, 5, watering can, 6, nozzle, 7, resin
Column tube, 8, resin, 9, sealing pincers, 10, downcomer, 11, beaker, 12, sand core, 13, flow-limiting valve, 14, sterilizing coarse sands layer, 15, battalion
Nutrient solution, 16, pressurizing valve.
Specific implementation mode
In order to make the purpose of the utility model, technical solutions and advantages more clearly understood, with reference to embodiments, to this
Utility model is further elaborated.It should be appreciated that specific embodiment described herein is only used to explain this practicality
It is novel, it is not used to limit the utility model.
Below in conjunction with the accompanying drawings and specific embodiment is further described the application principle of the utility model.
Embodiment 1, such as Fig. 1, a kind of seedling root secretion collection device, including cultivating container 1 and watering can 5, in watering can 5
Built-in nutrient solution 15, the nozzle 6 being connect with watering can 5 by pipeline are placed in the top of cultivating container 1, in the cultivating container 1
Equipped with five-layer structure, it is followed successively by pebble layer 3, mineral wool layer 4, sterilizing coarse sands layer 14, pebble layer 3 and mineral wool from top to bottom
The root system of layer 4, potting nursery stock 2 extends in sterilizing coarse sands layer 14, the lower section connection resin column tube 7 of cultivating container 1, in resin column tube
The end of potting resin 8 in 7, resin column tube 7 connects downcomer 10, and downcomer 10 stretches into beaker 11.
The size of potting resin is the cm of 1.5 cm × 4.0 in resin column tube 7.
Pressurizing valve 16 is equipped in watering can 5.
Flow-limiting valve 13 is equipped on the pipeline that watering can 5 is connect with nozzle 6.
It is equipped with sealing in the end of resin column tube 7 and clamps 9.
Using the concrete operation step of resin adsorption fine-root secretion device：
1）Resin pre-processes：A certain amount of Amberlite XAD-16 type resins are weighed, triangular flask is placed in, absolute ethyl alcohol is added to soak
Steeping 24 h makes it fully be swollen；Resin is rinsed with aqua sterilisa to glass funnel afterwards（Add filter paper）, first ethyl alcohol is rinsed with pure water
Totally, no longer muddy to efflux with the acetone rinsing resin of 2BV, then continue to rinse with 3BV absolute ethyl alcohols, then will with pure water
Ethyl alcohol is cleaned；Rinsed with the NaOH of 4BV 2mol/L, after resin is rinsed to neutrality with pure water, then with the hydrochloric acid of 4BV 2mol/L
Rinse, after resin is rushed to neutrality with pure water, resin is placed in 65 DEG C of oven dryings, is sealed spare.
2）The collection of root exudates：The resin that will have been pre-processed weighs 1.0g and is placed in resin column tube, installation to collection dress
In setting, often 5 days circulating collection root exudates of culture are primary, every time 3 cycle, flow velocity 25mlmin-1, after collecting every time,
The nutrient solution re-replaced in Culture basin continues to cultivate to seedling, and Leaves of Poplar Seedlings appearance is withered under nutritional deficiency culture after 18d
Yellow phenomenon, experiment terminate.Root exudates is collected 3 times total.
3）The desorption of phenolic acid in root secretion：It collects and finishes every time, with deionized water by the XAD-16 trees in resin column tube
Fat is all rushed into V-type funnel made of filter paper, then is rinsed repeatedly several times with deionized water, and fully weighing 0.5g after drying is placed in
70% ethyl alcohol stripping liquids of 20ml are added in 100ml triangular flasks, and 25 DEG C of oscillations desorb for 24 hours, Aspirate supernatant after standing, through 0.22um
Membrane filtration carries out chromatography.
The utility model avoids microorganism and utilizes caused secretion composition transfer to root exudates；Meanwhile this reality
With novel solution is provided to inquire into seedling root secretion species variation under varying environment gradient.
Certainly, above description is also not limited to the example above, and technical characteristic of the utility model without description can lead to
It crosses or realizes that details are not described herein using the prior art；Above example and attached drawing are merely to illustrate the technology of the utility model
Scheme is not limitations of the present invention, and the utility model is described in detail with reference to preferred embodiment, this
Field it is to be appreciated by one skilled in the art that those skilled in the art institute in the essential scope of the utility model
The variations, modifications, additions or substitutions made without departure from the objective of the utility model, want by the right that should also belong to the utility model
Seek protection domain.
Claims (5)
1. a kind of seedling root secretion collection device, it is characterised in that：Including cultivating container（1）And watering can（5）, in watering can
（5）Built-in nutrient solution（15）, pass through pipeline and watering can（5）The nozzle of connection（6）It is placed in cultivating container（1）Top, described
Cultivating container（1）It is interior to be equipped with five-layer structure, it is followed successively by pebble layer from top to bottom（3）, mineral wool layer（4）, sterilizing coarse sands layer
（14）, pebble layer（3）With mineral wool layer（4）, potting nursery stock（2）Root system extend to sterilizing coarse sands layer（14）It is interior, cultivating container
（1）Lower section be connected to resin column tube（7）, in resin column tube（7）Interior potting resin（8）, resin column tube（7）The lower water of end connection
Pipe（10）, downcomer（10）Stretch into beaker（11）It is interior.
2. a kind of seedling root secretion collection device according to claim 1, it is characterised in that：In resin column tube（7）
The size of interior potting resin is the cm of 1.5 cm × 4.0.
3. a kind of seedling root secretion collection device according to claim 1, it is characterised in that：In watering can（5）Inside set
There is pressurizing valve（16）.
4. a kind of seedling root secretion collection device according to claim 1, it is characterised in that：In watering can（5）With spray
Head（6）The pipeline of connection is equipped with flow-limiting valve（13）.
5. a kind of seedling root secretion collection device according to claim 1, it is characterised in that：In resin column tube（7）
End equipped with sealing clamp（9）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721213942.3U CN207923478U (en) | 2017-09-21 | 2017-09-21 | A kind of seedling root secretion collection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721213942.3U CN207923478U (en) | 2017-09-21 | 2017-09-21 | A kind of seedling root secretion collection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207923478U true CN207923478U (en) | 2018-09-28 |
Family
ID=63611448
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721213942.3U Expired - Fee Related CN207923478U (en) | 2017-09-21 | 2017-09-21 | A kind of seedling root secretion collection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207923478U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109717213A (en) * | 2019-01-28 | 2019-05-07 | 广西大学 | A kind of disease-resistant tomato stock root exudates collection liquid and its suppressing method to ralstonia solanacearum |
| CN109717212A (en) * | 2019-01-28 | 2019-05-07 | 广西大学 | A kind of method that disease-resistant stock root exudates is collected and extracted |
-
2017
- 2017-09-21 CN CN201721213942.3U patent/CN207923478U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109717213A (en) * | 2019-01-28 | 2019-05-07 | 广西大学 | A kind of disease-resistant tomato stock root exudates collection liquid and its suppressing method to ralstonia solanacearum |
| CN109717212A (en) * | 2019-01-28 | 2019-05-07 | 广西大学 | A kind of method that disease-resistant stock root exudates is collected and extracted |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180928 Termination date: 20200921 |
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| CF01 | Termination of patent right due to non-payment of annual fee |