CN204085951U - A kind of sandy culture collects the device of secretions from plant roots - Google Patents

A kind of sandy culture collects the device of secretions from plant roots Download PDF

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Publication number
CN204085951U
CN204085951U CN201420621245.1U CN201420621245U CN204085951U CN 204085951 U CN204085951 U CN 204085951U CN 201420621245 U CN201420621245 U CN 201420621245U CN 204085951 U CN204085951 U CN 204085951U
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China
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secretions
plant roots
silica gel
pump
peristaltic pump
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CN201420621245.1U
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韩旭
曹振木
牛玉
刘维侠
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Tropical Crops Genetic Resources Institute CATAS
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Tropical Crops Genetic Resources Institute CATAS
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Abstract

Sandy culture collects a device for secretions from plant roots, and it relates to a kind of device collecting secretions from plant roots, is specifically related to the device that a kind of sandy culture collects secretions from plant roots.The utility model can not react the problem of plant root exudation situation really in order to the method solving existing collection secretions from plant roots.The utility model comprises a font support, culture tank, silica sand, inflator pump, glass wool, the first silica gel catheter, the first peristaltic pump, filtrator, vacuum pump, resin separating column, waste liquid returnable bottle, nutrition liquid bath, the second peristaltic pump, the second silica gel catheter, iron stand and multiple grass tube, and door font support is vertically arranged.The utility model is used for field of agricultural cultivation.

