CN204882127U - Plant rhizosphere soil and rhizosphere secretion collection device - Google Patents
Plant rhizosphere soil and rhizosphere secretion collection device Download PDFInfo
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- CN204882127U CN204882127U CN201520641500.3U CN201520641500U CN204882127U CN 204882127 U CN204882127 U CN 204882127U CN 201520641500 U CN201520641500 U CN 201520641500U CN 204882127 U CN204882127 U CN 204882127U
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Abstract
The utility model discloses a plant rhizosphere soil and rhizosphere secretion collection device, observe box, collector, the native test tube of non - rhizosphere including the rhizosphere, rhizosphere observation box is equipped with vertical separation net including root system growth room and collection room between root system growth room and the collection room, and the collector is both ends open -ended hollow tube, and the both ends of collector all are equipped with seals net A, non - rhizosphere soil test tube both ends opening, and the native test tube one end of non - rhizosphere is equipped with closed net B. The utility model is suitable for an under the soil condition of banking up with earth, not disturbance plant roots, do not destroy plant rhizosphere environment and study the plant, cultivate the rhizospheric secretion of thing - absorption action process to different root system positions, different time dynamic monitoring analyses soil under realizing controllable condition again, obtain rhizosphere soil and rhizosphere secretion simultaneously.
Description
Technical field
The utility model relates to biological field, particularly relates to plant rhizosphere soil and root rhizosphere secreta gathering-device.
Background technology
At present, the collection of root rhizosphere secreta mainly concentrates on water planting, also has substrate culture collecting method and earth culture method.Rhizospheric environment under water culture and substrate culture method and the actual soil cultivation condition grown of plant and plant physiology situation have larger difference.Tradition earth culture method is all use distilled water drip washing after being taken out by root system of plant, and using leacheate as root rhizosphere secreta, in whole operating process, root system very easily sustains damage, and the inclusions of root system own and bleeding sap can impact the root rhizosphere secreta of plant own.Different with research object according to research purpose, some researchers devise special device to collect root rhizosphere secreta.As Chinese patent CN1963437A passes through a kind of root rhizosphere secreta continuously trapping system of zwitterion resin design, but design structure is more complicated, is not easy to place, and can only distinguish the root system secretion situation of shallow-layer and further portion.Chinese patent CN102776662A provides a kind of gathering-device of xerophyte root rhizosphere secreta, can only for the collection of xerophyte root rhizosphere secreta.Due to the root rhizosphere secreta of plant, there are differences in different positions and physiological periods, secrete laggard enter soil environment by the biology of rhizospheric environment and the impact of abiotic factor.All there is certain difference in the root rhizosphere secreta feature of these devices and plant reality.
Plant rhizosphere Environmental Studies, first will obtain Rhizosphere Soil sample, but actual differentiation Rhizosphere and nonrhizosphere is comparatively difficult, because root system character exists graded.Under general condition accurately distinguish root-Soil Interface, gather pedotheque, there is technical difficulty.The method of current acquisition Rhizosphere Soil mainly comprises field rhizosphere soil acquisition method and lab simulation cultivating method, and the former distinguishes Rhizosphere Soil and root face soil is too coarse, is difficult to show Rhizosphere Soil and the real situation of root system.The latter applies more, mainly comprise horizontal branch surface vessel cultivation, vertical root surface vessel cultivation, horizontal branch surface vessel cultivation designs proposition first by Kuchenbuck and Jungk (1982), root pad is formed in root system of plant bottom, after root pad contacts certain hour with its underpart soil, with microtome or the free-hand thick thin layer of 1mm that soil is cut into for analysis; Force (1984) etc. of restricting by the emperor himself uses the vertical root surface vessel cultivation improved first, measure the change of From The Rhizosphere of Rice nitrogen manure, method after improvement can select arbitrarily the root segment of different parts and the different growing of plant to carry out the research of root-Soil Interface nutrient transfer situation, but each obtained pedotheque is limited, the mensuration of entry can not be made simultaneously.
To sum up, these methods can only have certain reliability to carried out particular studies, have some limitations, and can not realize the secretion-absorption process of interference-free Dynamic Changes Analysis diverse location under controlled condition, different time plant rhizosphere.