Description

A kind of sandy culture collects the device of secretions from plant roots
Technical field
The utility model relates to a kind of device collecting secretions from plant roots, is specifically related to the device that a kind of sandy culture collects secretions from plant roots, belongs to agriculture field.
Background technology
The material that secretions from plant roots comprises the decomposition product of the organism of plant tissue release, old and feeble epidermal cell and inclusions, root system is directly secreted and self product that soil microorganism is modified, be the important medium that Plants and Soils environment carries out material, energy and message exchange, the important content that systematic research is the research fields such as Auto toxicity, plant nutrient, soil ecology reparation is goed deep into it.Continuous cropping obstacle is prevalent in the plant production such as cereal crops, fruit tree, vegetables, flowers and medicinal material.Along with China population increases and the minimizing of cultivated area, and the undue pursuit to economic benefit, continuous cropping obstacle has become the major issue limiting agricultural development.The Auto toxicity that secretions from plant roots produces is the basic reason causing continuous cropping obstacle to occur, and therefore, the research of secretions from plant roots kind and effect receives the concern of more and more researcher.
Varied about the collection method of secretions from plant roots at present, mainly application is soil in-situ collecting method and water culture, and these two kinds of collection methods all exist drawback.The secretions from plant roots that soil in-situ collecting method is collected, the secretion adding soil microbial activity and interference, different soils is different with the situation of intervening root system with the activity of soil microorganism under climatic environment, therefore the root exudates collected is also different, the material that the root system of plant that this method can not be reacted really is secreted; The secretions from plant roots that water culture is collected can not react the situation of root exudates really, lacked mechanical resistance because root system of plant grows in the environment of water planting, the secretion difference that the material of secretion and plant cultivate generation in soil or matrix is very large.
Utility model content
The utility model is the problem that the method solving existing collection secretions from plant roots can not react plant root exudation situation really, and then proposes the device that a kind of sandy culture collects secretions from plant roots.
The utility model is the technical scheme taked that solves the problem: the utility model comprises a font support, culture tank, silica sand, inflator pump, glass wool, first silica gel catheter, first peristaltic pump, filtrator, vacuum pump, resin separating column, waste liquid returnable bottle, nutrition liquid bath, second peristaltic pump, second silica gel catheter, iron stand and multiple grass tube, door font support is vertically arranged, culture tank is arranged on a below for the crossbeam of font support, glass wool is laid in the bottom of culture tank, filled stone sand on glass wool, one end of first silica gel catheter is inserted in silica sand, the other end of the first silica gel catheter is connected with inflator pump, the inlet of the first peristaltic pump is connected with the liquid outlet bottom culture tank by a grass tube, the liquid outlet of the first peristaltic pump is connected with filtrator by a grass tube, filtrator is connected with vacuum pump by a grass tube, vacuum pump is connected with resin separating column inlet by a grass tube, the liquid outlet of resin separating column is arranged on the top of devil liquor recovery bottle, resin separating column is fixedly mounted on iron stand, one end of second silica gel catheter is inserted in silica sand, the other end of the second silica gel catheter is connected with the liquid outlet of the second peristaltic pump, the inlet of the second peristaltic pump is connected with the nutrient solution in nutrition liquid bath by pipeline.
The beneficial effects of the utility model are: the utility model can according to research need collect root exudates at any time, also the root exudates of plant different growing can be collected, all do not destroy the growth conditions of root system of plant, and fully can reflect the material that root system of plant is secreted naturally; The active principle that the flow velocity controlling waste liquid by piston can make root system secrete is enriched in macroreticular resin more fully; The utility model have simple to operate, acquisition time long, collect the period flexibly, collection efficiency high.
Accompanying drawing explanation
Fig. 1 is one-piece construction schematic diagram of the present utility model
Embodiment
Embodiment one: composition graphs 1 illustrates present embodiment, the device that a kind of sandy culture described in present embodiment collects secretions from plant roots comprises a font support 1, culture tank 2, silica sand 3, inflator pump 4, glass wool 5, first silica gel catheter 6, first peristaltic pump 7, filtrator 8, vacuum pump 10, resin separating column 11, waste liquid returnable bottle 13, nutrition liquid bath 14, second peristaltic pump 15, second silica gel catheter 16, iron stand 20 and multiple grass tube 9, door font support 1 is vertically arranged, culture tank 2 is arranged on the below of the crossbeam 1-1 of a font support 1, glass wool 5 is laid in the bottom of culture tank 2, filled stone sand 3 on glass wool 5, one end of first silica gel catheter 6 is inserted in silica sand 3, the other end of the first silica gel catheter 6 is connected with inflator pump 4, the inlet of the first peristaltic pump 7 is connected with the liquid outlet bottom culture tank 2 by a grass tube 9, the liquid outlet of the first peristaltic pump 7 is connected with filtrator 8 by a grass tube 9, filtrator 8 is connected with vacuum pump 10 by a grass tube 9, vacuum pump 10 is connected with resin separating column 11 inlet by a grass tube 9, the liquid outlet of resin separating column 11 is arranged on the top of devil liquor recovery bottle 13, resin separating column 11 is fixedly mounted on iron stand 20, one end of second silica gel catheter 16 is inserted in silica sand 3, the other end of the second silica gel catheter 16 is connected with the liquid outlet of the second peristaltic pump 15, the inlet of the second peristaltic pump 15 is connected with the nutrient solution in nutrition liquid bath 14 by pipeline.
Embodiment two: composition graphs 1 illustrates present embodiment, the lower surface that a kind of sandy culture described in present embodiment collects the crossbeam 1-1 of the door font support 1 of the device of secretions from plant roots is provided with illumination cultivation light 17.Other composition and annexation identical with embodiment one.
Embodiment three: composition graphs 1 illustrates present embodiment, the column 1-2 that a kind of sandy culture described in present embodiment collects the door font support 1 of the device of secretions from plant roots is provided with illumination cultivation light timer 18, and illumination cultivation light timer 18 is connected with the switch of illumination cultivation light 17.Other composition and annexation identical with embodiment two.
Embodiment four: composition graphs 1 illustrates present embodiment, the column 1-2 that a kind of sandy culture described in present embodiment collects the door font support 1 of the device of secretions from plant roots is provided with inflator pump timer 19, and inflator pump timer 19 is connected with the switch of inflator pump 4.Other composition and annexation identical with embodiment one.
Embodiment five: composition graphs 1 illustrates present embodiment, the lower end that a kind of sandy culture described in present embodiment collects the resin separating column 11 of the device of secretions from plant roots is provided with control valve 12.Other composition and annexation identical with embodiment one.
Principle of work
Culture tank 2 is made up of light-proof material, culture tank 2 is built with culture matrix silica sand 3, one deck glass wool 5 is laid in the bottom of culture tank 2, nutrient solution in nutrition liquid bath 14 injects culture tank 2 by silica gel catheter 16 under the effect of peristaltic pump 15, oxygen needed for plant growth passes in silica sand 3 by silica gel catheter 6 under the effect of inflator pump 4, duration of ventilation is controlled by inflator pump timer 19, peristaltic pump 7 is connected successively by grass tube in the bottom of culture tank 2, filtration unit 8 and vacuum pump 10, to flow in resin separating column 11 after root exudates suction filtration, flow in waste liquid instillation waste liquid returnable bottle 13 and speed is controlled by regulating the piston 12 of resin separating column 11 lower end, resin separating column 11 is fixed by iron stand 20, culture tank 2, illumination cultivation light 17, illumination cultivation light timer 18, inflator pump fixed pattern device 19 is fixed on support 1.
The method of collecting sand culture secretions from plant roots be by vernalization after vegetable seeds be directly seeded in and be equipped with in the culture tank 2 of silica sand 3, according to growth and development of plants need configuration nutrient solution in nutrition liquid bath 14, nutrient solution is injected culture tank 2 by peristaltic pump 15 and meets growth and development of plants needs, simultaneously by illumination cultivation light timer 18 and inflator pump timer 19 controlled light and inflationtime to meet growth and development of plants needs; By peristaltic pump 7, the root exudates of plant is transported to filtration unit 8, root exudates is made slowly to instill in resin separating column 11 by the suction filtration effect of vacuum pump 10, the rate of outflow regulating the piston 12 of resin separating column 11 lower end to control waste liquid makes root exudates effectively be enriched on macroreticular resin, collects waste liquid with waste liquid returnable bottle 13.