Utility model content
The utility model aims to provide a kind of plant rhizosphere soil and root rhizosphere secreta gathering-device, be applicable under earth culture condition, work out not disturbance root system of plant, do not destroy rhizospheric environment, different root system position under realizing again controlled condition, secretion-absorption the action process of different time Dynamic Changes Analysis earth culture plant rhizosphere, obtains rhizosphere soil and root rhizosphere secreta simultaneously.
For achieving the above object, the utility model realizes by the following technical solutions:
Plant rhizosphere soil disclosed in the utility model and root rhizosphere secreta gathering-device, comprise rhizosphere observation box, gatherer, Non-rhizosphere soil test tube, described rhizosphere observation box comprises root growth room and collecting chamber, vertical separation net is provided with between described root growth room and collecting chamber, described gatherer is the hollow tube of both ends open, the two ends of gatherer are equipped with close-network A, and described Non-rhizosphere soil test tube both ends open, Non-rhizosphere soil test tube one end is provided with close-network B.
Preferably, described separation net, close-network A, close-network B are 300 object nylon wires, and described Non-rhizosphere soil test tube material is glass.
Further, described close-network A, close-network B all adopt cotton thread to fix, and described gatherer, Non-rhizosphere soil test tube are equipped with label.
Further, uniform osculum bottom described rhizosphere observation box.
Preferably, described rhizosphere observation box is transparent polypropylene box.
Preferably, described rhizosphere observation box is rectangle, long 45cm, wide 30cm, high 30cm, described osculum aperture 5mm, and described Non-rhizosphere soil test tube internal diameter 2cm, external diameter 2.5cm, long 10cm, described gatherer is glass tube, internal diameter 2cm, external diameter 2.5cm, long 5cm.
Further, the utility model also comprises polypropylene board, and described polypropylene board is parallel with separation net and split collecting chamber, and described gatherer is between separation net and polypropylene board, the two ends of gatherer contact with separation net, polypropylene board respectively, gatherer at least two.
The invention also discloses the collection method adopting plant rhizosphere soil and root rhizosphere secreta gathering-device, comprise the following steps:
Step 1, river sand are got the raw materials ready, and after river sand being crossed No. 3 sieves and No. 5 sieves, first clean with tap water, the watery hydrochloric acid of rear use 3% soaks one week, then after not having tart flavour, uses distilled water rinse with tap water, to pH=7, and dry for standby;
Step 2, load selected culture soil and experimental plant in root growth room, and insert Non-rhizosphere soil test tube, in collecting chamber, load clean river sand;
Step 3, in incubation, rhizosphere observation box being put into the artificial climate that imposes a condition indoor, near nylon wire, watering sterilized water or specific nutrient solution, for promoting the formation in vertical row root face;
Step 4, when the root system of plant in root growth room form vertical row root face in separation net side and just through separation net time, river sand in collecting chamber is dug out, put into the gatherer that specific soil is housed, be booked whole row's root face, one end is towards row root face, the other end polypropylene board cleaned pushes against, and the river sand got ready in remaining space step 1 fills up;
Step 5, particular growth phase plant, get towards the soil of the 2-5mm in row root face, in order to the analysis of mineral nutrient element in root rhizosphere secreta and Rhizosphere Soil, the other end fills identical soil.
Further, in step 2, in culture soil, clean cobblestone is added.
Preferably, in steps of 5, the described particular growth phase comprises florescence, fruit phase.
Compared with prior art, the utility model has following beneficial effect:
1, the utility model is applicable to the research that soil carries plant rhizosphere secretion-absorption process under training condition, can not obtain plant rhizosphere soil and root rhizosphere secreta by disturbance plant simultaneously, more can reflect the rhizospheric environment of plant under natural conditions.
2, the utility model is under the prerequisite ensureing plant normal growth, not disturbance root growth, does not destroy rhizospheric environment, avoids the root system damage that traditional Rhizosphere Soil acquisition methods causes, makes acquisition Rhizosphere Soil and root rhizosphere secreta by the interference of bleeding sap.
3, the utility model achieves under controlled condition, in different root system position, different vegetation period the rhizosphere secretion-absorption process of Dynamic Changes Analysis earth culture plant.
4, the material that the utility model is used is easy to get and can reuses, and manufacturing process is simple, and common lab all can be prepared, and cost is lower.
Accompanying drawing explanation
Fig. 1 is the utility model structural representation;
In figure: 1-rhizosphere observation box, 2-osculum, 3-separation net, 4-root growth room, 5-collecting chamber, 6-Non-rhizosphere soil test tube, 7-cultivating soil, 8-plant, 9-gatherer, 10-polypropylene board, 11-river sand.
Embodiment
In order to make the purpose of this utility model, technical scheme and advantage clearly understand, below in conjunction with accompanying drawing, the utility model is further elaborated.
As shown in Figure 1, plant rhizosphere soil disclosed in the utility model and root rhizosphere secreta gathering-device, comprise rhizosphere observation box 1, gatherer 9, Non-rhizosphere soil test tube 6, 1 uniform osculum 2 at the bottom of rhizosphere observation box, rhizosphere observation box 1 is transparent polypropylene box, rhizosphere observation box 1 comprises root growth room 4 and collecting chamber 5, vertical separation net 3 is provided with between root growth room 4 and collecting chamber 5, gatherer 9 is the hollow tube of both ends open, the two ends of gatherer 9 are equipped with close-network A, gatherer 9 has multiple, Non-rhizosphere soil test tube 6 material is glass, both ends open, one end is provided with close-network B, separation net 3, close-network A, close-network B all adopts 300 object nylon wires, close-network A, close-network B all adopts cotton thread fix and sew on label.
The utility model also comprises polypropylene board 10, and during use, polypropylene board 10 is parallel with separation net 3 and split collecting chamber 5, and gatherer 9 is between separation net 3 and polypropylene board 10, and the two ends of gatherer 9 contact with separation net 3, polypropylene board 10 respectively.
Concrete, make according to following size, rhizosphere observation box 1 is rectangle, long 45cm, wide 30cm, high 30cm, osculum 2 aperture 5mm, Non-rhizosphere soil test tube 6 internal diameter 2cm, external diameter 2.5cm, long 10cm, gatherer 9 is glass tube, internal diameter 2cm, external diameter 2.5cm, long 5cm.
The invention also discloses the collection method adopting plant rhizosphere soil and root rhizosphere secreta gathering-device, comprise the following steps:
Step 1, river sand are got the raw materials ready, and after river sand being crossed No. 3 sieves and No. 5 sieves, first clean with tap water, the watery hydrochloric acid of rear use 3% soaks one week, then after not having tart flavour, uses distilled water rinse with tap water, to pH=7, and dry for standby;
Step 2, load selected culture soil 7 and plant 8 in root growth room 4, in culture soil 7, add clean cobblestone, and insert Non-rhizosphere soil test tube 6, in collecting chamber 5, load clean river sand 11;
Step 3, in incubation, rhizosphere observation box 1 being put into the artificial climate that imposes a condition indoor, near nylon wire, watering sterilized water or specific nutrient solution, for promoting the formation in vertical row root face;
Step 4, when plant 8 root system in root growth room 4 form vertical row root face in separation net side and just through separation net time, river sand 11 in collecting chamber 5 is dug out, put into the gatherer 9 that specific soil is housed, be booked whole row's root face, one end is towards row root face, the other end polypropylene board 10 cleaned pushes against, and the river sand 11 got ready in remaining space step 1 fills up;
Step 5, particular growth phase plant, as florescence, fruit phase etc., get towards the soil of the 2-5mm in row root face, in order to the analysis of mineral nutrient element in root rhizosphere secreta and Rhizosphere Soil, the other end fills identical soil.
Certainly; the utility model also can have other various embodiments; when not deviating from the utility model spirit and essence thereof; those of ordinary skill in the art can make various corresponding change and distortion according to the utility model, but these change accordingly and are out of shape the protection domain that all should belong to the claim appended by the utility model.
Claims (7)
1. plant rhizosphere soil and root rhizosphere secreta gathering-device, it is characterized in that: comprise rhizosphere observation box, gatherer, Non-rhizosphere soil test tube, described rhizosphere observation box comprises root growth room and collecting chamber, vertical separation net is provided with between described root growth room and collecting chamber, described gatherer is the hollow tube of both ends open, the two ends of gatherer are equipped with close-network A, and described Non-rhizosphere soil test tube both ends open, Non-rhizosphere soil test tube one end is provided with close-network B.