Claims (5)

1. the device of a sandy culture collection secretions from plant roots, it is characterized in that: the device that described a kind of sandy culture collects secretions from plant roots comprises a font support (1), culture tank (2), silica sand (3), inflator pump (4), glass wool (5), first silica gel catheter (6), first peristaltic pump (7), filtrator (8), vacuum pump (10), resin separating column (11), waste liquid returnable bottle (13), nutrition liquid bath (14), second peristaltic pump (15), second silica gel catheter (16), iron stand (20) and multiple grass tube (9), door font support (1) is vertically arranged, culture tank (2) is arranged on the below of the crossbeam (1-1) of a font support (1), glass wool (5) is laid in the bottom of culture tank (2), the upper filled stone sand (3) of glass wool (5), one end of first silica gel catheter (6) is inserted in silica sand (3), the other end of the first silica gel catheter (6) is connected with inflator pump (4), the inlet of the first peristaltic pump (7) is connected by the liquid outlet of a grass tube (9) with culture tank (2) bottom, the liquid outlet of the first peristaltic pump (7) is connected with filtrator (8) by a grass tube (9), filtrator (8) is connected with vacuum pump (10) by a grass tube (9), vacuum pump (10) is connected with resin separating column (11) inlet by a grass tube (9), the liquid outlet of resin separating column (11) is arranged on the top of devil liquor recovery bottle (13), resin separating column (11) is fixedly mounted on iron stand (20), one end of second silica gel catheter (16) is inserted in silica sand (3), the other end of the second silica gel catheter (16) is connected with the liquid outlet of the second peristaltic pump (15), the inlet of the second peristaltic pump (15) is connected with the nutrient solution in nutrition liquid bath (14) by pipeline.
2. a kind of sandy culture collects the device of secretions from plant roots according to claim 1, it is characterized in that: the lower surface of the crossbeam (1-1) of door font support (1) is provided with illumination cultivation light (17).
3. a kind of sandy culture collects the device of secretions from plant roots according to claim 2, it is characterized in that: the column (1-2) of door font support (1) is provided with illumination cultivation light timer (18), and illumination cultivation light timer (18) is connected with the switch of illumination cultivation light (17).
4. a kind of sandy culture collects the device of secretions from plant roots according to claim 1, it is characterized in that: the column (1-2) of door font support (1) is provided with inflator pump timer (19), and inflator pump timer (19) is connected with the switch of inflator pump (4).
5. a kind of sandy culture collects the device of secretions from plant roots according to claim 1, it is characterized in that: the lower end of resin separating column (11) is provided with control valve (12).
CN201420621245.1U 2014-10-25 2014-10-25 A kind of sandy culture collects the device of secretions from plant roots Active CN204085951U (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104749000A (en) * 2015-04-02 2015-07-01 上海交通大学 Device and method for extracting helophyte root exudates
CN105115780A (en) * 2015-08-24 2015-12-02 成都中医药大学 Plant rhizosphere soil and rhizosphere exudate collecting device and method
CN106769283A (en) * 2016-11-28 2017-05-31 常州大学 Experiment Root of Wetland Plants secretion continuously trapping system and continuous collection method
CN106989961A (en) * 2017-05-16 2017-07-28 江西省农业科学院土壤肥料与资源环境研究所 The collection method of plant root secretion collection device and xerophyte root exudates
CN110632219A (en) * 2019-08-14 2019-12-31 山东省农业可持续发展研究所 Root exudate collection and detection method
CN115606501A (en) * 2022-10-21 2023-01-17 西北大学 Tissue culture device for botany experiment

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104749000A (en) * 2015-04-02 2015-07-01 上海交通大学 Device and method for extracting helophyte root exudates
CN105115780A (en) * 2015-08-24 2015-12-02 成都中医药大学 Plant rhizosphere soil and rhizosphere exudate collecting device and method
CN105115780B (en) * 2015-08-24 2018-09-28 成都中医药大学 Plant rhizosphere soil and root rhizosphere secreta collection device and collection method
CN106769283A (en) * 2016-11-28 2017-05-31 常州大学 Experiment Root of Wetland Plants secretion continuously trapping system and continuous collection method
CN106989961A (en) * 2017-05-16 2017-07-28 江西省农业科学院土壤肥料与资源环境研究所 The collection method of plant root secretion collection device and xerophyte root exudates
CN110632219A (en) * 2019-08-14 2019-12-31 山东省农业可持续发展研究所 Root exudate collection and detection method
CN115606501A (en) * 2022-10-21 2023-01-17 西北大学 Tissue culture device for botany experiment

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