2. plant rhizosphere soil according to claim 1 and root rhizosphere secreta gathering-device, is characterized in that: described separation net, close-network A, close-network B are 300 object nylon wires, and described Non-rhizosphere soil test tube material is glass.
3. plant rhizosphere soil according to claim 1 and root rhizosphere secreta gathering-device, is characterized in that: described close-network A, close-network B all adopt cotton thread to fix, and described gatherer, Non-rhizosphere soil test tube are equipped with label.
4. plant rhizosphere soil according to claim 1 and root rhizosphere secreta gathering-device, is characterized in that: uniform osculum bottom described rhizosphere observation box.
5. plant rhizosphere soil according to claim 1 and root rhizosphere secreta gathering-device, is characterized in that: described rhizosphere observation box is transparent polypropylene box.
6. plant rhizosphere soil according to claim 4 and root rhizosphere secreta gathering-device, it is characterized in that: described rhizosphere observation box is rectangle, long 45cm, wide 30cm, high 30cm, described osculum aperture 5mm, described Non-rhizosphere soil test tube internal diameter 2cm, external diameter 2.5cm, long 10cm, described gatherer is glass tube, internal diameter 2cm, external diameter 2.5cm, long 5cm.
7. plant rhizosphere soil according to claim 1 and root rhizosphere secreta gathering-device, it is characterized in that: also comprise polypropylene board, described polypropylene board is parallel with separation net and split collecting chamber, described gatherer is between separation net and polypropylene board, the two ends of gatherer contact with separation net, polypropylene board respectively, gatherer at least two.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520641500.3U CN204882127U (en) | 2015-08-24 | 2015-08-24 | Plant rhizosphere soil and rhizosphere secretion collection device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520641500.3U CN204882127U (en) | 2015-08-24 | 2015-08-24 | Plant rhizosphere soil and rhizosphere secretion collection device |
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| CN204882127U true CN204882127U (en) | 2015-12-16 |
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| CN201520641500.3U Withdrawn - After Issue CN204882127U (en) | 2015-08-24 | 2015-08-24 | Plant rhizosphere soil and rhizosphere secretion collection device |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105115780A (en) * | 2015-08-24 | 2015-12-02 | 成都中医药大学 | Plant rhizosphere soil and rhizosphere exudate collecting device and method |
| RU174973U1 (en) * | 2017-05-15 | 2017-11-14 | Николай Викторович Анисимов | Device for researching the development of plant roots by MRI |
| CN108519250A (en) * | 2018-04-18 | 2018-09-11 | 中国科学院水生生物研究所 | It is a kind of to collect submerged plant root exudates device and application method in situ |
| CN111133965A (en) * | 2020-02-18 | 2020-05-12 | 山东省农业科学院生物技术研究中心 | Peanut and corn intercropping root system separating and collecting device and planting method thereof |
-
2015
- 2015-08-24 CN CN201520641500.3U patent/CN204882127U/en not_active Withdrawn - After Issue
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105115780A (en) * | 2015-08-24 | 2015-12-02 | 成都中医药大学 | Plant rhizosphere soil and rhizosphere exudate collecting device and method |
| CN105115780B (en) * | 2015-08-24 | 2018-09-28 | 成都中医药大学 | Plant rhizosphere soil and root rhizosphere secreta collection device and collection method |
| RU174973U1 (en) * | 2017-05-15 | 2017-11-14 | Николай Викторович Анисимов | Device for researching the development of plant roots by MRI |
| CN108519250A (en) * | 2018-04-18 | 2018-09-11 | 中国科学院水生生物研究所 | It is a kind of to collect submerged plant root exudates device and application method in situ |
| CN108519250B (en) * | 2018-04-18 | 2024-02-27 | 中国科学院水生生物研究所 | Device for collecting submerged plant root secretions in situ and use method |
| CN111133965A (en) * | 2020-02-18 | 2020-05-12 | 山东省农业科学院生物技术研究中心 | Peanut and corn intercropping root system separating and collecting device and planting method thereof |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20151216 Effective date of abandoning: 20180928 |
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| AV01 | Patent right actively abandoned |